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Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronics Institute of Electronic Appliances and Circuits, University of Rostock, Rostock, Germany. Micro fluidics in PCB technology. University of Rostock PCB-Fluidics. Fluidic PCB-Technology. - PowerPoint PPT Presentation
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Flow injection analysis realized using PCBs S. Gassmann, L. Pagel Faculty of computer since and electronicsInstitute of Electronic Appliances and Circuits, University of Rostock, Rostock, Germany
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Micro fluidics in PCB technology
Electronic area IC's, resistor, capacitor, plug and socket connection, push-button, display, ...
Fluidic area:
pumps, valves, channels, filter, ... cavities, optical sensors, conductivity measurement, …
Electronic area
Fluidic area
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University of RostockPCB-Fluidics
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Fluidic PCB-Technology
normal PCB
production
drilling milling
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Fluidic PCB-Technology
normal PCB
production
etching
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Fluidic PCB-Technology
second PCB stacked
copper lines build channels
gluing
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Fluidic PCB-Technology
tube connection
channel view
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Fluidic PCB-Technology
cross section of channel
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Cross section of channel wall
epoxy
copper (2)
copper (1)channel
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9µC controlled programmable micro pump
Electrical connector- Power 4 –7 V DC- I2C - Bus - programming
500µl/min Flow 150 mBar max Backpressure
over USB-I2C IF fully programmable
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Flow-Injection-Analysis
quantitative measurement of components automated procedure short response times / high sample
throughput usage in quality control / automated
assays developed by J. Růžička and E.H. Hansen
in the year 1975
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Flow-Injection-Analysis
Sample
Reaction coil
Injection valve Detector
Reagent
Pump
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Reaction
Detection of Fe3+
Thiocyanate as reagent forms red colored Iron-Thiocyanate detection by absorption at 480 nm
SCNFe 33
3)(SCNFe
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Why a FIA?
All basic fluidic handling necessary- transportation of fluids- dosing of fluids- mixing- detecting- adoption to detection needed- electronics needed
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FIA in PCB-Technologie Principle
reagentpump
sample pump
reaction coil
injection detector
• 2 separate controllable pumps • no injection valve• optical detection
photometrical 480 nm
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MIXINGMIXINGFlow pattern in 180° curves
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Simulation results of the used reaction channel Simulation results of the used reaction channel
Parameters: 2 pumps with constant flow of 0.2ml/min
Fluidsimulation:FLOWORKS789388 cells
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… and the reality
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Simulation results of the used reaction channel Simulation results of the used reaction channel
parameters:thermopneumatically actuated pumps
with pulsing flow of 0.2ml/min,
789388 cells
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Simulation results of the reaction channelSimulation results of the reaction channel
vector plot of the fluid velocity at the cross section of the channel after the second curve
parameters: thermopneumatically actuated pumps with pulsing flow of 0.2ml/min,
789388 cells
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OPTICAL SENSOROPTICAL SENSOR in FIA
Pho to d io d e
O p tic a l p a th
1m m
Sim ula tio n Flo w 5 m l/m in!
LED
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Optical Cell with air bubble (vector of the force)Optical Cell with air bubble (vector of the force)
Sim ula tio n Flo w 5 m l/m in!
F
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Realization
5 cm
5 cm
• stack of four PCBs • one movable layer of polyimid• electronics on the back of the fourth PCB
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FIA Realization
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FIA in PCB with integrated electronics
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Signals in the photo detector in dependence on the concentration of Fe(III)ions
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PCB FIA facts
Detection Fe3+ Ions0,16 mMol – 10 mMolreproducibility peak height 5%, peak area 10%
Monolithic system consisting fluidics and electronics in one stack of PCBs.
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Thank you for your attention!Thank you for your attention!
