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Medical Parasitology lab
Entamoebia
• Constantly change in shape
1. Intestinal
• Pathogenic E. histolytica
• No pathogenic (E. coli (>4 nuclei in the cyst)
2. Free living
• Feco-oral rout
E hostylitica
• Habitat
• Mucosa and submucosa of large intestine
• 3 stage
• Trophozoite
• Precyst : intermediate stage between Trophozoite and cyst
• Cyst
Trophozoite
• invasive form• Passed only of patient with active dysentery• Its size is 15-30 µm.• The cytoplasm has 2 zones
• a hyaline outer margin (ectoplasm) • a granular inner region that may contain red
blood cells ,food vacuoles (endoplasm). • Single nucleus, spherical contain central dot
(karysome)• It has pseudopodia: long finger like projection of
endoplasm through ectoplasm• Motile: active unidirectional progressive and
purposeful movement
Cyst
• Infective form found in formed stool• Cysts are present in the lumen of the
colon and in formed feces.• Subspherical cysts of pathogenic
amebas range from 10-20 µm. • Smaller cysts 10 -3.5µm are
considered nonpathogenic (E. hartmanni).
• Cysts may contain 1-4 nuclei, a glycogen vacuole and chromatoidalbodies with characteristic rounded ends.
• Covered with smooth chitinous wall
Flagellate
• Tissue flagellat:
• Giardia lamblia (Intestinal)
• Trichomonas vaginalis (STD)
• The hemoflagellates of humans
• Trypanosoma
• Leishmania
Giardiasis
• Highest in developing countries
• Highest in children
Trophozoite• heart-shaped, symmetric, 10-20 µm
in length, • 4 pairs of flagella,• 2 nuclei with prominent central
karyosomes, • A large concave sucking disk in the
anterior portion occupies much of the ventral surface.
• The swaying or dancing motion of trophozoites in fresh preparations is unmistakable.
Cysts • found in the stool-often in enormous
numbers. • As the parasites pass into the colon, they
typically encyst.• They are thick-walled, highly resistant,• 8-14 µm in length, • contain 2 nuclei as immature & 4 as mature
cysts.
Giardia lamblia
Trophozoites Cysts
Trichomoniasis
• Trophozoites of Trichomonas vaginalis
are
• Oval shape
• 7-30 µm long.
• They have five flagella: four anteriorly
directed flagella and one posteriorly
along the outer membrane of the
undulating membrane.
• The large nucleus is usually located at
the wider, anterior end and contains
many chromatin granules and a small
karyosome.
• It moves with wobbling and rotating
motion
T. Vaginalis trophozoite
• Oval shape typically measure 7-15um
• Fg=flagella
• Bb=basal body
• Nu=nucleus (single elongated nucleus)
• Ax=axostyle
• um=undulating membrane
• Cy=cytostomal groove
• Cs=costa
Helminthes
• Helminths are invertebrates characterized by elongated, flat or round bodies.
• Platyhelminthes (flat) (hermaphrodite)
• Trematodes (flukes)
• Cestodes (tapeworms )
• Nemathelminthes (circular)
• Nematodes (Roundworms)
Trematodes
• Flat
• Suckers
• Digestive system
• Genetal system (testes and uterus)
• Intermediate host : snail
Schistosomiasis (‘bilharziasis)
• Schistosomiasis (‘bilharziasis’) is an infection with parasitic blood flukes:
Schistosoma mansoni, S. haematobium, S. japonicum,
Cestodes
• Flat, segmented, tape worm
• Scolics
• Neck dividing
• Up to 10 meter
• No digestive system
• Gental system (male, female)
• Immature segment, mature segment, gravid segment
Echinococcus granulosus and Echinococcus
multilocularis
• Echinococcus granulosus and Echinococcus multiloculariscause echinococcosis (Hydatid disease), common in central and Eastern Europe
• Patients presenting with primary infection are often asymptomatic. Latent infections may present years later with symptoms caused by mass effect within organs, obstruction of blood or lymphatic flow, or complications such as rupture or secondary bacterial infections. Fever and acute hypersensitivity reactions, including anaphylaxis, may be the principal manifestations of cyst rupture.
Nematodes
• Female or male
• Cylindrical
• 4 tubes
• Digestive system, genital system, excretory system, nervous system.
Ascaris lumbricoides
Infect by eggs that are strongly resistant to desiccation &
environmental conditions.
• Eggs are 75x40 µm, with a thick mamillated brownish shell.
• Freshly passed eggs in feces are not infective, they require 2-3
weeks to develop to be embryonated (contain larva).
• Male
• 15-30cm * 2-4mm
• Posterior end curved to form hook
• Femal
• 20-40cm *3-6mm
• Posterior end straight
• Distinct groove is present at valvar opening
• 2,00,000 eggs per day
• 2 type of eggs
• Fertilized egg
• Embyronated unsegmented ovum
• Develop into the infective form
• Round oval
• Unfertilized
• Atrophied ovum
• Elliptial in shape
• larger
Unfertilized egg
Elongated
Thin shelled
Disorganized internal content
Ascaris lumbricoides
Unfertilized egg
Elongated
Thin shelled
Disorganized internal
content
Egg containing a larva-
infective if ingested
Adult worms
Trichuris trichiura• Whip-worm, adults 3-7cm, live in caecum & colon of man.
• Eggs are barrel-shaped, 50x25 µm with a thick brown shell and a
mucoid plug at each pole. They are passed in feces to complete
development in soil as in Ascaris. This emryonated egg is the infective
stage.
Adults of T. trichiura Egg of T. trichiura
Enterobius vermicularis (Pinworm)
Small nematode, a common parasite among children, it is unique in that
eggs are viable shortly after being laid directly on the perianal skin. They
can be accidentally ingested, or passed on fingers, clothing or fecal flecks
to others.
Itching around the anal or vaginal areas are the most common pinworm
symptoms. Suspect a pinworm infection if the child shows night time
itching in these areas.
Pinworm EggsAn adult pinworm An adult pinworm