Leclercq (Overview of standard methods development of ISO and CEN)

Overview of standard methods development of ISO and CEN

Alexandre LECLERCQ

Convenor of CEN TC275/WG6 Food Microbiology

IAFP, Berlin, 20090/50

CRITERIA / STANDARD (Codex alimentarius, CAC/GL 21 of 1997)

• Micro-organisms of interest - interest for the product ;• Sampling plan;• Usable detection and/or quantification methods• Satisfactory microbiological limits for the food at the

critical point of the food chain ;• The number of analytical units allowing a conformity

compared to these limits ;

• The food to which it applies• Stages of the food chain to which it applies ;• All measures to be taken when it is not satisfied.


FoodFood

Human/animalHuman/animal

WaterWater EnvironnementEnvironnement

Target: Which Microorganisms ?Prions Prions

??

VirusVirus

Toxins Toxins (Algae, Fungi, bact.)(Algae, Fungi, bact.)

ProtozoaProtozoa

ParasitesParasites

Bacteria/Bacteria/

SporesSpores

FungiFungiInsectsInsects

MetabolitesMetabolites(Fungi, bact.)(Fungi, bact.)

Toxicology ?

GMO ?GMO ?

BacteriophagesBacteriophages


Example: Bacteria• Pathogen

• Bacteria resistant to antibiotics• Opportunistic

• Deterioration flora

• Bioindicator flora– Sanitary– Technology

• Probiotics

• Floral with technological interest / Starter

• Flora of Ali-terrorism

1 microorganism

1 Population,

1 ecology


The target becomes more and more complex!Example of the producing E coli of Shiga-like toxins

• Serotype detection– Interesting public health: as EHEC– Interesting veterinary sector

• Detection of virulence genes– VT/eaeA/HlyA

• Test in Vivo / cellular culture

• Immunological detection of verotoxins!


Viable but not cultivable

Culture Enumeration

Survival

Colony viability

Time

Log CFU

�� Molecular biology / Cytometry / Microscopy


PROCESS: ASSAY

Sample collection

Manutention

Preparation

ANALYSES

Results

Report

Personnal

Premises

Working conditions

Equipment

Methods ??

Reagents

Calibration

Quality Control

Recording Audit

Management

Quality System

Validation

Measurement uncertainty


Standardized methodsStandardized methods

• Reference text

• Elaborated by qualified body, with consensus among all the interested parties

• Continuous, repetitive and voluntary application

« Speak the same language »

Distinction between full standard and Technical specification


Standardize or Harmonize:Standardize or Harmonize:Why ?Why ?

• Avoid disparate methods of microbiological analysis

• Facilitate exchange of food products

• Improve reliability of microbiological analysis

• Relevant to general development of quality assurance


International StandardizationInternational Standardization(1947)(1947)

• ISO (50 Standardization bodies)

• ISO standard: optional integration in national collection, revision each 5 years

• TC34: Agricultural food products

� SubCommittee 9: Microbiology: horizontal methods

� Chairman: B. LOMBARD,

� Secretariat: C. DE LAMOTTE

• Vienna agreement: ISO standards �� CEN standards

Scope: Standardization in the field of human and animal foodstufScope: Standardization in the field of human and animal foodstuffs, fs, covering the food chaincovering the food chainfrom primary production to consumption, a well as animal and vegfrom primary production to consumption, a well as animal and vegetable propagation etable propagation materials, in particualr, but not limited to, termi nology, samplmaterials, in particualr, but not limited to, termi nology, sampling, methods of test and ing, methods of test and analysis, product specifications, food and feed safety and qualianalysis, product specifications, food and feed safety and quality of management and ty of management and requirements for packaging, storage and transportation.requirements for packaging, storage and transportation.excluded: products covered by ISO/TC54 Essential oils and ISO/TCexcluded: products covered by ISO/TC54 Essential oils and ISO/TC93 (Starch)93 (Starch)

Mission: « Standard horizontalisation »IAFP, Berlin, 200910/50

FunctioningISO CASCO/BT

ISO TC34

SC9

WG4Proficiency

testing

WG5Culture

media

WG6

Parasites

Project

groupProbiotics

WG7Microbiological examinations

WG3

Method

Validation

WG2

Statistics

WG1Meat and

meat products


European Standardization (1985)European Standardization (1985)

• CEN (Bruxelles, 19 Standardization Bodies),

• TC275: Food Analysis / WG6 Microbial Contaminant• Chairman: A. LECLERCQ, • Secretariat: C. DE LAMOTTE

EN Standard: Obligatory integration in national collection

• Pre-established recognition of standards ( by ISO)

Scope: Standardization of horizontal microbiological Scope: Standardization of horizontal microbiological methods for all food and animal feeding stuff analysis methods for all food and animal feeding stuff analysis and for any other sample that can be the source of and for any other sample that can be the source of microbiological contamination of food productsmicrobiological contamination of food products


FunctioningCEN BT

TC 275

WG6ISO TC34/SC9

TAG3

PCR

TAG4

Virus

TAG5Primary

production

TAG6

Sampling techniques

TAG1

Media

TAG2

Validation

Ad’hoc

Group

STEC

TC 302MILK

TC 327Probiotics

EU PROJECT

TAG7

Parasites

Ad’(Hoc group

C. sakazakii

Ad’Hoc group

L. monocytogenes

Ad’Hoc group

Histamine, B. cereus toxins, Staph. toxins


Alternative method # Full standard?

For a given scopegiven scope, it shall estimate the same same analyteanalyte than the one measured by the corresponding reference method,

and shall meet the following requirements:–– rapidrapid analysis and/or response

–– easyeasy carrying and/or automation

– analytical performancesperformances (accuracy, sensitivity..)


Standard and Outbreak(ISO TC34/SC9 and CENTC275/WG6)

Possible Introduction of the following text as a note in the scope of the standards on pathogen detection

“For epidemiological purposes or during outbreak investigations, other methods may need to be used in addition to this standard”.

A standard could not cover all matrices and all target microorganisms!


Microbiology of food and animal feeding stuffs

““ Microbiology of the food chainMicrobiology of the food chain””

According to the DG SANCO agreement, the scope of WG6 is enlargeAccording to the DG SANCO agreement, the scope of WG6 is enlarged to include d to include bottled water and water used in food processing only when standabottled water and water used in food processing only when standardized water rdized water

microbiology methods do not exist.microbiology methods do not exist.IAFP, Berlin, 200916/50

General requirements and guidance for General requirements and guidance for microbiological examinationsmicrobiological examinations

Amemdment of EN ISO 7218 published in 2007Amemdment of EN ISO 7218 published in 2007(ISO TC34/SC9/WG7, A. Leclercq)(ISO TC34/SC9/WG7, A. Leclercq)

•General requirements and guidance/options intended for three main uses:

•implementation of ISO/TC 34/SC 9 or ISO/TC 34/SC 5 standards for detection or enumeration of microorganisms, named hereafter “specific standards”;

•good laboratory practice for food microbiological laboratories (the purpose is not to detail them in this International Standard, manuals are available for that purpose);

•guidance for accreditation of food microbiological laboratories (this International Standard describes the technical requirements according to Annex B of ISO/IEC 17025:2005 for the accreditation of a microbiological laboratory by national organizations).

•covers examination for bacteria, yeasts and mouldsand can be used if supplemented with specific guidance for prions, parasites and viruses. It does not cover the examination for toxins or other metabolites (e.g. amines) from microorganisms.

• applies to the microbiology of food, animal feeding stuffs, the food production environment and the primary production environment .

•The purpose of this International Standard is to help to ensure the validity of food microbiology examinations, to assist in ensuring that the general techniques used for conducting these examinations are the same in all laboratories, to help achieve homogeneous results in different laboratories, and to contribute towards the safety of the laboratory personnel by preventing risks of infection.


Microorganisms


Salmonella: Revision of EN ISO 6579(ISO TC34/SC9)

• Part 1: Detection and AmdA1 (primary production samples)• deal with the detection of S. Typhi and S. Paratyphi in a normative annex

considering the use of the SC enrichment broth• launch a trial comparing enrichment in the BAM/USP formulation of tetrathionate

broth and in MKTTn.• collect data comparing the use of RVS and MSRV for food analysis• XLD should be retained as the mandatory isolation medium• usefulness of some biochemical tests

• Part 2: Enumeration• a new MPN method, able to analyze simultaneously a lot of samples at a lower

cost. This technique is based on the ability of the MSRV medium to select and enriched motile Salmonellathat may be further isolated on chromogenic medium

• Part 3: Serotyping• Based on Kauffman white’s scheme


Campylobacter(CEN TC275/WG6)

Revision of EN ISO 10272-1:2006 (detection Campylobacter) and of ISO/TS 10272-2:2006 (enumeration Campylobacter – Colony-count technique).

To improve the enrichment and confirmation steps and microaerobic incubation conditions


Listeria spp. detection and enumeration(CEN TC275/WG6/ad’hoc group, R. Beumer)

Revision of EN ISO 11290

L. monocytogenes� Listeria spp. and L. monocytogenes

Simplification of identification


Revision EN ISO 11290: Identification Scheme

Presumptive L.monocytogenes

Other presumptive Listeria species

ALOA or other chromogenic medium

Gram stain (optional)

Haemolysis

CAMP (if necessary)

Rhamnose

Xylose

Gram stain

Haemolysis

CAMP (if necessary)

VP test

Further tests if speciation required (rhamnose, xylose, mannitol)

Second medium if not chromogenic

Gram stain

Haemolysis

CAMP (if necessary)

RhamnoseXylose

Gram stain

Haemolysis

CAMP (if necessary)

VP test

Further tests if speciation required (rhamnose, xylose, mannitol)


VTEC non-O157(CEN TC275/WG6/ad’hoc group, S. Morabito (CRL))

o Project technical specification

o CEN ISO/WD TS 13136Microbiology of food and animal feeding stuffs -- Horizontal method for the detection of Shiga toxin-producing Escherichia coli (STEC) belonging to O157, O111, O26, O103 and O145 serogroups -- Qualitative Real-time polymerase chain reaction (PCR)-based method

o First detection of stx,eae genes and after RT-PCR for serotypes followed by isolation of colonies

o Problems of patents: RT-PCR for serogroup detection

o A new need: An European standard for stx characterization by PCR or RT-PCR


Bacillus cereus /Bacillus anthracis

Revision of EN ISO 7932:2004 Microbiology of food and animal feeding stuffs -Horizontal method for the enumeration of presumptive Bacillus cereus - Colony-count technique at 30 °C

�Investigation of chromogenic media

�Investigation of the need of a Bacillus anthracis standard


Enterobacter sakazakii(CEN TC275/WG6/ad’hoc Group, H. Joosten & C. Iversen)

• Draft: Horizontal method for the detection of Cronobacterspp. (Enterobacter sakazakii)

� Relative poor performance with environmental samples

� Recovery some sensitive strains from some milk powders

• Enrichment broth is a Cronobacter screening broth:• Need to investigate sub cultures of negative broths and the

possibility of recoloration of the broth


Brochothrix thermosphacta(ISO TC34/SC9/WG2 (meat and meat products), E. De Boer)

Possible Revision of ISO 13722

In parallel with NMKL


Bioindicator - Coliforms: Evolution of methods

Gaz and acid production d’acides from lactose (old definition)

•Escherichia coli

•Klebsiella

•Enterobacter

•Citrobacter

•Escherichia coli

•Klebsiella

•Enterobacter

•Citrobacter

•Yersinia

•Serratia

•Hafnia

•Pantoea

•Kluyvera

•Escherichia coli•Klebsiella•Enterobacter•Citrobacter•Yersinia•Serratia•Hafnia•Pantoea•Kluyvera•Cedeca•Ewingella•Moellerella•Leclercia•Canella•Yokenella

Acid production from lactose (new definition) Enzymatic methods (new

definition)

In green, coliforms present in environment and human faeces

Underlined, coliforms from environment


Enterobacteriaceae: Bioindicator (ISO TC34/SC9, E. de Boer)

• Enterobacteriaceae– Detection and enumeration (ISO 21528:2004)

• Revise part 1 of ISO 21528 by omitting the EE broth enrichment step and to modify the confirmation step(fermentation of glucose has been changed in the the use of O/F medium).

• Establish an amendment to part 2 of ISO 21528 to modify the confirmation step in order to develop the 2 parts at the same time


Virus (CEN TC275/WG6/TAG4, R. Rangdale (CRL))

Draft of a full standard:

Horizontal method for detection of hepatitis A virus and norovirus in food using real-time RT-PCR- Part 1: Method for quantitative determination

Norovirus genogroups I and II

New target matrices: According to regulation EC 178/2002, bottled water and water used in the food industry is a food.


Parasites(ISO TC34/SC9/WG6 and CEN TC275/WG6/TAG7, P. Rossi (CRL))

• Work at ISO level (call of experts): • Cryptosporidium• Giardia

• work at CEN level: Standardize both • the artificial digestion microscopic method for the

detection of Trichinella in muscles and• serological methods for detection of antibodies to

Trichinella.

• Problem of reference material and antigens.


Yeast and moulds(ISO TC34/SC9, A. Leclercq)

•aw ≤ 0.95 et >0.6

•Media DG18

•ISO 21527-2

•aw >0.95

•Media DRBC

•ISO 21527-1


METHODS


TAG 6 "Sampling techniques"(CEN TC275/WG6/TAG6, M. Poumeyrol)

• Revision of EN ISO series : Preparation of test samples, initial suspension and decimal dilutions for microbiological examination

• Agreement with the development of standards about sampling techniques.


General requirements relating to Molecular methods for detection and quantification of microorganisms

(CEN TC275/WG6/TAG3, K. Berghof)

• Standards: General rules for PCR and validation of thermocycler

• New project standards for Real Time - PCR

• EN ISO/DIS 22119Microbiology of food and animal feeding stuffs -- Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens -- General requirements and definitions

• EN ISO/DIS 22118Microbiology of food and animal feeding stuffs -- Polymerase chain reaction (PCR) for the detection of food-borne pathogens -- Performance characteristics of molecular detection methods

� The performance characteristics of molecular methods shall be established according to the procedures of EN ISO 16140 if applicable

• DNA Biochips?

• New part of Scope: Primary production stage


CEN ISO/TS PCR detection for specific pathogens(CEN TC275/WG6/TAG3, K. Berghof)

• Detection of botulinum neurotoxin producing Clostridia

• First draft

• Real-Time PCR: Detection of neurotoxin genes type A, B, E and F

• Pathogenic Vibrio parahemolyticus

• Detection and enumeration on account of poor performance of cultural methods ISO 21871 and 21872

• toxR gene sequence R72H and hemolysin genes TDH and TRH

• Yersinia enterocolitica and pseudotuberculosis

• Investigation on enrichment


ELISA / Immunological methods(CEN TC275/WG6/Ad’hoc group, J. Hennekinne?)

• Necessity of General rules and guidelines

• Necessity for detection of staphylococcal enterotoxins


Primary production stage(CEN TC275/WG6/TAG5, R. Danguy des déserts)

Scope:• « From primary production (e.g. farm, natural environment) to

slaughter/harvest ».• The border would be live animals in the slaughter house• Samples taken after slaughter to indicate microbiological status of live animals

are included• Samples taken to indicate hugiene of meat are excluded

Work = 3 projects:

EN ISO/WD 6887-6Preparation of test samples, initial suspension and decimal dilutions for microbiological examination -- Part 6: Specific rules for the preparation of samples taken at the primary production stage

EN ISO/WD 13307 Primary production stage -- Sampling techniques

EN ISO/WD 10272-4 Primary production stage – Detection and enumeration of Campylobacter in samples from primary production

New area have to be investigated: “Fish and shellfish products”IAFP, Berlin, 200939/50

Quality management

40/50

Culture media(ISO/TC34/SC9/WG5 and CEN TC275/WG6/TAG1, M. Greenwood)

Revised EN ISO 11133-1 had been published in February 2009 again as a TS: Microbiology of food and animal feeding stuffs - Guidelines on preparation and production of culture media - Part 1 : general guidelines on quality assurance for the preparation of culture media in the laboratory. Joint standard water and food

� 2 parts of ISO 11133 will become 1 standard pr EN ISO 11133 (Preparation, production, storage and performance testing of culture media)Joint standard water and food

�Scope extended to reagents and confirmatory media


Validation( ISO/TC34 SC9/WG3 and CEN TC275/WG6/TAG2, P. In’t Veld)

- PG1: Terminology

- PG2: Validation of proprietary methods (quantitative and qualitative methods)

- PG3: Intermediate validation

- PG4: Method verification

- PG5: In-house validation (against and without a reference method)

- PG6: Technical requirements for establishment/revision of standardized methods


Validation( ISO/TC34 SC9/WG3 and CEN TC275/WG6/TAG2, P. In’t Veld)

Work – First draft:

�� NWIP EN ISO 16140-1 Terminology of Method Validatio n

�� NWIP EN ISO 16140-2 Protocol for the validation of alternative method – Part 2 : Protocol for the validation of propietary methods against a referenc e method

• Link with ISO TC34/SC9/WG2 “Statistics”

• Revision of EN ISO 16140:2003 Microbiology of food and animal feeding stuffs - Protocol for the validation of alternative methods

• Circulation of the draft of project of Amendment A1 of EN ISO 16410:2003 which correct errors in claus e 6.3.

• Extension of its scope to PCR and Immunological met hods

• EN ISO standards perhaps recognised by AOAC (Paul W EHLING, AOAC, Chairman Statistical Committee, new member of WG2.

• A full international validation process of microbio logical alternative method

• EN ISO 16140 and the life of reference method?


New concept of standards(i.e. chemistry)

Reference methods

Alternative methods

Validation, Equivalence

Performance criteria without description of method


European Mandate by EC for Validation of reference methods: 15 METHODS

1)1)1)1) Detection of foodDetection of foodDetection of foodDetection of food----borne viruses in food based on PCR techniqueborne viruses in food based on PCR techniqueborne viruses in food based on PCR techniqueborne viruses in food based on PCR technique””””2)2)2)2) Detection and quantification of histamineDetection and quantification of histamineDetection and quantification of histamineDetection and quantification of histamine3)3)3)3) Detection of staphylococcal enterotoxins for dairy products and Detection of staphylococcal enterotoxins for dairy products and Detection of staphylococcal enterotoxins for dairy products and Detection of staphylococcal enterotoxins for dairy products and other matricesother matricesother matricesother matrices4)4)4)4) Detection of Bacillus cereus enterotoxins in food (emetic and diDetection of Bacillus cereus enterotoxins in food (emetic and diDetection of Bacillus cereus enterotoxins in food (emetic and diDetection of Bacillus cereus enterotoxins in food (emetic and diarrheal toxin)arrheal toxin)arrheal toxin)arrheal toxin)5)5)5)5) Detection of Detection of Detection of Detection of Enterobacter sakazakiiEnterobacter sakazakiiEnterobacter sakazakiiEnterobacter sakazakii6)6)6)6) EN 21872EN 21872EN 21872EN 21872----1 Horizontal method for the detection of pathogenic 1 Horizontal method for the detection of pathogenic 1 Horizontal method for the detection of pathogenic 1 Horizontal method for the detection of pathogenic VibrioVibrioVibrioVibrio –––– Part 1: Detection of Part 1: Detection of Part 1: Detection of Part 1: Detection of Vibrio parahaemolyticus Vibrio parahaemolyticus Vibrio parahaemolyticus Vibrio parahaemolyticus andandandand

Vibrio choleraeVibrio choleraeVibrio choleraeVibrio cholerae7)7)7)7) EN 21872EN 21872EN 21872EN 21872----2 Horizontal method for the detection of pathogenic 2 Horizontal method for the detection of pathogenic 2 Horizontal method for the detection of pathogenic 2 Horizontal method for the detection of pathogenic VibrioVibrioVibrioVibrio –––– Part 2: Detection of species other than Part 2: Detection of species other than Part 2: Detection of species other than Part 2: Detection of species other than Vibrio Vibrio Vibrio Vibrio

parahaemolyticusparahaemolyticusparahaemolyticusparahaemolyticus and and and and Vibrio choleraeVibrio choleraeVibrio choleraeVibrio cholerae8)8)8)8) Horizontal methods for the detection and enumeration of EnterobaHorizontal methods for the detection and enumeration of EnterobaHorizontal methods for the detection and enumeration of EnterobaHorizontal methods for the detection and enumeration of Enterobacteriaceae cteriaceae cteriaceae cteriaceae –––– Part 1: Detection and enumeration by Part 1: Detection and enumeration by Part 1: Detection and enumeration by Part 1: Detection and enumeration by

MPN technique with preMPN technique with preMPN technique with preMPN technique with pre----enrichmentenrichmentenrichmentenrichment””””9)9)9)9) EN ISO 11290EN ISO 11290EN ISO 11290EN ISO 11290----1 Horizontal method for the detection and enumeration of 1 Horizontal method for the detection and enumeration of 1 Horizontal method for the detection and enumeration of 1 Horizontal method for the detection and enumeration of Listeria monocytogenesListeria monocytogenesListeria monocytogenesListeria monocytogenes –––– Part 1: Detection Part 1: Detection Part 1: Detection Part 1: Detection

methodmethodmethodmethod10)10)10)10) EN ISO 11290EN ISO 11290EN ISO 11290EN ISO 11290----2 Horizontal method for the detection and enumeration of 2 Horizontal method for the detection and enumeration of 2 Horizontal method for the detection and enumeration of 2 Horizontal method for the detection and enumeration of Listeria monocytogenesListeria monocytogenesListeria monocytogenesListeria monocytogenes –––– Part 2: Part 2: Part 2: Part 2:

Enumeration methoEnumeration methoEnumeration methoEnumeration metho11)11)11)11) EN ISO 16654 Horizontal method for the detection of EN ISO 16654 Horizontal method for the detection of EN ISO 16654 Horizontal method for the detection of EN ISO 16654 Horizontal method for the detection of E. coliE. coliE. coliE. coli O157O157O157O157””””12)12)12)12) EN ISO 10271EN ISO 10271EN ISO 10271EN ISO 10271----1 Horizontal method for the detection and enumeration of 1 Horizontal method for the detection and enumeration of 1 Horizontal method for the detection and enumeration of 1 Horizontal method for the detection and enumeration of CampylobacterCampylobacterCampylobacterCampylobacter spp. spp. spp. spp. –––– Part 1: Detection Part 1: Detection Part 1: Detection Part 1: Detection

methodmethodmethodmethod””””13)13)13)13) EN ISO 10272EN ISO 10272EN ISO 10272EN ISO 10272----2 Horizontal method for the detection and enumeration of 2 Horizontal method for the detection and enumeration of 2 Horizontal method for the detection and enumeration of 2 Horizontal method for the detection and enumeration of CampylobacterCampylobacterCampylobacterCampylobacter spp. spp. spp. spp. –––– Part 2: Colony count Part 2: Colony count Part 2: Colony count Part 2: Colony count

techniquetechniquetechniquetechnique””””14)14)14)14) EN ISO 6579/FDAmd 1 Horizontal method for the detection of EN ISO 6579/FDAmd 1 Horizontal method for the detection of EN ISO 6579/FDAmd 1 Horizontal method for the detection of EN ISO 6579/FDAmd 1 Horizontal method for the detection of SalmonellaSalmonellaSalmonellaSalmonella spp. spp. spp. spp. –––– Amendment 1 Annex D: Detection of Amendment 1 Annex D: Detection of Amendment 1 Annex D: Detection of Amendment 1 Annex D: Detection of

SalmonellaSalmonellaSalmonellaSalmonella spp. in animal faeces and in samples from the primary productiospp. in animal faeces and in samples from the primary productiospp. in animal faeces and in samples from the primary productiospp. in animal faeces and in samples from the primary production stagen stagen stagen stage15)15)15)15) EN ISO 10273 Horizontal method for the detection of presumptive EN ISO 10273 Horizontal method for the detection of presumptive EN ISO 10273 Horizontal method for the detection of presumptive EN ISO 10273 Horizontal method for the detection of presumptive pathogenic pathogenic pathogenic pathogenic Yersinia enterocoliticaYersinia enterocoliticaYersinia enterocoliticaYersinia enterocolitica


Proficiency testing(ISO TC34/SC9/WG4, S. Passmore)

Final vote: CEN ISO/TS 22117 “Proficiency testing”

Request for ILAC and EA recommending them to recognise this TS (Technical Specification) as technical requirements for proficiency testing organisation in the field of food microbiology (DIS ISO 17043).


Measurement uncertainty(ISO TC34/SC9/WG2, B. Lombard)

• ISO/TS 19036: measurement uncertainty• Amendment A1: low number

• New Work:• uncertainty of sampling in food microbiology,• maximum allowable values for measurement

uncertainty


News works or standards projects

Iso level: New project group: “Detection of contaminants in starter cultures and probiotics” (projecleader, S. Zuber, Switzerland)

Iso level: CEN ISO/TS 10272 – 3 Horizontal method for the detection and enumeration of Campylobacterspp - Part 3 – Semi-quantitative method

Iso level: ISO TS 2871 & 2 for vibrio: correction of the volume of culture transferred from first enrichment to second enrichment.

Iso level: Comparison of ISO 7251 to ISO 16649-3 Horizontal method for the detection and enumeration of presumptive Escherichia coli- Most probable number technique

ISo level: Horizontal method for the enumeration of microorganisms : EN ISO 4833

Poured plate colony count technique at 30 °°°°C Surface colony-count technique at 30 °°°°C

Iso level: Enumeration of Alicyclobacillus acidoterrestris

CEN level: Enterotoxin Bacillus cereus (P. in’t veld)

CEN level: Standardization of antimicrobial susceptibility analysis of foodborne pathogens

CEN level: Detection and quantification of histamine


Future for standardization of food microbiology

• Cover the food chain

• Integrate new technology

• Improve the publication delay of standards

• More flexible

• Avoid to put method inside regulation

• Financial support for ring trials !

• Description of method or standard based only on performance criteria?


Acknowlegments

50/50

Documents

Leclercq (Overview of standard methods development of ISO and CEN)