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DNA microarray Pavlo Tertychnyi 6 March 2017

microarray DNA - Kursused - Arvutiteaduse instituut · Presentation outline 1. What is DNA microarray 2. DNA microarray usage 3. How does it work 4. Analyzing of results

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DNAmicroarrayPavlo Tertychnyi6March2017

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

WhatisDNAmicroarray

WhatisDNAmicroarray

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Medicalfield

Helpresearcherslearnmoreaboutdiseases:1. Whatcausesdiseases2. Howtoidentifydiseases3. Howtotreatthem

Multiplesclerosis

Foodproduction

Foodproduction

Please,don’teatme…

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Howdoesitwork

Howdoesitwork

Howdoesitwork

Example

Presentationoutline

1. WhatisDNAmicroarray2. DNAmicroarrayusage3. Howdoesitwork4. Analyzingofresults

Analyzingofresults

Analyzingofresults

Analyzingofresults

Analyzingofresults

DNAmicroarraydatabases

Summary

1. Usedtoidentifyspecificgenesandtheactivityofgenes.2. Usedtodeterminehowourgenesareaffectedbyenvironmental

factors,drugsanddrugdosages.3. Wideusedinmedicinefield4. Identifyspecificcharacteristicsinhumanaswellasotherspecies.5. Foodproduction

Coolvisualization

https://www.youtube.com/watch?v=VNsThMNjKhM

Acknowledgement1. http://scme-

nm.org/index.php?option=com_docman&task=cat_view&gid=97&Itemid=225

2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4011503/pdf/nihms-563981.pdf

3. http://www.ub.edu/stat/docencia/bioinformatica/microarrays/ADM/slides/A_Tutorial_Review_of_Microarray_data_Analysis_17-06-08.pdf

4.http://www.ebi.ac.uk/arrayexpress/5.https://www.ncbi.nlm.nih.gov/geo/6.http://www.hhmi.org/biointeractive/how-analyze-dna-microarray-data

Homework

1.WhatisDNAmicroarray?

a)Specificdatatypeb)Microchipc)SubsequenceofaDNAd)Giftofthegods

Homework

2.Describeatleast3applicationofDNAmicroarray(Imentionedtwoapplicationsduringthepresentation,souseyourimaginationorjustgoogletofindmore).3.DescribetheprocessofpreparationofamaterialbeforepouringitonaDNAmicroarray.

Homework

4.Jointhenextstatements:

a)Therearegenesofahealthycellmainly.b)Therearegenesofasickcellmainly.c)Therearegenesofbothhealthyandsickcells.

1)RedcolorofDNAmicroarrayunit.2)GreencolorofDNAmicroarrayunit.3)YellowcolorofDNAmicroarrayunit.

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Thanks!