MAS.S62 FAB2

4.3.12

Self Replicating Biochemical Systems

Holliger et. al

Threshold for LifeWhat is the Threshold for Self Replicating Systems?

Measurement Theory

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Step 1 Step 2 Step 3

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Parts

Template

Machine

Replication Cycle

http://en.wikipedia.org/wiki/File:Stem-loop.svg

Error Correcting Exonuclease

(Ruler)

DNA

Number of Nucleotides

Prob

abili

ty o

f Sel

f Rep

licati

on

NN

N

N

kT

qp

qQp

qQ

kq

q

N

Bond/E-

-1 P :Yield Total

11 :Yield StepPer

:open bonds N ally that Probabilit

3E e :Where

:open is bond single ay that ProbabilitBond

Watson Crick .18 nm

How Well Can N Molecules Measure Distance?

/sandwalk.blogspot.com/2007/12/dna-denaturation-and-renaturation-and.html

200 400 600 800 1000 1200 1400

0.2

0.4

0.6

0.8

1.0

Threshold length: 691 bp for 50% yield. 315 bp for 10-6 yield.

220 ntCombinatorics1:1E132Library Diversity1:1E15

Pligase = 1:2E13

Minimum ribozyme length 165 nt14 nt extensionError rate: 1%Extension rate : 1nt per 153 minutes

Minimum ribozyme length:20 nt extensionError rate: Extension rate :

95 nt base extension