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SDMS DocID 000234829

Benthic Invertebrate Evaluation For the Assabet River

WR Grace Site Action Massachusetts

Prepared For

GeoTrans Inc 6 Lancaster County Road

Harvard MA 01451

Prepared By

tAJ

Katherine A Fogarty Project Manager

Menzie-Cura amp Associates Inc 8 Winchester Place Suite 202

Winchester MA 01890

-Susan Kane Driscoll PhD Project Reviewer

T

1tsJ tt

October 27 2003

MCA-668

MENZIE bull CURA amp ASSOCIATES INC Environmental Consultants

8 WINCHESTER PLACE SUITE 202 bull WINCHESTER MASSACHUSETTS 01890 bull (781) 756-1600 bull FAX (781) 756-1610 bull wwwMENaECURAcoM

MENZIE bull CURA amp ASSOCIATES INC Environmental Consultants

8 WINCHESTER PLACE SUITE 202 bull WINCHESTER MASSACHUSETTS 01890 bull (781) 756-1600 bull FAX (781) 756-1610 bull WWWMENZIECUHACOM

Job No 668V October 27 2003

Mr Derrick Golden Mr Daniel Keefe Waste Management Division Bureau of Waste Site Cleanup US Environmental Protection Agency MA Department of Environmental Region I Protection 1 Congress Street Suite 1100 One Winter Street Boston MA 02114-2023 Boston MA 02108

Subject Benthic Invertebrate Evaluation for the Assabet River W R Grace Site Acton Massachusetts

Gentlemen

The enclosed report presents an evaluation of the benthic invertebrate data collected from the Assabet River in August 2003 The sampling was performed at the request of the Government Parties according to the approved workplan In an e-mail of March 24 2003 Derrick Golden of USEPA Region 1 expressed to Maryellen Johns of WR Grace the Government Parties concern that the benthic invertebrates in the pore water of sediments need to (be) further evaluated to determine if there are any unacceptable risks(s) to the benthic invertebrate community The enclosed report addresses this concern

If you have any questions or comments regarding this report please call me at 781-782-6141

Sincerely

Katherine A Fogarty PET Project Manager

Enclosures

cc Stephen Anderson Anderson amp Kreiger

October 27 2003 Anne Sheehan GeoTrans Jim Deming Acton Water District Michael LeBlanc MADEP Bob Eisengrein ACES Doug Halley Town of Acton Maryellen Johns Remedium Group Mary Michelman ACES Michael Moore Town of Concord Board of Health Chuck Myette Brown amp Caldwell Mitch Obradovic Remedium Group Jim Okun OTampO Barbara Weir Metcalf amp Eddy Acton Public Library

I1 bullraquo S2 laquolaquoX

Memorandum

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION I ONE CONGRESS STREET SUITE 1100

BOSTON MASSACHUSETTS 02114-2023

Date November 13 2003

bullSubj Benthic Invertebrate Evaluation for the Assabet River for the WR Grace Superfund Site

located in Acton Massachusetts

From Derrick S Golden - Remedial Project Manac

I To James Coles shy Ecological Risk Assessor

I cc Bart Hoskins shy Ecological Risk Assessor

Ii

I

Please find attached your copy of the Benthic Invertebrate Study that was prepared by Menzie-Cura amp Associates Inc

This study was performed on behalf of WR Grace as part of the Remedial InvestigationFeasability Study (RIFS) for groundwater at the WR Grace Site in Acton Massachusetts

1

I would appreciate your review thoughts and or comments on this Evaluation

If you have any questions or concerns I may be reached at 617-918-1448

Thank you

1

2

3 4

5

6

TABLE OF CONTENTS

10 INTRODUCTION 1

20 FIELD SAMPLING

30 DATA EVALUATION

31 RESULTS 32 STATISTICAL EVALUATION

40 CONCLUSIONS

50 REFERENCES

10 INTRODUCTION

Menzie-Cura amp Associates Inc sampled benthic invertebrates in the Assabet River on August 4 2003 according to our letter of June 23 2003 that was approved by the Government Parties in their letter of July 31 2003 The purpose of this sampling was to evaluate whether Volatile Organic Compounds (VOCs) in groundwater discharging to the Assabet River at Transect 6 are having an adverse affect on the abundance and diversity of the population of benthic invertebrates in the river In an e-mail of March 24 2003 Derrick Golden of USEPA Region 1 expressed to Maryellen Johns of WR Grace the Government Parties concern that the benthic invertebrates in the pore water of sediments need to (be) further evaluated to determine if there are any unacceptable risks(s) to the benthic invertebrate community

Transect 6 is the location where the highest concentrations of VOCs have been detected in groundwater located approximately six inches below the sediment surface water interface VOCs detected in groundwater at Transect 6 (ASBRV-T6) include 11shydichlorothene or vinylidene chloride (VDC) at concentrations up to 420 ugL and vinyl chloride at concentrations up to 100 ugL VOCs detected in groundwater samples collected beneath the Assabet River at Transects 1 through 6 (ASBRV-T1 through ASBRV-T6) are included in Table 1 and their locations are shown on Figure 1 VOCs have not been detected in surface water samples collected in the Assabet River as part of the Remedial Investigation

20 FIELD SAMPLING

Benthic invertebrate samples were collected at the following stations in the Assabet River

bull ASBRV-T3 Transect 3 a location along the WR Grace site where VDC was not detected in groundwater beneath the river

bull ASBRV-T6 Transect 6 where the highest concentration of VDC was detected in groundwater beneath the river and

bull ASBRV-UP7 a location upstream of the WR Grace site and Route 62

Figure 1 shows these locations in relationship to the Grace property

Five replicate benthic invertebrate samples per station were collected with a Surber sampler Water quality parameters of pH dissolved oxygen specific conductance and temperature (Table 2) were also measured at each station The five replicate sampling locations were selected to have as similar a benthic substrate as possible The sediment type at each location consisted of gravel and small cobbles Although there was aquatic vegetation near many of the sampling locations we tried not to sample directly in a stand

of vegetation Most of the submerged vegetation we observed was awlleaf arrowhead (Sagittaria subulata) The benthic invertebrate samples were sent to Aquatec Biological Services of Williston Vermont for identification to the lowest taxonomic level practicable (usually species or genus) and enumeration Their report is attached as Appendix A

30 DATA EVALUATION

The following metrics were calculated for the data total abundance total number of taxa dominant species fraction Shannon-Weiner Index Evenness and Ephemerotpera Plecotera Tricoptera Index The results are in Table 3

bull Abundance is a direct count of the number of individual organisms The number of individuals is a measure of the standing stock of the benthic community and provides an indication of the ability of the benthic community to serve as a prey base for higher trophic levels

bull Taxa richness is a count of the number of different taxa (in most cases taxa are counted at the level of the species) The assumption is that high diversity is indicative of a benthic community with a greater diversity of microhabitats and a broader range of species with varying tolerances to physical biological and chemical interactions

bull Dominant species fraction is the ratio of the total number of individuals from the most common species divided by the total abundance for each station As with taxa richness the assumption is that a benthic community that is not diverse and is dominated by one or more species reflects a community with a more narrow range of adaptations and tolerances to physical biological and chemical interactions Smaller values of this metric represent a more diverse benthic community

bull The Shannon-Weiner Diversity Index (H) provides another useful comparative measure of benthic community structure (Begon et al 1990) The index is species-based and assesses diversity using both the number of species (species richness) and the number of individuals (abundance) per species Although more complex statistical testing can be employed to compare H statistics generally the greater the Shannon-Weiner Diversity Index the greater the diversity of benthic species at a station

The Shannon-Weiner Index is calculated as follows

=-gt log p Where

p = frequency of species i in the sample - N N~

N = number of individuals of species i

N = total individuals in sample S = number of species

bull Evenness Index (Pielous J) provides another useful comparative measure of benthic community health as some research has indicated that this index may be the aspect of community structure most strongly affected by response to chemical contamination (Dr E Gallagher personal communication) Similar to the Shannon-Weiner Index the Evenness Index is species-based and assesses the relative abundance of individuals among the species Generally the greater the Evenness Index the more equitable the distribution of individuals among the species

The Evenness Index is calculated as follows

max

Where

H = Shannon-Weiner Diversity Index H max = log of k (number of taxonomic groups)

bull EPT INDEX

An EPT Index was calculated as the number of taxa within the orders Ephemeroptera Plecoptera and Tricoptera at each station in comparison to the upstream reference This index summarizes taxa richness within the insect orders that are considered sensitive to pollution (Plafkin et al 1989)

31 Results

Figures 2 through 6 are box and whisker plots of the metrics abundance number of taxa Shannon-Weiner Index dominant species fraction or evenness per sampling station

Abundance

As shown in Figure 2 total abundance is highest in the upstream sample while abundance at ASBRV-T3 and ASBRV-T6 are within the same range as each other

Number of Taxa

Figure 3 shows a large range in the number of taxa at ASBRV-T3 Number of taxa at ASBRV-T6 and the upstream sample fall within this range

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

I1 bullraquo S2 laquolaquoX

Memorandum

UNITED STATES ENVIRONMENTAL PROTECTION AGENCY REGION I ONE CONGRESS STREET SUITE 1100

BOSTON MASSACHUSETTS 02114-2023

Date November 13 2003

bullSubj Benthic Invertebrate Evaluation for the Assabet River for the WR Grace Superfund Site

located in Acton Massachusetts

From Derrick S Golden - Remedial Project Manac

I To James Coles shy Ecological Risk Assessor

I cc Bart Hoskins shy Ecological Risk Assessor

Ii

I

Please find attached your copy of the Benthic Invertebrate Study that was prepared by Menzie-Cura amp Associates Inc

This study was performed on behalf of WR Grace as part of the Remedial InvestigationFeasability Study (RIFS) for groundwater at the WR Grace Site in Acton Massachusetts

1

I would appreciate your review thoughts and or comments on this Evaluation

If you have any questions or concerns I may be reached at 617-918-1448

Thank you

1

2

3 4

5

6

TABLE OF CONTENTS

10 INTRODUCTION 1

20 FIELD SAMPLING

30 DATA EVALUATION

31 RESULTS 32 STATISTICAL EVALUATION

40 CONCLUSIONS

50 REFERENCES

10 INTRODUCTION

Menzie-Cura amp Associates Inc sampled benthic invertebrates in the Assabet River on August 4 2003 according to our letter of June 23 2003 that was approved by the Government Parties in their letter of July 31 2003 The purpose of this sampling was to evaluate whether Volatile Organic Compounds (VOCs) in groundwater discharging to the Assabet River at Transect 6 are having an adverse affect on the abundance and diversity of the population of benthic invertebrates in the river In an e-mail of March 24 2003 Derrick Golden of USEPA Region 1 expressed to Maryellen Johns of WR Grace the Government Parties concern that the benthic invertebrates in the pore water of sediments need to (be) further evaluated to determine if there are any unacceptable risks(s) to the benthic invertebrate community

Transect 6 is the location where the highest concentrations of VOCs have been detected in groundwater located approximately six inches below the sediment surface water interface VOCs detected in groundwater at Transect 6 (ASBRV-T6) include 11shydichlorothene or vinylidene chloride (VDC) at concentrations up to 420 ugL and vinyl chloride at concentrations up to 100 ugL VOCs detected in groundwater samples collected beneath the Assabet River at Transects 1 through 6 (ASBRV-T1 through ASBRV-T6) are included in Table 1 and their locations are shown on Figure 1 VOCs have not been detected in surface water samples collected in the Assabet River as part of the Remedial Investigation

20 FIELD SAMPLING

Benthic invertebrate samples were collected at the following stations in the Assabet River

bull ASBRV-T3 Transect 3 a location along the WR Grace site where VDC was not detected in groundwater beneath the river

bull ASBRV-T6 Transect 6 where the highest concentration of VDC was detected in groundwater beneath the river and

bull ASBRV-UP7 a location upstream of the WR Grace site and Route 62

Figure 1 shows these locations in relationship to the Grace property

Five replicate benthic invertebrate samples per station were collected with a Surber sampler Water quality parameters of pH dissolved oxygen specific conductance and temperature (Table 2) were also measured at each station The five replicate sampling locations were selected to have as similar a benthic substrate as possible The sediment type at each location consisted of gravel and small cobbles Although there was aquatic vegetation near many of the sampling locations we tried not to sample directly in a stand

of vegetation Most of the submerged vegetation we observed was awlleaf arrowhead (Sagittaria subulata) The benthic invertebrate samples were sent to Aquatec Biological Services of Williston Vermont for identification to the lowest taxonomic level practicable (usually species or genus) and enumeration Their report is attached as Appendix A

30 DATA EVALUATION

The following metrics were calculated for the data total abundance total number of taxa dominant species fraction Shannon-Weiner Index Evenness and Ephemerotpera Plecotera Tricoptera Index The results are in Table 3

bull Abundance is a direct count of the number of individual organisms The number of individuals is a measure of the standing stock of the benthic community and provides an indication of the ability of the benthic community to serve as a prey base for higher trophic levels

bull Taxa richness is a count of the number of different taxa (in most cases taxa are counted at the level of the species) The assumption is that high diversity is indicative of a benthic community with a greater diversity of microhabitats and a broader range of species with varying tolerances to physical biological and chemical interactions

bull Dominant species fraction is the ratio of the total number of individuals from the most common species divided by the total abundance for each station As with taxa richness the assumption is that a benthic community that is not diverse and is dominated by one or more species reflects a community with a more narrow range of adaptations and tolerances to physical biological and chemical interactions Smaller values of this metric represent a more diverse benthic community

bull The Shannon-Weiner Diversity Index (H) provides another useful comparative measure of benthic community structure (Begon et al 1990) The index is species-based and assesses diversity using both the number of species (species richness) and the number of individuals (abundance) per species Although more complex statistical testing can be employed to compare H statistics generally the greater the Shannon-Weiner Diversity Index the greater the diversity of benthic species at a station

The Shannon-Weiner Index is calculated as follows

=-gt log p Where

p = frequency of species i in the sample - N N~

N = number of individuals of species i

N = total individuals in sample S = number of species

bull Evenness Index (Pielous J) provides another useful comparative measure of benthic community health as some research has indicated that this index may be the aspect of community structure most strongly affected by response to chemical contamination (Dr E Gallagher personal communication) Similar to the Shannon-Weiner Index the Evenness Index is species-based and assesses the relative abundance of individuals among the species Generally the greater the Evenness Index the more equitable the distribution of individuals among the species

The Evenness Index is calculated as follows

max

Where

H = Shannon-Weiner Diversity Index H max = log of k (number of taxonomic groups)

bull EPT INDEX

An EPT Index was calculated as the number of taxa within the orders Ephemeroptera Plecoptera and Tricoptera at each station in comparison to the upstream reference This index summarizes taxa richness within the insect orders that are considered sensitive to pollution (Plafkin et al 1989)

31 Results

Figures 2 through 6 are box and whisker plots of the metrics abundance number of taxa Shannon-Weiner Index dominant species fraction or evenness per sampling station

Abundance

As shown in Figure 2 total abundance is highest in the upstream sample while abundance at ASBRV-T3 and ASBRV-T6 are within the same range as each other

Number of Taxa

Figure 3 shows a large range in the number of taxa at ASBRV-T3 Number of taxa at ASBRV-T6 and the upstream sample fall within this range

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

1

2

3 4

5

6

TABLE OF CONTENTS

10 INTRODUCTION 1

20 FIELD SAMPLING

30 DATA EVALUATION

31 RESULTS 32 STATISTICAL EVALUATION

40 CONCLUSIONS

50 REFERENCES

10 INTRODUCTION

Menzie-Cura amp Associates Inc sampled benthic invertebrates in the Assabet River on August 4 2003 according to our letter of June 23 2003 that was approved by the Government Parties in their letter of July 31 2003 The purpose of this sampling was to evaluate whether Volatile Organic Compounds (VOCs) in groundwater discharging to the Assabet River at Transect 6 are having an adverse affect on the abundance and diversity of the population of benthic invertebrates in the river In an e-mail of March 24 2003 Derrick Golden of USEPA Region 1 expressed to Maryellen Johns of WR Grace the Government Parties concern that the benthic invertebrates in the pore water of sediments need to (be) further evaluated to determine if there are any unacceptable risks(s) to the benthic invertebrate community

Transect 6 is the location where the highest concentrations of VOCs have been detected in groundwater located approximately six inches below the sediment surface water interface VOCs detected in groundwater at Transect 6 (ASBRV-T6) include 11shydichlorothene or vinylidene chloride (VDC) at concentrations up to 420 ugL and vinyl chloride at concentrations up to 100 ugL VOCs detected in groundwater samples collected beneath the Assabet River at Transects 1 through 6 (ASBRV-T1 through ASBRV-T6) are included in Table 1 and their locations are shown on Figure 1 VOCs have not been detected in surface water samples collected in the Assabet River as part of the Remedial Investigation

20 FIELD SAMPLING

Benthic invertebrate samples were collected at the following stations in the Assabet River

bull ASBRV-T3 Transect 3 a location along the WR Grace site where VDC was not detected in groundwater beneath the river

bull ASBRV-T6 Transect 6 where the highest concentration of VDC was detected in groundwater beneath the river and

bull ASBRV-UP7 a location upstream of the WR Grace site and Route 62

Figure 1 shows these locations in relationship to the Grace property

Five replicate benthic invertebrate samples per station were collected with a Surber sampler Water quality parameters of pH dissolved oxygen specific conductance and temperature (Table 2) were also measured at each station The five replicate sampling locations were selected to have as similar a benthic substrate as possible The sediment type at each location consisted of gravel and small cobbles Although there was aquatic vegetation near many of the sampling locations we tried not to sample directly in a stand

of vegetation Most of the submerged vegetation we observed was awlleaf arrowhead (Sagittaria subulata) The benthic invertebrate samples were sent to Aquatec Biological Services of Williston Vermont for identification to the lowest taxonomic level practicable (usually species or genus) and enumeration Their report is attached as Appendix A

30 DATA EVALUATION

The following metrics were calculated for the data total abundance total number of taxa dominant species fraction Shannon-Weiner Index Evenness and Ephemerotpera Plecotera Tricoptera Index The results are in Table 3

bull Abundance is a direct count of the number of individual organisms The number of individuals is a measure of the standing stock of the benthic community and provides an indication of the ability of the benthic community to serve as a prey base for higher trophic levels

bull Taxa richness is a count of the number of different taxa (in most cases taxa are counted at the level of the species) The assumption is that high diversity is indicative of a benthic community with a greater diversity of microhabitats and a broader range of species with varying tolerances to physical biological and chemical interactions

bull Dominant species fraction is the ratio of the total number of individuals from the most common species divided by the total abundance for each station As with taxa richness the assumption is that a benthic community that is not diverse and is dominated by one or more species reflects a community with a more narrow range of adaptations and tolerances to physical biological and chemical interactions Smaller values of this metric represent a more diverse benthic community

bull The Shannon-Weiner Diversity Index (H) provides another useful comparative measure of benthic community structure (Begon et al 1990) The index is species-based and assesses diversity using both the number of species (species richness) and the number of individuals (abundance) per species Although more complex statistical testing can be employed to compare H statistics generally the greater the Shannon-Weiner Diversity Index the greater the diversity of benthic species at a station

The Shannon-Weiner Index is calculated as follows

=-gt log p Where

p = frequency of species i in the sample - N N~

N = number of individuals of species i

N = total individuals in sample S = number of species

bull Evenness Index (Pielous J) provides another useful comparative measure of benthic community health as some research has indicated that this index may be the aspect of community structure most strongly affected by response to chemical contamination (Dr E Gallagher personal communication) Similar to the Shannon-Weiner Index the Evenness Index is species-based and assesses the relative abundance of individuals among the species Generally the greater the Evenness Index the more equitable the distribution of individuals among the species

The Evenness Index is calculated as follows

max

Where

H = Shannon-Weiner Diversity Index H max = log of k (number of taxonomic groups)

bull EPT INDEX

An EPT Index was calculated as the number of taxa within the orders Ephemeroptera Plecoptera and Tricoptera at each station in comparison to the upstream reference This index summarizes taxa richness within the insect orders that are considered sensitive to pollution (Plafkin et al 1989)

31 Results

Figures 2 through 6 are box and whisker plots of the metrics abundance number of taxa Shannon-Weiner Index dominant species fraction or evenness per sampling station

Abundance

As shown in Figure 2 total abundance is highest in the upstream sample while abundance at ASBRV-T3 and ASBRV-T6 are within the same range as each other

Number of Taxa

Figure 3 shows a large range in the number of taxa at ASBRV-T3 Number of taxa at ASBRV-T6 and the upstream sample fall within this range

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

10 INTRODUCTION

Menzie-Cura amp Associates Inc sampled benthic invertebrates in the Assabet River on August 4 2003 according to our letter of June 23 2003 that was approved by the Government Parties in their letter of July 31 2003 The purpose of this sampling was to evaluate whether Volatile Organic Compounds (VOCs) in groundwater discharging to the Assabet River at Transect 6 are having an adverse affect on the abundance and diversity of the population of benthic invertebrates in the river In an e-mail of March 24 2003 Derrick Golden of USEPA Region 1 expressed to Maryellen Johns of WR Grace the Government Parties concern that the benthic invertebrates in the pore water of sediments need to (be) further evaluated to determine if there are any unacceptable risks(s) to the benthic invertebrate community

Transect 6 is the location where the highest concentrations of VOCs have been detected in groundwater located approximately six inches below the sediment surface water interface VOCs detected in groundwater at Transect 6 (ASBRV-T6) include 11shydichlorothene or vinylidene chloride (VDC) at concentrations up to 420 ugL and vinyl chloride at concentrations up to 100 ugL VOCs detected in groundwater samples collected beneath the Assabet River at Transects 1 through 6 (ASBRV-T1 through ASBRV-T6) are included in Table 1 and their locations are shown on Figure 1 VOCs have not been detected in surface water samples collected in the Assabet River as part of the Remedial Investigation

20 FIELD SAMPLING

Benthic invertebrate samples were collected at the following stations in the Assabet River

bull ASBRV-T3 Transect 3 a location along the WR Grace site where VDC was not detected in groundwater beneath the river

bull ASBRV-T6 Transect 6 where the highest concentration of VDC was detected in groundwater beneath the river and

bull ASBRV-UP7 a location upstream of the WR Grace site and Route 62

Figure 1 shows these locations in relationship to the Grace property

Five replicate benthic invertebrate samples per station were collected with a Surber sampler Water quality parameters of pH dissolved oxygen specific conductance and temperature (Table 2) were also measured at each station The five replicate sampling locations were selected to have as similar a benthic substrate as possible The sediment type at each location consisted of gravel and small cobbles Although there was aquatic vegetation near many of the sampling locations we tried not to sample directly in a stand

of vegetation Most of the submerged vegetation we observed was awlleaf arrowhead (Sagittaria subulata) The benthic invertebrate samples were sent to Aquatec Biological Services of Williston Vermont for identification to the lowest taxonomic level practicable (usually species or genus) and enumeration Their report is attached as Appendix A

30 DATA EVALUATION

The following metrics were calculated for the data total abundance total number of taxa dominant species fraction Shannon-Weiner Index Evenness and Ephemerotpera Plecotera Tricoptera Index The results are in Table 3

bull Abundance is a direct count of the number of individual organisms The number of individuals is a measure of the standing stock of the benthic community and provides an indication of the ability of the benthic community to serve as a prey base for higher trophic levels

bull Taxa richness is a count of the number of different taxa (in most cases taxa are counted at the level of the species) The assumption is that high diversity is indicative of a benthic community with a greater diversity of microhabitats and a broader range of species with varying tolerances to physical biological and chemical interactions

bull Dominant species fraction is the ratio of the total number of individuals from the most common species divided by the total abundance for each station As with taxa richness the assumption is that a benthic community that is not diverse and is dominated by one or more species reflects a community with a more narrow range of adaptations and tolerances to physical biological and chemical interactions Smaller values of this metric represent a more diverse benthic community

bull The Shannon-Weiner Diversity Index (H) provides another useful comparative measure of benthic community structure (Begon et al 1990) The index is species-based and assesses diversity using both the number of species (species richness) and the number of individuals (abundance) per species Although more complex statistical testing can be employed to compare H statistics generally the greater the Shannon-Weiner Diversity Index the greater the diversity of benthic species at a station

The Shannon-Weiner Index is calculated as follows

=-gt log p Where

p = frequency of species i in the sample - N N~

N = number of individuals of species i

N = total individuals in sample S = number of species

bull Evenness Index (Pielous J) provides another useful comparative measure of benthic community health as some research has indicated that this index may be the aspect of community structure most strongly affected by response to chemical contamination (Dr E Gallagher personal communication) Similar to the Shannon-Weiner Index the Evenness Index is species-based and assesses the relative abundance of individuals among the species Generally the greater the Evenness Index the more equitable the distribution of individuals among the species

The Evenness Index is calculated as follows

max

Where

H = Shannon-Weiner Diversity Index H max = log of k (number of taxonomic groups)

bull EPT INDEX

An EPT Index was calculated as the number of taxa within the orders Ephemeroptera Plecoptera and Tricoptera at each station in comparison to the upstream reference This index summarizes taxa richness within the insect orders that are considered sensitive to pollution (Plafkin et al 1989)

31 Results

Figures 2 through 6 are box and whisker plots of the metrics abundance number of taxa Shannon-Weiner Index dominant species fraction or evenness per sampling station

Abundance

As shown in Figure 2 total abundance is highest in the upstream sample while abundance at ASBRV-T3 and ASBRV-T6 are within the same range as each other

Number of Taxa

Figure 3 shows a large range in the number of taxa at ASBRV-T3 Number of taxa at ASBRV-T6 and the upstream sample fall within this range

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

of vegetation Most of the submerged vegetation we observed was awlleaf arrowhead (Sagittaria subulata) The benthic invertebrate samples were sent to Aquatec Biological Services of Williston Vermont for identification to the lowest taxonomic level practicable (usually species or genus) and enumeration Their report is attached as Appendix A

30 DATA EVALUATION

The following metrics were calculated for the data total abundance total number of taxa dominant species fraction Shannon-Weiner Index Evenness and Ephemerotpera Plecotera Tricoptera Index The results are in Table 3

bull Abundance is a direct count of the number of individual organisms The number of individuals is a measure of the standing stock of the benthic community and provides an indication of the ability of the benthic community to serve as a prey base for higher trophic levels

bull Taxa richness is a count of the number of different taxa (in most cases taxa are counted at the level of the species) The assumption is that high diversity is indicative of a benthic community with a greater diversity of microhabitats and a broader range of species with varying tolerances to physical biological and chemical interactions

bull Dominant species fraction is the ratio of the total number of individuals from the most common species divided by the total abundance for each station As with taxa richness the assumption is that a benthic community that is not diverse and is dominated by one or more species reflects a community with a more narrow range of adaptations and tolerances to physical biological and chemical interactions Smaller values of this metric represent a more diverse benthic community

bull The Shannon-Weiner Diversity Index (H) provides another useful comparative measure of benthic community structure (Begon et al 1990) The index is species-based and assesses diversity using both the number of species (species richness) and the number of individuals (abundance) per species Although more complex statistical testing can be employed to compare H statistics generally the greater the Shannon-Weiner Diversity Index the greater the diversity of benthic species at a station

The Shannon-Weiner Index is calculated as follows

=-gt log p Where

p = frequency of species i in the sample - N N~

N = number of individuals of species i

N = total individuals in sample S = number of species

bull Evenness Index (Pielous J) provides another useful comparative measure of benthic community health as some research has indicated that this index may be the aspect of community structure most strongly affected by response to chemical contamination (Dr E Gallagher personal communication) Similar to the Shannon-Weiner Index the Evenness Index is species-based and assesses the relative abundance of individuals among the species Generally the greater the Evenness Index the more equitable the distribution of individuals among the species

The Evenness Index is calculated as follows

max

Where

H = Shannon-Weiner Diversity Index H max = log of k (number of taxonomic groups)

bull EPT INDEX

An EPT Index was calculated as the number of taxa within the orders Ephemeroptera Plecoptera and Tricoptera at each station in comparison to the upstream reference This index summarizes taxa richness within the insect orders that are considered sensitive to pollution (Plafkin et al 1989)

31 Results

Figures 2 through 6 are box and whisker plots of the metrics abundance number of taxa Shannon-Weiner Index dominant species fraction or evenness per sampling station

Abundance

As shown in Figure 2 total abundance is highest in the upstream sample while abundance at ASBRV-T3 and ASBRV-T6 are within the same range as each other

Number of Taxa

Figure 3 shows a large range in the number of taxa at ASBRV-T3 Number of taxa at ASBRV-T6 and the upstream sample fall within this range

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

N = total individuals in sample S = number of species

bull Evenness Index (Pielous J) provides another useful comparative measure of benthic community health as some research has indicated that this index may be the aspect of community structure most strongly affected by response to chemical contamination (Dr E Gallagher personal communication) Similar to the Shannon-Weiner Index the Evenness Index is species-based and assesses the relative abundance of individuals among the species Generally the greater the Evenness Index the more equitable the distribution of individuals among the species

The Evenness Index is calculated as follows

max

Where

H = Shannon-Weiner Diversity Index H max = log of k (number of taxonomic groups)

bull EPT INDEX

An EPT Index was calculated as the number of taxa within the orders Ephemeroptera Plecoptera and Tricoptera at each station in comparison to the upstream reference This index summarizes taxa richness within the insect orders that are considered sensitive to pollution (Plafkin et al 1989)

31 Results

Figures 2 through 6 are box and whisker plots of the metrics abundance number of taxa Shannon-Weiner Index dominant species fraction or evenness per sampling station

Abundance

As shown in Figure 2 total abundance is highest in the upstream sample while abundance at ASBRV-T3 and ASBRV-T6 are within the same range as each other

Number of Taxa

Figure 3 shows a large range in the number of taxa at ASBRV-T3 Number of taxa at ASBRV-T6 and the upstream sample fall within this range

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Dominant Species Fraction

The range of this metric is similar among the three sampling locations indicating a similar benthic community diversity among stations

Shannon-Weiner Index

The range of the data is greatest at ASBRV-T-3 The range of the index values at the other two stations falls within the range at ASBRV-T-3

Evenness

Similar to the Shannon-Weiner Index and number of taxa the range of values for this metric is greatest at ASBRV-T-3 and the values at the other two stations fall within this range

EPT Index

The EPT values for ASBRV-T-3 and ASBRV-T-6 are 100 and 75 respectively of the upstream station (ASBRV-UP-7) This indicates that these sensitive species are as well represented at ASBRV-T-3 as they are at the upstream station There is a decrease in the sensitive EPT species at ASBRV-T-6

32 Statistical Evaluation

To evaluate whether any of these potential trends are statistically significant we used an analysis of variance (ANOVA) to test the null hypothesis H0 that there are no differences among sampling locations for abundance number of taxa Shannon-Wiener Index dominant species fraction or evenness Because only one EPT index was calculated per station we did not evaluate this metric statistically Results of statistical tests conducted using Statistica software are presented in Appendix B

We believe that the underlying assumptions of normality and homogeneity of variance are met for use of an ANOVA because the data are normally distributed (Figures 7 through 11) and the Levene test for homogeneity of variance indicates that each parameter with the exception of total number of taxa have equal variances Therefore the underlying assumptions for the use of an ANOVA are met and an ANOVA is an appropriate test for the parameters with the possible exception of number of taxa However according to Zar (1974) the ANOVA is robust even when there is considerable heterogeneity of variance as long as all n are equal (ie that the number of replicates is the same among treatments or stations) as they are for these samples

The results indicate a statistically significant difference for total abundance at a significance level of 5 but no statistical difference for the other parameters Therefore there is a statistically significant difference among the three stations for total abundance

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

There is not a statistically significant difference among the three stations for number of taxa Shannon-Weiner Index dominant species fraction or evenness

We also employed a non-parametric ANOVA on the number of taxa This is a more appropriate test for use when the underlying assumptions of the parametric ANOVA are not met but it has less power It also indicated there were no significant differences among stations in the number of taxa

For total abundance we employed multiple comparison tests to evaluate which stations were responsible for the significant difference among stations for this parameter Tukeys test indicated no significant difference between ASBRV-T-6 and ASBRV-T-3 but statistical differences between ASBRV-T-6 and ASBRV-UP-7 and ASBRV-T-3 and ASBRV-UP-7

We also compared the data at ASBRV-T-3 and ASBRV-T-6 to the upstream station through the use of Dunnetts test which compares each station to the control It also indicated that the total abundance at ASBRV-T-3 and ASBRV-T-6 are statistically different from the total abundance at ASBRV-UP-7

40 CONCLUSIONS

A statistical analysis of the benthic invertebrate data collected from the Assabet River indicates that the total abundance or total number of organisms per sample is different at the upstream station ASBRV-UP-7 than at either of the downstream samples No statistically significant differences were identified among stations for number of taxa or for measures of community diversity such as dominant species fraction Shannon-Weiner index or evenness There was no statistical difference in abundance between ASBRV-Tshy6 and ASBRV-T-3 The EPT index measuring the presence of sensitive species indicated that ASBRV-T-3 was similar to upstream but some decrease in sensitive species was present at ASBRV-T-6 There appears to be a lower number of organisms at ASBRV-T-3 and ASBRV-T6 along the site compared to upstream The sampling station ASBRV-T6 was located where the highest VDC concentrations were detected in groundwater discharging to the Assabet River however no VDC was detected at sampling station ASBRV-T-3 Therefore although there is some observed decrease in the number of organisms the cause of the decrease is inconclusive because it is present both at ASBRV-T-3 and ASBRV-T-6 It is possible that the VDC is having an effect on more sensitive species at ASBRV-T-6 However this decrease in sensitive species affects a small area and does not appear to have an overall effect on the presence or diversity of benthic invertebrates in the 3200 feet of the Assabet River bordering the WR Grace Site

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

50 REFERENCES

Begon M JL Harper and CR Townshend 1990 Ecology Individuals Populations and Communities Blackwell Scientific Publications Boston

Plafkin JL MT Barbour KD Porter SK Gross and R M Hughes 1989 Rapid Bioassessment Protocols for use in Streams and Rivers Benthic Macroinvertebrates and Fish US Environmental Protection Agency Assessment and Watershed Protection Division EPA 4404-89-001

Zar J 1974 Biostatistical Analysis Prentice Hall Inc Englewood Cliffs NJ

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

TABLES

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

HOJL BASE UAP BY COL-EAST 1998 WELL LOCATIONS WR GRACE PROPERTY LME ft MISC SITE FEATURES COMPILED FROM MFORUATION PROVIDED BY CAMP DRESSER ft IfcKEE SURROUNDMG PROPERTY LME LOCATIONS ARE FROM THE TOWN OF ACTON AND CONCORD ASSESSORS MAPS

SURFACE WATER ft SEDIMENT SAMPLE LOCATION

09 SEQUENT SAMPLE LOCATION

laquoR GRACE PROPERTY BOUNDARY

MONITORING WELL (SMGLE OR CLUSTER)

bull EXTRACTION WELL

e PUBLIC WATER SUPPLY WELL

bull PROPOSED PUBLIC WATER SUPPLY WELL

TRANSECT

PEZOUETER

Originals in ootoi

Assabet River Benthic Invertebrate Sampling Locations

WR Grace Acton MA

CHECKED FIGURE

DRAFTED RMK

FILE 1GeoTiK HIM mm oomwr M J

DATE 102403

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

8

600

500

400

robull

300

To o

200

100

Figure 2 Box and Whisker Plot Assabet River Benthic Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Total Abundance

a Median ASBRV-TB-3 ASBRV-UPB-7 I I 25-75

ASBRV-TB-6 Min-Max Sample ID

Total abundance is the number of organisms per sample Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Figure 3 Box and Whisker Plot Total Number of Taxa Assabet River Benthic Cata WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot No of Taxa 38

36

34

TO X TO

28

22

20

18 ASBRV-TB-3 ASBRV-UPB-7

ASBRV-TB-6

Sample ID

Median 25-75 Min-Max

Total number of taxa is the number of taxa (typically to species) per sample Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

050

045

040

g

2 035

sect_ 030 C3

I 025

o o

020

015

010

Figure 4 Box and Whisker Plot Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Dominant Species Fraction

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

a Median I I 25-75 ~T Min-Max

Dominant species fraction is the ration of the number of organisms from the most numerous species to total abundance per station Lower numbers indicate a more diverse population Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Figure 5 Box and Whisker Plot Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

135 Categ Box amp Whisker Plot SW Index

130

125

120

0)

c 115

110

105

100

095

090 ASBRV-TB-3

ASBRV-TB-6

Sample ID

ASBRV-UPB-7 n Median HI 25-75

Min-Max

Shannon-Weiner Index is a measure of benthic community diversity The higher the value the greater the diversity per station Note that field designation ASBRV-TB-3 is equivalent to sampling station ASBRV-T-3 etc

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

100

095

090

085

CO (0

I 080

UJ

075

070

065

060

Figure 6 Box and Whisker Plot Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Categ Box amp Whisker Plot Eveness

ASBRV-TB-3 ASBRV-UPB-7 ASBRV-TB-6

Sample ID

D Median 25-75 Min-Max

Evenness is a measure of the distribution of individuals among species The higher the value the greater the community diversity Note the field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

o R 3

Figure 7 Total Abundance Histogram Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Total Abundance K-S d= 17295 pgt 20 Lilliefors pgt 20

mdash Expected Normal

-100 100 200 300 400 500

X lt= Category Boundary

600

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Figure 8 Histogram for Number of Taxa Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram No of Taxa K-S d= 14962 pgt 20 Lilliefors pgt 20

mdash Expected Normal

20 25 30 35 40

X lt= Category Boundary

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Figure 9 Histogram for Dominant Species Fraction Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Dominant Species Fraction K-S d= 15505 pgt 20 Lilliefors pgt 20

mdash Expected Normal

00 01 02 03 05

X lt= Category Boundary

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Figure 10 Histogram for Shannon-Weiner Index Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram SW Index K-S d=11203 pgt 20 Lilliefors pgt 20

mdash Expected Normal

10 11 12 13 14

X lt= Category Boundary

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

6

5

o o 4 6 z

3

2

Figure 11 Histogram for Evenness Assabet River Benthic Invertebrate Data WR Grace Site Acton Massachusetts

Histogram Eveness K-S d=13185 pgt 20 Lilliefors pgt 20

mdash Expected Normal

05 06 07 08 09

X lt= Category Boundary

10

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

APPENDIX A

MACROINVERTEBRATE DATA REPORT

FROM AQUATEC BIOLOGICAL SCIENCES

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Aquatec Biological Sciences Environmental Vi I Natural Resource

Ecofcgy ^ ^SSST ^ EE^ST IS bull-laquo

Sciences

August 29 2003

Ms Katherine Fogerty Menzie-Cura amp Associates One Courthouse Lane Suite 2 Chelmsford MA01824

SB

Dear Ms Fogerty

Attached please find the report on the macroinvertebrate samples received for the WR Grace Assabet River The goal for processing the samples was to obtain a minimum of 300 organisms per sample In most samples the entire sample was processed and less than 300 organisms were obtained

This submittal completes the macroinvertebrate analyses for this project

If you have any questions or we can be of further assistance please dont hesitate to contact me

Sincerely

Philip C Downey PhD CFS Director

273 Commerce Street Williston VT 05495 Tel 8028601638 Fax 8026583189

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Note that this report uses the field designations such as ASBRV-TB-3-1 This represents sample location ASBRV-T-3 replicate 1

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Aquatec Biological Sciences Sciences

Environmental Toxicology

VraquoIbull Jr

Natural Resource Assessments

Jamp mt

Microbiology

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA 01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24987 DateTime Sample Collected 8403 110000 AM

lent Sample ID ASBRV-TB-3-1 Percent Sample Examined 100

marks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

ltelminthes Turbellana Tndadida Plananidae Dugesia tignna 10

emertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 3

ematoda Dorytaimida Alaimus sp 1

annelida Oligochaeta Lumbncina Lumbncidae 1

Tubificida Tubificidae Limnodnlus claparedtanus 1

Molluscs Gastropoda Basommatophora Ancylidae Laevapex fuscus 6

Planorbidae Gyrauus deflectus 1

Menetus dilatus 10

Planorbella tnvohns 1

Mesogastropoda Hydrobndae Ammcola limosa 31

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

Sphaenum sp 11

rthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 3

Insects Diptera Chironomidae Chironominae Chironommi Dicrotendipes neomodestus 6

Mtcrotendipes caelum 2

Polypedilum convictum 14

Polypedilum illtnoense 2

Tanytarsim Rheotanylarsus distmctissimus 2

Rheotanytarsus exiguus 31

Orthocladnnae Orthocladimi Cncotopus bictnctus 5

ABS Page 1 of 30

273 Commerce Street Williston VT 05495 Teh 802860 1638 Fax- 8026583189

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24987

ASBRV-TB-3-1

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

00AM

Phylum

Arthropoda

Class

Insecta

Order

Diptera

Family

Chironomidae

Sub-Family

Orthocladiinae

Tribe

Orthocladimi

GenusSpeciesVariety

Cncotopus tnfascia

Counted

2

Nanocladius crassicomus 1

Synorthodadius semrvirens 3

Tvetema discolonpes 9

Tanypodinae tarsa sp 4

Pentaneunni Ablabesmyia mallochi 2

Tipulidae Antodia sp 1

Ephemeroptera Baetidae Acentretla sp 2

Tncorythidae Tncorythodes sp 9

Odonata Coenagnonidae Enallagma sp 1

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 24

Hydropsyche bettem 9

Hydroptilidae Hydroptilinae Hydroptila sp 16

Leptocendae Nectopsyche sp 4

Philopotamidae Chimarra obscura 2

Sub-Total 233

Grand Total 233

ABS Page 2 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

ooratory Sample ID

i ent Sample ID

Remarks

24988

ASBRV-TB-3-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100 Not Reported

iylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmtties Turbellana Tncladida Plananidae Dugesia tignna 1

nemertea Enopla Hoplonemertea Prostomatidae Prasloma graecense 2

Oligochaeta Lumbncina Lumbnadae 2

jllusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Planorbidae Gyraulus deflectus 1

Menetus dilatus 1

Mesogastropoda Hydrobiidae Ammcola limosa 81

Petecypoda Pnonodesmacea Sphaenidae Pisidium sp 4

Sphaenum sp 1

hropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 16

Insects Coleoptera Elmidae Stenelmis sp 1

Diptera Ceratopogomdae Sezaa sp 1

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 1

Microtendipes caelum 6

Parachironomus sp 1

Polypedilum convtctum 3

Polypedilum illinoense 3

Polypedilum tfigonus 1

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 16

Orthocladimae Corynoneunni Thienemanmella xena 4

ABS Page 3 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Laboratory Sample ID 24988 DateTime Sample Collected 8403 110000 AM Client Sample ID ASBRV-TB-3-2 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Arthropoda Insecta Diptera Chironomidae Orthocladiinae Orthocladiini Cricotopus bicinctus 1

Synorthodadius semivirens 1

Tanypodinae Larsia sp 3

Pentaneurini Ablabesmyia mallochi 1

Ephemeroptera Baetidae Acentrella sp 2

Tricorythidae Tricorythodes sp 1

Trichoptera Hydropsychidae Cheumatopsyche sp 5

Hydropsyche betteni 3

Symphitopsyche spama 1

Leptoceridae Mystacides sp 1

Nectopsyche sp 1

Sub-Total 168

Grand Total 168

ABS Page 4 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

i ooratory Sample ID 24989 DateTime Sample Collected 8403 110000 AM

ent Sample ID ASBRV-TB-3-3 Percent Sample Examined 100

Remarks Vegetation detritus Sampling Depth (m) Not Reported

lytum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes Turbellana Tncladida Plananidae Dugesia tignna 1

ineltda Oligochaeta Tubificida Naididae Nais sp 1

Mollusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 2

Mesogastropoda Hydrobiidae Amnicola limosa 2

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insects Diptera Chironomidae Chironommae Chironomini Microtendipes caeum 1

Polypedilum conviction 1

Polypedilum illmoense 2

Tanytarsini Rheotanytarsus exiguus 3

Orthocladunae Limnophyes sp 1

Corynoneunni Thienemanmella xena 1

Orthocladnni Cncotopus bmnctus 2

Tvetenia discolonpes 1

Tipulidae Antocha sp 1

Ephemeroptera Baetidae Acentrella sp 1

Tncorythidae Tncorythodes sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 2

Symphrtopsyche spama 1

Psychomyiidae Psychomyia sp 1

Sub-Total 26

ABS Page 5 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample IDClient Sample IDRemarks

24989 ASBRV-TB-3-3 Vegetation detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100 Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

Grand Total 26

ABS Page 6 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

atory Sample ID 24990 DateTime Sample Collected 8403 110000 AM

- Sample ID ASBRV-TB-3-4 Percent Sample Examined 100

Remarks Vegetation pebbles detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelminthes Turbellana Tndadida Plananidae Dugesia tignna 4

Nemertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

jmatoda Dorylaimida Alaimus sp 2

ollusca Gastropoda Basommatophora Planorbidae Gyrauus deflectus 1

Menetus dilatus e

Mesogastropoda Hydrobndae Amnicola limosa 41

Pelecypoda Pnonodesmacea Sphaenidae Pisidiumsp 8

Sphaenum sp 3

Arthropoda Arachnoidea Acanformes Lebertndae Lebertjasp 1

Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 5

Microtendipes caelum 2

Polypedilum convtctum 2

Tanytarsmi Paratanytarsus sp 1

Rheotanytarsus exiguus 6

Tanytarsus sp 3

Orthocladunae Orthocladiim Cncotopus bidnctus 5

Ephemeroptera Tncorythidae Tncorythodes sp 11

Tnchoptera Brachycentndae Micrasema sp 1

Hydropsychidae Cheumatopsyche sp 3

ABS Page 7 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine FogartyMenzie Cura amp Associates1 Courthouse LaneSuite 2Chelmsford MA01824Reference WR Grace Assabet River

Date 82903 BTR No 07238

Project No 03054 No of Samples 15

Date Received 8603

Laboratory Sample ID

Silent Sample ID

Remarks

24990

ASBRV-TB-3-4

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum

Arthropoda

Class

Insects

Order

Trichoptera

Family

Hydropsychidae

Sub-Family Tribe GenusSpeciesVariety

Hydropsyche better)

Counted

1

Leploceridae Nectopsyche sp 9

Oecetis sp 1

Psychomyiidae Psychomyia sp 3

Sub-Total 124

Grand Total 124

ABS Page 8 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

_

-

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane

Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

boratory Sample ID

ent Sample ID

Remarks

24991

ASBRV-TB-3-5

Vegetation pebbles detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 1100

100

Not Reported

OOAM

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

atyhelmmthes

nelida

Turbellana

Oligochaeta

Tndadida

Lumbncina

Plananidae

Lumbncidae

Dugesia tignna 3

2

Mollusca Gastropoda

Pelecypoda

Basommatophora

Mesogastropoda

Pnonodesmacea

Ancylidae

Hydrobudae

Sphaemdae

Laevapex fuscus

Ammcola limosa

Pisidium sp

Sphaenum sp

4

18

7

1

Arthropoda Crustacea Amphipoda Gammandae Gammarus fasciatus 2

mdash

Insecta Coleoptera

Diptera

Elmidae

Chironomidae Chironominae Chironommi

Dubiraphta sp

Dicrotendipes neomodestus

Microtendipes caeum

1

2

5

_

Orthocladunae

Tanytarsmi

Corynoneunni

Rheotanytarsus exiguus

Thienemanmetla xena

9

1

Orthocladuni Cncotopus bicinctus 4

Ephemeroptera

Tnchoptera

Tipulidae

Tncorythidae

Hydropsychidae

Antocha sp

Tricorythodes sp

Hydropsyche betteni

1

2

1

Hydroptihdae Hydroptilinae Hydroptila sp 1

Leptocendae

Psychomyiidae

Nectopsyche sp

Psychomyia sp

2

1

Sub-Total 67

ABS Page 9 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fog arty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24991

ASBRV-TB-3-5 Vegetation pebbles detritus

DateTime Sample Collected Percent Sample Examined Sampling Depth (m)

8403 110000 AM

100

Not Reported

Phylum Class Order Family Sub-Family Tribe GenusSpeciesVariety

Counted

Grand Total 67

ABS Page 10 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

ratory Sample ID 24992 DateTime Sample Collected 8403 123000 PM bample ID ASBRV-TB-6-1 Percent Sample Examined 100

(emarks Vegetation detritus Sampling Depth (m) Not Reported

1 lum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

P-tyhelminlhes Turbellana Tncladida Planamdae Dugesia bgnna 1

lelida Oligochaeta Tubifiada Naididae Dero vaga 1

Ophidonais serpentine 1

gt lusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 1

Mesogastropoda Hydrobudae Ammcola limosa 16

Pelecypoda Pnonodesmacea Sphaenidae Pisidium sp 2

mdash Sphaenum sp 1

f ropoda Crustacea Amphipoda Gammandae Gammanis fasciatus 1

Insecta Coleoptera Elmidae Dubiraphia sp 1

Diptera Chironomidae Chironominae Chironomini Polypedilum illmoense 1

Tanytarsim Rheotanytarsus exiguus 5

Tanytarsus sp 1

Orthocladunae Corynoneunni Thtenemanmella xena 1

Orthocladimi Cncotopus bicinctus 1

Tanypodmae Larsia sp 1

Tipulidae Antocha sp 2

Ephemeroptera Baetidae Acentrella sp 1

Tnchoptera Hydropsychidae Cheumatopsyche sp 4

Hydropsyche better 24

Leptocendae Oecetis sp 2

bmnephilidae Neophylax sp 2

ABS Page 11 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Laboratory Sample ID

Client Sample ID

Remarks

24992 ASBRV-TB-6-1

Vegetation detritus

Phylum

Arthropoda

Class

Insecta

Order

Tnchoptera

Family

Philopotamidae

Sub-Family

Psychomyiidae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Datemme Sample Collected 8403 123000 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

ff Tribe GenusSpeciesVariety

Chimarra obscura

Counted

Psychomyia sp

Sub-Total

Grand Total

72

72

ABS Page 12 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

boratory Sample ID 24993

lent Sample ID ASBRV-TB-6-2

Remarks Vegetation sand detritus

lylum Class Order

atyhelmmthes Turbellana Tncladida

amatoda Dorylaimida

Molluscs Gastropoda Basommatophora

Mesogastropoda

Pelecypoda Pnonodesmacea

bullnropoda Crustacea Amphipoda

Insecta Diptera

Tnchoptera

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 1230 00 PM

Percent Sample Examined 100 Sampling Depth (m) Not Reported

Family Sub-Family Tribe GenusSpeciesVariety Counted

Plananidae Dugesia tignna 1

Alaimus sp 1

Planorbidae Menetus dilatus 2

Hydrobndae Amnicola hmosa 10

Sphaenidae Pisidium sp 3

Sphaenum sp 2

Unionidae Elliptic complanata 1

Gammandae Gammarus fasciatus e

Chironomidae Chironommae Chironomini Dicrotendipes neomodestus 3

Polypedilum illinoense 1

Tanytarsmi Rheotanytarsus distmctissimus 8

Rheotanytarsus exiguus 7

Orthocladiinae Orthocladiini Cncotopus bicinctus 1

Nanocladius crassicomus 1

Synorthocladius semvirens 1

Tvetenta dtscolonpes 1

Tanypodinae Larsta sp 1

Tipuhdae Antocha sp 2

Hydropsychidae Cheumatopsyche sp 4

Hydropsyche betteru 7

Hydroptilidae Hydroptilmae Hydroptila sp 1

ABS Page 13 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

24993

ASBRV-TB-6-2

Vegetation sand detritus

DateTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 123000 PM

100

Not Reported

Phylum

Arthropods

Class

Insecta

Order

Tnchoptera

Family

Leptocendae

Sub-Family Tribe GenusSpeciesVariety

Tnaenodes sp

ff Counted

Psychomyiidae Psychomyia sp

Sub-Total 70

Grand Total 70

ABS Page 14 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Date 82903 Menzie Cura amp Associates BTR No 07238 1 Courthouse Lane Project No 03054 Suite 2 No of Samples 15 Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

Moratory Sample ID 24994 DateTime Sample Collected 8403 123000 PM

nt Sample ID ASBRV-TB-6-3 Percent Sample Examined 100

Remarks Vegetation sand detritus Sampling Depth (m) Not Reported

i ylum Clasi Order Family Sub-Family Tribe GenusfSpeclesVarlety Counted

N^mertea Ertopla Hoplonemertea Prostomalidae Prostoma graecense 1

lelida Oligochaeta Tubrfictda Naididae Dero vaga 1

Tubificidae Lmnodntus claparedianus 2

1 llusca Gastropoda Basommatophora Ancylidae Laevapex fuscus 3

Mesogastropoda Hydrobndae Ammcola limosa 4

Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 3

mdash Sphaenum sp 1

iropoda Arachnoidea Acanformes Sperchomdae Sperchon sp 1

mdash Crustacea Amphipoda Gammandae Gammarus fasciatus 1

Insecta Diptera Chironomidae Chironommae Chironommi Dicrotendipes neomodestus 1

Mcrotendipes caeum 1

Polypedtlum convtctum 2

Polypedilum illinoense 7

Tanytarsini Rheotanytarsus disttnctissimus 5

Rheotanytarsus exiguus 11

Orthocladnnae Orthocladnni Tvetema discolonpes 3

Simulndae Simulium sp 1

Tipulidae Antocha sp 3

Ephemeroptera Baetidae Acentrella sp 2

Tncorythidae Tncorythodes sp 2

Odonata Coenagnomdae Enallagma sp 1

ABS Page 15 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA 01824 Reference WR Grace Assabet River

aboratory Sample ID 24994

iient Sample ID ASBRV-TB-6-3 Remarks Vegetation sand detritus

Phylum Class Order

Arthropoda Insects Tnchoptera

Family

Hydropsychidae

Sub-Family

Leplocendae

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

DateTime Sample Collected 8403 123000 PM Percent Sample Examined 100 Sampling Depth (m) Not Reported

Tribe GenusSpeciesVariety

Cheumatopsyche sp

Counted

Hydropsyche betten

SympMopsyche spama

Nectopsyche sp

Sub-Total

Grand Total

109

109

ABS Page 16 of 30

Katherine Fogarty Date 82903 ~ Menzie Cura amp Associates BTR No 07238

1 Courthouse Lane Project No 03054 _ Suite 2 No of Samples 15

Chelmsford MA01824 Date Received 8603 Reference WR Grace Assabet River

mdash Laboratory Sample ID 24995 DateTime Sample Collected 8403 1230 OOPM

ent Sample ID ASBRV-TB-6-4 Percent Sample Examined 100 ftvmarks Vegetation sand detritus Sampling Depth (m) Not Reported

lylum Class Order Family Sub-Family Tribe GenusSpeciesVariety Counted

rlatyhelmmthes Turbellana Tncladida Plananidae Dugesia tigrina 2

imertea Enopla Hoplonemertea Prostomatidae Prostoma graecense 1

Mollusca Gastropoda Basommatophora Planorbidae Menetus dilatus 1

Planorbella tnvolvis 1i laquobull

Mesogastropoda Hydrobiidae Ammcola limosa 10

mdash Pelecypoda Pnonodesmacea Sphaemdae Pisidium sp 2

W Sphaenum sp 4

iropoda Crustacea Amprnpoda Gammandae Gammarus fasciatus S

1 a Insecta Coleoplera Elmidae Stenelmis sp 1

Diptera Chironomidae Chironominae Chironomini Dicrotendipes neomodestus 2

1 Microtendipes caelum 1

Tanytarsini Rheotanytarsus distmctissimus 9

Rheotanytarsus exiguus 15

Tanytarsus sp 1

Orthocladnnae Orthocladiini Tvetema discolonpes 1

1 Ephemeroptera Tncorythidae Tncorythodes sp 2

Tnchoptera Hydropsychidae Cheumatopsyche sp 7

Hydropsyche bettem 10

Hydroptilidae Hydroptilinae Hydroptila sp 1

Leptocendae Ceraclea sp 1

Mysfaodes sp 1

ABS Page 17 of 30

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

25001

ASBRV-UPB-7-5

Vegetation sand detritus

DatenTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 22000 PM

100

Not Reported

Phylum

Anhropoda

Class

Insects

Order

Diptera

Family

Tipulidae

Sub-Family Tribe GenusSpeciesVariety

Antocha sp

Counted

2

Ephemeroptera Baetidae Acentrella sp 4

Baetis sp 6

Tncorythidae Tncorythodes sp 3

Tnchoptera Hydropsychidae Cheumatopsyche sp 52

Hydropsyche betteni 55

Symphitopsyche spama 2

Leplocendae Tnaenodes sp 1

Philopotamidae Chimaira obscure 22

Sub-Total

Grand Total

504

504

ATTACHMENT B

STATISTICA OUTPUT

CL

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Appendix B ANOVA Results

Analysis of Variance (Benthics) Marked effects are significant at p lt 05000

SS df MS SS df MS F P Variable Effect Effect Effect Error Error Error Total Abundance 700779 7 2 50089 87 94966 00 72 7973 833 6 329406 0 073282 No of Taxa 336 2 1680 27280 12 22733 0739003 0498120 Dominant Species Fraction 00 2 000 016 12 0013 0076844 0926486 SW Index 00 2 000 013 12 0011 0359782 0705106 Eveness 00 2 000 010 12 0008 0 567033 0581691

Appendix B Tukey Test

Tukey HSD test Variable Total Abundance (Benthics) Marked differences are significant at p lt 05000

Sample ID 1

M=12360 2

M=96800 3

M=28200 ASBRV-TB-31 0884 0039 ASBRV-TB-6 2 0884 0077 ASBRV-UPB-7 3 0039 0077

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Katherine Fogarty Menzie Cura amp Associates 1 Courthouse Lane Suite 2 Chelmsford MA01824 Reference WR Grace Assabet River

Date 82903 BTR No 07238 Project No 03054 No of Samples 15 Date Received 8603

Laboratory Sample ID

Client Sample ID

Remarks

25001

ASBRV-UPB-7-5

Vegetation sand detritus

DatenTime Sample Collected

Percent Sample Examined

Sampling Depth (m)

8403 22000 PM

100

Not Reported

Phylum

Anhropoda

Class

Insects

Order

Diptera

Family

Tipulidae

Sub-Family Tribe GenusSpeciesVariety

Antocha sp

Counted

2

Ephemeroptera Baetidae Acentrella sp 4

Baetis sp 6

Tncorythidae Tncorythodes sp 3

Tnchoptera Hydropsychidae Cheumatopsyche sp 52

Hydropsyche betteni 55

Symphitopsyche spama 2

Leplocendae Tnaenodes sp 1

Philopotamidae Chimaira obscure 22

Sub-Total

Grand Total

504

504

ATTACHMENT B

STATISTICA OUTPUT

CL

ltrgt o 0

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Appendix B ANOVA Results

Analysis of Variance (Benthics) Marked effects are significant at p lt 05000

SS df MS SS df MS F P Variable Effect Effect Effect Error Error Error Total Abundance 700779 7 2 50089 87 94966 00 72 7973 833 6 329406 0 073282 No of Taxa 336 2 1680 27280 12 22733 0739003 0498120 Dominant Species Fraction 00 2 000 016 12 0013 0076844 0926486 SW Index 00 2 000 013 12 0011 0359782 0705106 Eveness 00 2 000 010 12 0008 0 567033 0581691

Appendix B Tukey Test

Tukey HSD test Variable Total Abundance (Benthics) Marked differences are significant at p lt 05000

Sample ID 1

M=12360 2

M=96800 3

M=28200 ASBRV-TB-31 0884 0039 ASBRV-TB-6 2 0884 0077 ASBRV-UPB-7 3 0039 0077

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

ATTACHMENT B

STATISTICA OUTPUT

CL

ltrgt o 0

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Appendix B ANOVA Results

Analysis of Variance (Benthics) Marked effects are significant at p lt 05000

SS df MS SS df MS F P Variable Effect Effect Effect Error Error Error Total Abundance 700779 7 2 50089 87 94966 00 72 7973 833 6 329406 0 073282 No of Taxa 336 2 1680 27280 12 22733 0739003 0498120 Dominant Species Fraction 00 2 000 016 12 0013 0076844 0926486 SW Index 00 2 000 013 12 0011 0359782 0705106 Eveness 00 2 000 010 12 0008 0 567033 0581691

Appendix B Tukey Test

Tukey HSD test Variable Total Abundance (Benthics) Marked differences are significant at p lt 05000

Sample ID 1

M=12360 2

M=96800 3

M=28200 ASBRV-TB-31 0884 0039 ASBRV-TB-6 2 0884 0077 ASBRV-UPB-7 3 0039 0077

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

CL

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Appendix B ANOVA Results

Analysis of Variance (Benthics) Marked effects are significant at p lt 05000

SS df MS SS df MS F P Variable Effect Effect Effect Error Error Error Total Abundance 700779 7 2 50089 87 94966 00 72 7973 833 6 329406 0 073282 No of Taxa 336 2 1680 27280 12 22733 0739003 0498120 Dominant Species Fraction 00 2 000 016 12 0013 0076844 0926486 SW Index 00 2 000 013 12 0011 0359782 0705106 Eveness 00 2 000 010 12 0008 0 567033 0581691

Appendix B Tukey Test

Tukey HSD test Variable Total Abundance (Benthics) Marked differences are significant at p lt 05000

Sample ID 1

M=12360 2

M=96800 3

M=28200 ASBRV-TB-31 0884 0039 ASBRV-TB-6 2 0884 0077 ASBRV-UPB-7 3 0039 0077

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B ANOVA Results

Analysis of Variance (Benthics) Marked effects are significant at p lt 05000

SS df MS SS df MS F P Variable Effect Effect Effect Error Error Error Total Abundance 700779 7 2 50089 87 94966 00 72 7973 833 6 329406 0 073282 No of Taxa 336 2 1680 27280 12 22733 0739003 0498120 Dominant Species Fraction 00 2 000 016 12 0013 0076844 0926486 SW Index 00 2 000 013 12 0011 0359782 0705106 Eveness 00 2 000 010 12 0008 0 567033 0581691

Appendix B Tukey Test

Tukey HSD test Variable Total Abundance (Benthics) Marked differences are significant at p lt 05000

Sample ID 1

M=12360 2

M=96800 3

M=28200 ASBRV-TB-31 0884 0039 ASBRV-TB-6 2 0884 0077 ASBRV-UPB-7 3 0039 0077

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Tukey Test

Tukey HSD test Variable Total Abundance (Benthics) Marked differences are significant at p lt 05000

Sample ID 1

M=12360 2

M=96800 3

M=28200 ASBRV-TB-31 0884 0039 ASBRV-TB-6 2 0884 0077 ASBRV-UPB-7 3 0039 0077

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Cell No Sample ID 1

12360 1 ASBRV-TB-3 2 ASBRV-TB-6 0851 3 ASBRV-UPB-7 0028

Note that field designation ASBRV-TB-3 is equivalent to ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc

Appendix B Dunnetts Test on Total Abundance

Dunnett test variable Total Abundance (Benthics) Probabilities for Post Hoc Tests (2-sided) Error Between MS = 79138 df = 12000

Sample ID 3 Cell No 28200 1 ASBRV-TB-3 0028 2 ASBRV-TB-6 0072 3 ASBRV-UPB-7

Note that field designation ASBRV-TB-3 is equivalent to sample location ASBRV-T-3 etc