FURTHER PRODUCTS FROM PRESSURE BIOSCIENCES INC.:

DUAL HEAD AIR COMPRESSOR

RELEVANT PUBLICATIONS BAROCYCLER

PCT-HD: TISSUE TO PEPTIDES IN 4 HOURS

PCT-HD: HOMOGENIZATION, EXTRACTION AND FAST DIGESTION OF SMALL TISSUE SAMPLES FOR PROTEOMIC APPLICATIONS

HUB 440 OR HUB 880 MODEL

For more information: www.il-biosystems.com

• Quiet operation• Portable, fits easily under lab bench• For use with 2320EXT and NEP2320-ENH• Suitable for Pressure Cycling applications up to 45kpsi

High pressure generator for controlled, hydrostatic pressure in parts of a second up to 4000 bar (58 KPSI) or 6200 bar (90 KPSI)• Tissues to Peptides in 4 hours for analysis

• For Use With Minute/Valuable tissue Biopsy Samples and Low Number Cells

• Use Fresh, Frozen or FFPE Tissues• Rapid 1 Hour Digestion of Proteins by PCT

• Compatible with Trypsin, Lys-C, PNGase F, Chymotrypsin etc.

• Process Up to 16 Samples Simultaneously• Inert, Single-Use MicroPestles and MicroTubes

Pressure Cycling Technology (PCT) is a unique, patented technology platform based on repeated cycles of hydro- static pressure between ambient and ultra-high levels. These rapid changes in pressure are used to control biomolecular interactions for applications such as acce- lerated proteolytic digestion, and improved extraction of cellular components, including proteins and lipids. The PCT platform allows for a high degree of safety, speed, reproducibility, and convenience in modern sample preparation protocols.

The PCT-HD Sample Preparation System is a proven PCT-based workflow for processing small tissue samples for proteomic and other applications. PCT-HD com-bines efficient, hands-off tissue homogenization and protein extraction with pressure-enhanced digestion, for rapid, efficient and reproducible generation of peptides for MS analysis. This unique workflow combines two of PBI’s innovative sample preparation tools: the Barocycler 2320EXT and our patent-pending PCT MicroPestles.

PCT-HD and the Barocycler 2320EXT can provide unpre- cedented speed and reproducibility for biomarker dis- covery, yielding peptides ready for clean-up and MS within 2-4 hours from the start of tissue processing.

The Barocycler 2320EXT is a compact, bench top instru-ment that can process up to 16 samples simultaneously using PCT MicroTubes. Features that come standard with the 2320EXT include: data input and output options to accommodate validation and quality con-trol; computer-operated control with touch screen programming and automatic data logging; and the ability to control multiple pressure cycling parame-ters, such as the rate of pressure increase/decrease, both the high and low pressure levels, and the shape of the pressure profile (e.g., sine wave, square wave, and others). In addition, the 2320EXT is availa-ble with a choice of two different methods for tem-perature control (an external circulating waterbath for heating and cooling, or a built-in electric heater).

www.pressurebiosciences.com/publication• Proteomics• Lipidomics• Food• FFPE Tissue• Forensics• Pressure-Enhanced Enzymes• Mitochondria

Microbiology Cell Biology Biotechnology Process Control

I&L Biosystems GmbHKönigswinterer Straße 409

53639 Königswinter | GermanyTel.: +49 2223 9192-0

Fax: +49 2223 9192-48 info@il-biosystems.de

www.il-biosystems.com

Streamlined hands-free sample preparation using Pressure Cycling and PCT μPestle sample containers for tissue biopsy profiling by SWATH-MS. Vera Gross1; Keith Ashman2; Peter Hains3; Alexander Lazarev*,1; Chris Hodgkins2; Phil Robinson3 INT’L HUPO 2016

Barocycler® 2320EXT For the PCT-HD Sample Preparation System

• IQ/OQ documents

• T-Control: Electric Heat ot Water Jacket

• Computer-operated control with Touch Screen Interface

• Pressure Range: up to - 45,000psi (310 MPa)

• Choice of temperature control - heating/cooling or heating only

• Max Temperature: 95°C

• Min Temperature: 4°C with circulating waterbath option, ambient with electric heating

• Insulated closure for easier use at high temperatures

• Chamber capacity: up to 16 samples in MicroTubes

• Easily accessible USB port on front panel

• Pressure medium: Distilled Waterwww.il-biosystems.com

Germany – Netherlands – France – United Kingdom – Nordic

• Touch Screen Programming• Maximum Pressure: 45,000psi• Maximum Hold Time: Indefinite• Maximum Cycle Number: Indefinite• On Board Temperature Control Option

• Real time display and log of operating pressures and temperature • Data can be saved and exported in CSV file format • Standard protocols can be set and loaded

• MicroTube Adapter Cartridges• Stainless Steel• Cartridges are lettered and numbered for sample identification• Allow for up to 16 samples to be processed simultaneously

CAPS VARY IN SIZE:

• 50uL• 100uL• 150uL• MicroPestle (30uL)

MICRO TUBE VOLUMES:

• 1uL – 150uL• Tubes are the same volume• Outside of tubes is writable• Constructed from low binding,

non-wetting Teflon like material

USES:

• PCT-HD & Biopsy tissue processing• Process up to 3mg of solid tissue• Process as few as 5 x 104 cells

MICROTUBES AND PULSE TUBESNEW PROTEOMIC SAMPLE PREPARATION

The PCT MicroPestle fits into the MicroTube acting as a disposable tissue homogenizer.Tissue samples are homogenized under identical pressure conditions ensuring highly reproducible preparation of tissue biopsy samples.

CARDIAC MUSCLE LUNG TISSUE LIVER TISSUE

• MicroTube Adapter Cartridges• Stainless Steel• Cartridges are lettered and numbered for sample identification• Allow for up to 16 samples to be processed simultaneously

The PCT MicroPestle fits into the MicroTube acting as a disposable tissue homogenizer.Tissue samples are homogenized under identical pressure conditions ensuring highly reproducible preparation of tissue biopsy samples.