Leibniz Institute of Plant Genetics

and

Crop Plant Research (IPK)ECPGR

Working Group on Forages

Poel, 29.4.2010

Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

Gatersleben

Leibniz Society (http://www.wgl.de)

Gottfried Wilhelm Leibniz 1646-1716 "theoria cum praxi"

86 non-university research institutes and service facilities

Research activities of Leibniz Institutes range from pure

fundamental research to the investigation of highly applied issues

Institutes are assessed at regular intervals by external experts

5 Sections- Humanities and Education

- Economics, Social Sciences,

Regional Infrastructure

- Life Sciences

- Mathematics, Natural Science, Engineering

- Environmental Sciences

Budget: 860 Million €

Grants: 244 Million €

Staff: 13.930

Scientists: 6.347

PhD Students: 1.6048.5.2009

Genome DiversityProf Andreas Graner

Dr Nils Stein

Resources

Genetics and

ReproductionDr Andreas Börner

1) Management

Governing BoardChair:

MinDirig Dr Joachim Welz

Vice Chair:

RD Peter Hassenbach

Managing OfficeRoland Schnee

Characterisation and

DocumentationProf Andreas Graner

Management and

EvaluationDr Andreas Börner

Taxonomy and

EvolutionDr Frank Blattner

Dept. of Genebank

Head: Prof Andreas Graner

Dept. of Administration

and Central ServicesHead: Bernd Eise

Dept. of Cytogenetics and

Genome AnalysisHead: Prof Ingo Schubert

Dept. of Molecular

Genetics

Head: Prof Thomas Altmann

Dept. of Physiology and

Cell Biology

Head: Prof Nicolaus von Wirén

Karyotype EvolutionProf Ingo Schubert

Seed DevelopmentDr Winfriede Weschke

Plant Genome Resources Centre (PGRC) Coordinator: Dr Patrick Schweizer

Bioinformatics Coordinator: Prof Falk Schreiber

Genebank

DocumentationDr Helmut Knüpffer

Satellite

Collections

North Dr Klaus Dehmer

In vitro Storage and

CryopreservationDr Joachim Keller

Taxonomy of Plant

Genetic ResourcesDr Frank Blattner

(prov.)

Experimental

TaxonomyDr Frank Blattner

Groups

Transcriptome AnalysisDr Patrick Schweizer

Chromosome Structure

and FunctionDr Andreas Houben

Apomixis Dr Timothy F. Sharbel

EpigeneticsDr Michael F Mette

In vitro DifferentiationProf Anna M Wobus

Gene and Genome

MappingDr Marion Röder

Bioinformatics and

Information TechnologyDr Uwe Scholz

Programmes

CytogeneticsProf Ingo Schubert

Plant BioinformaticsProf Falk Schreiber

Gene RegulationDr Helmut Bäumlein

PhytoantibodiesDr Udo Conrad

Plant Reproductive BiologyDr Jochen Kumlehn

Applied BiochemistryDr Hans-Peter Mock

Structural Cell BiologyDr Michael Melzer

Yeast GeneticsProf Gotthard Kunze

PersonnelJuliane Becker

FinancesMartina Liewald

Technology Transfer and

Legal MattersDr. Tankred Schuhmann

Buildings and EquipmentHerwig Bierstedt

Materials, Management and

General ServicesUrsula Deppner

Experimental Fields and

NurseriesPeter Schreiber

Genome AnalysisDr Patrick Schweizer

Genome PlasticityDr Renate Schmidt

Board of DirectorsProf Andreas Graner 1)

Managing Director

Sybille-Andrea Lorenz 1)

Administrative Director

Prof Ingo Schubert

Prof Thomas Altmann

Prof Nicolaus von Wirèn

Scientific Advisory BoardChair:

Prof Christian Jung

Personnel CouncilChair: Thomas Kruse

Genebank Advisory BoardChair:

Dr Christiane Gebhardt

Molecular Plant NutritionProf Nicolaus von Wirén

Data Inspection(Junior Research Group)

Dr Marc Strickert

Hybrid Wheat(BMBF-funded Research

Group)

Dr Mario Gils

Plant Architecture(BMBF-funded

Research Group)

Dr Thorsten SchnurbuschExpression MappingDr Lothar Altschmied

HeterosisProf Thomas Altmann

Systems Biology(BMBF-funded Research Group)

Dr Björn H. Junker

Scientists

131

PhD Students

58

Technical and

administrative employees

285

Genome DiversityProf Andreas Graner

Dr Nils Stein

Resources

Genetics and

ReproductionDr Andreas Börner

1) Management

Governing BoardChair:

MinDirig Dr Joachim Welz

Vice Chair:

RD Peter Hassenbach

Managing OfficeRoland Schnee

Characterisation and

DocumentationProf Andreas Graner

Management and

EvaluationDr Andreas Börner

Taxonomy and

EvolutionDr Frank Blattner

Dept. of Genebank

Head: Prof Andreas Graner

Dept. of Administration

and Central ServicesHead: Bernd Eise

Dept. of Cytogenetics and

Genome AnalysisHead: Prof Ingo Schubert

Dept. of Molecular

Genetics

Head: Prof Thomas Altmann

Dept. of Physiology and

Cell Biology

Head: Prof Nicolaus von Wirén

Karyotype EvolutionProf Ingo Schubert

Seed DevelopmentDr Winfriede Weschke

Plant Genome Resources Centre (PGRC) Coordinator: Dr Patrick Schweizer

Bioinformatics Coordinator: Prof Falk Schreiber

Genebank

DocumentationDr Helmut Knüpffer

Satellite

Collections

North Dr Klaus Dehmer

In vitro Storage and

CryopreservationDr Joachim Keller

Taxonomy of Plant

Genetic ResourcesDr Frank Blattner

(prov.)

Experimental

TaxonomyDr Frank Blattner

Groups

Transcriptome AnalysisDr Patrick Schweizer

Chromosome Structure

and FunctionDr Andreas Houben

Apomixis Dr Timothy F. Sharbel

EpigeneticsDr Michael F Mette

In vitro DifferentiationProf Anna M Wobus

Gene and Genome

MappingDr Marion Röder

Bioinformatics and

Information TechnologyDr Uwe Scholz

Programmes

CytogeneticsProf Ingo Schubert

Plant BioinformaticsProf Falk Schreiber

Gene RegulationDr Helmut Bäumlein

PhytoantibodiesDr Udo Conrad

Plant Reproductive BiologyDr Jochen Kumlehn

Applied BiochemistryDr Hans-Peter Mock

Structural Cell BiologyDr Michael Melzer

Yeast GeneticsProf Gotthard Kunze

PersonnelJuliane Becker

FinancesMartina Liewald

Technology Transfer and

Legal MattersDr. Tankred Schuhmann

Buildings and EquipmentHerwig Bierstedt

Materials, Management and

General ServicesUrsula Deppner

Experimental Fields and

NurseriesPeter Schreiber

Genome AnalysisDr Patrick Schweizer

Genome PlasticityDr Renate Schmidt

Board of DirectorsProf Andreas Graner 1)

Managing Director

Sybille-Andrea Lorenz 1)

Administrative Director

Prof Ingo Schubert

Prof Thomas Altmann

Prof Nicolaus von Wirèn

Scientific Advisory BoardChair:

Prof Christian Jung

Personnel CouncilChair: Thomas Kruse

Genebank Advisory BoardChair:

Dr Christiane Gebhardt

Molecular Plant NutritionProf Nicolaus von Wirén

Data Inspection(Junior Research Group)

Dr Svetlana Friedel

Hybrid Wheat(BMBF-funded )

Dr Mario Gils

Plant Architecture(BMBF-funded

Research Group)

Dr Thorsten SchnurbuschExpression MappingDr Lothar Altschmied

HeterosisProf Thomas Altmann

Systems Biology(BMBF-funded Research Group)

Dr Björn H. Junker

Research Mission

IPK is a research centre that deals with questions of

modern biology, mainly by studying cultivated plant

species.

Its research aims at the generation of novel knowledge, the

discovery of basic principles of plant performance and the

development of enabling technologies.

These are considered to be of paramount importance for

the targeted utilization of biological diversity for the

sustainable production of food, feed and renewable

resources.

1961/63 2005/2007 2050

Population growth and agricultural

production

Data from Bruinsma, 2009

FAO Expert Meeting,

Rome

0

1000

2000

3000

4000

5000

6000

7000

8000

9000

10000

World population

(mil

l. p

eo

ple

)

38%

Cereals production

(mil

l. t

on

s)

0

1000

2000

3000

49%

Meat production

(mil

l. t

on

s)

0

100

200

300

400

500

600

700

800

900

1000

85%

Renewable energies

Quelle, BMU 2010

Quelle: OECD/IEA World Energy Outlook 2004

Zebisch et al. 2005 Klimawandel in Deutschland

Global Change

Increase in arable land by 2050

120 mill ha (7%)

Data from Bruinsma, 2009

FAO Expert Meeting,

Rome

Arable land per caput (ha)

Bruinsma, 2009

FAO Expert Meeting, Rome

Competing demands

- Population increase

- Urbanization

- Industrial use

- Biofuel production

- Land conservation

- Climate change

Interdisciplinary Approaches by

Integration of

Cell Biology

Biochemistry

Physiology

Molecular Genetics

Cytogenetics

Taxonomy

Bioinformatics

Wx

ABG704 ABG312Ris17aGBR399GBM1126MWG2018GBR0961GBS0018MWG042b GBR297MWG555aGBR282G-alphaGBR605GBR507 GBS028Bmag021GBR161GBM1060GBR0923GBR046GBR104GBR470GBR464GBS0035MWG089GBR226

GBR538 GBR019

GBR152GBS0154MWG2291ABG380GBR219GBR405GBM1116GBS0356MWG836GBR602GBR036GBM1033

GBR074GBR637GBS0198GBR013MWG2072GBR211GBS0365GBR202GBS0268GBS0413GBR395

GBR222GBM1030GBR0597 GBR088GBM1115GBR0943Bmac273 MWG2031

Ris15GBR159bGBS0378ABG476GBS0360 GBR1143MWG626GBS0340

GBR100GBS0317GBR683 GBR0837GBR1043aGBS0250GBR0632GBR595GBR283GBS0132GBM1058GBR256GBS0405 GBR102GBM1164GBS0040GBM1359GBR603MWG825GBM1006GBR323bGBR633GBR086dABC310bGBR313

GBM1102GBR388GBR429GBR539Ris44GBR159aGBR192GBR646bABC253ABG461aWG380aGBR251GBR646cGBR1018GBR660GBS0084GBR0874GBM1017GBS0291GBM1065

GBR1086GBR353aGBR0905

MWG2062

0,04,55,25,46,06,17,98,0

8,29,710,110,912,212,313,014,215,218,721,5

22,022,826,528,129,430,330,431,331,431,732,833,8

42,242,745,652,554,659,660,861,664,265,168,068,668,868,969,970,370,570,871,273,274,074,374,574,875,075,277,677,978,578,678,879,479,680,280,8

81,483,183,383,984,385,285,488,990,691,892,694,3

97,998,099,9

101,0101,3102,6103,6104,3104,7106,5111,2

113,1115,5120,2123,3126,0

126,5127,9128,0128,8131,8133,7134,0

135,6137,8142,5143,4144,9145,1146,8

Dor4b

Arabidopsis

thaliana

Model Plants

Tabacco

Nicotiana sp.

St. John´s WortHypericum perforatum

Rock CressBoechera holboellii

Crop Plants

Barley

Wheat

Legumes

Maize

Rapeseed

Potato

Forage Grasses

Research Areas

Conservation and

Utilization of Crop Plant

Diversity

Dynamics of Plant

GenomesIntegrative Biology

of Plant Performance

Improvement of seed, in

vitro and cryo conservation.

Genomics approaches to

understand and valorize

genetic diversity.

Understanding genome

diversity on a structural

and functional level.

Use of high throuput technologies

to study plant performance at a

cellular and organismic level.

• Completion of the collection

• Optimization of conservation procedures/seed longevity

• Development of genebank related information systems

Optimization of the conservation management

Conservation and Utilization of Crop

Plant Diversity

• Taxonomic research

• Adaptation, speciation and domestication

Taxonomy and evolutionary biology

• Development of resources (populations, markers, maps)

• Evaluation/phenotyping

• Trait mapping, gene isolation

• Analysis of allelic diversity (allele mining)

Development of strategies for an improved

utilization of genetic resources

22.05LOD

3.0

MWG851B

MWG851C

ABG314B

ABG463

CDO484

ABG390

ABG391

ABC482

ABC155

ABG495A

MWG877

WG908

iEst9

CDO504

MWG514B

WG364

mSrh

CDO57B

ABC302

ABC324

WG889

WG530

WG541

ksuA3A

Ubi2

CDO749

ABG395

ABG708

ABG705

ABG316B

MWG920-1A

ABC483

MWG502

Mad2

0.022.05LOD

3.0

MWG851B

MWG851C

ABG314B

ABG463

CDO484

ABG390

ABG391

ABC482

ABC155

ABG495A

MWG877

WG908

iEst9

CDO504

MWG514B

WG364

mSrh

CDO57B

ABC302

ABC324

WG889

WG530

WG541

ksuA3A

Ubi2

CDO749

ABG395

ABG708

ABG705

ABG316B

MWG920-1A

ABC483

MWG502

Mad2

0.0

• Seed development and -physiology in cereals, legumes and

Brassicaceae

• Formation and evolution of plant centromeres

• Evolutionary and molecular analysis of sexual und apomictic

reproduction

• Genetic principles and molecular mechanisms of heterosis

• Genetics of non-host resistance in cereals to fungal pathogens

• Effect of plant nutrition on phytohormones and morphologic

differentiation

• Physical mapping and whole genome sequencing in barley

Flagship projects

MetabolomicsMetabolites

GenomicsDNA

ProteomicsProteins

TranscriptomicsRNA

Infrastructure

0

10

20

30

40

50

60

70

80

90

104

100 113 125 138 150 163 175 188 200 213 225 238 250 263 275 288 300 313 325 338 350 363 375 388 400

Glc-6-P AV: 19.04-19.07 min (3) SB: 4.73-5.14 (25) NL: 2.53E4 T: {0,0} - c ESI corona sid=50.00 det=1435.00 Full ms [ 100.00-400.00]

173.0

212.1

112.9

233.0

155.0

215.1191.1

145.0218.2

259.1251.2

181.0 343.3

161.1

380.1266.5

205.2352.5132.9

169.2 230.3 374.3300.3277.8 309.0 328.6 388.5290.1119.9 248.2 334.8273.5 326.0 366.7

%

amu

Phenotypic analysis

PLANT BIO-

INFORMATICS

Falk Schreiber

DATA INSPECTION

Svetlana Fiedler

SYSTEMS

BIOLOGY

Björn H. Junker

GENEBANK

DOCUMENTATION

Helmut Knüpffer

BIOINFOR-

MATICS &

INFORMATION

TECHNOLOGY

Uwe Scholz

Bioinformatics@IPKCoordinator: Falk Schreiber

Analysis of natural diversity Transgenic approaches

wild typeA1A2

22.05LOD

3.0MWG851B

MWG851C

ABG314B

ABG463

CDO484

ABG390

ABG391

ABC482

ABC155

ABG495A

MWG877

WG908

iEst9

CDO504

MWG514B

WG364

mSrh

CDO57B

ABC302

ABC324

WG889

WG530

WG541

ksuA3A

Ubi2

CDO749

ABG395

ABG708

ABG705

ABG316B

MWG920-1A

ABC483

MWG502

Mad2

0.022.05LOD

3.0MWG851B

MWG851C

ABG314B

ABG463

CDO484

ABG390

ABG391

ABC482

ABC155

ABG495A

MWG877

WG908

iEst9

CDO504

MWG514B

WG364

mSrh

CDO57B

ABC302

ABC324

WG889

WG530

WG541

ksuA3A

Ubi2

CDO749

ABG395

ABG708

ABG705

ABG316B

MWG920-1A

ABC483

MWG502

Mad2

0.0

The Federal ex situ Genebank

Conservation , Service, Research

- Collection, conservation and documentation of

plant genetic resources

- Research on cultivated plants and their wild

relatives and related species

- Distribution of plant material for research, breeding

and education

Mission

The Federal ex-situ Genebank

National Strategy for Agrobiodiversity

- secure and enlarge infrastructure for PGR conservation

- develop approaches and strategies for utilization of PGR

- enhancement of international collaboration

Integration at the EU level

- ECPGR

- AEGIS

- Framework 6,7

The Federal ex-situ Genebank

Total Collection

146.966 Accessions

2.649 Species

779 Genera

Reference Collections

391.000 Herbarium sheets

90.000 Seeds & Fruits

49.000 Spike-Samples

Cereals & Grasses 64.104

Legumes 28.250

Vegetables 18.538

Oil-/ Fiber plants 9.737

Medicinal-/ Spice plants 5.951

Mutants 2.684

Forage plants 12.406

Potatoes 5.874

Total 146.966

Collections Accessions

22.350

Safety Duplicates

Ex-situ Conservation

• Cold room storagee.g. cereals, legumes

• Field conservatione.g. fruit trees, Allium

• In-vitro culturee.g. potatoes, Allium

• Cryo conservatione.g. potato

2008/9: 22.799 Samples

Durchschnittliche Bearbeitungsdauer 2008/2009: 8,5 Tage ab Eingang SMTA

Forschungs-

Einrichtungen

(276)

10.092

Genbanken

(6) 48

Botanische Gärten

(10) 232

Züchter

(80)

3.637

IPK

(48)

805

NGOs

(49)

2.500

Privatpersonen

(270) 3.114

Behörden

(12)

1.042

Sonstige

(30)

1.329

Forschungs-

Einrichtungen

(276)

10.092

Genbanken

(6) 48

Botanische Gärten

(10) 232

Züchter

(80)

3.637

IPK

(48)

805

NGOs

(49)

2.500

Privatpersonen

(270) 3.114

Behörden

(12)

1.042

Sonstige

(30)

1.329

Distribution of Seeds and Plant Material

Certified Quality Management

documentation, transparency, know how

- Primary cultivation

- Characterization

- Reference vouchers

- Harvest/drying

- Germination test

- Storage

- Distribution

- Multiplication

- Research

- Human Resources

Conservation of

Biodiversity

Along the biodiversity value chain

Research into

Biodiversity

Utilization of

Biodiversity