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R. Bras. Ci. Solo, 35:759-771, 2011 LEAST LIMITING WATER RANGE IN SOIL UNDER CROP ROTATIONS AND CHISELING (1) Juliano Carlos Calonego (2) & Ciro Antonio Rosolem (3) SUMMARY Soil water availability to plants is affected by soil compaction and other variables. The Least Limiting Water Range (LLWR) comprises soil physical variables affecting root growth and soil water availability, and can be managed by either mechanical or biological methods. There is evidence that effects of crop rotations could last longer than chiseling, so the objective of this study was to assess the effect of soil chiseling or growing cover crops under no-till (NT) on the LLWR. Crop rotations involving triticale (X Triticosecale) and sunflower (Helianthus annuus) in the fall-winter associated with millet (Pennisetum glaucum), sorghum (Sorghum bicolor) and sunn hemp (Crotalaria juncea) as cover crops preceding soybean (Glycine max) were repeated for three consecutive years. In the treatment with chiseling (performed only in the first year), the area was left fallow between the fall-winter and summer crops. The experiment was carried out in Botucatu, São Paulo State, Brazil, from 2003 to 2006 on a Typic Rhodudalf. The LLWR was determined in soil samples taken from the layers 0–20 cm and 20– 40 cm, after chemical desiccation of the cover crops in December of the first and third year of the experiment. Chiseling decreases soil bulk density in the 0–20 cm soil layer, increasing the LLWR magnitude by lowering the soil water content at which penetration resistance reaches 2.0 MPa; this effect is present up to the third year after chiseling and can reach to a depth of 0.40 m. Crop rotations involving sunflower + sunn hemp, triticale + millet and triticale + sunn hemp for three years prevented soil bulk density from exceeding the critical soil bulk density in the 0– 0.20 m layer. This effect was observed to a depth of 0.40 m after three years of chiseling under crop rotations involving forage sorghum. Hence, chiseling and some crop rotations under no tillage are effective in increasing soil quality assessed by the LLWR. Index terms: cover crops, crop rotation, soil compaction, no till, soil water. (1) Part of the PhD thesis of first author. Received for publication in July 2010 and approved in March 2011. (2) Center of Agricultural Sciences, UNOESTE, CEP 19067-175, Presidente Prudente, SP, Brazil. E-mail: [email protected] (3) Corresponding author, Department of Crop Science, College of Agricultural Sciences, São Paulo State University, Botucatu, SP, Brazil. E-mail: [email protected] Comissão 2.2 - Física do solo

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LEAST LIMITING WATER RANGE IN SOIL UNDER CROP ROTATIONS AND CHISELING 759

R. Bras. Ci. Solo, 35:759-771, 2011

LEAST LIMITING WATER RANGE IN SOIL UNDER CROP

ROTATIONS AND CHISELING(1)

Juliano Carlos Calonego(2) & Ciro Antonio Rosolem(3)

SUMMARY

Soil water availability to plants is affected by soil compaction and othervariables. The Least Limiting Water Range (LLWR) comprises soil physicalvariables affecting root growth and soil water availability, and can be managed byeither mechanical or biological methods. There is evidence that effects of croprotations could last longer than chiseling, so the objective of this study was toassess the effect of soil chiseling or growing cover crops under no-till (NT) on theLLWR. Crop rotations involving triticale (X Triticosecale) and sunflower(Helianthus annuus) in the fall-winter associated with millet (Pennisetumglaucum), sorghum (Sorghum bicolor) and sunn hemp (Crotalaria juncea) as covercrops preceding soybean (Glycine max) were repeated for three consecutive years.In the treatment with chiseling (performed only in the first year), the area was leftfallow between the fall-winter and summer crops. The experiment was carriedout in Botucatu, São Paulo State, Brazil, from 2003 to 2006 on a Typic Rhodudalf.The LLWR was determined in soil samples taken from the layers 0–20 cm and 20–40 cm, after chemical desiccation of the cover crops in December of the first andthird year of the experiment. Chiseling decreases soil bulk density in the 0–20 cmsoil layer, increasing the LLWR magnitude by lowering the soil water content atwhich penetration resistance reaches 2.0 MPa; this effect is present up to the thirdyear after chiseling and can reach to a depth of 0.40 m. Crop rotations involvingsunflower + sunn hemp, triticale + millet and triticale + sunn hemp for three yearsprevented soil bulk density from exceeding the critical soil bulk density in the 0–0.20 m layer. This effect was observed to a depth of 0.40 m after three years ofchiseling under crop rotations involving forage sorghum. Hence, chiseling andsome crop rotations under no tillage are effective in increasing soil quality assessedby the LLWR.

Index terms: cover crops, crop rotation, soil compaction, no till, soil water.

(1) Part of the PhD thesis of first author. Received for publication in July 2010 and approved in March 2011.(2) Center of Agricultural Sciences, UNOESTE, CEP 19067-175, Presidente Prudente, SP, Brazil. E-mail: [email protected](3) Corresponding author, Department of Crop Science, College of Agricultural Sciences, São Paulo State University, Botucatu,

SP, Brazil. E-mail: [email protected]

Comissão 2.2 - Física do solo

760 Juliano Carlos Calonego & Ciro Antonio Rosolem

R. Bras. Ci. Solo, 35:759-771, 2011

RESUMO: INTERVALO HÍDRICO ÓTIMO EM SOLO SOB ROTAÇÃO DECULTURAS E ESCARIFICAÇÃO

A disponibilidade de água às plantas é alterada pela compactação do solo e outrasvariáveis. O Intervalo Hídrico Ótimo (IHO) integra variáveis físicas do solo que alteram ocrescimento radicular e a disponibilidade de água e pode ser manejado por métodos mecânicosou biológicos. Há evidências de que os efeitos da rotação de culturas são mais duradouros queos da escarificação. O objetivo deste trabalho foi avaliar o efeito da escarificação e, ou, rotaçãode culturas em sistema de semeadura direta (SSD) no IHO. Rotações envolvendo triticale (XTriticosecale) e girassol (Helianthus annuus) no outono-inverno e milheto (Pennisetumglaucum), sorgo (Sorghum bicolor) e crotalária (Crotalaria juncea) com plantas de coberturaprecedendo a soja (Glycine max) foram repetidas por três anos. No tratamento com escarificação,a área foi deixada em pousio entre as culturas de outono-inverno e verão. O experimento foiconduzido em Botucatu, São Paulo, Brasil, de 2003 a 2006 em um Nitossolo Vermelho. OIHO foi determinado em amostras de solo das profundidades de 0–20 e 20–40 cm, logo após adessecação química das plantas de cobertura, em dezembro do primeiro e do terceiro ano doexperimento. A escarificação diminui a densidade do solo na camada de 0–20 cm, aumentandoo IHO por meio da redução da umidade do solo em que a resistência à penetração atinge2,0 MPa; esse efeito mantém-se até o terceiro ano após a escarificação, podendo chegar a até40 cm de profundidade. As rotações de culturas envolvendo girassol + crotalária, triticale +milheto e triticale + crotalária por três anos ajudaram a prevenir o aumento na densidade dosolo acima do valor crítico na camada de 0–20 cm. Esse efeito é observado na camada de 20–40 cm após três anos da escarificação e com rotações de culturas envolvendo sorgo. Assim, aescarificação e algumas rotações de culturas em sistema de semeadura direta são eficientes emmelhorar a qualidade do solo avaliada pelo IHO.

Termos de indexação: plantas de cobertura, rotação de culturas, compactação do solo, semeaduradireta, água no solo.

INTRODUCTION

The factors that affect plant growth and developmentdirectly (oxygen, water, temperature and mechanicalimpediment) are not only influenced by soil structure(bulk density, aggregation state and porosity), but alsoby climate, which vary from year to year (Dexter,1988). Thus, it is difficult to define critical limits beyondwhich crop growth and development is impaired(Torres & Saraiva, 1999). The recognition of theseinteractions suggests that soil physical quality shouldbe assessed using parameters that integrate thephysical characteristics of the soil affecting plantgrowth, and using water as an equilibrium variable,diminishing or aggravating the effects of aeration andsoil penetration resistance (Letey, 1985).

Plant growth is affected when soil physical-hydriccharacteristics such as aeration porosity, rootpenetration resistance and/or soil water contentimpairs water acquisition. The interaction of theseattributes in a single variable was defined by Silva etal. (1994) as the “Least Limiting Water Range”(LLWR). According to these authors, root growth isless restricted within this interval, whose upper limitis the soil water content at field capacity (FC) or thesoil water content when the air-filled porosity equals10 %. The LLWR lower limit is the soil water content

at the permanent wilting point (PWP) or the soil watercontent where soil penetration resistance is equal to2.0 MPa. There are reports showing that soilmanagement systems leading to lower LLWR exposecrops to more frequent stress due to excess or lack ofwater (Kay, 1990; Silva & Kay, 1996; Lapen et al.,2004).

Soil water contents at the adequate aerationporosity and soil penetration resistance (SR) are moreseverely affected by soil bulk density (Db) than by soilwater contents at FC and PWP, indicating that theLLWR is more sensitive to soil structural changesthan the available water (Silva et al., 1994; Tormenaet al., 1998; Betz et al., 1998; Zou et al., 2000). Thus,soil water contents to avoid limitations to plant growthvary with the state of soil compaction (Silva et al.,1994), and compaction alleviation may result in higherwater availability to plants in addition to decreasingSR.

Soil compaction alleviation can be achieved bymechanical and/or biological processes. In themechanical method, equipments with shanks arepreferred because they operate below the compactedlayer, have less surface contact and soil tillage is low(Vernetti Júnior & Gomes, 1999). However, there isevidence that the positive effects of chiseling are short-lived (Araujo et al., 2004; Busscher et al., 2002). On

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the other hand, important benefits have been observedin soil structure in the medium and long terms as aresult of cover crops (Fidalski et al., 2010) with high-carbon fixing potential and vigorous, deep root systems(Dias Júnior, 2000), able to grow through soil layerswith high resistance to penetration and creatingbiopores where the roots of the subsequent crop cangrow (Silva & Rosolem, 2001a). Black oat, pigeon peaand pearl millet favor soybean root growth belowcompacted soil layers (Silva & Rosolem, 2002) resultingin better mineral nutrition (Silva & Rosolem, 2001b),however, the effects on soil bulk density andpenetration resistance were not clear. Althoughgrasses are generally more sensitive to high soilpenetration resistance, they have high root lengthdensities in compacted soil layers. This was observedfor pearl millet and grain sorghum that are also veryefficient in recycling nutrients. These two speciesproved to be very appropriate as cover crops in tropicalregions with dry winters (Rosolem et al., 2002; Silva& Rosolem, 2003).

In view of the transient effect of chiseling, and thatthe positive effects of cover crops on root growth anddevelopment of cash crops as well as the effects ofcrop rotations on soil water availability are not wellunderstood, the objective of this study was to determinethe effects of soil compaction management withchiseling and no-till cover crops on the least limitingwater range of a tropical soil.

MATERIAL AND METHODS

An experiment was carried out in Botucatu, Stateof São Paulo, Brazil, from 2003 to 2006 on a TypicRhodudalf (Soil Survey Staff, 2006) or NitossoloVermelho distroférrico A moderado (Embrapa, 2006),in an area that had been under no tillage for six years,with a soybean/black oats/corn/triticale rotation. Thegeographic location of this area is latitude 22 ° 49 ’ S,

longitude 48 ° 25 ’ WGrw and 786 m asl. The climate(Köppen classification) is CWa, i.e., a subtropical humidclimate with dry winter. The dry season is well-defined between May and September. The meanannual rainfall is approximately 1,400 mm, thehighest monthly mean temperature is over 22 °C andthe lowest below 18 °C. Before starting the experiment,a one meter-deep trench was opened and a compactedlayer was diagnosed 5 to 20 cm deep in the soil profile.The soil was then sampled for chemical (Raij et al.,2001) and physical (Embrapa, 1997) analyses (Table 1).The soil resistance (SR) of the profile was determinedusing an electronic penetrograph.

Treatments consisted of planting triticale (XTriticosecale Wittmack) and sunflower (Helianthusannuus) in the fall-winter, combined with pearl millet(Pennisetum glaucum L., var. BN-2), forage sorghum(Sorghum bicolor (L.) Moench) and sunn hemp(Crotalaria juncea L.) in the spring, and an additionaltreatment that was chiseled, all preceding the summercrop (soybean – Glycine max L. (Merrill), var.Embrapa 48). The chisel plow had seven shanks ontwo parallel bars on a square tool carrier. The shanks,inclined 25° forward, were fixed 0.60 m apart,resulting in an effective between-shank spacing of0.30 m, with a maximum depth of 0.30 m. A clod-breaking roller was attached to break the biggest clods,decrease surface roughness and spare disking.Soybean was grown in summer in all plots. The cropsequence was repeated for three years. The soil waschiseled only once, that is, after the fall-winter cropharvest in the first year, and the chiseled plots wereleft fallow between the winter and summer cropsthereafter. The experiment was evaluated in acomplete randomized block design with a split-plotarrangement of treatments, with four replications.Triticale and sunflower were grown in the plots inthe fall/winter with pearl millet, sorghum, sunn hemp,and chiseling in sub-plots. Plots were 8.0 x 32.0 m,andsub-plots 8.0 x 5.0 m, and blocks, plots and sub-plotswere 4.0 m apart.

Table 1. Chemical and physical properties of the soil in the experimental area

(1) pH determined in CaCl2 0.01 mmol L-1. (2) Soil mechanical penetration resistance. (3) Soil gravimetric moisture and Db at thetime of the penetration resistance test. (4) Macroporosity drained at a matric potential of -0.006 MPa.

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Soybean fertilization with P and K followed specificrecommendations for the crop according to Raij et al.(1996). Sunflower and triticale were not fertilized andthe cover crops (in spring) received 40 kg ha-1 N asurea at planting.

Undisturbed soil samples were collected withvolumetric rings (height 5.0 cm, internal diameter4.8 cm), and 16 samples were taken per plot (8 fromthe center of the 0–20 cm and 8 from the center of the20–40 cm layer) before starting the experiment tocharacterize the area, and after desiccating the covercrops in the first and third year of the experiment(just before soybean planting).

The LLWR was determined as described by Silvaet al. (1994). The least limiting water range limitswere associated to critical soil volumetric watercontent (θ) defined by the matric potential, soilpenetration resistance, and air-filled porosity asfollows: the soil water retention at field capacity (θFC),calculated at -0.01 MPa matric potential, the soil waterretention at permanent wilting point (θPWP), -1.5 MPamatric potential, the soil water content (θSR) at whichpenetration resistance reaches 2.0 MPa, and the soilwater content when the air-filled porosity (θAFP) equals10 %.

In the laboratory, all samples were, at first, water-saturated (Embrapa, 1997) and submitted to thefollowing tensions: 0.002, 0.004, 0.006, 0.01, 0.03, 0.1,0.5, and 1.5 MPa. A tension table was used to obtainmatric potentials (Ψ) of -0.002, -0.004 and -0.006 MPa.For Ψ of -0.01, -0.033, -0.1, -0.5, and -1.5 MPa sampleswere placed on a porous plate apparatus. When thesamples reached equilibrium, penetration resistancewas determined and samples were oven-dried at 105 °Cfor 24 h to determine gravimetric moisture and soilbulk density (Embrapa, 1997). The penetrograph(Marconi-model MA 933) consisted of a 6 mmdiameter metal rod with a 30° half-angle conical tipand base area of 0.1256 cm² attached to a charge cellheld by a mechanical arm driven by a cork screw-likeaxle. The vertical displacement speed of the rod was1.0 cm min-1 to the depth of 4.0 cm. Data obtainedfrom the sample surface to 1.0 cm deep were discarded,as recommended by Tormena et al. (1998). Thevolumetric moisture (θ) was obtained by multiplyinggravimetric moisture by soil bulk density. The θFCand θPWP values were determined using equation 1(Silva et al., 1994).

θ = exp (a + b Db) Ψc (1)

where θ = volumetric water content (m3 m-3); Db =bulk density (g cm-3); Ψ = matric potential (MPa); a,b, c = calibration coefficients.

The critical value of volumetric moisture belowwhich SR is greater than 2.0 MPa (θSR) was obtainedby the functional relationship between SR, θ and Dbas in equation 2.

SR = dθe Dbf (2)

where SR = Soil penetration resistance (MPa); θ =volumetric water content of the sample (m3 m-3); Db =soil bulk density (g cm-3); d, e, f = calibration coefficients.

Finally the θPA was obtained by equation 3.

θAFP = θSAT – 0.1 (3)

where θAFP = soil water content where aerationporosity is equal to 10 % (0.1 m3 m-3); θSAT = (1-Db/2.65), and Db is the soil bulk density value (g cm-3)and 2.65 (g cm-3) is the assumed value of the meanparticle density.

Thus, the upper limit (UL) of the LLWR isdetermined by the lower value of either θFC or θAFP,the lower limit (LL) of the LLWR equals the highervalue of either θSR or θPWP, and LLWR is calculatedas LLWR = UL – LL. After calculating the upperand lower LLWR limits, the critical soil bulk density(Dbc) was determined, i.e., the soil density at whichLLWR equals zero, (Silva et al., 1994).

The statistical software Sigma Plot 8.0 was usedto fit equations 1 and 2 and calculate the coefficientsa, b, c, d, e, f (considered significant when p < 0.05)with individual data fit for each treatment and depth.The upper and lower LLWR limits as a function ofsoil bulk density were determined using a XML-basedelectronic spreadsheet, as described by Leão & Silva(2004).

RESULTS AND DISCUSSION

The determination coefficients of equations 1 and2 had significant F values with p < 0.01 (Tables 2 and3). In the first year of the experiment, differences inLLWR were small in all treatments and both soillayers (Figures 1 and 2) because the lower soil watercontent limit was very close to the upper limit in allsamples. Chiseling led to a higher LLWR amplitudein the 0–20 cm layer (Figure 1d,h), which was notobserved for the 20–40 cm layer (Figure 2d,h).According to Carter et al. (1999), the LLWR in NT islower than in conventional tillage because SR reaches2.0 MPa at higher moisture contents in the formersystem, due to the higher Db usually found in theconservation system. Betz et al. (1998) and Cavalieriet al. (2006) also observed greater LLWR in soil underconventional tillage and chiseling, resulting in afavorable physical environment for root growth.Regardless of soil depth, the water content bringingSR to 2 MPa (θRP) equaled the lower LLWR limit inall samples, and was higher than θPWP irrespectiveof Db in the first year. This result is typical ofcompacted soils that require high moisture to keepSR below the limiting value (Topp et al., 1994; Silvaet al., 1994; Muller, 2002; Beutler et al., 2006).

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Table 3. Estimation of regression coefficients for the volumetric water content (θθθθθ) as a function of soil bulkdensity (Db) and matric potential (ΨΨΨΨΨ), in the 0–0.20 and 0.20–0.40 m layers, in the first and third year ofthe experiment

Significant at p < 0.01.

Table 2. Estimation of regression coefficients for the soil penetration resistance (SR) as a function ofvolumetric water content (θθθθθ) and soil bulk density (Db), in the 0–0.20 and 0.20–0.40 m layers, in the firstand third year of the experiment

Significant at p < 0.01.

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In all treatments and soil layers the availablewater content (AW = θFC–θPWP) was higher thanLLWR in the first year of the experiment, which istypical of degraded soils (Letey, 1985). For this reason,Silva et al. (1994) suggested the LLWR as a moresensitive tool for soil structure analyses. According

to Topp et al. (1994), the occurrence of very low, oreven zero LLWR values is common in soils with heavyclay texture, as in this study (Table 1), whichincreases the probability of physical limitations forroot growth (Kay, 1990). Tormena et al. (1998), whendetermining the LLWR in a Red Latosol with

Figure 1. Soil water content (θθθθθ) as affected by soil bulk density, at the critical levels of field capacity (FC =-0.01 MPa), permanent wilting point (PWP = -1.5 MPa), air-filled porosity (AFP = 10 %) and soil penetrationresistance (SR = 2MPa). Samples collected in the 0–20 cm layer, in the first year of the experiment. Theshaded area represents the LLWR. (a) sunflower+millet; (b) sunflower+forage sorghum; (c)sunflower+sunn hemp; (d) sunflower+chiseling; (e) triticale+millet; (f) triticale+forage sorghum; (g)triticale+sunn hemp; (h) triticale+chiseling.

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800 g kg-1 clay, observed that the θSR was the lowerlimit of soil available water, with Db > 1.10 g cm-3

and soil moisture over 0.24 cm cm-3. This was notobserved in the present experiment because in mosttreatments the lowest Db was higher than 1.10 g cm-3

and θSR higher than 0.34 g cm-3, especially in the 20–40 cm layer, which contains more clay. Even in thechiseled treatments (Figure 1d,h), where the lowestDb were 1.05 and 1.06 g cm-3 under sunflower +

chiseling and triticale + chiseling, respectively, theθSR represented the lower LLWR limit. Thus, it canbe inferred that shortly after chiseling, thismanagement was not efficient in increasing the LLWRto reach the values similar to those reported in theliterature by Tormena et al.(1998), because at the sameDb (of around 1.10 g cm-3) the θSR was much higher,that is, 0.35 g cm-3. However, after growing covercrops with vigorous, aggressive root systems under

Figure 2. Soil water content (θθθθθ) as affected by soil bulk density, at the critical levels of field capacity (FC =-0.01 MPa), permanent wilting point (PWP = -1.5 MPa), air-filled porosity (AFP = 10 %) and soil penetrationresistance (SR = 2MPa). Samples collected in the 20–40 cm layer, in the first year of the experiment. Theshaded area represents the LLWR. (a) sunflower+millet; (b) sunflower+forage sorghum; (c)sunflower+sunn hemp; (d) sunflower+chiseling; (e) triticale+millet; (f) triticale+forage sorghum; (g)triticale+sunn hemp; (h) triticale+chiseling.

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NT the limiting SR for plant growth may be higherthan under conventional systems. This is possiblebecause when roots die they leave biopores within thesoil profile allowing new root growth in depth (Silva& Rosolem, 2001b).

Regarding the upper limit of LLWR, the θFC wasthe maximum limit of soil water in all samples.Exceptions were the chiseling treatments in the 0–20 cm layer, where θAFP represented the upper limitof LLWR at Db values higher than 1.28 and1.27 g cm-3 under sunflower and triticale, respectively.At these Db values the moisture for 10 % air-filledporosity (AFP) was approximately 0.42 cm cm-3. TheθAFP was the upper LLWR limit in 9 and 25 %,respectively, of the samples within the LLWR in thechiseled plots with triticale and sunflower as fall-wintercrops. The replacement of θFC by the θAFP as the upperlimit of IHO is common in clayey soils, due to the lowsoil macroporosity, as in this study (Table 1).Tormena et al. (1998) obtained similar results in aclayey soil, that is, the θAFP became the upper LLWRlimit, substituting the θFC, with Db of 1.28 g cm-3,with a soil volumetric moisture of around 0.42 cm cm-3.However, Pereira et al. (2010) did not observe θAFP asthe upper limit of LLWR for all Db values obtained,due to a high sand content (642 g kg-1).

In the two chiseled treatments with sunflower ortriticale in the fall-winter (Figure 1d,h), the Dbc werethe highest in the first year, 1.31 and 1.33 g cm-3

respectively. This occurs due the large number ofsoil fractures, helping to maintain the pre-establishedcondition of SR at 2.0 MPa, even with the increase ofDb, increasing the Dbc. These Dbc values were similarto those reported by Müller (2002) in a Red Nitossol(Haplortox) with 590 g cm-3 clay in the 10–20 cm layer(Dbc = 1.33 g cm-3), and higher than the 1.28 g cm-3

Dbc reported by Tormena et al. (1998) in a Red Latossolwith 800 g cm-3 clay in the 0 to 10 cm layer. Cavalieriet al. (2006) observed critical Db in treatments withmechanical management, decreasing the percentageof samples with Db > Dbc. For Klein & Câmara(2007), sporadic sub-soiling in areas under no tillageresult in favorable soil physical conditions for plantdevelopment, specifically by reducing the soilpenetration resistance.

In the 20–40 cm layer LLWR was generally lowerthan in the 0–20 cm layer (Figure 2), due to therestrictive effect of the SR that reached the 2.0 MPalimit value at high moisture contents, even at thelowest Db (θSR ≥ 0.40 cm cm-3). This effect supportsfindings by Tormena et al. (1998) who predicted agreater impact of SR on LLWR deeper in the soilprofile. There was no effect of chiseling on LLWR inthis layer, probably because it was the deepest limitof action of the equipment (20–40 cm layer) andtherefore the de-compaction effect occurred only downto the tip of each shank. Araújo et al. (2004) alsoobserved smaller differences in deeper Db in acomparison of NT with and without sub-soiling.

In the third year of the experiment, LLWRincreased in the 0–20 cm layer in all treatments(Figure 3). However, the lowest LLWR limit was stillrepresented by θSR, except under triticale + chiselingwhere, at the lowest Db values (1.02 and 1.04 g cm-3),the LLWR was determined by the available water,that is, by θFC and θPWP, which is typical of a soilprofile that is less restrictive to root development. Inthis layer, chiseling led to the greatest LLWRamplitude, especially in plots with winter triticale(Figure 3d,h), where the variation in Db values wasgreatest. The Db values were lowest under sunflower+ chiseling and triticale + chiseling (1.09 and1.02 g cm-3, respectively), and Db values high (1.42and 1.49 g cm-3, respectively), which resulted in ahigher coefficient of variation (7.15 and 10.08 %,respectively). Cavalieri et al. (2006) also observed theeffect of mechanical implements on the increase ofdispersion in Db values as soil mobilization increased.

In the 20–40 cm layer the LLWR amplitude wasgreater (Figure 4) under rotations involving foragesorghum (Figure 4b,f) and chiseling (Figure 4d,h).However, after three seasons, there was an increasein Dbc in most plots, both in the 0–20 cm (Figure 5a)and in the 20–40 cm layer (Figure 5b). The exceptionswere triticale+chiseling in the 0–20 cm layer, whereDbc remained at 1.33 g cm-3 and sunflower + milletin the 20–40 cm layer where the LLWR equaled zero.

The highest Dbc was observed in the 0–20 cm layerafter three years of sunflower as fall-winter crop andsunn hemp as cover crop preceding soybean(Figure 5a). This treatment resulted in a Dbc of1.37 g cm-3, since the increase required in soilvolumetric water content to maintain SR at 2.0 MPawas low, due to an increase in the Db (Figure 3c). AsDbc was very high, 97 % of the samples collected todetermine the LLWR had Db < Dbc (Figure 6a), i.e.,a reduced probability of occurrence of highly restrictiveconditions to plant growth (Silva & Kay, 1997).

Crop rotation of sunflower and sunn hemp for threeconsecutive years resulted in the highest soil organicmatter (SOM) contents in the 0–20 cm layer (Calonego& Rosolem, 2008), with a beneficial influence on thesoil structure (Mielniczuk, 1999; Zonta et al., 2006)and consequently on SR reduction. According toSharma & Bhushan (2001), biomass addition to thesoil increases θAFP and decreases θSR, resulting in ahigher LLWR.

High frequencies of samples with Db < Dbc werealso observed under triticale + millet and triticale +sunn hemp (94 and 91 %, respectively) in the 0–20 cmlayer. Considering the Dbc values as a tool to assesstreatment effects on soil structural quality, it can bestated that introduction of cover crops in the systemled to higher Dbc increases in the 0–20 cm soil layer,with values similar to or even higher than thoseobserved in plots with mechanical intervention(Figure 5a). Thus, it can be inferred that biologicalsub-soiling, rather than mechanical chiseling, would

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result in increasingly favorable soil physicalcharacteristics in the future, mainly when soil organicmatter is increased. To increase SOM the systemmust include not only grasses, but also legumes, suchas sunn hemp in the present experiment, able to fixnitrogen. Moreover, similar results could be expectedin other soils and regions with similar climate,

although the time of response may be different,depending on the soil texture. However, a newchiseling in NT and fallow areas between the fall-winter and summer crops would be required. This issupported by the results presented in figure 6a in thetriticale+chiseling treatment, where the percentageof samples with Db < Dbc in the 0–20 cm layer,

Figure 3. Soil water content (θθθθθ) as affected by soil bulk density, at the critical levels of field capacity (FC =-0.01 MPa), permanent wilting point (PWP = -1.5 MPa), air-filled porosity (AFP = 10 %) and soil penetrationresistance (SR = 2MPa). Samples collected in the 0–20 cm layer, in the third year of the experiment. Theshaded area represents the LLWR. (a) sunflower+millet; (b) sunflower+forage sorghum; (c)sunflower+sunn hemp; (d) sunflower+chiseling; (e) triticale+millet; (f) triticale+forage sorghum; (g)triticale+sunn hemp; (h) triticale+chiseling.

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decreased from 88 to 56 % between the first and thirdyear of the experiment. These results explain thedecrease in soybean yield with this mechanicalmanagement, compared with plots under crop rotation(Calonego & Rosolem, 2010). In contrast, in the 20–40 cm layer, although the Dbc values increased inmost treatments with cover crops, the values still

remained lower than in chiseled plots (Figure 5b). Forthis reason, chiseling resulted in the highest frequencyof samples (63 and 69 %) with Db < Dbc (Figure 6),corroborating results of Cavalieri et al. (2006), whoobtained a sample frequency of 56 % with Db < Dbcin chiseled soils, against 25 % in treatments withoutmechanical intervention, in the 15–30 cm layer.

Figure 4. Soil water content (θθθθθ) as affected by soil bulk density, at the critical levels of field capacity (FC =-0.01 MPa), permanent wilting point (PWP = -1.5 MPa), air-filled porosity (AFP = 10 %) and soil penetrationresistance (SR = 2MPa). Samples collected in the 20–40 cm layer, in the third year of the experiment.The shaded area represents the LLWR. (a) sunflower+millet; (b) sunflower+forage sorghum; (c)sunflower+sunn hemp; (d) sunflower+chiseling; (e) triticale+millet; (f) triticale+forage sorghum; (g)triticale+sunn hemp; (h) triticale+chiseling.

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CONCLUSIONS

1. Chiseling decreases soil bulk density in the 0–20 cm soil layer, increasing the LLWR magnitude bylowering the soil water content at which penetrationresistance reaches 2.0 MPa; this effect is measurableuntil the third year after chiseling, to a depth of 0.40 m.

2. Crop rotations with sunflower + sunn hemp,triticale + millet and triticale + sunn hemp for threeyears prevented soil bulk density from exceeding thecritical bulk density in the 0–0.20 m layer. However,this effect is observed to a depth of 0.40 m,after threeyears of chiseling and crop rotations involving foragesorghum.

3. Chiseling and some crop rotations under notillage are effective in increasing soil quality asassessed by the LLWR.

ACKNOWLEDGEMENTS

This research was funded by FAPESP (researchfoundation of the State of São Paulo) and CNPq(National Council for Scientific and TechnologicalDevelopment).

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