THE 1992 MIAMI BIO/TECHNOLOGY

WINTER SYMPOSIUM

MIAMI SHORT REPORTS VOLUME 2

unsversstAtsbibliothekHANNOVER

TECHNISCHEMFORMATfONSBIBLIOTHEK

Advances in Gene Technology:

Feeding the World in the 21st Century

Proceedings of the 1992 -

Miami Bio/Technology Winter Symposium

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Edited by:

William J.Whelan, Fazal Ahmad, Harvey Bialy, Sandra Black,

Mary Lou King, Mark B.Rabin, Larry P.Solomonson and Indra K.Vasil

NUCLEIC ACIDS

SYMPOSIUM SERIES

No.26

OIRLPRESSat

OXFORD UNIVERSITY PRESS

Oxford New York Tokyo

UB/TIB Hannover

110 534 905

89

CONTENTS

Preface xvii

Acknowledgements xix

PLANT GENE MAPPING AND GENE TRANSFER

MOLECULAR MAPPING OF THE TOMATO GENOME 3

S.D. Tanksley

THE BIOLISTIC PROCESS -ASIMPLE TOOLFORTRANSFORMINGDIVERSE CROP SPECIES 4

J. Sanford

ASSESSMENT OF GENETIC VARIABILITY IN THE OIL PALM

(ELAEIS SVP.) USINGRFLPPROBES 5

S.C. Cheah, L.C.L. Ooi, A.R. Rahimah and M. Maria

COMPARISON OF PERFORMANCE CHARACTERISTICS OF

DIFFERENTBIOLISTIC DEVICES 5

E. Dunder, T. Harris, A. Weissinger, M. Wright, C. Bowman,K. Launis, J. Reed, J. Suttie, S. Jayne, G. Jen and G. Pace

PCR: A USEFULTOOL IN PLANT GENETIC ENGINEERING 6

C. Gutierrez, L. Lopez, P. Molina, A. Lopez, G. de la Riva and

G. Selman-Housein

GENE TRANSFER WITH SUBSEQUENT REMOVAL OF

THE SELECTION GENE FROMTHE HOST GENOME 6

D.W.Ow and E.C.Dale

FOREIGN GENE DELIVERY INTO SWEETPOTATO AND

ITS POSSIBLE FUTURE APPLICATIONS IN AGRICULTURE 7

C.S. Prakash and U. Vardarajan

EXPRESSION OF MULTIPLE GENES INTRODUCED INTO

BLACK MEXICAN SWEET CORN CULTURES BY PARTICLE

BOMBARDMENT 7

D.E. Potts, J.A. Kirihara and KA. Hibberd

MOLECULAR MAPPING OF GENES FOR RESISTANCE TO

BACTERIAL WILT IN TOMATO (L. ESCULENTUM) 8

Z Yu, R.E. Stall and C.E. Vallejos

APPLYING 4 - COLOR FLUORESCENT TECHNOLOGY TO

DNA FINGERPRINT ANALYSIS OFPLANTS GENOMES 8

J.S. Ziegle, S. Koepf and M.N. Kronick

iii

ANIMAL GENE MAPPING AND

GENE TRANSFER

HOMEOBOX GENES IN EVOLUTION AND DEVELOPMENT 11

F.H. Ruddle, M. Murtha, J. Pendleton, S.-W. Lin and J. Leckman

DEVELOPMENT OFASEQUENCE TAGGED SITE BASED BOVINE

GENE MAP 12

A.B. Dietz and J.E. Womack

THE HIGH GROWTH GENE (/ig) IN MICE IS LOCATED IN

CHROMOSOME 10 LINKED TO Igf-1 12

J.F. Medrano, D. Pomp, B.A. Taylor and E. Bradford

CHEMILUMINESCENT DETECTION OFSINGLE COPY GENE BY

A 2-STEP HYBRIDIZATION METHOD 13

Q. Nguyen, F. Witney and A. Tumolo

EXPRESSION OF TWO MINI-GENE VERSIONS OF THEHUMAN PRO alpha 1 (I) GENE IN TRANSGENIC MICE:

EFFECT OF DELETING PUTATIVE REGULATORY

SEQUENCES IN THE FIRSTINTRON 13

B.P. Sokolov, P.K. Mays, J.S. Khillan and D.J. Prockop

THE EFFECT OF THE PARTIAL DELETION OFTHE Y

CHROMOSOME IN MICE ON GENOMIC IMPRINTING IN

THE PROGENY 14

J. Styrna

ADDITION OF 43 INFORMATIVE COMPARATIVEANCHOR LOCITO THE BOVINE SYNTENY MAP 14

J.E. Womack, A.B. Dietz, D.S. Gallagher, L. Li, N. Zhang,H.L. Neibergs, Y.D. Moll and A.M. Ryan

MOLECULAR BIOLOGY OF PLANT

DEVELOPMENT

MOLECULAR SWITCHES FOR GENE EXPRESSIONDURING PLANT DEVELOPMENT 17

N.-H. Chua, Philip Gilmartin, Kazuyuki Hiratsuka and

Xiao-DongWu

IDENTIFICATION OF GENES SPECIFICALLY EXPRESSEDIN DEVELOPING ARABIDOPSIS EMBRYOS BY TAGGING

WITH AT-DNA CONTAININGAPROMOTERLESS GUS A

REPORTER GENE 18

M. Devic, K. Lindsey, M. Delseny and P. Gallois

iv

MEMBERS OF THE CAB MULTIGENE FAMILY SHOWDIFFERENTIAL EXPRESSION DURINGMATURATIONIN EASTERN LARCH 18K.W. Hutchison, P.B. Singer and M.S. Greenwood

AUTOMATED NUCLEIC ACID PURIFICATION OFPLANTTISSUE 19S.M. Koepf and C. Lamb

ISOLATION OF PURINE BIOSYNTHETIC GENES OFARABIDOPSIS THALIANA BY COMPLEMENTATIONOFESCHERICHIA COLIAUXOTROPHS 19

J.F. Senecoff and R.B. Meagher

DEVELOPMENTALLY REGULATED SEED SPECIFICGENES IN PEANUT 20O.G. Paik-Ro, R.L. Smith and D.A. Knauft

INVERTASE ACTIVITY IN DEVELOPING PEANUT SEEDS 20

Y.-C. Chen, R.L. Smith and D.A. Knauft

THE 65KD AUXIN BINDINGPROTEIN IS ADNABINDINGPROTEIN 21M-J. Wu, C.F. Horton and J.A. Knopp

IN SITUTRANSIENTEXPRESSION OF A GUS-GENE INPLANT CELLS BY APARTICLE GUN DRIVEN BYCOMPRESSED NITROGEN GAS 21

H. Kakuta, T. Seki, M. Matui, T. Anai, K. Hasegawa and J. Mizutani

MOLECULAR BIOLOGY OF ANIMAL

DEVELOPMENT

CREATION OF MICE WITHTARGETED DISRUPTIONS INPROTO-ONCOGENES AND HOMEOBOX GENES 25M.R. Capecchi

BOUNDARIES OF HOMEOTIC GENE EXPRESSION IN

THE DROSOPHILA EMBRYO 26

M. Bienz and J. Miiller

HIGH AFFINITY DNA-PROTEININTERACTIONS OF THECELLULAR ETSl PROTEIN: THE DETERMINATION OFTHE ETSl BINDING MOTIF BY A GENERAL METHOD 27

R.J. Fisher, G. Mavrothalassitis, A. Kondoh and T.S. Papas

HMG-CoA REDUCTASE AND FARNESYL PYROPHOSPHATE

SYNTHETASE GENE EXPRESSION IN DEVELOPING RAT BRAIN 27G.C. Ness

v

TRANSCRIPTIONAL REGULATION AND SPECIFIC DNA

BINDING OF THE DEVELOPMENTALLYREGULATED

ets PROTEIN 28

A. Seth, R. Ascione, D. Thompson, A. Panayiotakis, H. Li,D. Watson and T.S. Papas

INSULIN AFFECTS DEVELOPMENTAL EXPRESSION OF

GLUTAMIC ACID DECARBOXYLASE IN CULTURED

EMBRYONIC NEURONS 28

B.H. Shah and R.E. Hausman

IDENTIFICATION OF POSSIBLE LINEAGE-SPECIFIC

TRANSCRIPTIONALACTIVATORS OF THE CAENORHABDITISELEGANS ges-1 GENE 29

V.L. Stroeher and J.D. McGhee

GENDER DETERMINATION BY PCR 29

A. Vader, I. Bakke, P.J. Palsboll and M.R. El-Gewely

DETECTION OF ERYTHROID PROGENITORS IN HUMAN

PERIPHERAL BLOOD BY CYTOKINE RECEPTORTRANSCRIPT PHENOTYPING 30

D.M. Williams, F. -S. Xu, G.P. Rodgers, A.N. Schechter, C.T. Noguchiand W.D. Hankins

CELL CULTURE AND GENETIC

MANIPULATION OF PLANTS

CELL CULTURE AND GENETIC MANIPULATION OF CEREALS 33

I.K. Vasil and V. Vasil

TISSUE CULTURE OF ABRUS PRECATORIUS AND IN VITRO

ABRUS LECTIN PRODUCTION: A NEW REPORT 34

P.S. Bhattacharya, T.K. Maiti and B.C. Bhattacharya

REGENERATION OF MUCUNA PRURIENS 34S. Chattopadhyay, S. Chatterjee and S.K. Datta

TRANSFER OF A HERBICIDE RESISTANT GENE IN

SUGARCANE VIA MICROPROJECTILE BOMBARDMENTOF CELL SUSPENSION CULTURES AND ELECTROPORATIONOFPROTOLASTS 35M.K.U. Chowdhury and I.K. Vasil

PLANTLETS REGENERATION FROM LEAF CALLUS OF

CURLY - BIRCH 35

O. Ditmar

PLANT REGENERATION AND TRANSFORMATION IN

SOME GRAIN LEGUMES 36S. Eapen, L. George, F. Kohler, C. Golz and 0. Schieder

VI

STUDY ON POSSIBLE FUNCTION OF NP24 INPLANTSALINITY TOLERANCE 36

D. Fu gui, W. Hong xin and H. Zhi ang

MORPHOGENESIS IN SOME FAST GROWING TREES 37

M.I. Hoque, A. Ara and R.H. Sarker

SALT STRESS IN CALLUS CULTURES OF RICE (ORYZA SATIVA L) 37

P.B. Kavi Kishor and G.M. Reddy

SOME ASPECTS OF PLANTDIFFERENTIATION IN CALLUSCULTURES OF RICE 38

P.B. Kavi Kishor and G.M. Reddy

THE CHLOROPHENOL RED ASSAY - APH INDICATOR TEST

TO IDENTIFY MAIZE TRANSFORMANTS CONTAINING THEBAR GENE 38

C. Kramer, R.D. Shillito and J.J. DiMalo

REGENERATION OF PETAL CALLUS IN PUNICA GRANATUM 39

D.M. Mahishi, P.R. Sudha and C.S. Prakash

SOMATIC EMBRYOGENESISAND PLANT REGENERATIONIN COTYLEDON CULTURES OF BITTER GOURD

(MOMORDICA CHARANTIA L.) 39

B. Mallaiah and J. Nizam

PARAMETERS AFFECTING TRANSFORMATION EFFICIENCYOF LEAF EXPLANTS IN PEANUT (ARACHIS HYPOGAEA L)BY AGROBACTERIUS TUMEFACIENS 40

E. Mansur, C. Lacorte, D.E. Oliveira, B. Timmerman, V.G. de Freitas,M. Louzada and A.R. Cordeiro

DIRECT SOMATIC EMBRYOGENESIS FROM AXES OF

MATURE PEANUTEMBRYOS 40

A.H. McKently

REGENERATION OFPEANUT AND PERENNIAL PEANUT FROM

CULTURED LEAF TISSUE 41

A.H. McKently, G.A. Moore and F.P. Gardner

PRODUCTION OF HIGH YIELDING LINES THROUGH TISSUE

CULTURE IN HELIANTHUS ANNUUS. L. (SUNFLOWER) 41

P.K. Ram

ISOLATION OF HIGH YIELDING LINE THROUGH TISSUE

CULTURE IN CARTHAMUS TICTORIOUS L. (SAFFLOWER) 42

M.M. Reddy

NaCl-TOLERANT RICE PLANTS FROM NaCl-TOLERANT RICE

CELL LINES OBTAINED THROUGH ANTHER CULTURE 42

P. Sathish andMW. Nabors

VII

GENETIC HYDBRIDIZATIONAND MANIPULATION IN THE

TROPICAL GRASS GENUSPENNISETUM (HYBRIDS BETWEEN

PENNISETUM GLAUCUMAND P. PURPUREUM) 43

S.C. Schank, D. Diz and R.L. Smith

THE EFFECTS OF MICROPROJECTILE BOMBARDMENT

ON IMMATURE EMBRYOS OF PEARL MILLET (PENNISETUMGLAUCUML.R.BR.) 43

M.G. Taylor and I.K. Vasil

BIOTECHNOLOGY AND LIVESTOCK

IMPROVEMENT

GENE DELIVERY IN WHOLE ANIMALS 47

T.E. Wagner

TARGETING THE EXPRESSION OF FOREIGN PROTEINS TO

THE MAMMARY GLAND 48

A.J. Clark, A.L. Archibald, M. McGenaghan, J.P. Simons,C.B.A. Whitelaw and J. Wilmut

PORCINE SOMATOTROPIN (pST) AND MORE EFFICIENT AND

LEANERPORK PRODUCTION 49

C.A. Baile, F.C. Buonomo, J. Klindt and C.L. McLaughlin

CLONING AND EXPRESSION OFTHE ALEUTIAN DISEASEVIRUS 85 KILODALTON (VP-1) STRUCTURAL PROTEIN 49

R. Black, R. Robison, D. Robertson, F.B. Johnson and R.W. Leavitt

INTEGRATION AND GERMLINE TRANSMISSION OFAHYBRID: BOVINE ALPHA Si CASEIN PROMOTER/hTPAcDNA IN TRANSGENIC RABBITS 50

F.O. Castro, J. Limonta, R. De Armas, R. Solano, A. Aguilar,P. Puentes, R. Lleonart, J. De la Fuente and A. Perez

PRODUCTION OF CFTRIN THE MILK OF TRANSGENIC MICE 50

P. DiTullio, S. Cheng, J. Marshall, R. Gregory, K. Ebert,H. Meade and A. Smith

IMPROVED EFFICIENCY OF INCORPORATION OF DONORBLASTODERMAL CELLS IN CHIMERIC CHICKENS ANDTHEIR DETECTION USING A FEMALE-SPECIFIC DNA PROBE 51

R.J. Etches, R.S. Carsience, D.L. Shaw and S.M. Verrinder Gibbins

GENE ACTIVATION ASSOCIATED WITH FEEDINGIN

NEWLY EMERGED HORN FLIES, HAEMATOBIAIRRITANS 51

F.D. Guerrero

viii

SECRETION OFPROKARYOTIC ENZYMES INTO THEGASTROINTESTINAL TRACT OF TRANSGENIC ANIMALS 52J. Hall, MA. Surani, G.P. Hazlewood, B.H. Hirst and H.J. Gilbert

CLONINGAND EXPRESSION OF BOVINE GROWTHHORMONE cDNA 52A. Martinez-Torres, L.E. Alvidrez-Quihui, V. Ramirez-Anguloand V. Barrera-Saldana

EXOGENOUS CLONED DNA CANPENETRATE LPTINGSPERM CELLS 53A. Rodriguez, F.O. Castro, I. Guillen, A. Perez, O. Hernandez,M. Duenas, R. Solano, N. Baranosky, V. Falcon, R. Martinez,A. Aguilar, R. Lleonart and J. de la Fuente

EXPRESSION AND SECRETION OF A BACTERIALENDOGLUCANASE IN POLARIZED INTESTINAL EPITHELIA 53K.L. Soole, J. Hall, G.P. Hazlewood, H.J. Gilbert and B.H. Hirst

MOLECULAR BIOLOGYAND CROP

IMPROVEMENT

STATE OF THE ART OF GENE TRANSFER TO PLANTS 57I. Potrykus

CHARACTERIZATION OF cDNA CLONES OF DROUGHT-INDUCED

GENES IN ATRIPLEX CANESCENS (SALTBUSH) 58

J. Chen, J. Cairney, R.J. Newton andE.A. Funkhouser

PRODUCTION OF TRANSGENIC POTATO PLANTSENGINEERED FORRESISTANCE TO PVX BY EXPRESSIONOF THE COATPROTEIN GENE: THE EGYPTIAN EXPERIENCE 58

M. Eweida, T. Nasr El-Din and M.A. Madkour

OCCURRENCE OF PLANT CELLREGULATING METABOLITESPRODUCED BY STRAINS OFRHODOCOCCUS 59

J.L. Ibave, A.C. Gonzalez, E. Portillo, J.J. Molina and J.C. Lopez

EXPRESSION OF ATYPE HI FISH ANTIFREEZE PROTEIN GENE

IN TRANSGENIC TOBACCO IMPROVES PLANT FREEZE

TOLEREANCE 59

K.D. Kenward, P.L. Davies, W. Downing, M. MacHutchonand J.C. McPherson

THE USE OF INTACT CELL ELECTROPORATION FOR THE

TRANSFORMATIONAND REGENERATION OF SUGAR CANE 60

P. Molina, A. Arencibia, C. Gutierrez, A. Fuentes, E. Menendez,V. Grenidge, G. de la Riva and G. Selman-Houssein

SEX DETERMINATION AND NUTRITIONAL INFLUENCE ON SEX

EXPRESSION INJOJOBA (SIMONDSIA CHINENSIS L.) 60

P.K. Ram

IX

THE MOLECULAR GENETIC, BIOCHEMICAL, AND PHYSICALANALYSIS OF STARCH SYNTHESIS IN BARLEY: A NORDIC

COLLABORATION 61

A.H. Schulman, M. Bojko, A. Deiber-Haag, C. Jansson, LA. Kleczkowski,J. Marcussen, M.S. Motawia, B.L. Moller, P. Olesen, C.E. Olsen,O.-A. Olsen, E. Pessa, K. Poutanan, C. Sun, T. Suortti, J. TyynelaandP.Villand

CLONING AND SEQUENCING OF cDNA CLONES ENCODING

GAMMA-KAFIRIN PROTEIN FROM SORGHUM BICOLOR 61

K. Takasaki, E. de Barros, S. Kirleis and B. Larkins

LIPID METABOLISM IN GERMINATING BARLEY 62

B.E. Valk, R. Bakhuizen, A. Doderer, J.C. Heistek, W.L. Holtman,I. Kokkelink, J.R.V. Mechelen, M. Smits and A.C. Douma

ISOLATION AND CLONING OF THE ANDEAN POTATO

MOTTLE VIRUS COAT PROTEIN GENES 62

A.C.P. Vicente, N.Shindo, M. Weyne, P.S.T. Brioso, R. Krengiel,D.E. Oliveira and B. Timmerman

MICROBIAL AND MARINE BIOTECHNOLOGY

TRANSGENIC FISH AND AQUACULTURE 65

T.T. Chen, R.A. Dunham and D.A. Powers

MICROBIAL TECHNOLOGIES IN THE SERVICE OF

INTERNATIONAL CO-OPERATION 66

E.J. DaSilva

FACTORS INVOLVED IN STRUCTURE-FUNCTIONRELATIONSHIPS OF THE HEME DOMAIN OF NITRATE

REDUCTASE 67

A.C. Cannons, M.J. Barber and L.P. Solomonson

ELECTROCHEMICAL ANALYSIS OF ASSIMILATORY NITRATEREDUCTASE 67

M.J. Barber, C.J. Kay, B.A. Notton and L.P. Solomonson

TRANSGENIC SALMONWITH ENHANCED GROWTH ANDFREEZE RESISTANCE 68C. Hew, S.J. Du, Z. Gong, P. Davies, M. Shears and G. Fletcher

GENERATION OF TRANSGENIC TILAPIA WITH A CHIMERICGENE THAT DIRECTS THE SYNTHESIS OF tiGH mRNA

IN CHO CELLS 68R. Martinez, D. Garcia del Barco, 0. Hernandez, R. Lleonart,F. Herrera, E. Cabrera and J. de la Fuente

x

COMPARATIVE INVESTIGATIONS OF THE CELL WALLS OFTHE

DISSOCIATIVE FORMS / M,R,S / OF BACILLUS SUBTILIS 76,PRODUCER OF PROTEOLYTIC ENZYME COMPLEX WITH

MILK - CLOTTING ACTION 69

K. Petrova, D. Spassova, T. Antonova and T. Nikoevska

ENGINEERED CROPS

COMMERCIAL PRODUCTION OFTRANSGENIC SOYBEANAND RICE PLANTS FROM ELITE CULTTVARS USING

ELECTRIC DISCHARGE PARTICLE ACCELERATION 73

P. Christou

TRANSGENIC RICE PLANTS FOR STUDIES IN GENE

REGULATION AND CROP IMPROVEMENT 74

K. Shimamoto

TRANSGENIC CUCUMBER PLANTS THAT EXPRESS THE

COAT PROTEIN GENE OF CUCUMBER MOSAIC VIRUS:

FIELD TESTING PROTECTION 75

P.C. Chee, J.L. Slightom and D. Gonsalves

CUSTOMPOLYMERASE CHAINREACTION ENGINEERING

SEED SPECIFIC PLANT EXPRESSION CASSETTES USING

CLONED AND GENOMIC DNAS 75

R.F. Drong and J.L. Slightom

THREE-DIMENSIONAL STRUCTURES OF TWO SEED STORAGE

PROTEINS FROMTHE JACK BEAN 76

T.-P. Ko, J.D. Ng, M.S. Greene and A. McPherson

THE CLONING AND EXPRESSION OF CANAVALIN FROM

THE JACK BEAN PLANTIN ESCHERICHIA COLI 76

J.D. Ng, T.-P. Ko and A. McPherson

CHARACTERIZATION OF EPSPS ENZYMES AND THEIR USE

IN THE PRODUCTION OF ROUNDUP® TOLERANT SOYBEAN 77

D. Re, S. Padgette, X. Delannay, B. LaVallee, D. Eichholtz, G. Barry,M. Weldon and G. Kishore

VETERINARY VACCINES

TRANSFER OFTECHNOLOGIES IN MOLECULAR BIOLOGY

TO DEVELOPING COUNTRIES: RECOMBINANTVACCINES

FOR RINDERPEST 81

T. Yilma

POXVIRUS VECTORS USEFUL IN VETERINARY MEDICINE 82

E.Paoletti

xi

N.M.R. AND CD STUDIES OF AN IMMUNOGENIC PEPTIDEFROM A WHEATPROTEIN 83

M.J.C. Alcocer, E.N.C. Mills, B.J. Goodfellow, I.J.C. Colquhounand M.R.A. Morgan

DEVELOPMENT OF A RECOMBINANT VACCINE FORPASTEURELLAHAEMOLYTICA 83

A. Potter and R. Harland

IMMUNOPROTECTION IN MICE AGAINST HEMOFLAGELLATEPARASITIC PROTOZOA (TRYPANOSOMA BRUCEIBRUCEI,TRYPANOSOMA CRUZI, AND LEISHMANIA MEXICANA) BYCLONED SEQUENCES OF TRYPANOSOMA BRUCEIRHODESIENSEEXPRESSED IN E. COLI 84

C. Powell

ANTIBODTES AGAINST INSECT VECTORS MODULATE

DISEASE TRANSMISSION 84

R. Ramasamy, K. Srikrishnaraj, B. Jesudasan, C. Ranasingheand M.S. Ramasamy

FOOD INITIATIVES FOR THEDEVELOPING WORLD

FUTURE INITIATIVES IN BIOTECHNOLOGY RESEARCHFOR TROPICAL AGRICULTURE: THE CASE OF CASSAVA 87W.M. Roca, F. Angel, R. Sarria, J. Mayer, J. Tohme, A. Mejiaand G. Mafia

THE MANZANAR, MULLET, MILKFISH AND MOSQUITEPROJECT IN ERITREA 88G.H. Sato

MOSQUITO BIOCONTROL BY THE 8-ENDOTOXIN GENESOF BACILLUS THURINGIENSIS VAR. ISRAELENSIS CLONEDIN AN AMMONIUM-NITROGEN-FIXINGCYANOBACTERIUMANABAENA SIAMENSIS 89S. Boussiba and A. Zaritsky

MAPPINGAGRONOMICTRAITSIN GRAIN LEGUMES WITH RFLPS 89C.A. Fatokun and N.D. Young

LOCATING GENETEIC FACTORS CONTROLLING RHIZOBIUMNODULATION INTENSITY ON THE RFLP MAP OF COMMON BEAN(PHASEOLUS VULGARIS) 90P. Gepts, R.O. Nodari and S.M. Tsai

ISOLATION OF A SCYTALIDIUM ACIDOPHILUM STRAIN WITHENHANCED ADAPTABILITY TO HUMIC ACID CONCENTRATION:EFFECTS ON THE FUNGAL GROWTH 90A.M. Martin

XII

ANIMAL AND FOOD DIAGNOSTICS

DNA PROBES FOR ANIMAL HEALTH APPLICATIONS 93E.F. Workman, P. Andersen, D. Ludtke, P. Tyrrell, A. Siefring and C. Vary

DNA PROBE ASSAYS FOR FOOD PATHOGENS 94G.H. Parsons

EARLY DIAGNOSIS OF BOVINE LEUKAEMIAVIRUS (BLV)INFECTION BY PCR 95A. Agresti, R. Meneveri, A. Marozzi, G. Poli and E. Ginelli

NORMAL cDNA SEQUENCE FOR BOVINE CD18 AND

IDENTIFICATION OF A POINT MUTATION THATCAUSES BOVINE LEUKOCYTE ADHESION DEFICIENCY 95D.E. Shuster and M.E. Kehrli, Jr.

NOVEL PROCEDURE FORTHE QUANTITATIVE ANALYSISOF DNA STRAND BREAKS INDUCED IN VIVO BY OXIDATIVEAGENTS 96A. Tremblay and M.-E. Mirault

PLANTS AS BIOREACTORS

EVALUATION OF IMMUNOGLOBULIN PROCESSINGINPLANT CELLS 99

A. Hiatt

PLANT MOLECULAR FARMING AND ITS APPLICATIONS 100J. Pen, A.J.J, van Ooyen, P.C. Sijmons, P.J.M. van den Elzenand A. Hoekema

SYNTHESIS OF CERTAIN OILATILESINCALLUS CELLS OF

CLOVE (EUGENIA CAR YOPHYLLA L.) 101

V.C. Dwadash-Shreni

AROMATASE INHIBITORS IN EDIBLE TROPICAL PLANTS 101

M.T. Kowalska, Y. Itzhak, D. Cristante and D. Puett

ENGINEERING STEARATE LEVELS IN CANOLA OIL THROUGHTHE USE OF ANTISENSE STEAROYL-ACP DESATURASE GENE

CONSTRUCTS 102

D.S. Knutzon, G.A. Thompson, S.E. Radke, V.C. Knauf, E.R. SveeandJ.C.Kridl

ENGINEERING LAURATE PRODUCTION IN OILSEEDS 102

T.A. Voelker, A.C. Worrell, L. Anderson, J. Bleibaum, C. Fan,D.J. Hawkins andH.M. Davies

XIII

PLANT PATHOGENESIS AND HOST DEFENSE

MECHANISMS

MECHANISMS FORPLANT RESISTANCE TO MICROBIALATTACK: ELUCIDATION AND MANIPULATION 105

C.J. Lamb

TOMATO GENES FOR RESISTANCE TO CLADOSPORIUMFULVUM: A USEFUL MODEL FOR UNDERSTANDING RACE

SPECIFIC PLANT PATHOGEN INTERACTIONS 106

J.D.G. Jones, D. Jones, G. Bishop, C. Thomas, M. Dickinson, K. Harrison,P. Balint-Kurti, K. Hammond-Kosack and T. Ashfield

COMPARISON OF DEFENSE RESPONSES BETWEEN

RESISTANT (BINTJE) AND SUSCEPTIBLE (DELTA)POTATO CULTIVARS (CVS.) TO ANDEAN POTATO

MOTTLE VIRUS (APMV) 107

T. Jacinto, P. Brioso, 0. Machado and B. Timmerman

STRUCTURE OF A MOUSE GENE ENCODING A MALE

GERM CELL-SPECIFIC PROTEIN WITH SEQUENCESIMILARITY TO PLANT "PATHOGENESIS-RELATED"PROTEINS 1 107

M. Kasahara and N. Mizuki

ALTERNATE INTLATION SITES IN A TOMATO PAL GENE

RESPONDING TO DIFFERENT ENVIRONMENTAL STIMULI 108

S.-W. Lee, E.J. Robb and R.N. Nazar

MOLECULAR STUDIES OF THE HOST/PATHOGEN INTERACTIONS

BETWEEN BARLEY AND BARLEY POWDERY MILDEW (ERYSIPHEGRAMINIS) 108

H. Thordal-Christensen, J. Brandt, M. Naesby and D.B. Collinge

STUDY ON PARTIAL GENOMIC STRUCTURE OF ARROWHEAD

PROTEINASE INHIBITOR A AND B BY THE POLYMERASE

CHAIN REACTION (PCR) METHOD 109

X. Wen-feng, G. Zhen-zhen, Y. Wan-xia and Q. Zheng-wu

XIV

FEEDING THE WORLD IN THE 21st CENTURY:THE ROLE OF BIOTECHNOLOGY

PROGRESS TOWARDSTHE COMMERCIALIZATION OFGENETICALLY ENGINEERED CROPS 113R.T. Fraley

NEW TECHNOLOGIES FORPROPAGATION AND GENETICIMPROVEMENT OF LIVESTOCK 114

N. First

MOLECULAR GENETICS FOR IMPROVED PRODUCTIVITYAFORWARD LOOK 116R.B. Flavell

DEVELOPMENTAND IMPACT OF TRANSGENIC ANIMAL

TECHNOLOGY 117J.J. MacQuitty

Author Index 119

Subject Index 123

xv