Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011)

Microbial for ACP ManagementMicrobial for ACP Management

Cooperative Work PlanCooperative Work Plan

betweenbetween

NCSU/CIPM & FAAS/ABRRINCSU/CIPM & FAAS/ABRRI

(2010-2011)(2010-2011)

1.1. IntroductionIntroduction

2.2. Work planWork plan

3.3. FoundationFoundation

Presentation ContentPresentation Content

IntroductionIntroduction Damage of Huanglongbing (HLB)Damage of Huanglongbing (HLB)

Citrus HuanglongbingCitrus Huanglongbing

Damage of Huanglongbing Damage of Huanglongbing

IntroductionIntroduction Vector of HuanglongbingVector of Huanglongbing

Transmitting HLBTransmitting HLB

Diaphorina citriDiaphorina citri (ACP)(ACP)

IntroductionIntroduction

Control of ACPControl of ACP

Controlling ACP Controlling ACP

is a main and is a main and

effective effective

approach to approach to

manage HLBmanage HLB

IntroductionIntroductionAdvancement of ACP ControlAdvancement of ACP Control

Chemical controlChemical control

ConventionalConventional approachapproach

Usually undesirableUsually undesirable

Many side effectsMany side effects

IntroductionIntroductionAdvancement of ACP ControlAdvancement of ACP Control

Biological controlBiological control

Environmentally Environmentally friendlyfriendly

Few biological Few biological agents studiedagents studied

No biological No biological agents used in agents used in practice of ACP practice of ACP controlcontrol

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IntroductionIntroductionPotential of microbial for bio-control of ACPPotential of microbial for bio-control of ACP

1.1. Microbial are resources of biological agents Microbial are resources of biological agents against pest insectsagainst pest insects

Biological pesticidesBiological pesticides Gene resource for GMPGene resource for GMP

IntroductionIntroductionPotential of microbial for bio-control of ACPPotential of microbial for bio-control of ACP

2. Reported microbial against ACP2. Reported microbial against ACP

Cephalosphorium Cephalosphorium lecaniilecanii Zimm Zimm

Isaria fumosorosea Isaria fumosorosea WizeWize

Work planWork planObjectiveObjective

Find new microbial for ACP Find new microbial for ACP managementmanagement

Work planWork planContentContent

1.1. Occurrence of ACP microbial parasites in field Occurrence of ACP microbial parasites in field

2.2. Isolation and reconfirmation of ACP microbial Isolation and reconfirmation of ACP microbial parasites parasites

3.3. Identification of ACP microbial parasitesIdentification of ACP microbial parasites

4.4. Biology of ACP microbial parasitesBiology of ACP microbial parasites

5.5. Production technology of ACP microbial Production technology of ACP microbial parasitesparasites

6.6. Evaluation of the efficiency of ACP microbial Evaluation of the efficiency of ACP microbial parasitesparasites

Work planWork plan1.1. Occurrence of ACP microbial parasites in fieldOccurrence of ACP microbial parasites in field

1.1 Population dynamic of 1.1 Population dynamic of ACPACP

1.2 Occurrence of ACP 1.2 Occurrence of ACP parasitesparasites

1.3 Contribute of ACP 1.3 Contribute of ACP parasites to regulating parasites to regulating ACP populationACP population

1.4 Collection of ACP 1.4 Collection of ACP parasites from field parasites from field

Timeline: 2010/04-2011/04Timeline: 2010/04-2011/04

Work planWork plan2. Isolation and reconfirmation of ACP microbial parasites2. Isolation and reconfirmation of ACP microbial parasites

2.1 Rearing ACP for bioassay2.1 Rearing ACP for bioassay

2.2 Isolating and reconfirming 2.2 Isolating and reconfirming microbial pathogenic against ACPmicrobial pathogenic against ACP

2.2.1 Microbial from infected ACP2.2.1 Microbial from infected ACP

2.2.2 Microbial inhabiting on plant 2.2.2 Microbial inhabiting on plant surface surface

2.2.3 Microbial endophyte inner plant2.2.3 Microbial endophyte inner plant

2.2.4 Microbial from soil2.2.4 Microbial from soil


Work planWork plan3. Identification of ACP microbial parasites3. Identification of ACP microbial parasites

3.1 Fungi pathogenic against ACP3.1 Fungi pathogenic against ACP

3.2 Bacteria pathogenic against ACP3.2 Bacteria pathogenic against ACP

3.3 Virus pathogenic against ACP3.3 Virus pathogenic against ACP

(Send to taxonomist for identification)(Send to taxonomist for identification)


Work planWork plan4. Biology of ACP microbial parasites4. Biology of ACP microbial parasites

4.1 Climatic condition for microbial growth4.1 Climatic condition for microbial growth

4.2 Nutrition condition for microbial growth4.2 Nutrition condition for microbial growth

4.3 Effects of climatic condition on 4.3 Effects of climatic condition on microbial pathogenicitymicrobial pathogenicity

4.4 Effects of nutrition medium on 4.4 Effects of nutrition medium on microbial pathogenicitymicrobial pathogenicity


Work planWork plan5. Production technology of ACP microbial 5. Production technology of ACP microbial

parasitesparasites

5.1 Fermentation condition 5.1 Fermentation condition

Medium formula, temperature, Medium formula, temperature, inoculum, rotation rate, etc. inoculum, rotation rate, etc.

5.2 Formation5.2 Formation


Work planWork plan6. Evaluation of the efficiency of ACP 6. Evaluation of the efficiency of ACP

microbial parasitesmicrobial parasites

6.1 Greenhouse experiments6.1 Greenhouse experiments

6.2 Field experiments6.2 Field experiments


Work planWork planDeliverable resultsDeliverable results

1.1. Report of population dynamic of ACP Report of population dynamic of ACP and the natural occurrence of its and the natural occurrence of its microbial parasitesmicrobial parasites

2.2. Isolation of 2-3 microbial strains against Isolation of 2-3 microbial strains against ACPACP

Work planWork planBudget for this yearBudget for this year

Personel (salary etc): 7400 USDPersonel (salary etc): 7400 USD

Materials: 3000 USDMaterials: 3000 USD

Travels: 4000 USDTravels: 4000 USD

Eletricity energy: 1600 USDEletricity energy: 1600 USD

Collaborator: 3000 USDCollaborator: 3000 USD

Overhead: 1000 USDOverhead: 1000 USD

Sum: 20000 USDSum: 20000 USD

Personal:Personal:

We need to pay our students and scientists working on We need to pay our students and scientists working on the projectthe project

Tow graduate students, 150 USD/month, 10 month for Tow graduate students, 150 USD/month, 10 month for work, total 3000 USDwork, total 3000 USD

tow months of a senior scientist, 1000 USD/month, total tow months of a senior scientist, 1000 USD/month, total 2000 USD2000 USD

One full time research assistant, 200 USD/month, 12 One full time research assistant, 200 USD/month, 12 month for work, total 2400 USDmonth for work, total 2400 USD

Sum 7400 USDSum 7400 USD

Work planWork planBudget Narrative 1Budget Narrative 1

Materials: Materials:

Purchasing citrus seedlings and culturing pots to rear Purchasing citrus seedlings and culturing pots to rear Asian Citrus Psyllid, 1500 USDAsian Citrus Psyllid, 1500 USD

Purchasing medium, Petri discs for culturing microbials, Purchasing medium, Petri discs for culturing microbials, 1500 USD1500 USD


Travels:Travels:

Travel to citrus orchards in different regions for Travel to citrus orchards in different regions for investigation and sampling, investigation and sampling, 4000 USD4000 USD

Spending of eletricity energy: Spending of eletricity energy: 1600 USD1600 USD


Collaborator:Collaborator:

Payment for citrus farmer for their information and Payment for citrus farmer for their information and offering citrus orchard for experiment, 1000 USDoffering citrus orchard for experiment, 1000 USD

Identification of entomopathogens of Asian Citrus Identification of entomopathogens of Asian Citrus Psyllid, 2000 USDPsyllid, 2000 USD


Overhead:Overhead:

The fund for the institute to maintain the facilities on The fund for the institute to maintain the facilities on good condition for conducting experiments, good condition for conducting experiments, 1000 USD1000 USD


FoundationFoundation1.1. Long history of study on Huanglongbing and Long history of study on Huanglongbing and its vectorits vector

• Morphology of HLB PathogenMorphology of HLB Pathogen• Pathology and epidemiology of HLBPathology and epidemiology of HLB• Biology, ecology and control of ACPBiology, ecology and control of ACP

FoundationFoundation1.1. Long history of study on Huanglongbing and Long history of study on Huanglongbing and its vectorits vector

• Morphology of HLB PathogenMorphology of HLB Pathogen• Pathology and epidemiology of HLBPathology and epidemiology of HLB• Biology, ecology and control of ACPBiology, ecology and control of ACP

FoundationFoundation2.2. Profound research and utilization of microbialProfound research and utilization of microbial2.1 Collection center of microbial resource2.1 Collection center of microbial resource

>5000 soil samples >5000 soil samples from different from different regions over the regions over the worldworld

>10000 >10000 BacillusBacillus strainsstrains

FertilizerFertilizer

PesticidePesticideFeedstuffFeedstuff

ClarifierClarifier

PreservativePreservative

Bacillus

Bacillus

AAR&D R&D

CenterCenter

FoundationFoundation2.2. Profound research and utilization of microbialProfound research and utilization of microbial2.2 Utilization of microbial2.2 Utilization of microbial

FoundationFoundation2.2. Profound research and utilization of microbialProfound research and utilization of microbial2.3 Research projects2.3 Research projects2.3.1 Study on Bio-insecticide BtA2.3.1 Study on Bio-insecticide BtA

FoundationFoundation2.2. Profound research and utilization of microbialProfound research and utilization of microbial2.3 Research projects2.3 Research projects2.3.2 Study on Bio-bactericide strain ANTI-8098A 2.3.2 Study on Bio-bactericide strain ANTI-8098A against against Ralstonia solanacearum Ralstonia solanacearum of vegetablesof vegetables

FoundationFoundation

2.2. Profound research and utilization of microbialProfound research and utilization of microbial2.3 Research projects2.3 Research projects2.3.3 Study on Bio-preservative for fruit Long’an2.3.3 Study on Bio-preservative for fruit Long’an

Bio-preservative for fruit Long’anBio-preservative for fruit Long’an


3. Condition for research3. Condition for research


4. Research group4. Research group


4. Publications4. Publications

Thank you for your Thank you for your attention !attention !

Thank you for your Thank you for your attention !attention !

Wellcome to China !

Documents

Microbial for ACP Management Cooperative Work Plan between NCSU/CIPM & FAAS/ABRRI (2010-2011)