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0 2 4 6 8 10 12 0 2 4 6 8 10 12 Log 2 ratio (Microarray) L o g 2 r a t i o ( r e a l - t i m e P C R ) y=0.888x + 0.5361 R 2 = 0.9496 -150 -100 -50 0 50 -20 0 20 40 60 0 500 1000 1500 2000 0 200 400 600 0 200 400 600 800 1000 -150 -100 -50 0 50 Fold change 6 12 24 72 Compatible interaction Incompatible interaction (A) CK213159 (B) CN009367 (C) CD869243 (D) BQ295073 (E) CD875175 (F) BQ838257 6 12 24 72 6 12 24 72 A B Time after Hessian fly infestation (h) Figure S3. Validation of microarray data through qPCR. (A) qPCR results of six representative genes with GenBank accession numbers CK213159, CN009367, CD869243, BQ295073, CD875175, and BQ838257. (B) Correlation analysis of microarray and Real-time PCR data sets.

Log 2 ratio (Microarray)

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A. B. (A) CK213159. (B) CN009367. (C) CD869243. y=0.888x + 0.5361 R 2 = 0.9496. Fold change. (E) CD875175. (D) BQ295073. (F) BQ838257. Log 2 ratio (real-time PCR). 6 12 24 72. 6 12 24 72. Time after Hessian fly infestation (h). 6 12 24 72. - PowerPoint PPT Presentation

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Log2 ratio (Microarray)L

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Time after Hessian fly infestation (h)

Figure S3. Validation of microarray data through qPCR. (A) qPCR results of six representative genes with GenBank accession numbers CK213159, CN009367, CD869243, BQ295073, CD875175, and BQ838257. (B) Correlation analysis of microarray and Real-time PCR data sets.