Upload
erick-tatro
View
217
Download
0
Embed Size (px)
Citation preview
8/10/2019 MicroRNA and Memory Deficits in Aging
1/13
MicroRNA Scientific InterestGroup
University of California San Diego
Erick [email protected]
First MeetingOctober 14, 2014
mailto:[email protected]8/10/2019 MicroRNA and Memory Deficits in Aging
2/13
4:00 - 4:05 Introduction
4:05 - 4:20 Study of MicroRNA and Memory
Deficits in Aging
4:20 - 4:50 Sketches of miRNA Regulation in T
and B Cells: Prospects for
Immunogenomic Therapy
4:50 - 5:00 Discussion
AGENDA
8/10/2019 MicroRNA and Memory Deficits in Aging
3/13
MicroRNA and Memory Deficits inAging
Erick T. TatroDepartment of Psychiatry, UC San Diego
October 14, 2014
8/10/2019 MicroRNA and Memory Deficits in Aging
4/13
Sam and Rose Stein Institute for Research on Aging
Young vs Aged Mouse
Some Aged Mice Exhibit Memory Deficit
BACKGROUND Model Mammal
ObjectDiscriminationRatio(DR)
0.0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
Young Old
ImpairedNormalNormal
Aged
Training Phase
Testing Phase
?
8/10/2019 MicroRNA and Memory Deficits in Aging
5/13
Mouse - TaqMan Low Densi ty Array (384 wells)
MIRNA ARRAY Hippocampus
-0.4
-0.3
-0.2
-0.1
0.0
0.1
0.2
0.3
0.4
Legend
miR-130b
miR-138
miR-222
miR-29b
miR-339-3p
miR-340-5p
miR-582-3p
Young Aged
NormalNormal ImpairedLog
(Fold
Control)Expression
Tatro & Scott et al. PLOS One(2010)
8/10/2019 MicroRNA and Memory Deficits in Aging
6/13
Validate & ImageRESULTSQ PCR
Young Old
ImpairedNormalNormal
In Situ Hybridization
MiR-138
Whole Brain
Hippocampus
Whole Brain
Hippocampus
Young
Aged /
Impaired
MiR-138
Aged
Exiqon Corporation
8/10/2019 MicroRNA and Memory Deficits in Aging
7/13
Target of miR138SIGNIFICANCE
S SH
APT1
APT1mRNA
miR-138
Palmitoylation of Proteins in Dendritic Spine
Stable Extend
Acyl Protein Thioesterase 1
8/10/2019 MicroRNA and Memory Deficits in Aging
8/13
Target of miR138RESULTS
APT1-mR
NA
-0.25
0.00
0.25
0.50
0.75
1.00 GroupYoung
Aged Unimpaired
Aged Impaired
Young Aged
NormalNormal Impaired
Q PCR In Situ Hybridization
Acyl ProteinThioesterase
Acyl ProteinThioesterase
Whole Brain
Dentate Gyrus
Hippocampus Aged
8/10/2019 MicroRNA and Memory Deficits in Aging
9/13
Serial SectionsRESULTS
miR-138 APT1 Protein
Dentate
Gyrus
Aged /
Impaired
Young
Tatro et al. Am J Geriatric Psych. (2013)
8/10/2019 MicroRNA and Memory Deficits in Aging
10/13
CONCLUSION Age associated memory impairment correlated
with lower miR-138 expression, particularly inthe hippocampus.
FURTHERSTUDY Examine miR - mediated syanptodendritic
changes. Examine causes of differential miR-138
regulation: epigenetics, transcription factors,
HPA axis homeostasis.
8/10/2019 MicroRNA and Memory Deficits in Aging
11/13
THANKYOU Department of Psychiatry
Cristian Achim, Ian Everall, Mariana Cherner, Svetlana Semenova, David Moore, IgorGrant, Hamp Atkinson
Eliezer Masliah, Erick Scott
Ben Soontornniyomkij, Virawudh Soontornniyomkij, Silke Nuber, Ben Guoux, MichaelYang, Intan Purnajo, Alex Yermanos
Sam and Rose Stein Institute for Research on Aging Dillip Jeste, Vicki Risbrough, Jared Young
UC San Diego Center for AIDS Research
Virology Core
Davey Smith, Sara Gianella, Doug Richman
Genomics Core
Christopher Woelk, Steffney Rought, Pinyi Du
HIV Neurobehavioral Research Programs
Exiqon Corporation (Small Grants Award for Supplies)
NIDA (R03DA031591) and NIMH
8/10/2019 MicroRNA and Memory Deficits in Aging
12/13
4:00 - 4:05 Introduction
4:05 - 4:20 Study of MicroRNA and MemoryDeficits in Aging
4:20 - 4:50 Sketches of miRNA regulation in T and
B cells: Prospects for immunogenomic
therapy
4:50 - 5:00 Discussion
AGENDA
8/10/2019 MicroRNA and Memory Deficits in Aging
13/13
MicroRNA Scientific InterestGroup
University of California San Diego
Erick [email protected]
First MeetingOctober 14, 2014
mailto:[email protected]