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Linking Multiple Ontologies:
The OBO Foundry ApproachChris Mungall
NIAID Cell Ontology WorkshopMay 2008
Outline
• Introduction to ontologies– The OBO perspective– Case study in the Gene Ontology
• The OBO Foundry: goals and principles• The OBO relation ontology• Organization of ontologies in OBO• Modularity
– An example from CL
• Linking CL to the OBO Foundry
What is an ontology?
• A computable representation of some domain– What kinds of
things exists– What are the
relations that hold between them?
Mitral valve Aortic valve
Heart
Cavitated organCardiovascular
System
part_of part_of
part_ofis_a
Aspects of an ontology
• Identifiers– Uniquely identify a class / term
• E.g. CL:0000037 is ID for the term “hematopoietic stem cell”
– Identifier metadata
• Terminological aspects– Names and synonyms/alternate labels
• CL:0000037 has “hemopoietic progenitor cell” as a related synonym and “hemopoietic stem cell” as exact synonym
• Logical aspects– Relations– Definitions Provenance
Some ontologies and their uses
• The Gene Ontology– Annotation of gene products– Analyzing high-throughput datasets
• Anatomical ontologies (including CL)– Experimental metadata– Image annotation– Indicating location of gene expression– Creating Phenotypic descriptions
• Others– NLP– Annotating information models– Database integration
Origins of OBO: The Gene Ontology (GO)
• 3 ontologies for annotating genes and gene products
• These ontologies are organised as a collection of related terms, constituting nodes in a graph– Gradually incorporating other logical axioms
Ontology # terms # links
Molecular function 7889 9225
Biological process 13978 25065
Cellular component 2034 3894
Annotation and GO
• GO Annotations:– Associations between genes and GO terms, with
evidence– Met17 : “methionine metabolism” GO:0006555
• 222,000 genes and gene products have high quality annotations to GO terms– 3.4m including automated predictions– 66,000 publications curated
• Variety of analysis tools– http://www.geneontology.org/GO.tools.shtml#micro
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GO::TermFinderSherlock et al
GO and high-throughput biology:Over-representation of GO terms for
gene sets
GO and the need for OBO
• GO terms implicitly reference kinds of entities outwith the scope of GO– Methionine biosynthesis– Neural crest cell migration– Cardiac muscle morphogenesis– Regulation of vascular permeability
• OBO was born from the need to create source ontologies for GO term ‘cross-products’– Define composite classes in terms of simpler ones
chemicalcell
anatomyquality
The Open Biomedical Ontologies (OBO) Foundry
• A collection of orthogonal reference ontologies in the biological/biomedical domain
• The OBO Foundry: Each is committed to an agreed upon set of principles governing best practices in ontology development
Some OBO ontologies
• Gene Ontology• ChEBI - chemical entities• OBI - investigations• PATO, MP - phenotypes• CL - cells• ENVO - environment and
habitat• DO - Human diseases• CARO - common anatomy• FMA - human anatomy
• SO - sequence features• Model organism
anatomy– ZFA– Fly_anat– Dicty_anat– Mouse_anat– …
• OBO Relation Ontology
OBO Foundry: criteria, v1
• Open• Well-defined exchange format
E.g. OBO or OWL
• Uses identifiers according to OBO ID policy• Ontology Life-cycle / versioning• Has clearly specified and delineated content• Has unambiguous definitions• Uses or extends relations in the OBO Relation Ontology• Well documented• Has a plurality of users (and a mail list & issue tracker)• Developed collaboratively• Orthogonal, modular
http://obofoundry.org/
OBO Relation Ontology
• Edges can link nodes…– Within ontologies– Across ontologies
• The precise meaning of the relation is important– Relations have formal definitions– Rules for composing relations together
– http://obofoundry.org/ro/
Is_a
• X is_a Y– If something is an instance of X (at
time t), then it is also an instance of Y (at t)
• Transitive– B1 B cell is_a B cell– B cell is_a lymphocyte– Therefore B1 B cell is_a lymphocyte
Part_of
• Instance level part_of relation is primitive• Between classes:
– X part_of Y :• Every instance of X is part_of some instance of Y• Paneth cell part_of intestine : YES• Nucleus part_of Cell : YES• Neuron part_of brain : NO
– (there are some neurons that are part of others parts of the nervous system)
• Transitive– X part_of Y, Y part_of Z
• Therefore, X part_of Z
Has_part
• Instance level inverse of part_of• X has_part Y
– Every X has some Y as part– Cell has_part nucleus : NO– Nucleate erythrocyte has_part
nucleus : YES
Develops_from
• X develops_from Y– Every instance of X was once a Y, or inherited a
significant portion of its matter from a Y• Example: erythrocyte develops_from reticulocyte
• Transitive– erythrocyte develops_from reticulocyte– reticulocyte develops_from orthochromatic
erythroblast• =>
– erythrocyte develops_from orthochromatic erythroblast
Transformation and derivation
• Develops_from relation can be refined into two cases:– Transformation_of
• X transformation_of Y :– Any instance of X was previously an instance of Y– Example: erythrocyte transformation_of reticulocyte
– Derives_from• X derives_from Y :
– Holds between distinct instances where Y inherits matter from X
• Most OBO ontologies just use the develops_from relation
Other relations
• Inherence– Between a quality and an object– E.g. between a specific shape and a
cell
• Participation– Between a process and an object– E.g. between a B cell and an immune
process
Definitions state necessary and sufficient
conditions• Links in the ontology graph state necessary
conditions for a class• E.g. erythroid progenitor cell develops_from
megakaryocyte erythroid progenitor
– These characteristics may not be unique
• A definition should state necessary and sufficient conditions for a class– The characteristics must be unique to the defined class
• E.g. “progenitor cell that is committed to the erythroid lineage”
• Definition should be precise and (as far as possible) translated / translatable to logical computable form
Genus differentia definitions
• Of the form– An X is a G that D– G should be in the same ontology– D is discriminating characteristics that
differentiate (in the classification sense) Xs from other Gs.
• Relations to terms in an ontology (the same ontology or a different one)
• Example:– A B cell is a lymphocyte that expresses an
immunoglubulin complex
Orthogonality of ontologies
• No two ontologies should represent the same kind of entity– E.g. “B-cell” should only be represented in one
ontology– Related entities should be coordinated across
ontologies• GO: “B-cell differentiation”
• Exceptions:– The term “cell” connects GO Cellular Component (cell
parts) and CL (cells)
• Advantages:– Reduces redundancy and work– Easier to make the union consistent
oenocyte
hepatocyte
liverfat body
glycogenglucose
hepaticartery
bile
insulin
obesity
carbohydratemetabolism
liverdevelopment
increased circulating glucose level
oenocytedifferentiationhepatoma
Some OBO terms..
oenocyte
hepatocyte
liverfat body
glycogenglucose
hepaticartery
bile
insulin
obesity
carbohydratemetabolism
liverdevelopment
increased circulating glucose levelCHEBI
FBbt
CLPRO
MA(mouse)(fly)
FMA(adulthuman)
MP(mammalphenotype)
GO(biologicalprocess)
oenocytedifferentiationhepatoma
DO
oenocyte
hepatocyte
liverfat body
glycogenglucose
hepaticartery
bile
insulin
obesity
carbohydratemetabolism
liverdevelopment
increased circulating glucose levelCHEBI
FBbt
CLPRO
MA(mouse)(fly)
FMA(adulthuman)
MP(mammalphenotype)
GO(biologicalprocess)
oenocytedifferentiationhepatoma
DO
oenocyte
hepatocyte
liverfat body
glycogenglucose
hepaticartery
bile
insulin
obesity
carbohydratemetabolism
liverdevelopment
increased circulating glucose levelCHEBI
FBbt
CLPRO
MA(mouse)(fly)
FMA(adulthuman)
MP(mammalphenotype)
GO(biologicalprocess)
oenocytedifferentiationhepatoma
DO
How should we organize this?
Top-level organisation (BFO: Basic Formal
Ontology)• General categories– 3D things (continuants)
• Independent– Cells, organs,
molecules• Dependent
– Shapes, sizes, concentrations, …
– 4D things (processes)• Processes
• Useful organisational principle for OBO
• is_a and part_of should not cross top level categories
• Levels of granularity (scale)– Population– Organism– Organ– Cell– Molecule
• part_of relations can cross levels
oenocyte
hepatocyte
liverfat body
glycogenglucose
hepaticartery
bile
insulin
obesity
carbohydratemetabolism
liverdevelopment
increased circulating glucose levelCHEBI
FBbt
CLPRO
MA(mouse)(fly)
FMA(adulthuman)
MP(mammalphenotype)
GO(biologicalprocess)
oenocytedifferentiationhepatoma
DO
Objects Qualities etc Processes
CONTINUANT OCCURRENT RELATION TO
TIME GRANULARITY INDEPENDENT DEPENDENT
ORGAN AND ORGANISM
Organism (NCBI
Taxonomy)
Anatomical Entity (FMA, CARO)
Organ Function (FMP, CPRO)
Organism-Level Process
(GO)
CELL AND CELLULAR
COMPONENT
Cell (CL)
Cellular Component (FMA,GO)
Cellular Function
(GO)
Phenotypic Quality (PaTO)
Cellular Process (GO)
MOLECULE Molecule
(ChEBI, SO, RnaO, PrO)
Molecular Function (GO)
Molecular Process (GO)
The OBO Foundry can help with modular ontology
design• Biology is complex
– So our ontologies will be complex– Multiple purposes– Multiple means of classifying
• Separate out different aspects– Modular approach– Avoid multiple inheritance (>1 is_a parent)
• Don’t over-use is_a• Don’t cross aspects with is_a
• Make complex descriptions from simpler parts– Polyhierarchies arise from composition
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Cysteine biosynthesis(trimmed)GO
Tangled polyhierarchy
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Cysteine biosynthesis(trimmed)
Process axis
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Cysteine biosynthesis(trimmed)
Chemical structure axis
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Cysteine biosynthesis(trimmed)
ChEBI(trimmed)
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Cysteine biosynthesis(trimmed)
ChEBI(trimmed)
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Cysteine biosynthesis(trimmed)
ChEBI(trimmed)
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Cysteine biosynthesis(trimmed)
ChEBI(trimmed)
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We can do more than simply link terms:
Cross-products (aka logical definitions,Computable genus-differentia definitions)
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Cysteine biosynthesis(trimmed)
ChEBI(trimmed)
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Cysteine biosynthesisGO:0019344
=
a biosynthetic process GO:0009058that
results_in_creation_of cysteine CHEBI:13536
} genus
differentia}
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Cysteine biosynthesitic process = biosynthetic process that results_in_change_to cysteine
results_in_change_to
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Let the computerdo the work..
Given cross-products,A reasoner can addall links
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Underlying representation is normalized
Example of is_a-overloading: OBO Cell
Ontology(current)
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CL
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•Try not to assert too many is_a parents
X
CL
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•Reuse existing ontologies•Non-is_a relation
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?
CL GO
Hasfunction
How CL can use other OBO ontologies
• GO Cellular component– Mononuclear phagocyte– B cell (expresses immunoglubulin complex)
• GO Biological process– Photosynthetic cell
• PATO Qualities– Spiny neuron
• CHEBI Chemical entities– X secreting cell
• Anatomy Ontologies– CNS neuron
Molecular function, PRO - CD4 positive cell
How CL is used by other ontologies
Ontology Example Genus Differentia
GO-BP T cell differentiation
Cell differentiation
Results_in_acquisition_of_features_of
T cell
GO-CC Germ cell nucleus Nucleus Part_of
germ cell
MP Abnormal macrophage morphology
Abnormal morphology
Inheres_in
macrophage
ZFA (zebrafish)
erythrocyte erythrocyte In_organism DanioHas_part nucleus
OBI
DO (disease)
Ontology Example Relationship
Fly anatomy R8 photoreceptor cell
Part_of ommatidium
Results
• Biological process x CL• http://wiki.geneontology.org/index.php?XP:biologi
cal_process_xp_cell
– Uncovered inconsistencies between GO and CL
– Oenocyte differentiation is_a columnar/cuboidal epithelial cell differentiation
• MP x CL• http://wiki.geneontology.org/index.php/XP:mamm
alian_phenotype_xp
– Resulted in various fixes to MP
OBD: Ontology Annotation Database
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Summary
• The cell ontology is a representation of the types of cell that exist
• The OBO Foundry provides– Principles– A framework for connecting ontologies
• There are many points of coordination between CL and other OBO ontologies
• CL could benefit from the gradual introduction of a modular approach
The Gene Ontology; and beyond
• Curation of genes and gene products– Molecular function– Biological process– Cellular component
GO
Multiple databases using the same ontology
The Gene Ontology; and beyond
• Curation of genes and gene products– Molecular function– Biological process– Cellular component
• What about curation of other data types?– Expression, transcriptomics– Genetics, phenotypes and
disease– Many others..
• OBO– Open Bio-Ontologies– Arose partly in response to
requirements outside scope of GO
GO
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Islands of biological data
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GOAnatomyontologies Phenotype
ontologies
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Connecting the islands
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Connecting the islands
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Bada et al : GO to ChEBI
http://www.berkeleybop.org/obol
Amino acid cross-products in GO:
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http://www.berkeleybop.org/obol
• GO approach is retrospective– Text based approaches to ‘decompose’ terms
• Obol• Bada/Hunter
– Born of necessity• OBO did not exist when GO started
– Hard work
• New ontologies should take the prospective approach– Separate out aspects from the outset– No heuristic parsing necessary
Prospective approach: Sequence Ontology
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Separate hierarchies created from the outset- cross-products made from the beginning
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OBI: Ontology for Biomedical Investigations
• Successor to MGED/FuGO• Represents the realm of investigations
– Biomaterials– Equipment– Protocols– Data transformations
• Makes maximal use of OBO– PATO:– ChEBI:
• Primary representation language is OWL– Uses OWL translations at http://purl.org/obo/
Social Insect Behavior Ontology
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• 4 distinct hierarchies– Anatomical entity– Behavior– Chemical entity– Species
• Links– derives_from, between
chemical and anatomical entity
• Future plans– Submit chemical
terms to ChEBI– Upper level behavior
ontology?
Anatomy
• GO is relevant for all kingdoms of life• Development of anatomical ontologies has
been less coordinated– Cell & subcellular: one ontology applicable to all– Gross Anatomy: multiple ontologies
• Vertebrate:– MA + EMAP: Mouse– FMA: Human (adult)– EHDA: Human– ZFA: Zebrafish– TAO: teleost anatomy– XAO: Xenopus
•Invertebrate:–FBbt: Drosophila anatomy–Tick anatomy–Mosquito anatomy
Anatomy: Ongoing work
• CARO– Upper level shared anatomical ontology– Very general terms
• Teleost anatomy ontology– Broader than zebrafish anatomy ontology– Will include homology links
• Linking cells to gross anatomical entity– Purkinje cell part_of cerebellum– Spans ontologies (CL + ssAO)
• BIRNLex• Stages and development
poster
poster
poster
talk
Using multiple ontologies: Pre vs post composition
• Complex descriptions (aka cross-products) can be composed from 2 or more terms– By ontology editors (pre)– By curators (post)
• Example:– Liver hyperplasia
• Precomposed phenotype ontology– MP:0005141 “liver hyperplasia” increased size of liver due to
increased hepatocyte cell number
• Post-composition at time of genotype curation– PATO:0000644 “hyperplastic”– MA:0000358 “liver”
• Which strategy to choose?
• Either strategy can be used• Or mixed and matched
– Caveat:• Pre-composed terms must have computable
definitions (cross-products)• Currently created retrospectively
• Current progress : – MP (Mammalian Phenotype):
• 4136/5760 xp defs, partially vetted• Caveat: species-specificity
– WormPhenotype:• 350/1569 xp defs
– PlantTrait:• 340/765 xp defs, partially vetted
Other ontologies
• Envo + GAZ– Environmental ontology and gazetteer– Habitats:
• Host (anatomy)• Geographical features (eg hydrothermal vents)
– Qualities, chemical entities
• BIRNLex• Protein Ontology
– Links to/from GO• Complexes• Functions of ancestral proteins
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Envo-based annotation in Phenote
Technical consequences of modular approach
• Dependencies– Technical issues
• Dependence on network?• Formats - converters
– Social & management issues– Change and versioning
• http://www.bioontologies.org/
• Managing dependencies• http://obofoundry.org/wiki/index.php/Mappings
– Stable URLs for downloading ontologies in obo or owl http://purl.org/obo/
– OBO Identifier policy• http://obofoundry.org/wiki/index.php/Identifiers
Conclusions
• Be modular– Distinct hierarchies– Avoid is_a overloading– Link to existing ontologies
• Rewards– Standards– Increases value of curated data– Reduces duplication of effort and maximises
curation effort– Ontologies are long term infrastructure
• It’s worth getting them right
Learning more
• http://www.bioontology.org– National Center for Biomedical Ontology– Browse and search OBO– Coming soon: inter-ontology links
• http://obofoundry.org– Principles and recommendations– Participation
• Mailing lists• Trackers
Restructuring Cell.obo
OBO Cell Ontology
• Current version– Overloading of is_a hierarchy– Difficult to maintain– Leads to “true path” violations
• Refactoring– Replace is links with has_function– Keep main axis structure-based (but not religiously
so)
• For every term immediately under cell-by-function, we made a new function term
• propagation of genome • to circulate• to secrete• to metabolise• to contract• Electrical absorption• Barrier• Motility• Structural• to accumulate stuff• signaling (mitogenic)• to die• Defense• Transport• to photosynthesize• to support• Valve• to fix nitrogen
• Also create grouping terms
• Replaced is_a links to cell-by-function terms with has_function links to corresponding function terms
• What do we do about the old cell-by-function terms?
• We can eliminate them..
• OR we can support them, but infer the ‘tangled DAG’
• Requires xp defs:– Nitrogen fixing cell = cell THAT has_function nitrogen-fixing
• Future work / ongoing issues:
• Redundancy between cell functions & GO biological process?
• Cell-by-lineage
Synchronizing ssAOs and CL
• Fly_anat, zfa, plant_anat all represent cell types– Part_of links from cells to gross anatomy
• E.g. purkinje_cell part_of cerebellum
• Methodology– Xrefs from ssAOs to CL IDs– Treat as ss subtypes– Use reasoner to stay in sync– http://www.bioontology.org/wiki/index.php/CL:Alignin
g_species-specific_anatomy_ontologies_with_CL– Examples:
• http://www.berkeleybop.org/obol/#fly_anatomy_xp_cell-obol
Transformation_of
• Class-level relation between continuant types• Transitive
• Relation between two classes, in which instances retain their identity yet change their classification by virtue of some kind of transformation. Formally: C transformation_of C' if and only if given any c and any t, if c instantiates C at time t, then for some t', c instantiates C' at t' and t' earlier t, and there is no t2 such that c instantiates C at t2 and c instantiates C' at t2
Derives_from
• Holds between continuants• transitive
• Derivation on the instance level (*derives_from*) holds between distinct material continuants when one succeeds the other across a temporal divide in such a way that at least a biologically significant portion of the matter of the earlier continuant is inherited by the later
• We say that one class C derives_from class C' if instances of C are connected to instances of C' via some chain of instance-level derivation relations.
• Examples:– osteocyte derives_from osteoblast
CONTINUANT OCCURRENT RELATION TO
TIME GRANULARITY INDEPENDENT DEPENDENT
ORGAN AND ORGANISM
Organism (NCBI
Taxonomy)
Anatomical Entity (FMA, CARO)
Organ Function (FMP, CPRO)
Organism-Level Process
(GO)
CELL AND CELLULAR
COMPONENT
Cell (CL)
Cellular Component (FMA,GO)
Cellular Function
(GO)
Phenotypic Quality (PaTO)
Cellular Process (GO)
MOLECULE Molecule
(ChEBI, SO, RnaO, PrO)
Molecular Function (GO)
Molecular Process (GO)