Upload
rosemary-peters
View
216
Download
0
Embed Size (px)
Citation preview
Manipulating and Cloning DNA
Being Healthy
• Type 1 diabetes• Type 2 diabetes• How can you help these individuals?
Insulin
• Banting and Best– Pigs and cows
• Helen Free • Allergic reaction to insulin from pigs and cows• Genetic engineering– Inserted into bacteria – Produced insulin from humans
Why Bacteria?• Reproduce quickly• Plasmids • Form recombinant DNA– Restriction enzymes– Sticky ends vs. blunt ends– Restriction fragments
Sticky or Blunt ends?
How Do You Know Where to Cut?
• Restriction enzymes recognize recognition sites– Sticky ends & blunt ends
• Restriction map
We Are Family
• Vector + foreign material = recombinant DNA– Plasmids
• Competent/host cell takes up recombinant DNA– Bacteria
• Restriction enzymes cut• DNA ligase reforms backbone
Recombinant DNA
Hybridization
Homework
• Page 375 # 2, 3, 5, 8