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Life Science Reagents for Northern and Southern Blottingfrom Sigma-Aldrich®

We Ripple Through Everything You Do!• Extensive reagent offering to support your Northern and Southern Blotting based research needs.• Market leading manufacturer of biological buffers• Quality and availability assured• Market leading Technical Service • Leading Web support tools:

Molecular Biology — sigma-aldrich.com/genomicsVirtual Stockroom — sigma-aldrich.com/virtualstockroom

Proteomics

ProteinDetectionWestern Blots

& ELISAs

Cell-BasedAssaysCell

Signaling

RecombinantProtein Expression

& Purification

ProteinElectrophoresis

PAGE & 2D PAGE

Cloning

Nucleic AcidPreparation

DNA & RNA

Transfection CellCulture

Nucleic AcidAmplificationPCR, QPCR, RT-PCR Stem Cell

Culture

Genotyping

Nucleic AcidBlottingDNA & RNA

(Southern) (Northern)

Nucleic AcidElectrophoresis

DNA & RNA

Nuclear Magnetic Resonance (NMR)

2 sigma-aldrich.com For more information, please contact Sigma-Aldrich Customer Service at 800-325-3010.

Sigma-Aldrich’s complete line of nucleic acid electrophoresis reagents• Wide range of agarose products to cover

your melting point, resolution or pulse field requirements

• Full line of DNA ladders and markers

• Single stranded DNA to prevent non-specific binding

• Full line of running and transfer buffers

To learn more, please visit us at sigma-aldrich.com/genomics.

Life Science Reagents for Northern and Southern Blotting from Sigma-Aldrich®

Cat. No. Description

Acids & Bases

H1758 Hydrochloric acid Molecular Biology Grade, 36.5-38.0%

S8045 Sodium hydroxide SigmaUltra, ≥98%, pellets (anhydrous)

Agarose

A5304 Agarose for electrophoresis

A9539 Agarose for molecular biology

A4718 Agarose High resolution, DNase, RNase, NICKase, none detected

A5093 Agarose Low EEO, for molecular biology

A6013 Agarose Type I, Low EEO

A0169 Agarose Type I-A Low EEO

A6877 Agarose Type II, Medium EEO

A2576 Agarose Type IX-A, Ultra-low Gelling Temperature

A0701 Agarose Type VII-A, Low Gelling Temperature

A7431 Agarose Wide range/Standard 3:1, DNase, RNase, NICKase, none detected

Buffers

H4034 HEPES Biotechnology Performance Certified, ≥99.5% (titration), cell culture tested

H3537 HEPES solution 1 M, cell culture tested, Biotechnology Performance Certified

H3784 HEPES sodium salt Biotechnology Performance Certified, cell culture tested, ≥99.5%

H3662 HEPES sodium salt solution 1M, cell culture tested, Biotechnology Performance Certified

H7140 Hybridization Solution, 2× Concentrate, Buffered with SSC, 200μg per mL DNA in 10× SSC, 2× Denhardts

M3183 MOPS Biotechnology Performance Certified, cell culture tested, ≥99.5%

M9024 MOPS sodium salt Biotechnology Performance Certified, cell culture tested, ≥99.5%

M1442 MOPS solution Biotechnology Performance Certified, cell culture tested, 1 M

N6019 Neutralizing buffer for Southern Blots powder blend

N1532 Neutralizing Solution for Neutral Southern Transfer

N1531 Denaturation Solution for Neutral Southern Transfer

H7033 PerfectHyb™ Plus Hybridization Buffer liquid, 1×

P5493 Phosphate buffered saline 10× Concentrate, BPC, cell culture tested

P5368 Phosphate buffered saline pH 7.4, BPC

P3813 Phosphate buffered saline powder, pH 7.4

3Delivering Science through Business Understanding

Cat. No. Description

P9541 Potassium chloride for molecular biology, ≥99%

P9791 Potassium phosphate monobasic for molecular biology, ≥98%

P1415 Prehybridization Solution, 2× Concentrate, Buffered with SSC

S2889 Sodium acetate anhydrous for molecular biology

S6191 Sodium chloride Biotechnology Performance Certified, ≥99.5% (titration), cell culture tested

S3014 Sodium chloride for molecular biology, ≥98%

S6316 Sodium chloride solution 5 M, Biotechnology Performance Certified, cell culture tested

C8532 Sodium citrate tribasic dihydrate Molecular Biology, ~ 99% capillary GC

S3264 Sodium phosphate dibasic for molecular biology, ≥98.5%

S6566 Sodium phosphate monobasic Biotechnology Performance Certified, cell culture tested

S7778 Sodium phosphate tribasic dodecahydrate SigmaUltra, ≥98.0%

S6639 SSC Buffer 20× Concentrate

S2015 SSPE Buffer 20× Concentrate

T9650 Tris Acetate-EDTA buffer 10× Concentrate, DNase and RNase, none detected

T4415 Tris-Borate-EDTA buffer 10× Concentrate

T6066 Trizma® base Biotechnology Performance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9%

T5941 Trizma hydrochloride for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0%

Detergents

P5413 Poly(ethylene glycol) for molecular biology, average mol wt. 8,000

L4390 Sodium dodecyl sulfate for molecular biology, ≥98.5%

General Reagents

M7154 2-Mercaptoethanol for electrophoresis

M3148 2-Mercaptoethanol for molecular biology, ≥98%

B4287 Albumin from bovine serum for molecular biology, powder

B2518 Albumin, Acetylated from bovine serum lyophilized powder, for molecular biology

R7522 All-in-One™ Random-Primed Labeling Mixes −dCTP

A0410 Aminoallyl-dUTP sodium salt ≥85%, lyophilized powder

A5660 Aminoallyl-UTP sodium salt ~80%, lyophilized powder

C0712 CDP-Star® Chemiluminescent Substrate 0.25 mM in H2O

C3032 Cesium chloride for molecular biology, ≥99%

Cat. No. Description

496189 Chloroform biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

D2532 Denhardt’s Solution 50× Concentrate for molecular biology, liquid, A 1% solution of BSA, Ficoll® and PVP

D9905 Denhardt’s Solution Lyophilized Powder for molecular biology

D8906 Dextran sulfate sodium salt from Leuconostoc spp. for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer

494429 Dimethyl sulfoxide Biotech grade solvent, 99.8%

E5134 EDTA disodium salt dihydrate for molecular biology, ≥99%

E7023 Ethanol 200 proof (absolute), for molecular biology

E1510 Ethidium bromide solution 10 mg/mL, for molecular biology, aqueous solution

F8775 Formaldehyde solution for molecular biology, 36.5-38% in H2O

F5786 Formamide SigmaUltra, ≥99%

G5516 Glycerol for molecular biology, ≥99%

H3400 Heparin sodium salt from porcine intestinal mucosa average mol wt. ~3,000

M1028 Magnesium chloride solution for molecular biology, 1.00 M±0.01 M

P1037 Phenol for molecular biology

P5288 Polyvinylpyrrolidone for molecular biology, nucleic acid hybridization tested, mol wt. 360,000

T9281 TEMED for electrophoresis, ~99%

T3411 Tetramethylammonium chloride solution for molecular biology

W3513 Water Biotechnology Performance Certified

W4502 Water Molecular Biology Reagent

Membranes / Blotting Paper

N6156 Biobond-Plus™ Nylon transfer membrane disc size 132 mm

N5281 Biobond-Plus Nylon transfer membrane sheet size 11.9 cm × 7.8 cm

N1281 BioBond™ Nylon transfer membrane roll size 20 cm × 3.5 m

P8046 Blotting paper, pure cellulose Extra Thick, size 10 cm × 15 cm

N7892 Nitrocellulose membranes for blotting pore size 0.2 μm, size 15 cm × 15 cm

N8017 Nitrocellulose membranes for blotting pore size 0.2 μm, size 20 cm × 20 cm

N8267 Nitrocellulose membranes for blotting pore size 0.45 μm, size 15 cm × 15 cm

N1389 Nylon membranes, charge modified for hybridization pore size 0.45 μm, size 20 cm × 20 cm

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Order/Customer Service (800) 325-3010 • Fax (800) 325-5052

Technical Service (800) 325-5832 • sigma-aldrich.com/techservice

Development/Bulk Manufacturing Inquiries (800) 244-1173

©2008 Sigma-Aldrich Co. All rights reserved. SIGMA, , SAFC, , SIGMA-ALDRICH, ALDRICH, , FLUKA, , and SUPELCO, are trademarks belonging to Sigma-Aldrich Co. and its affiliate Sigma-Aldrich Biotechnology, L.P. Sigma brand products are sold through Sigma-Aldrich, Inc. Sigma-Aldrich, Inc. warrants that its products conform to the information contained in this and other Sigma-Aldrich publications. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Please see reverse side of the invoice or packing slip. Trizma, Sigma and Sigma-Aldrich are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. PerfectHyb, All-in-One, BioBond and Biobond-Plus are trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. CDP-Star is a registered trademark of Tropix, Inc. Sigma and Sigma-Aldrich are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Ficoll is a registered trademark of GE Healthcare.

Nucleic Acid Electrophoresis and Blotting

Agarose and AcrylamideUsed in routine electrophoresis of nucleotides for DNA identification, Southerns, Northerns, or DNA recovery. Sigma offers a wide range of agarose products depending on the desired result, such as low melting point, high resolution, or pulse-field gels. Acrylamide is used to perform Polyacrylamide Gel Electrophoresis (PAGE) gels for protein or high-resolution nucleic acid electrophoresis.

Precast GelsTo speed up your electrophoresis workflow Sigma offers a variety of precast gels for nucleic acid electrophoresis. The precast gels have a variety of concentrations and wells available to run gels specific to the needs of each experiment.

Denhardt's SolutionMixture of blocking agents used in hybridization protocols such as Northerns and Southerns to prevent non-specific binding of probes.

DNA Ladders and MarkersSigma has an assortment of nucleic acid markers for use in agarose or PAGE electrophoresis. The range and number of bands in each ladder varies depending on the size of nucleotide for identification. Ladders are also optimized for use at specific gel concentrations.

Dyes and Loading BuffersDyes, such as Xylene Cyanol and Bromophenol Blue are added to nucleic acid samples to track the migration of samples as agarose or PAGE electrophoresis occurs. Sigma also provides Gel Loading Buffers that contain components such as sucrose to facilitate sample loading.

Nucleic Acid Gel StainsEthidium bromide is an intercalation agent used to identify nucleotides after conducting electrophoresis. When placed inside a UV light box ethidium bromide fluoresces enabling identification of samples.

Running BuffersBuffers such as Tris-Borate-EDTA (TBE) are most commonly used for PAGE gels while Tris-Acetate-EDTA (TAE) is used for agarose electrophoresis. Sigma has a broad portfolio of Running Buffers depending on the specific running conditions. Bionic Buffer enables gels to be run at higher voltages resulting in runtimes that are 2–3 times faster than TBE or TAE.

ssDNAUsed to prevent non-specific binding of probes to membranes when Northern or Southern blots are performed.

EquipmentSigma-Aldrich has an assortment of equipment used in electrophoresis and blotting, of which gel boxes, power supplies, and pipette tips are available. Barrier pipette tips are used to prevent sample contamination.

Learn more at sigma-aldrich.com/genomics.