mailto:rdf@ebi.ac.uk

Metagenomics: applied to a broad range of environments

DNA

extraction

Sequencing

mRNA

extraction

PCR

AmpliconMetatranscriptome Metagenome

A free to use resource for the archiving,

assembly, analysis, & browsing of

microbiome data

AnalysisData archiving Assembly

Assembly

Binning

Reference based and de novo

Different way to analyse shotgun metagenomics

Amplicon

& raw read analysis

• Recent advances

• Increased sequencing depth

• Reduced chimeric sequences

• Reduced computational cost

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7

$ mg-toolkit sequence_search -s my.fasta

Nature 2018 Jun;558(7711):595-599