mayk keyler

7/17/2019 mayk keyler

http://slidepdf.com/reader/full/mayk-keyler 1/8

NST TUC ÓN EDUCAT VA” M NSA”

TRABAJO: EXTRACC ON CASERA DEL ADN

N Y AP: MAYK KEYLER CR SOSTOMO ROJAS

GRADO: 6°

INTRODUCCION:

Este p!"e#t! se $% e%&'(%)! #!* &% +'*%&')%) )e p!)e

S%,e #!-pe*)e " e.pe'-e*t% s!,e &% e.t%##'/*

s!,e e& ADN0

Y #!* e& %p!"! )e -' p%)e &! pese*t%-!s este

p!"e#t! p%% e& p1,&'#!0



EXTRACCIÓN CASERA DE ADNE23'p! I*4est'5%)!:

Est3)'%*te:

MAYK KEYLER CRISOSTOMO ROJAS

ASESOR:

UGO CRISOSTOMO PERE7

80 PLANTEAM ENTO DEL PROBLEMA

8080 P!,&e-%:

9De 23 -%*e% p!)e-!s e.t%e " !,se4% e& ADN )e 3* p!)3#t!

*%t3%&;

80<0 O,=et'4!s:

Ut'&'(% 3*%s se*#'&&%s t#*'#%s p%% p!)e e.t%e e& ADN )e 3*p!)3#t! *%t3%&

O,se4% &% est3#t3% +','&% )e& ADN0

80>0 J3st'+'#%#'/*:

L% '*4est'5%#'/* e%&'(%)% se4'? )e -3#$!@ e* -3#$%s '*st't3#'!*es

"% 23e #%e#e* )e -%te'%& )e &%,!%t!'! #!-! e* este #%s! e&

-'#!s#!p'! 23e es 3* p!#! #!st!s! " t%-,'* p%% 23e s!#'%&'(% e&

#!*#ept! 23e e* pespe#t'4% " 3*% 4e( %pe*)')! p! &% p!,&%#'/*

p3e)%* e*te*)e &%s )'st'*t%s e*+e-e)%)es #!*5*'t%s@ )e es%

http://feria-ciencias-elvis.blogspot.com/2015/07/extraccion-casera-de-adn.html

http://feria-ciencias-elvis.blogspot.com/2015/07/extraccion-casera-de-adn.html



-%*e% se p3e)% e&%,!% &% #%&')%) )e 4')% t!-%*)! -e)')%s

pe4e*t'4%s0

<0 MPORTANC A

L% +3*#'/* p'*#'p%& )e& ADN es -%*te*e % t%4s )e& #/)'5! 5e*t'#!&% '*+!-%#'/* *e#es%'% p%% #e% 3* se 4'4! ')*t'#! % %23e& )e&

23e p!4'e*e B! -3" s'-'&% e* e& #%s! )e -e(#&%se #!* !t% #%)e*%

#!-! es e& #%s! )e &% ep!)3##'/* se.3%& ! )e s3+' -3t%#'!*es0

P! s3s +3*#'!*es " p!p'e)%)es e*te e&&%s p!)e-!s p!)e-!s

es%&t% 23e:

80 E& ADN #!*t!&% &% %#t'4')%) )e &% #&3&%0

<0 E* #'et!s #%s!s #!-1*-e*te )e'4%)!s )e& #%s! %*te'! e& ADN

p3e)e &&e5% % te*e #'et% #!*)3#t'4')%) se51* 3* est3)'! e%&'(%)!0

P! t!)! &! -e*#'!*%)! #%,e )est%#% &% '-p!t%*#'% )e estep!"e#t! e* &% e.t%##'/* )e ADN p%% t%t% )e e*te*)e 23e t%*

s'5*'+'#%t'4! es e& #!*!#e s!,e este te-% )e& ADN0

>0 MARCO TEÓR CO

93 es e& ADN;

ADN es &% %,e4'%t3% )e& ?#')! )es!.'',!*3#&e'#! Be* '*5&s DNA0

C!*st't3"e e& -%te'%& 5e*t'#! )e &!s !5%*'s-!s0 Es e& #!-p!*e*te

23F-'#! p'-%'! )e &!s #!-!s!-%s " e& -%te'%& )e& 23e &!s 5e*esest?* +!-%)!s0

E& ADN +3e ')e*t'+'#%)! '*'#'%&-e*te e* 86 p! H'e)'#$ M'es#$e

,'/&!5! s3'(! e* &!s *1#&e!s )e &%s #&3&%s )e& p3s !,te*')%s )e &!s

4e*)%=es 23'15'#!s )ese#$%)!s " e* e& espe-% )e& s%&-/*0 & &&%-/

% &% s3st%*#'% *3#&eF*% %3*23e *! +3e e#!*!#')% $%st% 8> 5%#'%s

%& e.pe'-e*t! e%&'(%)! p! Os%&) A4e"

L% est3#t3% )e& ADN es 3*% p%e=% )e &%5%s #%)e*%s )e

*3#&e/t')!s0 L% est3#t3% )e )!,&e $&'#e )e& ADN +3e )es#3,'et% e*

8> p! J%-es %ts!* " H%*#'s C'#0 U*% &%5% $e,% )e ?#')!*3#&e'#! est? e*!&&%)% %&e)e)! )e !t% $e,% +!-%*)! 3* p%

e*te&%(%)!0 D'#$% $&'#e -')e > *- )e p%s! )e !s#% " <> *- )e

)'?-et! " est? +!-%)% e* #%)% 43e&t% p! 8Q p%es )e

*3#&e/t')!s e*+e*t%)!s e*te sF p! s3s ,%ses *'t!5e*%)%s0

S/&! te*e-!s 3* >Q -?s )e 5e*es 23e 3* )'-'*3t! 53s%*! e& #3%&

t'e*e s!&! 3* -'&F-et! )e &!*5't3) " p!see #&3&%s e* t%*t! 23e

*!s!t!s %&e)e)! )e 8QQ ,'&&!*es0 Y -'e*t%s 23e &!s sees $3-%*!s

p!see-!s %p!.'-%)%-e*te 8QQ0QQQ -'&&!*es )e *e3!*%s e&

)'-'*3t! 53s%*! #3e*t% 1*'#%-e*te #!* >Q<0



E* #!-p%%#'/* #!* e& %!( *!s 4% %1* pe! p3est! 23e 3* 5%*! )e

%!( t'e*e -3#$!s -?s 5e*es 23e 3*% pes!*% " t!)%4F% *! se &e $%

!#3')! *' &% -?s t!*t% )e &%s ')e%s0

9UI ES UNA EN7 MA;

L%s e*('-%s s!* p!teF*%s 23e %"3)%* % 23e &%s e%##'!*es 23F-'#%s

!#3%* #!* -%"! %p')e(0 S'* e*('-%s *3est!s #3ep!s se

)ete*)F%* e* se#!0

E* este e.pe'-e*t! &%s e*('-%s 23e est%-!s 3t'&'(%*)! p!4'e*e*

)e& %,&%*)%)! )e #%*es " #!t%* &%s p!teF*%s t%& #!-! 3* p% )e

t'=e%s0

Desp3s )e& p%s! )e& )ete5e*te &% 1&t'-% pe53*t% +3e: 93 es &!

23e t'e*es e* t3 s!p% )e %4e=%s;

L%s -e-,%*%s #e&3&% " *3#&e% $%* s')! !t%s %& '53%& 23e t!)%s &%s-e-,%*%s )e &!s !5%*e&!s #!-! &%s 23e !)e%* % &%s -'t!#!*)'%s

" #&!!p&%st!s0 E*t!*#es 923 es &! 23e 23e)%;

P!teF*%s

C%,!$')%t!s B%(1#%es

ADN

E& ADN e* e& *1#&e! )e &% #&3&% est? -!&)e%)! )!,&%)! " p!te5')!

p! p!teF*%s0 E& %,&%*)%)! )e #%*e #!t% &%s p!teF*%s

sep%?*)!&%s )e& ADN0

HORMULAC ÓN DE LA PÓTES S

80 L% e.t%##'/* )e ADN e23'ee 3*% se'e )e et%p%s ,?s'#%s: E*

p'-e &35% t'e*e 23e !-pese &% p%e) #e&3&% " &% -e-,%*%

P&%s-?t'#% p%% p!)e %##e)e %& *1#&e! )e &% #&3&%0 A #!*t'*3%#'/*

)e,e !-pese t%-,'* &% -e-,%*% *3#&e% p%%

De=% &',e e& ADN0 L!s )ete5e*tes 3t'&'(%)!s #!-! &%4%4%='&&%s

e-3&s'!*%* &!s &Fp')!s )e &%s -e-,%*%s #e&3&%es " &%s !-pe*0

<0 L% s%& e4't% &% 3*'/* )e &%s p!teF*%s %& ADN0

>0 P%% %'s&% e& ADN $%" 23e $%#e 23e pe#'p'te e* %&#!$!&0 E& ADN

es s!&3,&e e* %53% pe! #3%*)! se e*#3e*t% e* %&#!$!& se)ese*!&&% " pe#'p't% e* &% '*te+%#e e*te e& %&#!$!& " e& %53%0



A)e-?s )e pe-'t'*!s 4e e& ADN e& %&#!$!& sep%% e& ADN )e

Ot!s #!-p!*e*tes #e&3&%es &!s #3%&es s!* )e=%)!s e* &% s!&3#'/*

%#3!s%0

0

MATER ALES Y MITODO

MATER ALES:

A&#!$!& 6° -3" +'!

Dete5e*te &F23')!

E*('-%s B%,&%*)%#%*e BS' *! t'e*es 3s% =35! )e p'% ! s!&3#'/*

&'-p'% &e*t'&&%s

S%& )e -es%

A53% )est'&%)% ! -'*e%&

H3e*te )e ADN B4e5et%&es #%*e et#0

V%s!s L'#3%)!%

C!&%)!

T3,! )e e*s%"! )e 4')'!

MITODO: Te/'#! e.pe'-e*t%&

0 PROCED M ENTO

P%% este e.pe'-e*t! pe+e'-!s 3s% %4e=%s 4e)es0 A #!*t'*3%#'/*

&!s p%s!s % se53' p%% &% !,te*#'/* )e& ADN:

P!* e* 3*% &'#3%)!%:

T3 +3e*te )e ADN B-?s ! -e*!s 8QQ -& ! 8W<

)e t%(% )e %4e=%s

U* pe&&'(#! 5%*)e )e s%& )e -es% B-e*!s )e 8 -& ! 8W )e

#3#$%%)'t%

A53% +F%0 E& )!,&e )e &% #%*t')%) )e t3 +3e*te )e ADN B-?s ! -e*!s

<QQ -& ! 8 )e t%(%



L'#3% t!)! % %&t% 4e&!#')%) p! 8 se53*)!s0

E& &'#3%)! sep%% &%s #&3&%s )e &%s %4e=%s 3*%s )e !t%s p! &! 23e

%$!% t'e*es 3*% -3" )'&3')% s!p% )e #&3&%s )e %4e=%s0

V'ete t3 s!p% )e #&3&%s )e %4e=%s % t%4s )e 3* #!&%)! )e*t! )e

!t! #!*te*e)! B#!-! 3*% t%(% -e)')!% p! e=e-p&!0

9C3?*t% s!p% )e %4e=%s t'e*es; A%)e #!-! 8W6 )e es% #%*t')%) )e

)ete5e*te &F23')! B-?s ! -e*!s >Q -& ! )!s #3#$%%)%s s!pe%s "

-(#&%&!0 De=% ep!s% &% -e(#&% e*te " 8Q -'*3t!s0

V'ete &% -e(#&% e* t3,!s )e e*s%"! 3 e* !t!s #!*te*e)!es

pe23e!s )e 4')'! #%)% 3*! #!-! 8W> &&e*!0

V'ete &% -e(#&% e* t3,!s )e e*s%"! 3 e* !t!s #!*te*e)!es

pe23e!s )e 4')'! #%)% 3*! #!-! 8W> &&e*!0

P3e,% 3s% 3*! )e est!s )ete5e*tes ! e& 23e se% 23e te*5%s %

-%*!0

A%)e 3* pe&&'(23't! )e e*('-% % #%)% t3,! )e e*s%"! " %5Ft%&!

s3%4e-e*te0 'Se #3')%)!s! S' &! %5't%s )e-%s'%)! +3ete !-pe?s

e* ADN $%#'*)!&! -?s )'+F#'& )e 4e0

Us% %,&%*)%)! )e #%*e #!-! e*('-%0 S' *! p3e)es e*#!*t%

%,&%*)%)! '*te*t% 3s% =35! )e p'% ! s!&3#'/* &'-p'%)!% p%%

&e*tes )e #!*t%#t!0



L%)e% t3 t3,! )e e*s%"! " &e*t%-e*te 4'ete e& %&#!$!& B's!p!pF&'#!

%& Q ! %&#!$!& etF&'#! s!,e &% p%e) )e& t3,! )e -%*e% 23e

+!-e 3*% #%p% s!,e &% -e(#&% )e %4e=%s0 S'53e 4et'e*)! $%st% 23e

te*5%s e* e& t3,! %p!.'-%)%-e*te &% -'s-% #%*t')%) )e %&#!$!& 23e

)e -e(#&% )e %4e=%s0

E& ADN se e&e4%? )es)e &% -e(#&% )e %4e=%s $%st% &% #%p% )e

%&#!$!&0 P3e)es 3s% 3* p%&'t! )e -%)e% 3 !t! t'p! )e 5%*#$! p%%

%%st% e& ADN 23e est? e* e& %&#!$!&0



E& ADN es 3*% &%5% " pe5%=!s% -!&#3&% % &% 23e &e 53st% +!-%

53-!s0

He&'#'t%#'!*es A#%,%-!s )e #!-p&et% 3*% e.t%##'/* )e ADN

60 RESULTADOS

Se !,t34! 3*% s!&3#'/* %#3!s% 4e)!s% &% #3%& +3e &% -%te'% p'-%

p%% &% !,se4%#'/* )e& ADN0

Se !,se4/ e& ADN )e &%s %4e=%s &% #3%& t'e*e +!-%s $e&'#!')%&es

%&%5%)%s0

E& ADN se %pe#'% )e &% -'s-% +!-% p%% #3%&23'e t'p! )e

4e5et%&es0

0

D SCUS ÓN

Me)'%*te e& p!#e)'-'e*t! e.pe'-e*t%& se !,t34! e& ADN )e &!s

4e5et%&es p3)'e*)! !,se4% s3 +!-%@ &! #3%&es #!*+'-%* !

e%+'-%* &% te!F%0

Desp3s )e 4%'!s e.pe'-e*t!s e* &% !,te*#'/* )e ADN se !,t34! &%

-'s-% +!-% +','&% )e& ADN0

L!s -%te'%&es % 3s% )e,e* se -e)')%s e.%#t%s s'53'e*)! e&

p!#e)'-'e*t! '*)'#%)! p%% *! te*e te*)F%-!s e!es

e.pe'-e*t%&es0

0 CONCLUS ONES

Se 3t'&'(/ t#*'#%s se*#'&&%s 3s%*)! -%te'%&es #%se!s p%% &%

!,te*#'/* )e& ADN )e &!s 4e5et%&es Be* este #%s! %4e=%s0

Se !,t34! &% +!-% +','&% )e& ADN0

0 INDICE

8 #%%t3&%

8Q0 ENUMERACION N° )e $!=%s

Documents

mayk keyler