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MEDILEG Project 2012-2015 “Breeding, agronomic and biotechnological approaches for reintegration and revalorization of legumes in Mediterranean agricultureCoordination of the Agricultural Research in the Mediterranean Area

MEDILEG, Breeding, agronomic and biotechnological approaches

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Page 1: MEDILEG, Breeding, agronomic and biotechnological approaches

MEDILEG Project

2012-2015

“Breeding, agronomic and biotechnological approaches for reintegration and

revalorization of legumes in Mediterranean agriculture“

Coordination of the Agricultural Research in the Mediterranean Area

Page 2: MEDILEG, Breeding, agronomic and biotechnological approaches

MEDILEG Project 2012-2015

Legumes are multifunctional crops with extraordinary importance for the agriculture, environment and culture

in Mediterranean countries

However acreage is continuosly decreasing

and we are importing legumes for food and feed

Page 3: MEDILEG, Breeding, agronomic and biotechnological approaches

MEDILEG Project 2012-2015

Cooperative multidisciplinary initiative to promote legume revalorization in Mediterranean agriculture

Page 4: MEDILEG, Breeding, agronomic and biotechnological approaches

The main objective of this project is to develop methodologies to improve grain legume plant material of high yield potential, resistant to major diseases and abiotic stresses, suitable for sustainable farming systems.

The selection of genotypes carrying the desired genes will be greatly facilitated by using molecular markers tightly linked to the desired traits. In this sense, pea will be used as a model as the best characterized crop legume.

(I) Definition of ideal genotypes, (II) Studies on biotic constraints(III) Studies on abiotic stresses(IV) Marker Assisted Selection (MAS)(V) Epidemiology(VI) Exploitation of alellopathy for disease control.

MEDILEG Project

“Breeding, agronomic and biotechnological approaches for reintegration and revalorization of legumes in Mediterranean agriculture“

Page 5: MEDILEG, Breeding, agronomic and biotechnological approaches

WP0: Research coordination of the consortium Financial management will be handled directly between national researchers and

their national funding organizations in each participating country.

T0.1: Facilitate close interaction between the Partners being the single point of contact between the ARIMNet Call Office and the Researchers

T0.2: Draw a Consortium Agreement in order to manage the delivery of the project activities, finances, Intellectual Property Rights (IPR) and to avoid disputes which might be detrimental to the completion of the project

T0.3: Redirect specific parts of the project if required by the progress of the Work packages

T0.4: Compile and submit reports/deliverables to the ARIMNet Office on behalf of the Research Consortium (M6, M18, M36)

T0.5: Meeting calendar: kick-off, mid-term and final meetings

Page 6: MEDILEG, Breeding, agronomic and biotechnological approaches

WP1: Identification of regional priorities

and definition of target genotypes

Coordinator: Dr Mohamed Kharrat, INRAT Tunis

D1.1: Definition of phenotypes and genotypes adapted to sustainable agriculture demands in different regions

D1.2: Information and protocols for field evaluation of agronomic traits and resistance to biotic and abiotic constrains

D1.3: Selection of the most representative lines/varieties that might serve as source gene-pool for genotype enhancement

Page 7: MEDILEG, Breeding, agronomic and biotechnological approaches

WP2: Studies relevant for breeding for resistance to major biotic constraints

Coordinator: Prof. Diego RUBIALES, CSIC, Spain

D2.1: Rapid and reliable screening methods suitable for selection and breeding

D2.2: Development of breeding material with improved resistance tested at a wide range of environments and pathogen populations

D2.3: Catalogue of sources of resistance

Page 8: MEDILEG, Breeding, agronomic and biotechnological approaches

WP3: Studies relevant for breeding for major abiotic constraints

Coordinator: Dr Marion PRUDENT, INRA-LEG, France

D3.1: Standardized experimental conditions for drought and salt tolerance

D3.2: Rapid methods for screening for drought and salinity tolerance

D3.3: Identification of sources of resistance to drought and salinity (on various species, and in combination with particular Rhizobium strains)

Page 9: MEDILEG, Breeding, agronomic and biotechnological approaches

WP4: Marker Assisted Breeding

Coordinator: Dr. Alain BARANGER, INRA-IGEPP, France

D4.1: List of polymorphic eSSR and SNP markers for genetic mapping and pea map comparisons

D4.2: A set of SNP markers in pea designed in genes involved in stress resistance and plant architecture and development

D4.3: High density pea linkage maps from populations segregating for resistance biotic and abiotic factors

D4.4: Identification, and determination of the genetic effects of the QTLs controlling polygenic traits

D4.5: Information on QTL stability across diverse field locations, pathogen variability and plant genetic backgronds

D4.6: Development of standard markers for pyramiding and rapid screening

D4.7: List of candidate genes likely to be involved in resistances to stresses.

Page 10: MEDILEG, Breeding, agronomic and biotechnological approaches

WP5: EpidemiologyCoordinator: Dr. Christophe LE MAY, INRA-IGEPP, France

D5.1: Clarification of the genetic variation and adaptive potential of ascochyta species

D5.2: Identification of new control levers to better control disease development.

D5.3: Determine control method combinations that will decrease disease development.

D5.4: Identification of architectural features that will limit the spatiotemporal dispersal of the disease.

D5.5: Development of standard differential sets for race identification.

Page 11: MEDILEG, Breeding, agronomic and biotechnological approaches

WP6: Biological control and allelopathy studiesCoordinator: Prof. Antonio EVIDENTE, UNINA, Italy

D6.1: Protocols for the extraction and purification of bioactive metabolites (phytotoxins, herbicides, phytoalexins)

D6.2: Structure determination of new bioactive compounds with original carbon skeleton

D6.3: Characterization of phytotoxic, antifungal, mycotoxic and allelopatic activities of the pure metabolites isolated

D6.4: Preparation of key derivatives for structure confirmation and structure-activity relationships studies

Page 12: MEDILEG, Breeding, agronomic and biotechnological approaches

WP7: DisseminationCoordinato: Dr. Lamiae GHAOUTI, IAV, Morocco

D7.1: Methodology and biotechnological protocols transference to facilitate the selection of interesting genotypes

D7.2: Scientific papers, divulgation articles in farmer journals and contributions to conferences

D7.3: Project web site

Page 13: MEDILEG, Breeding, agronomic and biotechnological approaches

T0.2: Draw a Consortium Agreement in order to manage the delivery of the project activities, finances, Intellectual Property Rights (IPR) and

to avoid disputes which might be detrimental to the completion of the project

- 1st draft circulated 15th June 2012

- Comments requested for 29th June 2012. Almost completed

Contact person:

Francisco OROZCOConsejo Superior de Investigaciones CientíficasLegal Unit for European Programmes Serrano, 113 – 28006, [email protected]: 0034 91 568 1915

Page 14: MEDILEG, Breeding, agronomic and biotechnological approaches

T0.5: Meeting calendar:

- Kick-off meeting: Tunis 4-6 September 2012

Page 15: MEDILEG, Breeding, agronomic and biotechnological approaches

T0.5: Meeting calendar:

- Kick-off meeting: Tunis 4-6 September 2012

- Mid-term meeting:Suggestions: - Novi Sad, Serbia, as satellite of 1st Legume Society meeting May 2013

too early?? Better close to M18 report?? Venue??

- Final meeting: - close to M36 report; venue??

Page 16: MEDILEG, Breeding, agronomic and biotechnological approaches

T7.3: MEDILEG project website for dissemination

Page 17: MEDILEG, Breeding, agronomic and biotechnological approaches

Institute for Sustainable Agriculture Córdoba, Spain

Page 18: MEDILEG, Breeding, agronomic and biotechnological approaches

STRESS RESISTANCE GROUPDiego RUBIALES

Dr. Castillejo

Proteomics

Dr. Fondevilla

Genomics

Ascochyta,

powdery mildew

Dr. Barilli

Mapping

Intercropping

rust

Dr. Cobos

Mapping

Ascochyta,

Fusarium

Dr. Rispail

Genomics

Biocntrol

Fusarium

Dr. Fernandez-Apar.

Genomics

Intercropping

Orobanche

Dr. Prats

Drought

Physiology

Dr. Moral

Epidemiology

>210 SCI publications mostly on resistance to diseases out of which:

> 130 in legumes

> 70 in cereals

> 5 others (roses, sunflower, sugar beet, garlic)

Dr. Villegas

Genomics

Botrytis