Microfluidic Characterization Project Presentation

Microfluidic Characterization Project Presentation

Microfluidic Characterization Project Presentation

By Yuval Levental

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Outline

Introduction

Fluigent Equipment

MFCS

Flowboard

M-Switch

Labview program:

Program 1

Program2

SciTe program

Program 1

Program2

Conclusion

Abstract

This project involved performing several experiments with Fluigent devices, LabView software, and the Fluigent script module.

I started out with the script module and programmed pressure settings, pressure increases, pressure decreases, wait periods, loops, flow settings, and switch settings.

In LabView, I learned how to visually program using draggable loops and sequence boxes.

The Fluigent devices I used were the pressure monitor, flow reader, and M-Switch.

Fluigent Equipment

The MFCS-EZ is the Microfluidic Flow Control System. It is a pressure-based flow system for micro-fluidic and nano-fluidic applications.

The maximum pressure value available is 7000 mbar, and it can control up to four reservoirs.

The pressure is piped into the Fluiwell reservoirs, which sends the fluid into thin straws. The pressures can be controlled through a software interface, a SciTE script, or a LabView module.

MAESFLO Layout

This is an example MAESFLO layout for the microfluidic control system (16 channel).

On most of the screen are sliders that can be moved up and down by the users to set the pressure level, along with the maximum limit.

3 channels (1 bar) and 1 channel (7 bars)

Flowboard Interface

The flowboard can measure and set the flowrates for the four channels.

The measurements are done through the flow unit devices.

The flowrates can be set through the flowboard program on the computer, which in turn adjusts the pressure on the MFCS. Calibration between the flowboard and MFCS is required.

M-Switch and 2-switch Configuration

The M-Switch is a device that takes inputs from the MFCS and outputs only certain inputs from a total of 10 maximum inputs.

The 2-switch outputs to the recover and waste filling vials.

The switchboard is connected to the MFCS, which is also connected to the M-Switch.

Experiments

Labview Programs

The two main components of the program are the Front Panel and the Block Diagram. The front panel contains all the visible inputs and outputs for the program. These include graphs, indicators, and counters.

The Block Diagram contains all of the internals of the program, showing the sequence and the order of components. In both cases, the components are chosen by right-clicking the background and selecting the proper components.

The two main loops are the for loop and the while loop. The for loop has a numerical iteration count and no stop condition. The while loop has no numerical iteration count and a stop condition.

Program 1: Count up then Down Program

This program increments pressures by 10 mbar for every given period length for a designated number of cycles, until a certain value is reached.

When this value is reached, the program will cycle down by 10 mbar until the total number number of loops has been met. This is achieved through the true/false triangle.

This is the user interface of the same program. Pressure 1 is the initial starting pressure, and Pressure 2 is the maximum added pressure desired.

Other inputs include the period in ms and the MFCS serial number. The graph displays the increasing and decreasing pressure. The array indexes the increasing and decreasing values.

Program 2: Flowrate Reader

This program accepts channel pressures as inputs and outputs the flowrates on an array.

Both the MFCS and flow reader are used in this case. The program is created by connecting several blocks together.

The program starts by initializing the command "Detect MFCS_EZ". Another command is used to output the Flowboard serial number and MFCS serial number.

The channel pressures which are inputted are read into the channels, and the Flow Rates are outputted.

The channel pressures are displayed on the left graph with respect to time.

The flowrates are displayed on the right graph.

SciTE Programs

The script always starts with the #include declaration. It is directly followed by the Init() sequence.

Other commands:

Pressure(X,Y,Z...) - set the pressures starting from Channel 1

Wait(t) - Time to wait in seconds

MSwX(n) - Set the M-switch to a specific position

End() - Finish the program

Load("MF_320_FRCM_0352698260799") - Required Identifictation file to set flowrates

Flowrate(X,Y,Z...) - set the flowrates starting from channel 1

Program 1: Flow script

The Flowscript is a very simple program which to begin with, loads an identification file that is necessary.

The program then waits 5 seconds, and Channel 1 is set to a flowrate of 20 ul/min.

The program runs until a volume of 50 ul is reached, then the flowrate of 0 ul/min is set and the program finishes.

For the Pressure Control script, the pressure for channel 1 is set to 500 mbars.

Ten seconds pass, the M-Switch is set to position 1, and five seconds pass. The M-Switch is set to position A, and sixty seconds pass. The first pressure is then set to zero, and the second pressure is set to 1000 mbars. The M-Switch is set to position 3.

Five seconds pass, and the M-Switch is set to position A. Sixty seconds pass, and the pressures are set to zero. One second passes, and the program terminates.

Program 2: Pressure script

The Flowscript is a very simple program which to begin with, loads an identification file that is necessary.

The program then waits 5 seconds, and Channel 1 is set to a flowrate of 20 ul/min.

The program runs until a volume of 50 ul is reached, then the flowrate of 0 ul/min is set and the program finishes.

For the Pressure Control script, the pressure for channel 1 is set to 500 mbars.

Ten seconds pass, the M-Switch is set to position 1, and five seconds pass. The M-Switch is set to position A, and sixty seconds pass. The first pressure is then set to zero, and the second pressure is set to 1000 mbars. The M-Switch is set to position 3.

Five seconds pass, and the M-Switch is set to position A. Sixty seconds pass, and the pressures are set to zero. One second passes, and the program terminates.

Conclusions

Personal Conclusion

This project lasted three months, from the beginning of October to the middle of January, with a two-week break in between. I worked in both ESIEE in a couple lab rooms, and in the Lavoisier lab at UPEM.

SciTE scripts were easy to program, as they resembled everyday language. However, the LabView programs were far more difficult, as they involved creating a multi-layered diagram.

I needed to figure out how to properly order the components, and which loop squares to use.

My main supervisor was Imaddeine Azzouz. We got along pretty well. Usually, we would meet through phone at a designated location.

I worked on the project 15-20 hours a week. Overall, I improved my communication, attendance, and writing skills.

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Visual

Simple

Everyday language

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Microfluidic Characterization Project Presentation