Magnus Pro Training Magnus Pro Training PresentationPresentation

Image analysis SoftwareImage analysis Software

Note: The contents of this presentation are only showing the procedure for Note: The contents of this presentation are only showing the procedure for calibration, counting & generating reports. The dummy data have been calibration, counting & generating reports. The dummy data have been shown just for training purposeshown just for training purpose

CalibrationCalibration

• For any measurements calibration is required to be For any measurements calibration is required to be performed through standard stage micrometer. performed through standard stage micrometer.

• Formulae : Micrometer scale 1mm = 1000 MicronFormulae : Micrometer scale 1mm = 1000 Micron (e.g. 0.01mm= 10 micron)(e.g. 0.01mm= 10 micron)• It should be performed on all objectives of It should be performed on all objectives of

microscope where digital camera or MIPS are microscope where digital camera or MIPS are installed.installed.

• In case of readjustments or new replacement of In case of readjustments or new replacement of any part of microscope or camera, the calibration any part of microscope or camera, the calibration should be done again.should be done again.

(contd.)(contd.)

Select Magnification before Select Magnification before calibrationcalibration

Before going to Before going to measurement, one has to measurement, one has to

select appropriate select appropriate magnification of calibrationmagnification of calibration

• Focus stage micrometer under the first objective Focus stage micrometer under the first objective of microscope in X direction.of microscope in X direction.

• Capture image with proper names.Capture image with proper names.• Focus stage micrometer under the first objective Focus stage micrometer under the first objective

of microscope in Y direction.of microscope in Y direction.• Capture image with proper names.Capture image with proper names.• Repeat above procedure for any other objectives Repeat above procedure for any other objectives

like 10X, 40X, 100X etc. (as per availability)like 10X, 40X, 100X etc. (as per availability)

(contd.)(contd.)

Calibration Calibration (contd.)(contd.)procedureprocedure

Screen Dumps Screen Dumps (calibration)(calibration)

For objective 100X in X and Y For objective 100X in X and Y direction direction

Load image

captured in X

axis for 100X obj.

Move line slowly with the help of

Zoom Preview and release the line once it

reaches to the other line

Zoom Preview

If line is for e.g.. 60 pixels

Then write down same

reading in the Scale X value

Give the suitable name of calibration

and save

Calibration Procedure for X direction

Load image

captured in Y

axis for 100X obj.

Move line slowly with the help of

Zoom Preview and release the line once it

reaches to the other line

Zoom Preview

If line is for e.g.. 60 pixels

Then write down same

reading in the Scale Y value

Give the suitable name of calibration

and save

Calibration Procedure for Y direction

MeasurementMeasurement

• The measurements can be obtained The measurements can be obtained after pixel calibration.after pixel calibration.

• The units of measurements depends The units of measurements depends on the currently selected spatial on the currently selected spatial calibration (for e.g. micron or inches calibration (for e.g. micron or inches or millimeter etc.)or millimeter etc.)

Measurements of selected images

Images on which

measurements are calculated.

Selected calibration

CountCount

• Used to automatically identify Used to automatically identify objects in an image.objects in an image.

• To get the several feature of the To get the several feature of the measurements.measurements.

Automatically identified objects

in an image

obtained the several

features of the measurements

Click Count to count the no. of

objects

Automatic Report Automatic Report GenerationGeneration

Click Report data and select

appropriate data on which report is to

be generated

Select File for report to be generated