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Precursor Clone Collection
System Biosciences (SBI) has the largest
commercially-available collection of
microRNAs that are cloned into lentiviral
vectors. SBI’s constructs can be stably
expressed in a wide variety of cell types,
as opposed to synthetic microRNAs that
can only be transiently tested in cells.
Each construct in SBI’s collection consists
of the native stem loop structure and 200-400 base pairs of upstream and downstream � anking genomic
sequence. This unique feature ensures that the microRNAs expressed from SBI’s constructs will be correctly
processed in the cell into mature microRNAs.
Droshaprocessing
miR : miR*
dsRNA Intermediate
miR* separation
5’ 3’
5’3’
5’3’ 5’
3’
3’ 5’
miRISC complexes
DicerAGO2
AmpR
3’∆LTRWPRE
copGFPSV40 poly-A
cPPT
SV40 ORI
pUC ORI
RSV
CMV
EF1
5’LTRΨ
Dicerprocessing
microRNAPrecursors
microRNA Research
Lenti-miR™ microRNAPrecursor ClonesOverexpress Individual microRNAs or Pooled Virus Library
Highlights
• Largest collection of microRNA precursor clones available in lentivectors
• Native microRNA context ensures accurate and robust mature microRNA production
• Confi rm positive expressing cells with copGFP for convenient sorting of transfected or transduced cells
• Easily create stable cell lines
• High-throughput microRNA phenotypic screens using pooled Lenti-miR virus library
The microRNA precursors expressed from SBI’s Lenti-miR vectors produce mature microRNAs via the host cell’s processing machinery.
www.systembio.com/lentimir
5’ 3’
Native genomic sequences �anking hairpin (~400 bp)
Native microRNAhairpin structureretained.
SBI’s microRNAprecursor clonesare the real thing.
MCF-7 Cells infected (MOI 5:1) with Lentivirus made from PMIRH205PA-1 (pre-miR-205) construct. Images taken after 48 hours. Expression of mature miR-205 was validated and quantitated using qPCR assays.
0
2
4
6
8
10
12
miR-205 Control
Rela
tive
Expr
essi
on L
evel
(Nor
mal
ized
to U
6)
Mature miRNA Expression
MCF-7 Breast Cancer CellsTransduced with Lenti-miR-205
Phase Contrast GFP Fluorescence
Lentiviral Pre-miR-205 transduction
Lenti-miR™ microRNA Precursor Clones
Product Description Catalog # Size
MicroRNA Precursor Pooled Library(Pre-packaged) >10^7 IFUs/aliquot
PMIRHPLVA-1 4 virus aliquots
MicroRNA Precursor Pooled Library(HT, Pre-packaged) >10^7 IFUs/aliquot
PMIRHPLVAHT-1 10 virus aliquots
Pooled Lentivirus Particles of all SBI’s MicroRNA Precursors
Screen All microRNAs Simultaneously for Unique Phenotypes
SBI’s Lenti-miR virus library contains the pooled Lenti-miR precursor
clone collection in a ready-to-infect lentiviral preparation. The library
is designed for studying phenotypic e� ects associated with the over-
expression of individual microRNAs in hard-to-transfect mammalian
cell lines, primary cells, non-dividing cells and even whole animal
studies. The lentivirus preparation is pseudotyped with VSV-G for
broad cellular tropism. The microRNA or microRNAs responsible for
generating the phenotypes of interest may be recovered through
simple genomic PCR using lentivector-specifi c primers followed by
direct sequencing of microRNA precursor clones recovered.
Perform One Transduction and Easily Identify themicroRNAs Involved in Your Phenotypic Screen
Each virus within the pool will express an individual microRNA
precursor in its native context while preserving hairpin structures
to ensure biologically relevant interactions with endogenous
processing machinery and regulatory partners.
Identi�cation of microRNA E�ectors
Treatmentto inducephenotype
Selectionfor phenotype
Ampli�cationof microRNAe�ectors fromselected cells
InfectedTarget Cells
Treated Cells
Tumor InvasionCell Di�erentiationApoptosis
Transductionwith Lenti-miRmicroRNA Library
Pooled Lentiviral microRNA Screens
Simple Genomic PCR to Identify microRNA E�ectors
MicroRNA Precursor cloneCMV
5’ LVL Primer 3’ LVL Primer
copGFP
MicroRNAs
1000
500
(bp)M 16 29a 210 155 651 203 34a 125b
GFP
Flu
ores
cenc
ePh
ase
Cont
rast
HEK 293 Cells HT1080 Cells
Lentivirus Infection QS Data
We Also O� er Custom ServicesSystem Biosciences o� ers a wide-range of custom services to support your research, allowing you to spend less time making tools, and more time making discoveries. To learn more, visit our website at www.systembio.com/service or call us at 888-266-5066.
© 2009 System Biosciences, Inc. All rights reserved. System Biosciences and the System Biosciences logo are trademarks of System Biosciences, Inc.
System Biosciences, Inc.1616 North Shoreline Blvd.Mountain View, CA 94043
Toll Free: 888.266.5066Fax: 650-968-2277Email: [email protected]
The most comprehensive microRNA Precursor Clone Collection in lentiviral vectors available anywhere.