Upload
zenaida-ramos
View
224
Download
0
Embed Size (px)
Citation preview
8/8/2019 Micro Pipette Use Age
1/12
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
2/12
Purpose of the Pipettor
Biotechnologists use a
pipettor to transfer
precise amounts of
liquids from one
container to another.
The measu
rements are inmicroliters 1 ml=1000 l.
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
3/12
Care of the Pipettor
Misuse of the pipettor can
result in incorrect
measu
rements affectingyour investigation.
Do not drop the pipettor.
Do not adjust the volume
to less than 2 l.
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
4/12
Parts of the Pipettor
1) yellow pushbutton
2) calibration knob
3) digital volume display
4) barrel
5) disposable pipette tip
6) tip ejector
3
5
6
1
4
6
1
62
3
4
5
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
5/12
Volume AdjustmentStep 1
In the display window are
three vertical numbers.
The top number is tens l(10.0).
The middle number is
ones l (02.0).
The bottom number
tenths l (00.9).
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
6/12
Volume AdjustmentStep 2
Set the pipettor to the
desired volume by turning
the calibration knob until
the volume display readsthe desired numbers.
7.5 l would read from
top to bottom in the
display window:0 - tens
7 - ones
.5 - tenths
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
7/12
Transferring LiquidsStep 1
Adjust the pipettor to the
desired volu
me. Hold the pipettor so the
display is facing the user
and the thumb can easily
depress the yellowpushbutton.
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
8/12
Transferring LiquidsStep 2
Open the box of yellow
pipette tips supplied by the
Office of Biotechnology.
Place the pipette cone (end)
into one of the tips and
press down firmly. NEVER USE THE
PIPETTOR WITHOUT A
TIP!
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
9/12
Transferring LiquidsStep 3
Lift the pipettor and
attached tip away from the
tip box.
Depress the pushbutton to
the first stop before
placing the pipette tip inthe liquid.
Place the tip in the liquid.
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
10/12
Transferring LiquidsStep 4
Slowly release the
pushbutton and draw the
liquid into the tip.
Remove the tip from the
liquid and visually check
for liquid in the tip.
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
11/12
Transferring LiquidsStep 5
Place the tip into the
container which is to
receive the liquid. Slowly depress the
pushbutton as far as it can
go.
With the pushbutton fully
depressed, withdraw the
tip.
Use of the Labnet Micropipettor
8/8/2019 Micro Pipette Use Age
12/12
Transferring LiquidsStep 6
Press down on the tip
ejector button to eject the
tip.
Dispose of the tip in an
appropriate container.
Use of the Labnet Micropipettor