Lecture 21

HPLC-2Instrumentation

ColumnInjector Detector

Chromatograph

Data Processing

Percentage of solvents having the same eluent strength

B - acetonitrile CH3CN

constant

constant constant 162

2

2

2

W

t

W

t

W

tN

r

rr

Width W is proportional to retention time tr

‘general chromatography problem’

Solution of GNP: gradient

Pump

4.5 mm2 HPLC columns of different length

Injector

Detectors

UV cell

Derivatization in HPLC

Evaporative Light- Scattering Detector

HOOH

HO1

HOOH2

HOOH3

CH3OH

CH3CH2OH

OH

4

5

6

OH7

OHHO

O OH

13 14

Electrochemical detection of alcohols in water

8 2-butanol 9 2-Methyl-1-propanol10 1-butanol 11 3-methyl-1-butanol12 1-pentanol

Chemistry: Oxidation of alcohols @Au electrode

MS Detector