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8/20/2019 Microalgal species in the modern times
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Botryococcus brauniiIsochrysis sp.
Tetraselmis sp.
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Botryococcus braunii
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For this study, we used samples collectedfrom four sites- Nagbacalan (I), Suba (II),Nanguyudan (III) and Sungadan (IV) fromMay to September !!" (Fig# $)# %uring
sampling, rust-colored aggregates of thealgae was fre&uently obser'ed on thelae surface# Microscopic eamination'eri*ed that it was of the oil-rich colonial
green alga, Botryococcus braunii.
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Map of Paoay Lake, Ilocos Norte, Luzon
Is., Philippines, showing the samplingsites 1-4.+nalysis of the collected samples re'ealed that B.braunii colonies were present in all of samplescollected from the four sampling sites during study
time
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Botryococcus braunii is member of the
chlorophyceae (chlorophyta)#
B. braunii is regarded as a potential source of
renewable fuel because of its ability to producelarge amounts of hydrocarbons# %epending onthe strain and growth conditions, up to . ofalgal dry mass can be hydrocarbons#
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/hotosynthetic cultures of B. braunii require01# 0ultures aerated with !#2. 01-enriched
air ha'e a much shorter mass doubling time (3!h) compared with " days for cultures suppliedwith ambient air# 01 enrichment fa'ors theformation of lower botryococcenes (02!402),whereas cultures sparged with ambient air
accumulate higher botryococcenes (0224023)(5olf et al#, $67a)# +pparently, methylationsteps leading from 02! to 02$ and 02 are fasterin 01-enriched cultures than steps leading to
022, 023 and higher homologues# +lthoughautotrophic, B. braunii utilizes exogenous carbonsources for impro'ed growth and hydrocarbonproduction#
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+t present, the production ofphotosynthetic fuel oils is notcompetiti'e with petroleumderi'ed fuels# 1ne reason for this
is a relati'ely slow growth rate ofB. braunii.
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Isochrysis sp#
Isochrysis galbana is a free living marineunicellular phytoagellate of the orderChrysomonadales. i!e other members of thisorder it is rich in polyunsaturated fatty acids 8which are of nutritional 'alue for marine
*sh lar'ae#
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Sparging of cultures with01($.) enrichedair was e'en more
e9ecti'e in increasinggrowth rate and yield, aslong asthe culture medium was
supplemented with ecessFe 2: (Fig# 7), otherwise,Fe 2: star'ationde'eloped rapidly#+pparently ecess 01causesprecipitation of Fe 2: asinsoluble carbonates#+ddition of saturating
concentrations
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;etraselmis sp#
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