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www.drugtargetreview.com/workshops
Brought to you by the Fraunhofer Institute for Molecular Biology and Applied Ecology IME
a Drug Target Review workshop
CELL-BASED ASSAYSFOR SCREENING 22-24 November 2016Hamburg, Germany
NEW TOPICS INCLUDING
Cell-based and iPS-based screening,
DropTech and stem cell
technology
LIMITED
PLACES REMAINING
BOOK NOW!
Iin assocation with Sponsored by
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WORKSHOP INSTRUCTORS:
Dr. Sheraz Gul is Head of Assay Development & Screening at Fraunhofer-IME SP, Hamburg. He has worked in Big Pharma and academia and has experience in developing biochemical and cellular assays for High Throughput Screening and his research interests are directed towards maximising the impact of HTS for drug discovery.E-mail: [email protected]
Oliver Keminer is a drug discovery scientist with 15 years of experience whilst working at Eli Lilly (Lilly Forschungs GmbH, Hamburg), Evotec and European ScreeningPort GmbH. He has a particular expertise in HCS and holds a diploma in biology from the Ruhr-University of Bochum.E-mail: [email protected]
www.drugtargetreview.com/workshops
WORKSHOP OVERVIEW:
Drug Target Review workshops are limited to 20 attendees to ensure every participant receives the training they require. We continually update our programme to include the very latest screening technologies, ensuring attendees receive the most up-to-date hands-on training and information.
Taking place over 3 days, the workshop offers a series of practical sessions and lectures to examine the design and application of cell-based assays for small molecule screening campaigns in drug discovery. In the practical sessions, participants will have the chance to develop screening-compatible cell-based assays, take part in primary screening using a small molecule library, and profile compounds in dose-response experiments.
Attendees gain insight into the key parameters to be considered when developing cell-based assays and performing small molecule screening campaigns, associated data analysis, validation of Hits and their annotation using a variety of cell health/toxicity/liability assays.
The evening dinner on the first day will offer the opportunity for the participants to network and establish relationships that would be mutually beneficial.
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www.drugtargetreview.com/workshops
FURTHER DETAILS:
A typical small molecule drug discovery project aims to identify chemical starting points that modify the functions of genes, cells, or biochemical pathways ultimately leading to new therapeutics [1]. Screening using miniaturised microtiter plate formats remains the most widely utilised methodology for identifying novel chemical starting points that are capable of modulating target function in a meaningful, biologically relevant manner [2]. The initial steps in small molecule drug discovery include the selection of a target, development of an assay to monitor its activity, and its screening against a compound library. Subsequently, the Primary Hits are optimised using multiple criteria including structure activity relationships, selectivity, physicochemical properties and liability [3,4] that could result in a Lead compound series.
We are now witnessing a resurgence of cell-based assays including phenotypic assays where the underlying target/s are unknown [5] and advances in cell-based assays are also being made, for example using human induced pluripotent stem (iPS) cell-derived cells that better recapitulate normal human biology compared to transformed cell lines and non-human primary cells [6].
1. Goodman M. Market watch: Pharma industry performance metrics: 2007-2012E.
2. Macarron R, et al. Impact of high-throughput screening in biomedical research. Nat Rev Drug Discov 2011;10:188.
3. Bleicher KH, et al. Hit and lead generation: beyond high-throughput screening. Nat Rev Drug Discov 2003;2:369.
4. Gul S. Overview of the gene-to-Lead phase in drug discovery. Eur Pharm Rev Digital 2009;6:3.
5. Swinney DC and Anthony J. How were new medicines discovered? Nat Rev Drug Discov 2011;10:507.
6. Björn Brändl, Bernhard M. Schuldt, Lena Böhnke, Oliver Keminer, Lea A. I. Vaas, Rainer Fischer, Franz-Josef Müller, Ole Pless. Utilizing Regulatory Networks for Pluripotency Assessment in Stem Cells. In print Current Stem Cell Reports (Springer)
REFERENCES
02
Introductions
Literature Review
Break and Lab Tour
Talk: High Content Screening (HCS) in Translational Drug Discovery
Case study: Image Analysis in Columbus
Introduction to the Thermo Scientific Multidrop™ Combi reagent dispenser
Lunch
Overview of practical work for Day 1
Introduction to Thermo Scientific Multidrop™ Varioskan™ LUX multimode reader
Experimental work, data analysis and preparation of presentations
Dinner. (evening finishes at 21:00)
09:00
DAY 1
DAY 2
DAY 3
09:00
09:30
10.30
11:15
11.45
12.15
12:30
13:30
Discuss results from Day 1
Talk from DiscoverX
Case Study (kinase target)
Break
iPS Cell-Based Assays
Overview of practical work for Day 2
Lunch
Experimental work, data analysis and preparation of presentations (Day finishes at 18:00)
09:45
11.00
10:00
11:15
11:45
14:00
09:00
10:00
11:30
11.00
12:30
13:30
14:00
Programme
12:30
14:15
Discuss results from Day 2
Case Study (enzyme target)
Break
Case Study (nuclear receptor)
Lunch
Talk: DropTech® screening platform
Teams to present results and identify learnings from workshop
Complete Feedback Forms. (Day finishes at 15:00)
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18:30
13:30
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The workshop is designed for scientists at all levels (undergraduates, postgraduates and laboratory based scientists within academic and industrial research organisations) engaged in early stage drug discovery and have an interest in the development, validation and utilisation of cell-based assays for screening against small molecule libraries.
The workshop is equally well suited to technically focused staff from core facilities or contract research organisations who may wish to extend their expertise.
WHO ATTENDS THIS WORKSHOP?
www.drugtargetreview.com/workshops
ADDITIONAL BENEFITSApproved by the Society of Biology for purposes of Continuing Professional Development (CPD), the cell-based assays for screening workshop may be counted as 72 CPD credits. Please note that these credits are only valid if attendees are registered on the Society of Biology CPD Scheme.
n Compound handling (from manual to Labcyte ECHO) n Reagent dispensing (Thermo Multidrop™, Tecan Fluent™)n Cell viability (fresh and frozen cells) and apostosis (caspase 6) assays n HDAC enzyme assays (Tecan Fluent™ & Infinite F500)n DiscoverX PathHunter® kinase assay n Luminesence readout (PerkinElmer EnVision® and Thermo Varioskan™) n High Content Screening (HCS) cardiomyocyte imaging (PerkinElmer Opera and Sony SI8000)n Live-content imaging (PerkinElmer EnSight™ and Essen IncuCyte®)n Image analysis using PerkinElmer Columbus™n Monitoring cardiomyocyte beating as toxicity readout (IBMT Contraction Reader)n Thermo Scientific Muitidrop combireagent dispensen Thermo Scientific Muitidrop Varioskan Lux multimode readern PathHunter® eXpress OPRD1 CHO-K1 b-Arrestin GPCR Assay
EQUIPMENT AVAILABLE TO USE DURING THE PRACTICAL SESSIONS:
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www.drugtargetreview.com/workshops
THE EQUIPMENT
During this year’s workshop, the Thermo Scientific Multidrop™ Combi reagent dispenser and Varioskan™ LUX multimode reader will be used to help prepare and analyse samples in the practical sessions of the Cell-Based Assays for Screening Workshop.
The Multidrop Combi dispenser can quickly and precisely fill plates with buffers, enzymes and substrates. This compact dispenser features an autoclavable 8-channel dispensing cassette, and supports a wide range of plate types and volume ranges.
The Thermo Scientific Varioskan LUX Multimode Microplate Reader is designed for bioscience researchers with a wide variety of needs. It comes equipped with a flexible range of measurement technologies including Absorbance and Fluorescence, with optional Luminescence, AlphaScreen, and Time-Resolved Fluorescence modules.
The instrument speeds up measurement setup with the automatic dynamic range selection ensuring high sensitivity. The smart safety controls ease workflow and help avoid experimental errors wasting reagents, samples, and time. Varioskan LUX raises the bar for reliability and ease, with technology that works in researcher’s best interest.
DiscoverX’s portfolio of GPCR cell based-assays save you several months of assay development time with the world’s largest collection of stable cell lines to expedite screening and optimization of small molecule and biologic drugs. Identify more biologically relevant hits during your screen with a target-specific, functional and sensitive response from these cell-based assays. Monitor multiple GPCR pathways to identify the best lead molecule with over 650 cell lines that measure calcium, cAMP, β-Arrestin & GPCR internalization. Quickly implement these assays into your screening program with a highly scalable and automation-friendly, add-and-read protocol. With over 1000 publications, the GPCR cell-based assays from DiscoverX are preferred by thousands of researchers globally and was recently voted the “most effective screening strategy” by researchers like you.”
OTHER EQUIPMENT AVAILABLE TO USE DURING THE PRACTICAL SESSIONS:
Included in the package:
n 3 days of lectures and practical workshopsn Delegate packn Accommodation (bed & breakfast)n Refreshments and lunchn Dinner on night onen All attendees will receive a FREE copy of
‘Human Drug Targets: A Compendium for Pharmaceutical Discovery’ By Edward D. Zanders (RRP £135)
This year’s Cell-based Assays for Screening Workshop will take place at the Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Hamburg, Germany.
For directions please visit >
www.drugtargetreview.com/events/cell-based-assays-screening-workshop
VENUE:
Accommodation is included in the cost to attend.n The hotel has been booked for three nights (Monday 21, Tuesday 22 and
Wednesday 23 November) with departure from the hotel on Thursday 24 November
n Entry to the hotel is available from 3pm on arrival and check-out is by midday on your day of departure
n The hotel includes a buffet breakfast and free WIFIn Further information can be found at:
www.drugtargetreview.com/workshops
ACCOMMODATION:
DETAILS
THE PACKAGE:
You will be able to collect your badge and delegate pack from the registration desk at the entrance of the Fraunhofer IME. Registration will be open from 08:30 in the morning of the workshop.
Please note, delegate badges will not be mailed or emailed in advance
YOUR BADGE:
With Accommodation
STANDARD RATE
Without Accommodation
STANDARD RATE ACADEMIC RATE
€1,800 +VAT
€1,500 +VAT
β€1,500 +VAT
€1,200 +VAT
ACADEMIC RATE
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Academia Association Biotechnology Consultancy
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Terms and Conditions. By proceeding with this registration you are committing to placing an order to attend the Workshop(s) at the charge shown above. Payment must be received before the event in order to secure your place. Cancellations must be in writing to [email protected]. Cancellations received 28 days before the event will be subject to an administra-tion charge of 25% of the registration fee. It is regretted that cancellations made after this time will have to pay the full registration fee. Substitutions may be made at any time. If the event is postponed, cancelled or changed, Fraunhofer is not liable for any costs incurred by delegates in connection with their attendance. No refunds of the registration fee will be paid if events outside the control of Fraunhofer, such as transport disruption, weather, terrorism or any other incidents of force majeure occur leading to the cancellation or disruption of the Workshop. VAT: Under EU VAT regulations guests are required to pay VAT at the prevailing
local rate on any event taking place within the EU. Guests may be entitled to reclaim this cost, which can make a sig-nificant reduction on your attendance costs. If you require help with this please contact us. Information you supply may be used by Fraunhofer (and their agent Russell Publishing) for publication (where you provide details for inclusion in our directories or catalogues and on our websites) and also to provide you with information about our products or services in the form of direct marketing activity by phone, fax, email or post. Information may also be made available to 3rd parties on a list lease or list rental basis for the purpose of direct marketing. If at any time you no longer wish to receive anything from Fraunhofer or to have your data made available to 3rd parties, please write to the Data Coordinator, Fraunhofer, Court Lodge, Hogtrough Hill, Brasted, Kent TN16 1NU.
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AREA OF INTEREST (tick all that apply)
CELL-BASED ASSAYS FOR SCREENING WORKSHOP (22-24 November 2016)
Analysis,”HPLC/LC,Mass Spec,Microbiology,NIR,Raman” Assays,”Cellular,Biochemical,Assay Development,Label Free,Microfluidics” Drug Discovery Processes,”Target Validation, Hit-to-lead, Lead optimisation,ADME/TOX/AD-MET,Product Development,Toxicology,”
Genomics,”RNA,MicroRNA,SIrna,miRNA,RNA interference,RNAi” Informatics,”ELN, LIMS, Big Data, Data Visualisation, in silico Predicitve tools, Data Management”
Screening,”High Throughput Screening, High Content Screening, High Content Analysis,Flow Cytometry,Molecular Imaging,Protein Expression”
Sequencing,”Next Generation Sequencing, PCR, dPCR, qPCR,RT-PCR” Stem Cells,”Embryonic, Induced Pluripotent, Pluripotent Adult Stem Cells” Targets,”GPCR’s,Enzymes,Ion Channels,Proteases,Antibodies” Translational Science,”Biomarkers, Personalised Medicine”
DATE:TERMS & CONDITIONS: (Mandatory) Please tick the box to agree to our Terms & Conditions
An invoice will be issued to you by Fraunhofer within two weeks of Russell Publishing Ltd receiving your completed registration form. Settlement must be made within 12 days of receipt of this invoice.
CONFIRM YOUR BOOKINGBy signing this registration form below, you are committing to placing an order to attend the workshop(s) at the charge(s) shown above. Once complete, please return this registration form by email to [email protected], by fax +44 (0)1959 563123 or by post to Workshops, Russell Publishing, Court Lodge, Hogtrough Hill, Brasted, Kent, TN16 1NU.
Email: [email protected] | Phone: +44 (0)1959 563 311 (ext:3) | Fax: +44 (0)1959 563123
2 SIGNATURE: (MAN DATORY) SIGNATURE: (Mandatory)
Workshop fee includes:• Access to all lectures and practical sessions• Delegate information pack• Buffet lunch plus unlimited refreshments throughout
• 3 x nights local accommodation - breakfast included
• CPD certificate, issued by the Society of Biology
HOW TO BOOK:
a drug target review workshop
CELL-BASED ASSAYSFOR SCREENING Hamburg, Germany
Sponsored by In assocation with
STANDARD RATE ACADEMIC DISCOUNT RATE
With Accommodation - £1,800 +VATWith Accommodation - £1,500 +VAT
Without Accommodation - £1,500 +VATWithout Accommodation - £1,200 +VAT
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