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Valencia CollegeMCB 2010C MICROBIOLOGY
Syllabus and schedule
Professor Nalini OdapalliOffice HSB117Phone 330 789 2824 (Cell) 407 582 1809 (Office) Email [email protected] COURSE DESCRIPTION This lecture/lab course is designed for Health Science majors. Survey of microbial forms with emphasis on bacteria and viruses: morphology, physiology, genetic mechanisms, and control of microorganisms. Pathogenic processes and microbes discussed in detail.PREREQUISITE Minimum grade of C in BSC 1010C or BSC 2093CPREREQUISITE NOTE If you do not meet the prerequisite requirements for this course, please
arrange for a conference with your instructor as soon as possible. It is not advisable to take BSC 2093C and MCB 2010C in the same term unless you have a strong science background.
TEXT Cowan, Marjorie K. Microbiology: A Systems Approach ISBN 1308344996, Custom Edition 5
LABORATORY MATERIALS Leboffe, Michael J. and Burton E. Pierce. Microbiology Laboratory Theory & Application, ISBN 1617314773, Edition 3 Additional lab materials - Lab coat placed in a gallon-sized freezer bag, disposable gloves that fit to the hand (minimum of two pairs per lab), black permanent ink marking pen, closed-toe shoes, and a hair tie-back if appropriate.
MIXED MODE CLASS From the experience of teaching several mixed mode courses; I can say that mixed mode course students have to be high in the discipline. The reason being the lectures are online and lecture tests have due dates. So it becomes totally your responsibility to take charge of learning the material and mastering the concepts before taking the lecture tests which are challenging. Your awareness should be high and in parallel to the rigor of this college-level course to be successful in this course.Every week before coming to class you must watch videos, fill pre-quiz sheets, and master the basics.Come to class to absorb and master the concepts.Make use of sample test suggestions to score well on the lecture tests.Make use of lab guides to do well in the lab quizzes and Lab tests. ASSESSMENT
Lecture Tests 500Lecture Quizzes 150Laboratory Quizzes 150Laboratory Tests (100 pts each) 200Total Points 1,000
GRADING SCALE
A 90 - 100 % B 80 - 89 % C 70 - 79 % D 60 - 69 % F 59 - 0 %
Note: Any uncompleted coursework will be assigned a grade of 0. Lecture Tests and the Final Lecture Test will cover the material presented from the point of the previous test. A lecture and or Lab quiz will be given once a week at the beginning of class. If you are late, you will
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not be given extra time. You must be present for the complete class for your grade to be counted. Leaving early would result in zero on the tests and quizzes given that day in the class.Laboratory Tests/Quizzes contain actual specimens collected from laboratory exercises. Answers to questions following each laboratory exercise are potential questions for lab tests and quizzes.Read labs before coming to lab. Lack of preparation or poor technique may cause a loss of up to 10 points from the laboratory work grade. In the case of repeated safety violations, a student may be asked to leave the lab and it will be an unexcused absence with zeroes on the tests/quizzes given. All tests and quizzes are timed. Each question only 1 minute.Lecture tests have 50 multiple choice questions and time 60 minutes.Lab tests can be multiple choice, blank and short essays, time 2 hours.Lecture and lab quizzes usually contain 10 multiple choice or fill in the blank questions, time 10 minutes. For mixed mode Classes, lecture tests and quizzes are given in the testing centers. You can take these on any Valencia campus. More details about testing center will be provided on Canvas.
All the lab quizzes and lab tests will be given in the class.You cannot use your books or notes for any test or quiz. None of my quizzes is open book.You have to know the material to take test and quizzes.
ATTENDANCE POLICY
WITHDRAWAL POLICY
In this class, a student missing 4 hours of class time or who has 3 late entries or 3 early exits will be withdrawn from the class. Before withdrawing from the class you are advised to consult the professor and an advisor or counselor. Absences will be excused with proper documentation of an unexpected life event. If you have perfect attendance, a 69, 79 or 89% following the final test, your grade will be rounded up but otherwise, no rounding will occur. “A student who withdraws from a class before the withdrawal deadline will receive a grade of “W.” A faculty member is permitted to withdraw a student from the class up to the beginning of the final test period, for violation of the faculty member's attendance policy, as published in the faculty member's syllabus. A student is not permitted to withdraw from this class after the withdrawal deadline; if you remain in the class after the withdrawal deadline, you can only receive a grade of A, B, C, D, F or I. An I grade will only be assigned under extraordinary circumstances that occur near the end of the semester. If you receive an I, the work missed must be made up during the following semester, at which time you will get an A, B, C, D or F. Failure to make up the work during the following semester will result in you getting a grade of F in the course. Any student who withdraws from this class during a third or subsequent attempt in this course will be assigned a grade of “F.” (Valencia Policy 4-07 Academic Progress, Course Attendance and Grades, and Withdrawals). In order to academically maintain financial aid, students must meet all of the following requirements:
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FINANCIAL AID
CLASSROOM RULES
ELECTRONICS
Complete 67% of all classes attempted, and Maintain a Valencia GPA of 2.0 or higher, and Maintain an overall GPA of 2.0 or higher, and Complete degree within the 150% timeframe
Detailed information about maintaining satisfactory academic progress (SAP) can be found at http://valenciacollege.edu/finaid/satisfactory_progress.cfm
No Food or Drink in the Lecture or Lab Rooms.
The Student Code of Conduct is published in the Valencia Student Handbook. Violation of any of these rules may lead to disciplinary action and authorized penalty.
No side discussions during lecture. It is considered as a distraction to learning.
No walking in and out of the classroom/lab frequently.Be on time and stay until the end of the class for complete creditIf you have questions, then raise your hand and ask professor not your neighbor No electronics allowed except laptop computers for note-taking and cell phones for recording lectures. Recording lectures are allowed for additional reference but it is not a replacement for the lecture.Recording privilege can be retracted if a student is inattentive during lectures.Cell phones should be silent, no texting during class.
Generally, cell phones are out of sight for the duration of lecture, tests and test reviews.
Keep the laptop usage limited to note taking only.
Unnecessary use of devices in the class is prohibited.
Your devices can be inspected during lectures if the student is not paying attention to the lecture.Upto hundred points are deducted the first time a student uses a cell phone or other device in the classroom. The second time a serious action is taken after meeting Dean. Please read ‘Dean’s letter’ at the end of the syllabus.TEXTING: Text messaging during class is distracting to others and does not allow the student to be mentally attentive. A student using cell phone will be asked to leave the classroom and extra credit will be zeroed. Cell phones should not be handled at all during lab and should be turned off and put away due to safety concerns.
As many tests and quizzes are online- you are required to bring laptop or iPad. If you don’t own one, then you can borrow from our library. Contact library regarding the lending procedure.
Make-up policy: You cannot make up tests, quizzes, extra credit or lab test whether you arrived late or were absent. Everyone is given one extra credit opportunity on Canvas to make up for any missed quiz or test. DISCLAIMER STATEMENT
Changes in this syllabus and/or schedule may be made at any time during the term by notice of the professor. You will be notified of changes in class or email or Canvas, at least 24 hours in advance. It
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is your responsibility to stay informed of any changes. You are required to keep all returned graded work for two weeks after a final grade has been issued to ensure that no grade discrepancies have occurred. This syllabus is a contract between the student and the instructor. By attending this course, the student agrees to and accepts the terms and conditions of this contract. It is the responsibility of the student to carefully read this syllabus/contract and to adhere to all policies and procedures within.
ACADEMIC HONESTY All forms of academic dishonesty are prohibited at Valencia College. Academic dishonesty includes, but is not limited to, plagiarism, cheating, furnishing false information, forgery, alteration or misuse of documents, misconduct during a test situation, and misuse of identification with intent to defraud or deceive.Academic penalties may include, without limitation, one or more of the following: loss of credit for an assignment, examination, or project; withdrawal from the course; a reduction in the course grade; or a grade of “F” in the course. If a student is found cheating, notification will be submitted to the Dean of Science and record of the violation will be added to your permanent transcript. During tests, all cell phones and electronic devices should be turned off and stored away in purses or backpacks. Book bags, backpacks, purses, notes, drinks and/or food are not allowed in the testing area. You will need only a pencil and eraser. Students will not be permitted to leave the room and come back to finish their test under any circumstances unless medical documentation has been submitted. STUDENT CONDUCT By registering at Valencia Community College, a student assumes the responsibility for becoming familiar with and abiding by the general rules of conduct. Violation of any of these rules may lead to a disciplinary warning, probation, suspension, expulsion, or other appropriate and authorized penalty. The Student Code of Conduct is published in the Valencia Student Handbook.COLLEGE POLICIES A full description of all College policies can be found in the College Catalog at http://www.valenciacollege.edu/catalog/ ; Policy Manual at http://www.valenciacollege.edu/generalcounsel/; and the Student Handbook at http://valenciacollege.edu/studentdev/CampusInformationServices.cfm STUDENT ASSISTANCE PROGRAM Valencia College is interested in making sure all our students have a rewarding and successful college experience. For that purpose, Valencia students can get immediate help that may assist them with psychological issues dealing with stress, anxiety, depression, adjustment difficulties, substance abuse, and time management, as well as relationship problems dealing with school, home or work. Students have 24-hour unlimited access to the BayCare Behavioral Health’s confidential student assistance program phone counseling services by calling (800) 878-5470. Three free confidential face-to-face counseling sessions are also available to students.STUDENT SERVICES Tutoring Center: Bldg. 7- 240 (Phone: 407 582 1633)
Testing Center: Bldg. 7- 217 (Phone 407 582 1323)Please note that tutoring services are free and are available at all Valencia campuses.STUDENTS WITH DISABILITIES Students with disabilities who qualify for academic accommodations must provide a letter from the Office for Student with Disabilities (OSD) and discuss specific needs with the professor, preferably during the first two weeks of class. The Office for Students with Disabilities determines accommodations based on appropriate documentation of disabilities (SSB 102. Phone 407 582 1523. West Campus). In addition to specific microbiology objectives, the course will also reinforce the following competencies wholly or partially:
VALENCIA COMMUNITY COLLEGE STUDENT COMPETENCIES1. Think – Think clearly, critically, and creatively. Analyze, synthesize, integrate, and evaluate in many domains of human inquiry.2. Value – Make reasoned value judgments and responsible commitments.
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3. Communicate – Communicate with different audiences using varied means.4. Act – Act purposefully, reflectively, and responsibly.LEARNING COURSE OUTCOMESStudents will be able to identify the major milestones in microbiology.Students will be able to describe how macromolecules contribute to the functions necessary for microbial life.Students will be able to classify microorganisms according to a common taxonomic hierarchy.Students will be able to differentiate between prokaryotic and eukaryotic organisms and explain the function of each structure in both cell types.Students will gain an understanding of viruses by explaining the viral structure, multiplication, spread, and control.Students will be able to explain basic microbial metabolism and summarize what is needed for the growth of microorganisms.Students will be able to describe all the chemical and physical means available to control microbes and to identify those that provide sterility.Students will be able to identify the mechanism of action of commonly used antimicrobial drugs and the concerns associated with the development of drug-resistant strains.Students will model their knowledge of molecular genetics and recognize the importance of basic genetic engineering.
10. You will be introduced in to a lecture to all the advanced molecular biology methods.11. You will be introduced to all the lab culture methods for growing, identifying and inspecting the bacteria.12. Students will be able to demonstrate the appropriate use of epidemiological terminology in the context of
public health.13. Students will be able to identify the mechanisms of pathogenicity and distinguish the different levels of
immunity operating in the human body.14. Students will be able to identify the causes of major human infectious disease and relate that information to
the appropriate organ system.15. Students will demonstrate a working knowledge of aseptic technique.16. Students will demonstrate an understanding of microscopy.17. Students will integrate their lab knowledge to identify unknown microbes and determine how appropriate
antimicrobial drugs are chosen for given bacterial infections.
STUDY TIPS“LEARNING IS AN ACTION VERB!! Most students need to do more than just sit through lectures and reread their notes. Spend 1-2 hour blocks of time EVERY DAY actively writing or discussing concepts to make them a part of your memory. Use the words you learn often, they will sink in better.Here are some study and classroom management tips that have assisted former students:
- Attend class daily and don’t be tardy. The introduction to each lecture explains the purpose of the entire lecture. Students who follow this rule won’t miss important information.- Fill up pre-quiz sheet before that chapter is discussed in class. This helps you to understand the concepts better and ask questions relevant to lecture tests.
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- REWRITE YOUR NOTES soon after the lecture; if the instructor permits, tape record lectures and replay to refresh your memory when you rewrite your notes.- Create flash cards with questions you make up from the lecture and lab with answers on the back.- Use mnemonic devices and other games to remember concepts; go to Google images, YouTube and Khan Academy for additional pictures and videos to clarify concepts.- Make lists of confusing topics from your studying and ask questions.- Take advantage of the professor’s office hours.- JOIN A STUDY GROUP and predict what questions the professor could ask on the test.- Get the telephone number of one or more buddies in case you are absent from a class.- Keep checking your grades on the Canvas and motivate yourself to do better.- Prepare your own dictionary of words which includes both scientific terms and general vocabulary wordsFor Lecture test: 1. Study PowerPoint while reading the chapters of the textbook. Do not ignore PowerPoints or Textbook, both are important.2. Study pre-quiz and test yourself again3. Use practice tests available on the McGraw-Hill textbook website4. Make sure you review class notes and your practice tests for achieving high score5. Lecture test questions are not easy but when you prepare well using the quizzes, online quizzes, and practice tests then it is possible to ace these tests.6. After the first test: See me if you got less than A on your first test. You will fill up a questionnaire which will help us see ways to improve score in the coming tests. I have tips to help you improve your grades, such as which tests and assignments are easy scorers, how to study for next test, how to increase memory, types of distractions and how to avoid them, etc. There is CARE program in Valencia college for helping you in many different ways.Weather Emergencies: In the case of weather emergencies, you can find out about school closings by signing up to update your information for Valencia Alerts on Atlas.
Absent: When you miss a class, it is your responsibility to get all the material missed, due dates of the quizzes, assignments dates upon return to class. This rule is applied to both excused and unexcused absences. Make sure you have taken 3 classmates contacts and ask all of them individually and learn everything you missed. This rule is applicable even to those who missed the very first class. A professor is not responsible to update you.
Seating: You will be placed in different groups every time and will be asked to change seats based on the need for the learning activities. Come with an open mind, flexible and humble personality with the mindset aimed at learning maximum from every class. Every moment in class is a precious learning moment.
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Drifting: Some students have the habit of constantly checking cell phones. This is the biggest distraction of this century. I want my students to learn and learn only during class time. If you are not in agreement with this rule please take a decision and chose another class.
Pop quizzes: You will be given several chances to score extra credit points. Always be alert in class to score maximum extra credit points. These cannot be made up if you missed a class.
Flight to catch: You already know the class schedule. Please do not schedule travel, ride to the airport, pick up and drop off that might interfere with class times.
Big Events: If you have weddings, birthdays, cruise, vacation preplanned which might interfere with our class then please withdraw the first week.
To Do list: It is your duty to copy and maintain a log of “To Do” list written on the board. This list contains a chain of events and due dates of quizzes/tests coming up. It also gives you basic information to make a study plan every week.
What to buy: Textbook, Lab manual from West Valencia bookstore, 2 composition notebooks, 4 pencils, 4 packets of 882-E scantron.
What to bring: Carry textbook, lab manual, scantrons, pencils, erasers, lecture notes and pens to every class and lab. Borrowing scantrons is not allowed once quiz begins
Etiquettes: High etiquettes are expected from each and every student in the class. Always place bags and bag tags away from walkways. Respect every class member and pay complete attention during lectures, presentations, questions and answer sessions.
Extra Assessments: Bioreadiness assessment, GenED critical thinking assessment, Faculty evaluation survey. Complete these and turn in during the last week of the semester to gain 5 extra credit points. Whenever deemed these assessment links will be emailed to you by the college and or professor.
I encourage you all to use media to post your evaluations such as ratemyprofessor website. Convey what is true about the course, college, learning experience, and the teacher. Evaluations are an important source of information for future students. I do not appreciate either negative views or positive things. Negative evaluations are detrimental to our work and positive evaluations are embarrassing for me. What we want is a genuine reporting of what’s done, what is the value of what we have done and let the readers decide.
Microbiology Schedule: Fall 2018 Dates will be announced on Canvas
Lecture
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Introduction – Ch. 1Tools of the Lab – Ch. 3
Bacteria and Archaea – Ch. 4Eukaryotes – Ch. 5Viruses – Ch. 6; Quiz in Chap 6LECTURE TEST 1: Ch. 1; 3-5Genetics – Ch. 9Genetic Engineering – Ch. 10
Controlling Microbes – Ch. 11Antibiotics – Ch. 12LECTURE TEST 2: Ch. 9-12MIDTERM LAB PRACTICAL TEST WEEKMaterials come from laboratory experiments performed during weeks 1-5Infection and Disease – Ch. 13Nonspecific Defenses – Ch. 14Specific Defenses – Ch. 15Disorders in Immunity – Ch. 16LECTURE TEST 3: Ch. 13-16Diagnosing Infections – Ch. 17Diseases of the Skin/Eyes – Ch. 18
Nervous System Disease – Ch. 19
Cardio/Lymph Disease –Ch. 20 (Quiz)
LECTURE TEST 4: Ch. 17-19Respiratory Disease - Ch. 21Digestive System Disease– Ch. 22Genitourinary Diseases – Ch. 23Genitourinary Diseases – Ch. 23Lecture Test 5: Ch 21-23 FINAL LAB TESTTest on lab experiments performed during weeks 6-12. Place and time will be confirmed later
VALENCIA COLLEGE LABORATORY RULES
West Campus Prevention is the key to a safe and effective laboratory experience.
General Safety Policies1. Always follow all written and verbal instructors’ directions. If you do not understand a direction or part of a
procedure, ask the instructor before proceeding.2. No working in the lab without your instructor present.3. No food or drink in the lab. No candy or gum in the lab.4. Do not apply cosmetics in the lab.5. No smoking in the lab.
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6. Conduct only authorized experiments. Never do anything in the laboratory unless it has been explicitly called for in the laboratory procedures or by your instructor.
7. Be prepared for your work in the laboratory. Read all procedures thoroughly before entering the laboratory. 8. Never fool around in the laboratory. Horseplay, practical jokes, and pranks are dangerous and prohibited.9. Observe good housekeeping practices. You must maintain an uncluttered area at all times. You must hang
purses, backpacks, coats in the space provided and keep aisles between workbenches free. Bring only your laboratory instructions, worksheets, and/or reports to the work area.
10. Keep aisles clear. Push your chair under the desk when not in use.11. Know the locations and operating procedures for all safety equipment:
a. Fire blanket, eyewash station, and safety shower d. Fire extinguisherb. Know where the fire alarm and exits are located e. First Aid Kitc. Spill absorbent and neutralization materials
12. Always work in a well-ventilated area. Use the fume hood (or snorkels) when working with volatile substances or poisonous vapors. Never place your head into the fume hood.
13. Notify the instructor immediately of any unsafe conditions you observe.14. Dispose of all chemical and biological waste properly. Never mix chemicals in sink drains. Sinks are to be
used only for water and those solutions designated by the instructor. Solid chemicals, metals, filter paper, and all other insoluble materials are to be disposed of in the proper waste containers, not in the sink. Check the label of all waste containers twice before adding your chemical or biological waste to the container.
15. Read carefully all labels and equipment instructions before use. 16. Keep hands away from face, eyes, mouth, and body while using chemicals. Wash your hands with soap and
water after performing all experiments. Clean and rinse your lab station at the end of the lab period. Keep all equipment clean.
17. Experiments must be personally monitored at all times. You will be assigned a laboratory station at which to work. Do not wander around the room, distract other students, or interfere with the laboratory experiments of others.
18. You may not enter the prep room area, other than in an emergency situation, without special advance permission.
19. Unauthorized visitors are not allowed.20. Inform your instructor if you have any medical condition (including pregnancy) that may compromise your
safety in the laboratory. This can be done in a private meeting between you and your instructor. Ask your instructor for a list of chemicals used in the experiments to help determine in which experiments you may participate. Show the list to your physician and follow his/her recommendations.You may be removed from the lab for inappropriate behavior!
Eye protection/Clothing21. For Chemistry and Microbiology laboratories, safety goggles are required. Other courses may require
occasional use of goggles. Goggles are available in the lab or you may bring your own.Goggles must meet ANSI Z87+D3 standards, protects against splash and high impact.
22. Contact lenses, especially soft lenses, can trap irritating vapors against the eye. Contact lenses should not be worn in the laboratory.
23. Dress properly during a laboratory activity: wear full-coverage clothing that covers shoulders, chest, abdomen, and legs to the knee or lower. Tie back long hair. Avoid clothing items that dangle.
24. You must wear shoes that fully enclose your feet: no sandals, flip-flops, or open-heeled slides are allowed in the laboratory rooms.
25. BSc1010c requires Knee-length lab coat.You will not be allowed into or remain in the lab if wearing inappropriate shoes or clothing.
Accidents and Injuries26. Report all accidents (spills, breakage, etc.) or injuries (cuts, burns, etc.) to the instructor immediately
regardless of severity. In the event of a true emergency, call 911after advising the instructor. In case the instructor is injured and unable to call 911, do not hesitate to dial 911 for emergency assistance. Campus security may be reached at 407-582-1000.
27. If a chemical should splash in your eye(s) or on your skin, immediately flush with running water from the eyewash station or safety shower for at least 15 minutes. Notify the instructor immediately.
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Handling Chemicals28. All chemicals in the laboratory are to be considered dangerous. Do not touch, taste, or smell any chemicals
unless specifically instructed to do so. You must avoid or minimize skin contact with all chemicals (gloves are available). The proper technique for smelling chemicals fumes will be demonstrated to you.
29. Check the label on chemical bottles twice before removing any of the contents. Take only as much chemical as you need and transfer to a labeled container. Keep caps on reagent bottles. Never switch caps.
30. Never return unused chemicals to their original containers.31. Never use mouth suction to fill a pipette. Use a rubber bulb or pipette pump.32. When transferring reagents from one container to another, hold the containers away from your body.33. Handle flammable hazardous liquids over a pan to contain spills. Never dispense flammable liquids
anywhere near an open flame or source of heat.34. Never remove chemicals or other materials from the laboratory area.35. Take great care when transferring acids and other chemicals from one part of the laboratory to another. Hold
them securely and walk carefully.36. Always know the hazards and physical and chemical properties of the materials used. Safety Data Sheets
(SDS) (formerly called Material Safety Data Sheets (MSDS)) are located in the prep areas and are available by asking the instructor. There are also many sources available on the internet.
Handling Glassware and Equipment37. Never handle broken glass with your bare hands. Use a brush and dustpan to clean up broken glass. Place
broken or waste glassware in the designated glass disposal container. Examine glassware before each use. Never use chipped or cracked glassware. Never use dirty glassware.
38. Before unplugging electrical equipment, turn equipment off. When removing an electrical plug from its socket, grasp the plug, not the electrical cord. Hands must be completely dry before touching an electrical switch, plug, or outlet. Do not use the damaged electrical equipment.
Heating Substances39. Be cautious with flames and flammable solvents. Keep bags, purses, and papers away. 40. Never leave a lit burner unattended. Never leave anything that is being heated or is visibly reacting
unattended. Always turn the burner or hot plate off when not in use.41. Do not place hot crucible directly on the laboratory desk. Always use an insulating pad. Allow plenty of time
for hot apparatus to cool before touching it.Alternative Arrangements for Pregnant Students:
The laboratory environment often times will involve the use of and/or exposure to chemicals or other substances. If you are pregnant and concerned about your exposure to these chemicals, please see your instructor, lab manager, or Dean to discuss possible alternative arrangements. Students are also invited to contact Mr. Ryan Kane, Title IX Coordinator/Equal Opportunity Officer, 407-582-3421, [email protected], regarding requests for alternative arrangements relating to pregnancy.Disclaimer: At times, your experiments may require additional safety measures not mentioned here. Follow all instructions given by your instructor in these situations.
Laboratory Safety Contract: Student copy
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I, ___________________________________ (student’s name) have read, understand and agree to follow all the laboratory rules set forth in this contract. To ensure my own safety as well as that of other students and instructors in my class, I understand that I must obey these rules, as well as additional verbal and written procedures set forth by my instructor. I understand that the laboratory can be a dangerous place and that following these laboratory rules is critical to my continued participation in lab activities. By agreeing to follow this Laboratory Safety Contract, I understand that my failure to follow the laboratory rules may result in serious consequences, including but not limited to a reprimand, removal from the course, student conduct proceeding, or another discipline as appropriate.
I understand that I am responsible for determining whether any medical conditions I may have, including pregnancy, will compromise my safety in the lab. It is my responsibility to ask my instructor for a list of chemicals used in the experiments and to consult my physician for his or her recommendations about my participation.
__________________________________
Student Signature Note: A parent or legal guardian signature is required below for any student under the age of 18 years.
_____________________
Date
Instructor _____________________
Course Name _________________
Course Number________________
CRN#_____________
Lab Room___________
For the Parent or Guardian (if under 18 years old):
I have read and reviewed the laboratory rules with my child and ensured that he/she understands them. I understand the behavior that is expected of my child and will ensure that he/she complies with the laboratory rules and any directives of the instructor.
__________________________________
Parent/Guardian Signature for minor
_______________________
Date
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What does Title IX say about pregnant & parenting students?
http://www2.ed.gov/about/offices/list/ocr/docs/pregnancy.pdf
Title IX specifically prohibits discrimination against a student based on pregnancy, childbirth, false pregnancy, termination of pregnancy, or recovery from any of these conditions.
It is illegal for schools to exclude a pregnant student from participating in any part of an educational program.
Schools may implement special instructional programs or classes for a pregnant student, but participation must be completely voluntary on the part of the student, and the programs and classes must be comparable to those offered for other students.
A school must excuse a student’s absences because of pregnancy or childbirth for as long as the student’s doctor deems the absences medically necessary. When a student returns to school, she must be allowed to return to the same academic and extracurricular status as before her medical leave began.
An instructor may not refuse to allow a student to submit work after a deadline that she missed because of absences due to pregnancy or childbirth. If a teacher’s grade is based in part on attendance or participation, the student should be allowed to earn the credits missed.
A school may require a pregnant student or student who has given birth to submit medical certification for school participation only if the school also requires such certification from all students with physical or emotional conditions requiring the attention of a physician.
Harassment of a pregnant student could be a violation of Title IX. This includes peer or faculty to student harassment such as verbal name-calling, graphic or written statements i.e. jokes, rumors, sexual propositions, etc.
Institutions are required to provide assistance such as adjustments to the regular program that is reasonable and responsive to a student’s temporary pregnancy status i.e. larger desk, frequent trips to the bathrooms, etc.
Institutions are expected to give the student an opportunity to make up any work missed. Alternatives such as retaking a semester, taking part in an online course, or allowing the student to have additional time in a program or class to continue at the same pace and finish at a later date. The student should be allowed to choose how to make up the work.
If you have any Valencia College community members who have questions regarding this topic, please feel free to contact, or suggest that the student, faculty or staff member contact the College’s Title IX Coordinator/EO Officer at 407-582-3421.
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LETTER FROM THE DEAN
Expectations of a Valencia College Science Student
Welcome to the Science Division of Valencia College’s West Campus; we are enthused to have this opportunity to assist you in achieving your educational goals. Higher education is a privilege and an opportunity; it is your responsibility to realize that you are in control of those behaviors and actions that can enable success in this course.
From your professors, you can expect the following:1. They will be prompt, courteous and respectful. 2. They will provide a professional learning environment throughout the entire scheduled instructional period.3. They will provide an up-to-date syllabus; when changes need to be made, they will announce changes in a manner
that is conveyed to all students in their class.4. They will provide you with a course that is of necessary rigor to prepare you for the career you have chosen;
because the class you are taking transfers to a four-year college or university, it will be taught with the same academic expectations. The bonus of starting your education at Valencia, when compared to attending a four-year college, is you will be able to get more personal attention with small class sizes and you will be able to save money; the classes are not easier.
5. They will offer time outside of class to discuss your questions.6. They will be available to discuss your class concerns in private, outside of class time; you should try to resolve your
class concerns with your professor before you try to voice your concerns with administrators.7. They will respond to emails within 2 business days.8. They will expect from you what they have expected from the many students who passed their classes in the past.
In return, this is what your professor will expect from you:1. You will be prompt, courteous and respectful.
Prompt means you come to class on time, ready to work, with all assignments completed before you enter class or lab. It means that you have studied, that all conversations, texting, and diversions come to a stop and that you are ready to contribute to a positive learning environment from the beginning of the class or lab.
Courtesy means you are polite in your attitude and behavior towards your professor and classmates.
Respectfulness means that you regard everyone’s classroom experience as valuable to them, that you accept your professor’s expertise and experience to create relevant course objectives and that you treat college and personal property with care.
2. You will read your syllabus; your syllabus is a contract between your professor and you. Be aware of deadlines to complete assignments on time and know your professor’s attendance policy. You will need to attend class for the full length of time allotted to your science class and lab.
3. You will be aware of Valencia College policies and procedures found in the college catalog; ignorance of policies and procedures doesn’t mean they don’t apply.
4. You will be serious about learning; it is your responsibility to be an active participant in your own learning. You will need to devote sufficient time to learn the material presented by your professor; for most students, this means you will need to spend at least two hours studying for every hour of instruction. Learning is an action verb; you will need to do more than sit through class and reread your notes to be successful. To succeed in higher level classes, you must retain the information, concepts and skills you will learn; this can only happen if you work at learning to make the course content a part of your long-term memory. Do not cram!!
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5. You will be encouraged to produce your own study guides. Most college professors do not provide study guides, but they can give you tips that will help you produce your own study guides to gain a better understanding of the course content.
6. You will be expected to participate fully in classroom activities. The work you produce must be your own; cheating in any form is not tolerated. When you are caught cheating in class or after test then you will be given a zero on the test and withdrawn from the class.
7. You will be expected to contribute to a positive learning environment. Avoid classmates who speak negatively, or who have a negative outlook, about your class or your professor. Instead, get to know your professor during office hours; you will learn much more with a positive attitude.
8. You will be held to a high standard of maturity and responsibility. Disruptive behaviors will not be tolerated in the classroom or lab. First-time disruptions will be handled by your professor and may include a request that you leave the class or lab. Very serious or repeated disruptions will be reported, to the Dean of Science and the Dean of Students, with specific consequences that can include your permanent removal from the class. Disruptions include:
Being noisy when arriving late to class or leaving early. Carrying on private conversations while the professor is talking. Disrespectful language, tone, and mannerisms. Sleeping or attempting to sleep in class. Repeatedly asking unnecessary or irrelevant questions.
My wish is that you get the best learning value from the science classes that you are taking. With everyone abiding by the expectations in this letter, your science classes at Valencia should be the next step in achieving your academic dreams.
Dr. Robert GessnerWest Campus Dean of ScienceValencia College
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Valencia CollegeMicrobiology I–MCB 2010c Schedule
INSTRUCTOR
CRN 11048
Professor Nalini Odapalli
From 08/27/18 to 12/16/18 Mixed modeOFFICE HOURS In office HSB 117
Monday 8:30-11:30Tuesday 11:15- 2:00Wednesday 8:15- 8:30Thursday 8:15- 8:30Friday(phone) 10:00-5:00
If I am not in the office, please text me. Usually answering student questions after class so there might be a little delay to reach my office.By email/text: Sun- 10:00 AM -5:00 PM
CLASS TIME AND LOCATION
Lecture part will be covered through videos, notes, activity sheets, PowerPoints on the Canvas. In class we will be working on activity sheets and revision for tests. This will help you learn at your pace at home and master the concept in class through activities. Wednesday 8:30A- 11:15P; WC-AHS ROOM: 320
IMPORTANT DATES
September 3 Monday (Labor Day); No classesOctober 11 Thursday (College Night); No classesNovember 21 - 25 Wednesday through Sunday (Thanksgiving Holiday) No classesAugust 27- Monday Semester beginsSep 4- Drop/Refund Deadline (11:59 PM)Sep 5- Sep 14- No Show reporting by facultyNov 9- Withdrawal Deadline - "W" Grade (11:59 PM)Dec 9- Faculty Final Date to Enter Withdrawal and Term EndsDec 17- at 9 AM Grades dueDec 18- Final Grades Viewable in ATLASLecture Test dates will be posted on Canvas before one weekLecture test either will be in lecture class room or lab roomTest 1 in Chapters 1, 3, 4, 5Test 2 in Chapters - 9, 10, 11, 12Test 3 in Chapters 13, 14, 15, 16Test 4 in Chapters 17, 18, 19, 20Test 5 in Chapters 21, 22, 23Lab Midterm Oct 17 in lab onlyLab Final at Dec 5 in lab only
December 10 – 16 Valencia final exams week16
For CRN 11048: Dec 12, 2018 Wednesday Final Lec testTime 7:00AM- 09:30 AM in AHS 320; Caution: Test starts earlier than your class time.Note: The final tests’ date will be confirmed later
Microbiology MCB 2010C – FALL LAB AGENDA 2018
MICROBIOLOGY LAB WEEK 1 AGENDA(Aug 29th)
A. Review Lab Rules, Regulations and Safety (Students need to read pp. 1-6 in the lab manual)- A “scavenger hunt” may be used to locate and understand the need for
important safety and equipment features in the microbiology laboratory:- Security telephone (dial 1000 on west campus for security)- Disinfection solution for lab benches- Antiseptic had washing solution- Fire extinguisher- Emergency Shower an Eyewash station, Fire Blanket- MSDS folder- Biohazardous waste containers- Biohazardous sharps containers- Broken glassware container- “Kill Cart” (place for used unlabeled used test tubes)- Incubators (why are they set to 25°C and 37°C?)- Refrigerated cold room- Biological Safety Cabinet- Vortex mixers- Microscopes- First Aid Kit; Chemical Spill Kit- Inoculating loops, flint strikers, plastic pipettes, microscope lens
cleaner and lens paper, ethanol, distilled water, sterile swabs, test tube racks
- Autoclave (escort students to see in lab prep area)- Fire Alarm (near building exit doors)- Escape routes- Gas shut off
- Students need to be reminded that all lab seats need to go back under the tables when they leave. (It can be dangerous when the lights are off, if the seats are left in the aisles)
- Students also need to be told that the lab needs to look as nice when they leave as it did when they came in. They need to clean up after themselves!! (Tell them it’s the maid’s day off and that if they leave a mess the tests get harder
LOL)
B. Exercise 1-1 Glo-Germ™ Hand Wash Educational System (optional)Follow lab directions.1 bottle Glo-Germ™ lotion per classUV light chamber
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C. Exercise 2-1 Ubiquity of MicrobesFollow the lab directions; explain the proper way to label a Petri plate.7 lab groups per class; materials needed per student group of 4:
8 Nutrient Agar plates1 sterile cotton swab
Incubate plates at the designated temperatures.
D. Exercise 3-1 – Introduction to the Light MicroscopeStudents need to refresh their memory about the parts of the microscope,
as well as the proper use, care and storage of the microscopes. We have letter ”e” and three thread slides if you choose to start the students off with something
simple to view.
E. Exercise 3-3- Examination of Eukaryotic Microbes
Instructor can choose to have students look at organisms from the following slide trays:
1. Oscillatoria (cyanobacterium)2. Saccharomyces cerevisiae (yeast)3. Aspergillus4. Rhizopus (filamentous fungus)5. Penicillium6. Euglena7. Paramecium caudatum8. Amoeba9. Giardia lamblia10. Spirogyra11. Diatoms12. Volvox13. Dog tapeworm14. Human louse15. Plasmodium on human RBC smear
Please, make sure students clean and store their microscopes properly (this can be a part of any lab technique grade, if you assign oneJ )There are no VC classes on Monday, Sept 3rd due to Labor Day holiday!
MICROBIOLOGY LAB WEEK 2 AGENDA(Sept 5th)
A. Look at and discuss the results from Exercise 2-1 from last week.
B. Have students read Exercise 1-2 about Nutrient Agar and Broth Preparation and pp. 59-66 about Colony Morphology.
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Aseptic Technique and Continuation of Microscope Practice
NOTES:- Please, put all culture tube racks in a green tray when incubating, this helps to prevent
spillage of media in the incubator which has attracted ants. If you don’t have an assigned incubator shelf, ask Shivani and she will help you find one.
- Used culture tubes go in test tube racks on the kill cart, please have students fill the racks already there, before adding new ones. The students are not to leave their partially filled or empty test tube racks in that area, unless all of the racks are full of test tubes. All labels must be removed from culture tubes, using alcohol, before the tubes can be placed on the kill cart.
- Used agar plates go in the biohazard containers. If they are full, please make new biohazard containers. (Extra biohazard containers are in the lab prep
room next to the autoclaves.)
C. Exercise 1-4 Common Aseptic Transfers and Inoculation MethodsHave students read page 19 about aseptic technique.Each group of 4 students needs:
4 Nutrient Broth tubes4 Nutrient Agar slants
1 nutrient broth culture of Micrococcus luteus1 nutrient agar slant of Micrococcus luteus1 nutrient agar slant of Escherichia coli1 nutrient agar plate of Micrococcus luteus
7 Lab groups per class; have each lab group follow the procedure on p. 40:1. Have different students aseptically transfer each of the microbes to the
appropriate media so that all get the opportunity to do 2 aseptic transfers.2. Incubate at 37oC for 24-48 hours.
D. Exercise 1-5 Streak Plate Methods of IsolationEach group of 4 students needs:
5 Trypticase Soy Agar plates1 nutrient broth culture of Staphylococcus epidermidis 1 nutrient broth culture of Serratia marcescens1 sterile swab for environmental culture1 sterile water tube 1 sterile test tube
7 Lab groups per class; have each lab group follow this procedure:1. Transfer a few drops, using a sterile transfer pipette of S. marcescens and a few
drops, using a different sterile transfer pipette of S. epidermidis to a sterile test tube and mix well. Each student needs to perform an isolation streak on NA or TSA, using an inoculating loop to aseptically streak the mixed broth culture that they created for isolation.
2. On the 5th NA or TSA plate have the students, as a group, moisten a sterile swab with sterile water and obtain a sample from some environmental source, inoculate a NA or TSA plate with the zigzag inoculation method described in the lab manual.
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E. Exercise 2-11 STEAM STERILIZATION (optional)
You may want to bring your students on a “field trip” into the biology prep lab to show your students the materials used to make media and to show them the autoclave and how it works.
F. Exercise 3-3 EXAMINATION OF EUKARYOTIC MICROBESHave students review exercise 3-1 on the use of the light microscope.Students can work individually or in groups to observe, draw and be able to recognize the
various microscopic organisms found in the slide trays on the instructor’s side table. Please, make sure students clean and store their microscopes properly,
particularly if using oil!!(this can be a part of any lab technique grade, if you assign one J )
Instructor can choose to have students look at organisms from the following slide trays:
1. Oscillatoria (cyanobacterium)2. Saccharomyces cerevisiae (yeast)3. Aspergillus 4. Rhizopus (filamentous fungus) 5. Penicillium6. Euglena7. Paramecium caudatum8. Amoeba9. Giardia lamblia10. Spirogyra11. Volvox12. Diatoms13. Dog tapeworm14. Human louse15. Plasmodium on human RBC smear
- Make sure microscopes are cleaned and checked before students check out.- Students need to clean up the lab before they leave.
MICROBIOLOGY LAB WEEK 3 AGENDA(Sept 12th)
A. Review Results of last week’s exercises 1-4 and 1-5
- Used test tubes go in test tube racks on the “kill cart”, please have students fill the racks already there. The students are not to leave their partially filled or empty test tube racks in that area, unless all of the racks are full of test tubes. All test tube labels must be removed
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with alcohol before tubes go to the kill cart.- Used agar plates go in the biohazard containers. If they are full, please make
new containers (new biohazard buckets are in the biology prep room, near the autoclaves.
B. Students need to read pp. 79 and 83 about growth patterns on slants and broth. Students also should read pp. 173-176.
C. STAINING LAB IExercise 3-4 SIMPLE STAINS
Students can work in lab groups of 4 (each table), so that all of the organisms are observed by the group of 4 students. Have each pair of students also choose a different stain from the other partners and have all students share their results with the group.
Materials needed for each group:Glass slidesSoft soap to clean slidesLens paper, lens cleaner24- 48 hr nutrient agar slant cultures of Micrococcus luteus24- 48 hr nutrient agar slant cultures of Bacillus cereus24- 48 hr nutrient agar slant cultures of Aquaspirillum itersonii(optional: have students use sterile tooth picks to scrape cheek cells and see their cells, nuclei and bacteria from their
mouth- carbolfuchsin works well for this)Methylene blueCrystal violetCarbolfuchsinSafranin
- Students are to clean off their simple stain slides before replacing them in the acid alcohol wash (glass specimen dish).
EXERCISE 3-5 NEGATIVE STAINS
Have students work in their lab groups of 4 so that each student gets to see each organism.
Materials needed for each group:Glass slides24 hr agar slant cultures of Micrococcus luteus24 hr agar slant cultures of Bacillus cereus 24 hr agar slant cultures of Aquaspirillum itersoniiNigrosin (or India Ink)
IMPORTANT NOTE: The bacterial spores on the negative staining slides are not killed in this procedure!! Slides
needed to perform a negative stain are NOT to go in the acidalcohol wash basin; THESE SLIDES ARE
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TO GO IN THE BIOHAZARDSHARPS CONTAINER!! (No other slides, paper towels, or gloves in the biohazard sharps box, pleaseJ)
Exercise 3-10 – WET MOUNT PREPARATIONS
Materials needed: Clean slides Coverslips Pipette Pond water sample from Lake Pamela
A fresh pond water sample from Lake Pamela will be provided for students to learn about wet mount preparations.Directions are found on page.
- Make sure microscopes are cleaned and checked before students check out.- Students need to clean up the lab before they leave.
MICROBIOLOGY LAB WEEK 4 AGENDA(Sept 19th)
STAINING I
A. Have students review steps to bacterial smear prep and discuss differential stains.B. Exercise 3-6 GRAM STAIN
Materials needed for each group of 4:Microscope slides and soft soap to clean themMicroscopes lens cleaner and paperSterile toothpicksGram staining kits: Crystal violet, Gram’s iodine, 95%
Ethanol, Safranin24 hour nutrient agar slant cultures of E. coli 24 hour nutrient agar slant cultures of Micrococcus luteus
Follow the directions in the lab procedure including the option to have the students also make a smear of the plaque between their teeth and gums and gram stain this materialJ. (This is a good time, if you like to assess student skills, to observe each student’s skill of using a microscope up through the oil immersion lens)
C. Exercise 3-7 ACID-FAST STAIN
We have prepared slides of Mycobacterium smegmatis for the students to observe Ziehl-Neelsen acid fast staining.
D. Exercise 3-9 ENDOSPORES We have a prepared slide of Clostridium botulinum for the students to
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observe Schaeffer-Fulton spore staining and position of spores.
E. We will inoculate the following media so that it can be analyzed during next week’s lab. Have students read pp. 227-228. Follow the procedure for each exercise and incubate plates as directed in the exercises:
Exercise 4-1 PHENYLETHYL ALCOHOL AGAREach group of 4 students needs:
1 PEA plate1 NA plate1 nutrient broth culture of Escherichia coli1 nutrient broth culture of Enterococcus faecalis1 nutrient broth culture of Staphylococcus aureus
Exercise 4-2 COLUMBIA CNA with 5% SHEEP BLOOD AGARBesides the Candle Jar, Each group of 4 students needs:
1 Columbia CNA with 5% Sheep Blood Agar1 NA plate1 nutrient broth tube of Enterococcus faecalis1 nutrient broth tube of Escherichia coli1 nutrient broth tube of Staphylococcus aureus1 blood plate of Streptococcus mitis
Exercise 4-3 MANNITOL SALT AGAREach group of 4 students needs:
1 MSA plate1 NA plate1 nutrient broth culture of Staphylococcus aureus1 nutrient broth culture of Staphylococcus epidermidis 1 nutrient broth culture of Escherichia coli
Exercise 4-4 MacCONKEY AGAREach group of 4 students needs:
1 Mac plate1 NA plate1 nutrient broth culture of Escherichia coli1 nutrient broth culture of Salmonella typhimurium1 nutrient broth culture of Enterococcus faecalis
Exercise 4-5 EOSIN METHYLENE BLUE AGAREach group of 4 students needs:
1 EMB plate1 NA plate1 nutrient broth culture of Enterobacter aerogenes1 nutrient broth culture of Escherichia coli1 nutrient broth culture of Enterococcus faecalis1 nutrient broth culture of Salmonella typhimurium
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Exercise 4-6 HEKTOEN ENTERIC AGAREach group of 4 students needs:
1 HE Agar plate1 NA plate1 nutrient broth culture of Enterococcus faecalis1 nutrient broth culture of Escherichia coli1 nutrient broth culture of Salmonella typhimurium1 nutrient broth culture of Shigella flexneri
MICROBIOLOGY WEEK 5 AGENDA
(Sept 26th)
A. SELECTIVE and DIFFERENTIAL MEDIA
- Have students read pp. 95- Have students analyze and discuss exercises 4-1, 4-2, 4-3, 4-4, 4-5 and 4-6 that were
inoculated last week.- Dispose of Petri dishes in biohazard containers
B. Inoculate media for week 6:- Students to work in lab groups of 4; have them follow the directions for inoculation and
incubation requirements.
Exercise 2-6 FLUID THIOGLYCOLLATE MEDIUMEach group of 4 students needs:
4 FTM tubes with resazurin dye1 nutrient broth culture of Pseudomonas fluorescens1 nutrient broth culture of Clostridium sporogenes1 nutrient broth culture of Escherichia coli
Exercise 2-8 THE EFFECT OF TEMPERATURE ON MICROBIAL GROWTH
We will use incubators that are set at 5°C, 25°C, 37°C, and 60°CEach group of 4 students will need:
4 nutrient broth tubes (Have each student lab group choose one of the organisms to test below in each
of the different temperatures)2 TSA plates (For 25°C and 37°C Serratia marcescens
experiment) 1 nutrient broth culture of Escherichia coli1 nutrient broth culture of Serratia marcescens1 nutrient broth culture of Bacillus stearothermophilus1 nutrient broth culture of Pseudomonas fluorescens
Exercise 5-10 STARCH HYDROLYSISEach group of students will need:
1 Starch Agar Plate1 Nutrient Broth culture of Bacillus cereus
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1 Nutrient Broth culture of Escherichia coli
Exercise 5-11 DNA HYDROLYSISEach group of students will need:
1 DNA Agar Plate1 Nutrient Broth culture of Serratia marcescens1 Nutrient Broth culture of Escherichia coli
*Incubate cultures at 37oC to avoid pigmentation in Serratia marcescens.
Exercise 5-12 LIPID HYDROLYSISEach group of students will need:
1 Tributyrin Agar Plate1 Nutrient Broth culture of Serratia marcescens1 Nutrient Broth culture of Escherichia coli
*Incubate cultures at 37oC to avoid pigmentation in Serratia marcescens.
Exercise 5-13 CASEIN HYDROLYSISEach group of students will need:
1 Milk Agar Plate1 Nutrient Broth culture of Pseudomanas aeruginosa 1
Nutrient Broth culture of Escherichia coli
EXERCISE 5-16 BILE ESCULIN TESTEach group of students will need:
1 Bile Esculin plate1 Nutrient broth culture of Escherichia coli1 Nutrient broth culture of Enterococcus faecalis
MICROBIOLOGY WEEK 6 AGENDA (Oct 3rd)
A. PHYSICAL CONTROL of MICROBIAL GROWTH
- Students need to read pp. 77, 207 and 237- Observe and analyze the Exercises 2-6, and 2-8 that were inoculated last week.
B. MICROBIAL NUTRITION
- Observe and analyze the Exercises 5-10, 5-11, 5-12, 5-13 and 5-16 that were inoculated last week.
Students will need Lugol’s Iodine reagent.
C. INTRODUCTION TO BIOCHEMICAL TEST MEDIA
EXERCISE 5-4 CATALASE TEST
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Each group of students will need:1 NAS culture of Staphylococcus aureus1 NAS culture of Enterococcus faecalisHydrogen peroxide (3%) reagent1 glass slide
EXERCISE 5-5 OXIDASE TESTEach group of students will need:
1 NAS culture of Escherichia coli1 NAS culture of Pseudomonas aeruginosa (substituted for
Neisseria)Oxidase test reagent
EXERCISE 5-6 NITRATE REDUCTION TESTEach group of students will need:
4 nitrate broth tubes w/Durham tubes1 NAS culture of Escherichia coli1 NAS culture of Pseudomonas aeruginosa1 NAS culture of Micrococcus luteusNitrate reagents A and BZinc powder
EXERCISE 5-9 PHENYLALANINE DEAMINASE TESTEach group of students will need:
2 Phenylalanine agar slants1 NAS culture of Escherichia coli1 NAS culture of Morganella morganii12% Ferric chloride reagent solution
EXERCISE 5-19 KLIGLER IRON AGAREach student group will need:
5 KIA slants1 NAS culture of Pseudomonas aeruginosa1 NAS culture of Escherichia coli1 NAS culture of Morganella morganii1 NAS culture of Salmonella typhuimurium
EXERCISE 5-23 COAGULASE TESTEach student group will need:
2 sterile Rabbit plasma tubes1 NAS culture of Staphylococcus aureus (BSL-2)1 NAS culture of Staphylococcus epidermidis
Oct 10th There will be a lecture session in the lab room.
MICROBIOLOGY LAB WEEK 7 AGENDA
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(Oct 17th)
A. MIDTERM LAB EXAMS
B. Analyze the following test results from last week’s labs 5-6, 5-9, 5-10, 5-18. 5-19, 5-20 and 5-21. (You have the option to read these labs today or during the next lab period)
MICROBIOLOGY WEEK 8 AGENDA(Oct 24th)
A. CULTURE and SENSITIVITY (C&S) LAB
During this period students will inoculate various biochemical tests to identify an enteric bacterium, and will inoculate Mueller Hinton Agar for antibiotic sensitivity testing. After the incubation is complete, each of the biochemical tests can be discussed with the CD that compliments the lab manual to show positive and negative controls. Students can also read page 545 and instructors can have students look at Exercises 9-4 and 9-5 to see how miniaturized, rapid multi-test systems are used today in modern microbiology labs (we have an Enterotube II and an API 20E strip for the students to observe). Next week the students can complete this lab when they are left on their own to determine what
unknown they were given, using the flow charts in Exercise 9-1. Students will also determine how antibiotics are chosen that would be effective in treating a patient with an E. coli or Staph. aureus infection as when a Culture and Sensitivity (C & S) is performed.
(*** LAB TECHNICIANS*** please do a sample run of all microbes on each of the assigned media one week in advance of this lab to ensure microbial viability; for
those microbes which show incorrect media results, new microbes need to be ordered so that the results are correct for the students)
1) Exercise 9-1 IDENTIFICATION OF ENTEROBACTERIACEAEEach lab group of 4 students will need:
1 of the following unknowns: Escherichia coliCitrobacter freundiiEnterobacter aerogenesProteus mirabilisMorganella morganii
Each lab group of 4 students will need:1 Simmons Citrate Agar1 Phenol Red Glucose Broth1 Phenol Red Mannitol Broth1 Phenol Red Lactose Broth1 Phenol Red Xylose Broth1 SIM Tube (Sulfide Indole Motility Media)1 Lysine Decarboxylase Broth
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1 Ornithine Decarboxylase Broth1 MRVP Broth1 Urea Broth1 Nutrient Gelatin Tube
2) ENTEROTUBES - Have students read pp. 573-574 on the API 20 E system and Exercise 9-5 for the EnteroPluri test.
3) Exercise 7-2 ANTIMICROBIAL SUSCEPTIBILITY TEST(KIRBY BAUER METHOD)
Each group of 4 students will need:1 nutrient broth culture of Escherichia coli1 nutrient broth culture of Staphylococcus aureus2 Mueller Hinton agar plates1 Set of antibiotic discs: ciprofloxacin, trimethoprim,
penicillin and chloramphenicol. (2 discs of each per station)1 Small beaker of alcohol
Forceps
MICROBIOLOGY WEEK 9 AGENDA(Oct 31st)
A. Analyze the results of last week’s UNKNOWN LAB (Exercise 9-1). - When using reagents which come in individual mini-reagent droppers,
please conserve by having lab groups share the reagents in one mini-reagent dropper before breaking open another one. With so many classes this will help us ensure adequate supplies and keep our costs to a practical level. Thank you J - Remember, no empty test tube racks go in the biohazard kill area.
Please have students store empty test tube racks where they belong. Discard Petri plates in biohazard container.
B. Analyze the results of last week’s KIRBY BAUER ANTIBIOTIC SENSITIVITY LAB (Exercise 7-2)
- Students will work in groups of 4 and need rulers.
MICROBIOLOGY WEEK 10 AGENDA(Nov 7th)
A. Exercise 2-12 and 8-2 THE LETHAL EFFECT of ULTRAVIOLET LIGHT on MICROBIAL GROWTH- students work in lab groups of 4, each group needs:
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- 1 24 hour nutrient broth culture of S. marcescens- 1 7 day nutrient broth culture of Bacillus cereus- 1 7 day saline broth of Aspergillus niger- 1 24 hour nutrient broth culture of Escherichia coli- 5 TSA plates (inoculate each plate in quadrants, each
quadrant containing one of the four microbes listed above; expose 1 plate to no UV radiation to act as a control; expose a different plate for 1 minute with the lid off; expose the third plate for 3 minutes with the lid off; expose the fourth plate for 5 minutes with the lid off and expose the fifth plate for 5 minutes but with the lid left on.)
- Ultraviolet radiation source (254 nm.)
B. Exercise 8-2 BACTERIAL TRANSFORMATION: the pGLO SYSTEM
- students work in lab groups of 4- Follow instructions on pp. 509 - 512
Each group needs one pGLO kit setup
MICROBIOLOGY WEEK 11 AGENDA(Nov 14th)
A. Read the results from last week’s lab exercises 2-12 (8-2) and 8-3.
B. Have students read pp. 391
C. Exercise 8-6 ELISA TEST Handouts will be provided to make copies for your students.
Each group needs an ELISA test setup
D. Exercise 7-4 EPIDEMIOLOGY SIMULATIONEach class will need:
1 Nutrient broth culture of Serratia marcescens
Each STUDENT needs:- 1 Petri dish containing one candy corn- 1 Sterile Saline Tube- Lab gloves- 1 TSA plate
There are no labs scheduled for Nov 19th -24th due to the Thanksgiving holiday!!
MICROBIOLOGY WEEK 12 AGENDA(Nov 28th)
A) Review results of last week’s lab exercise.
B) Exercise 6-5 DIFFERENTIAL BLOOD CELL COUNT29
Students will need commercially prepared human blood smearslides (see if we can get some slides with abnormal white blood cell counts)
MICROBIOLOGY WEEK 13 AGENDA(Dec 5th)
FINAL LAB EXAM – Study week 7 to week 12 labs
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