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Your Innovation Partner in Drug Discovery!
GenScriptA World-leading Biology CRO
Howard HuangRegional Business Manager for New England
About GenScript
Founded in 2002
More than 1,000 employees
Headquarters in Piscataway, NJ, USA
Facilities and branches:
New Jersey, USA Nanjing, China
Paris, France
Tokyo, Japan
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Key Facts
One of the world-leading biology Contract Research Organization
The largest gene synthesis supplier in the world
The provider of custom protein, peptide, antibody, and cell line services with the best guarantee in the industry
Drug discovery platform including assay and screening, antibody drug development, bioprocess, structure biology, and animal model
ISO9001:2008 and AAALAC certificated
24-hour customer support
Global presence with subsidiaries in Europe and Asia, and major operations in China with advanced facility and >108,000 ft2 lab space
Customers include major biotech firms, research institutions and the world's top pharmaceutical companies in over 70 countries
Seasoned science and business management team
3Your Innovation Partner in Drug Discovery!
Fast Growing Company
4Your Innovation Partner in Drug Discovery!
Our Organic Growth Our Customers
May 2009:GenScript gets VC from Kleiner Perkins Caufield & Byers (KPCB)
Investors
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Most respected venture capital firm in the world Funded Genentech, Google, Amazon, Pacific Biosciences, etc Invested in > 300 IT , life science, and biotech companies
6Your Innovation Partner in Drug Discovery!
The new site spans on 100 acres of land. 7 buildings with 300,000 square feet of floor plan will function as
offices, R&D, production, and experimental animal facilities. Capacity expansion:
Gene synthesis: Beyond 5,000 kb per month Protein expression: More than 300 batches per month Antibody: More than 300 custom orders per month,
maintenance of 2,000 catalog products Bioprocess: GLP grade bioproduction
New Biology Facility in Jiangning, Nanjing
7Your Innovation Partner in Drug Discovery!
New Peptide Facility in Pukou, Nanjing
27,000 square feet of floor plan will function as research and production facilities
Capacity expansion: Standard peptide synthesis: 6,000
peptides per month
Peptide library: up to 50,000 peptides per month
Maintenance of more than 2,000 peptide products
8Your Innovation Partner in Drug Discovery!
Drug Discovery Outsourcing Becomes Trend
9Your Innovation Partner in Drug Discovery!
10Your Innovation Partner in Drug Discovery!
The bottleneck in early drug discovery
• The decoding of the human genome and the rapid pace of biological research have generated numerous potential drug targets.
• Assay Development and building screening cascades now become the limiting factors in early drug discovery.
• Outsourcing has already been shown to dramatically increase the capacity of medicinal chemistry.
Drug Targets
Small / Big molecule
High Affinity compound
Drug
Assay
11Your Innovation Partner in Drug Discovery!
Bottleneck: Bio-reagent Preparation
Typical delay caused by reagent preparation time for a program is anywhere from 3-12 months.
Some programs simply cannot be initiated at all due to un-availability of appropriate validated bio-reagents.
Assay Design
(3-12 months)
Bio-Reagent
Preparation and Assay
Setup
(6-12 months)
Screening
(2-4 months)
Target Identification
Target Validation
Assay Development & Screening
LeadOptimization
Preclinical Drug Candidate
Bio-reagent Center
Bio-Assay Center
Lead Optimiztion Center
12Your Innovation Partner in Drug Discovery!
GenScript: Removing the bottleneck
GenScript is providing a total solution from custom bio-reagent, assay development and screening for early drug discovery.
GenScript Factory: Assembly-Line
13Your Innovation Partner in Drug Discovery!
Laboratory: Same person for different steps, uncontinuable mode
GenScript Factory: Dedicated person for each position, continuable mode
Biology Research Services
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Our Biology Contract Research Services
Gene Synthesis
— Any gene you desire!Synthesize genesOptimumGeneTM Codon OptimizationLarge-Scale Gene Synthesis
Cloning
—Any construct you want to build! PCR Cloning/SubcloningGenPoolTM ORF CloningKI & KO Vector ConstructionCustom vector construction
Mutagenesis
— Any variant from your design!Mutagenesis Service Site-Directed Mutagenesis LibrariesSequential Permutation Scanning LibrariesRandomized and Degenerated Libraries
Plasmid Preparation
— Any amount to fit your plan!Flexible Scale: 100 μg to 1,000 mgLow Endotoxin Levels: <5 EU/mg
Largest Gene Synthesis Supplier in the World
Transforming Molecular Biology
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16Your Innovation Partner in Drug Discovery!
OptimumGeneTM - Codon Optimization
GenScript OptimumGeneTM Gene Design system is a proprietary gene optimization technology that can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of productivity in any given expression system.
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OptimumGeneTM – Cases Study
Fig. α. Expression Result of Protein α after Gene Optimization. The expression level of Protein A using GenScript’s OptimumGeneTM optimization is 3 times more than that of competitor’s.
Fig. β. Expression Result of Protein β after Gene Optimization. The expression level of Protein B using GenScript’s OptimumGeneTM optimization is 13 times more than that of competitor’s.
One-Stop Protein Service
19Your Innovation Partner in Drug Discovery!
20Your Innovation Partner in Drug Discovery!
21Your Innovation Partner in Drug Discovery!
DHFR CHO expression system
No patent or IP issue
Research grade stable cell line (12-14 weeks)
Transient expression evaluation
Transfection and screening
Stability study and delivery of top 3 clones
Production grade stable cell line (20-24 weeks)
Include all work for research cell line creation
Plus 3 rounds of MTX amplification and subcloning of top 3 clones
Other stable mammalian cell lines upon request
22Your Innovative Partner in Drug Discovery!
Stable Gene Expression
Custom Peptide Synthesis Services
Custom Peptide Synthesis Services
Standard Peptide Synthesis
High complexity and purityLong peptides up to 200 aaHigh success rate ≥ 95%
Peptide Libraries
Rapid parallel platformCompetitive prices Guaranteed deliveryFlexible screening tools
Cosmetic Peptides
FlexPeptideTM technologyComprehensive documents
Batch size from mg to 2 kgPeptides up to 50 aa
Recombinant Peptides
Flexible technologyVariety of modifications Any length, any sequence, any scale
Non-GMP Peptides
GMP Peptides
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Leading the industry standard on peptide success rate …
pAb Services
Affinity-Purified pAbPhospho-Specific pAb
Express AntibodyTM ServicesSpecies: rabbit, goat/sheep, chicken, mouse, rat, etc
mAb services
MonoExpressTM Antibody Services mAb Production ServicesMost Popular: Phospho-Specific pAb Package
Large Scale Antibody Production Services
Additional Services
Ab ModificationAb PurificationAb CharacterizationCell Line StoragePeptide Synthesis Services
Immunoassay Development Services for Drug Discovery & Clinical Studies
Guaranteed Antibody Services
Custom pAb and mAb ServicesCatalog antibodiesExpress servicesBest guaranty in the industry
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Leading the industry standard on antibody guarantee …
Best guarantees in the Industry For Antibody product generated in any host using any qualified antigen,
we guarantee ELISA titer 1:32,000 or better.
For Monoclonal Antibody product generated in any host using any qualified antigen, we guarantee two positive clones.
For both Polyclonal and Monoclonal antibody product generated using qualified protein antigen, we guarantee positive Western blot results.
25Your Innovation Partner in Drug Discovery!
Antibody Services
26Your Innovation Partner in Drug Discovery!
Given a target, we will delivery HTS-ready assays to you
GPCR Assay Services
• Custom GPCR Stable Cell Line Services • Cell-Based Assay Development • Compound Primary & Secondary Screening • GPCR Profiling
Ion Channel Assay Services
• Custom Ion Channel Services• Functional Profiling Assays• Compound Screening
Enzyme Assay Services
• Kinase & Protease Assay Development• Kinase & Protease Compound Screening• Kinase & Protease Compound Profiling• Other Enzyme Assays
One-Stop Assay Services
• Target-to-Assay Solutions• Assay Reagent Preparation• HTS-ready formatting• Compound profiling
Seamless Bio-Assay Center
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28Your Innovation Partner in Drug Discovery!
Assay dev and screening
GPCR
Ion channel
Enzyme• Kinase
• Protease
• Other
Nuclear receptor
Transport
More
Oncology drug discovery
In vitro• Proliferation
• Colony formation
• Cytotoxicity
• Cell adhesion
• Migration & invasion
• Angiopoiesis
• Apoptosis
• Cell death
• Genotyping
• RNAi
In vivo• Animal efficacy
• PK
29Your Innovation Partner in Drug Discovery!
Antibody drug discovery
ADCC CDC BiaCore SPR ELISA TNF-alpha
induced cytotoxicity
Safety
assessment
Cardiotoxicity hERG binding hERG patch clamp hKv1.5 hNav1.5 Cav1.3
Drug interactions Heptotoxicity HepG2 inhibition Gentoxicity Mitochondrial
toxicity
Antibody Drug Development Center
Your One-Store Shop for Success
Innovation & Technology
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Gene-on-Demand TM gene synthesis technology
- best and most economic gene synthesis
FlexChem TM ligation technology
- peptide synthesis up to 200 amino acids
CloneEZ TM seamless cloning technology
- state-of-art cloning into any vector at any site
Nanotechnology for adjuvant mixing
- induction of enhanced immune response
Diversified expression systems
- bacteria, yeast, insect cells, and mammalian cells
T-Max TM adjuvant
- increased titer of at least ten times, and reduced
cost
FoldingArt TM technology
- refold rate of 95% for inclusion bodies and
proteins (virus expression)
OptimumAntigen TM antigen design
- optimized antigen design
293TGE TM system
- most efficient transient expression system
OptimumGene TM codon optimization technology
- optimized gene expression
Innovative technologies for enhanced services
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Full IP Protection
A secure web-enabled resource to list the program progress/reagent availability for each client.
Your Innovation Partner in Drug Discovery!
Business Models
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Your Innovation Partner in Drug Discovery! 34
Thank You!
Howard Huang
Regional Business ManagerNew England
