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7/25/2019 Meningococ Si Gonococ Lp UTM
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Genul Neisseria
Diagnosticul de laborator in infectii produse deNeisseria gonorrhoeae
Diagnosticul de laborator in infectii produse deNeisseria meningitidis
1)Neisseria gonorrhoeae
Produs patologic:
secreie uretral, secreie glande Bartholini, secreie vaginal, secreie prostatic
la femei se recolteaz n timpul sau la sfrsitul ciclului menstrual
secreie faringian
urin
secreie conjunctival
2)Neisseria meningitidis
Produs patologic:
LCR
secreie faringian
snge
Rezistena: foarte sensibili la temperatura camerei, la uscciune ,recoltarea se face la
patul bolnavului
1. Examenul microscopic, frotiu din produsul patologic
N. gonorrhoeae
o coci !ram"#$ sub form de boab de cafea dispusi diplo
o n infecii acute se gsesc intracelular
o n infecii cronice se gsesc intra si e%tracelular&
'
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Slide 1
Neisseria gonorrhoeae
(Enlarged view)
Slide 2
Nongonoccal urethritis
(Enlarged view)
Slide 3
Chlamydia trachomatis
(Enlarged view)
These images are from the original published atlas: Tenover, F. C., and J. V. Hirschmann. !!".
#nterpretation of $ram stains and other common microbiologic slide preparations. The %pJohn Company,
&alama'oo, (ich. )ermission granted to the *+( (icrobeibrary by )fi'er #nc.
Neisseria Gonorrhoeae
(
http://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpghttp://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-1.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-2.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpghttp://lib.jiangnan.edu.cn/ASM/029-3.jpg7/25/2019 Meningococ Si Gonococ Lp UTM
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N. meningitidis
o coci !ram"#$ sub form de boab de cafea dispusi diplo
o ncapsulai
Neisseria meningitidis Gramstain of a pure culture. Note t!e c!aracteristic "diplococcal"
arrangement of cells.
textbookofbacteriology.net/.../meningitis.html
meningococ
)
http://textbookofbacteriology.net/themicrobialworld/meningitis.htmlhttp://www.dstl.gov.uk/science_spot/acolyte.phphttp://textbookofbacteriology.net/themicrobialworld/meningitis.html7/25/2019 Meningococ Si Gonococ Lp UTM
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#. $zolarea "sunt germeni foarte preteniosi$
se nsmneaz imediat dup recoltare
se nclzeste mediul la )*+C mediile de cultur utilizate !eloz#chocolat, -haier#.artin
Neisseria gonorrhoeae (ATCC43069) colonies growing on Thayer Martin Modi!ied
Agar (Cat. no. "30). #nc$%ated in C&'!or 4 ho$rs at 3 deg. C.
$ncubarea se face cu % obligatoriu 1'( #))* ore la +'%.
/
7/25/2019 Meningococ Si Gonococ Lp UTM
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+. $dentificarea
%aractere de cultur-
col 012 friabile, asemntoare perlelor&
ncadrarea /n genul Neisseria:
-estul o%idazei este pozitiv 3 4eisseria
5
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Reactia catalazei este pozitiva
Colonia testa pe o lama cu o picatura de )6 apa o%igenata
Reactie pozitiva 3 apar bule
7
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8ermentarea zaharurilor
Gonococ
!L9C:;< =
.>L-:;<
0eningococ
!L9C:;< =
.>L-:;< =
ncadrarea /n specie:
-estul de umflare a capsulei
4 meningitidis pt stabilirea erogrupului
*
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). ntibiograma se face pe medii speciale, conform CL1?&
2. Diagnostic rapid
bazat pe reacii >g#>c se pun n eviden antigenele meningococice direct din LCR
o Reacii de precipitare inelar
o Reacii de aglutinare&
Modul de desfasurare a lucrarii practice
studentii primesc manuscrisul cu Diagnosticul de laborator in infectii produse de
Neisseria gonorrhoeae.Diagnosticul de laborator in infectii produse deNeisseria
meningitidis
Lp se desfasoara interactiv- se discuta pe marginea manuscrisului primit
anterior LP-ului
'& Fiecare student prinde imaginea la microscop si examineaza frotiuri :coci
Gram negativi reniformi in diplo
Bibliografie
Bacteriologie Medicala, Olga Dorobat, editura UTM, 2006
Clinical Microbiology Procedures Handbook, Henry D. Isenberg, editata American Society for
Microbiology, 2004
Koneman's Color Atlas and Textbook of Diagnostic Microbiology,Washington C
Winn, Stephen D. Allen, Gail L. Woods,William M. Janda, Paul C. Schreckenberger, editura Lippincott
Williams & Wilkins, 2005
Manual of Clinical Microbiology , Patrick R. Murray,
Visit Amazon's Patrick R. Murray Page
search results
Learn about Author Central
@
http://shopping.yahoo.com/search;_ylt=AmwIkvVSDn3oMrp235b4BMACyNsA?p=Henry+D.+Isenberghttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Stephen+D.+Allenhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Gail+L.+Woodshttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_1http://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_pop_1http://www.amazon.com/s/ref=ntt_athr_dp_sr_pop_1?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Patrick%20R.%20Murrayhttp://authorcentral.amazon.com/gp/landing/ref=ntt_atc_dp_pel_1http://shopping.yahoo.com/search;_ylt=AmwIkvVSDn3oMrp235b4BMACyNsA?p=Henry+D.+Isenberghttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://shopping.yahoo.com/search;_ylt=AkzkLWrc9z31QkUKO_a6ltICyNsA?p=American+Society+for+Microbiologyhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Washington+C+Winnhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Stephen+D.+Allenhttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Gail+L.+Woodshttp://search.barnesandnoble.com/booksearch/results.asp?ATH=William+M.+Jandahttp://search.barnesandnoble.com/booksearch/results.asp?ATH=Paul+C.+Schreckenbergerhttp://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_1http://www.amazon.com/Patrick-R.-Murray/e/B001IGOFSS/ref=ntt_athr_dp_pel_pop_1http://www.amazon.com/s/ref=ntt_athr_dp_sr_pop_1?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Patrick%20R.%20Murrayhttp://authorcentral.amazon.com/gp/landing/ref=ntt_atc_dp_pel_17/25/2019 Meningococ Si Gonococ Lp UTM
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Ellen Jo Baron,Michael A. Pfaller, Fred C. Tenover,Robert H. Yolken, editura ASM, 2007
Performance Standards for Antimicrobial Susceptibility Testing.M 100, S19
CLSI /2009
A
http://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolkenhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolkenhttp://www.amazon.com/Ellen-Jo-Baron/e/B001HCVG58/ref=ntt_athr_dp_pel_2http://www.amazon.com/s/ref=ntt_athr_dp_sr_3?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Michael%20A.%20Pfallerhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_4?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Fred%20C.%20Tenoverhttp://www.amazon.com/s/ref=ntt_athr_dp_sr_5?_encoding=UTF8&sort=relevancerank&search-alias=books&field-author=Robert%20H.%20Yolken