KEMENTERIAN PENDIDIKAN DAN KEBUDAYAAN
FAKULTAS PERTANIAN UNIVERSITAS SEBELAS MARET
PROGRAM STUDI ILMU DAN TEKNOLOGI PANGAN
Jl. Ir. Sutami 36A Kentingan Surakarta Telp./Fax. (0271)
637457
LAPORAN SEMENTARA PRAKTIKUM FERMENTASIPENERAPAN KINETIKA
MIKROBIA DALAM FERMENTASI ASAM LAKTAT PADA MEDIA AIR KELAPA DAN
EKSTRAK TAUGENama anggota:
1. Bintang Ramadhan
2. Dhita Eka Rizki
3. Gagah Analdi
4. Nur Hidayanti
5. Siti MardiyahA. Penentuan Jumlah Sel dengan Metode
Perhitungan HemacytometerTabel 1.1 Penentuan Jumlah Sel Koloni A
dengan Metode Perhitugan HemacytometerJam ke-Jumlah selRata2 Jumlah
MoFPJumlah Sel/MlLog jumlah sel/ml
028256.411.4E+077.149219
0.527755.411.4E+077.14145
135771.411.8E+077.251638
1.516733.418.4E+066.921686
210621.215.3E+066.724276
2.57815.613.9E+066.591065
38116.214.1E+066.607455
417635.218.8E+066.944483
53006011.5E+077.176091
624148.211.2E+077.080987
72154311.1E+077.031408
822144.211.1E+077.043362
931963.811.6E+077.202761
1029659.211.5E+077.170262
1123747.411.2E+077.073718
12641128.213.2E+077.505828
1318937.8109.5E+077.975432
1416232.418.1E+066.908485
1526252.4101.3E+088.117271
1618937.8109.5E+077.975432
1719639.2109.8E+077.991226
1813026106.5E+077.812913
1917034108.5E+077.929419
2012925.8106.5E+077.80956
21673134.6103.4E+088.526985
221132226.4105.7E+088.752816
238316.61004.2E+088.618048
246112.21003.1E+088.4843
Gambar 1.1 Grafik Perhitungan Jumlah Sel Koloni A dengan
Hemacytometer
Tabel 1.2 Penentuan Jumlah Sel Koloni B dengan Metode Perhitugan
HemacytometerJam ke-Jumlah SelRata2 Jumlah MoFPJumlah Sel/MlLog
jumlah sel/ml
0797159.4104.0E+088.60042833
0.51303260.6106.5E+088.81391442
11006201.2105.0E+088.70156799
1.544188.2102.2E+088.34340859
2627125.4103.1E+088.49623755
2.536472.8101.8E+088.26007139
3680136103.4E+088.53147892
4795159104.0E+088.59933713
51124224.8105.6E+088.74973632
61133226.6105.7E+088.75319991
733066101.7E+088.21748394
8966193.2104.8E+088.68394713
934168.2101.7E+088.23172438
1031663.2101.6E+088.19865709
1143987.8102.2E+088.34143452
1232765.4101.6E+088.21351776
1330360.6101.5E+088.18041263
1455111002.8E+088.43933269
1530260.41001.5E+099.17897695
16290581001.5E+099.161368
1719438.8109.7E+077.98677173
1826052101.3E+088.11394335
196312.61003.2E+088.49831055
205911.81003.0E+088.46982202
2128256.41001.4E+099.14921911
2227454.81001.4E+099.13672057
23290581001.5E+099.161368
2437975.81001.9E+099.27760921
Gambar 1.2 Grafik Perhitungan Jumlah Sel Koloni B dengan
HemacytometerB. Penentuan Kadar Glukosa (Metode Nelson Somogyi)
Mencari kadar gula reduksi
Kadar gula reduksi terlarut (mg)=Konsentrasi larutan glukosa
standar x volume larutan glukosa standar yang dipakai
0 ml=0
0,2 ml=0.037
0,4 ml=0.074
0,6 ml=0.111
0,8 ml=0.149
1 ml=0.186
Tabel 2.1 Hasil Absorbansi Larutan Glukosa Standar2,6 mg/14
ml
Larutan Glukosa Standar (ml)Aquades (ml)Kadar Gula Reduksi
Terlarut (mg)Absorbansi ()
010.0000.093
0.20.80.0370.231
0.40.60.0740.426
0.60.40.1110.598
0.80.20.1490.678
100.1860.776
Gambar 2.1 Kurva Standar Hubungan Kadar Gula Reduksi Terlarut
(x) dengan Absorbansi (y)
2.1 Ekstrak Tauge
Tabel 2.1.1 Data Absorbansi Kadar Gula Reduksi (Media: Air
Kelapa dan Ekstrak Tauge
Waktu (jam)FPAbsorbansixKadar Gula Reduksi (%)
02500.2720.0411.0356
0.52501.7640.43510.8773
12500.5250.1082.7045
1.52502.8720.72718.1860
22500.8180.1854.6372
2.52501.3720.3328.2916
32501.2460.2987.4582
42500.4490.0882.2032
52501.3860.3358.3839
62501.9270.47811.9525
72502.3820.59814.9560
82501.7550.43310.8179
92502.7990.70817.7045
102501.6460.40410.0989
112500.9480.2205.4925
122500.3420.0601.4974
132500.7910.1784.4569
142500.4720.0942.3549
152500.2760.0421.0620
162500.2580.0380.9433
172500.2330.0310.7784
182500.6190.1333.3245
192500.4280.0832.0646
202500.2250.0290.7278
212500.1190.0010.0264
222500.1560.0110.2704
232500.2140.0260.6508
242500.1650.0130.3298
Persamaan regresi kurva standar
y =3,790x + 0,115
a =0.115
b =3.790
Sampel (ml)1ml
Sampel (mg)1000
FP =250
100
Rumus : Kadar gula reduksi (%) = (x*FP*100)/mg sampel
Gambar 2.1.1 Kurva Absorbansi Kadar Gula Reduksi (Media: Air
Kelapa dan Ekstrak Tauge)
2.2 Ammonium SulfatTabel 2.2.1 Data Absorbansi Kadar Gula
Reduksi (Media: Air Kelapa dan Ammonium Sulfat)Waktu
(jam)FPAbsorbansiXKadar Gula Reduksi (%)
02500.3990.0751.8755
0.52503.6120.92323.0673
12500.7310.1634.0633
1.52502.1230.53013.2476
22500.7750.1744.3558
2.52502.7370.69217.2955
32502.5720.64816.2049
42502.1810.54513.6280
52503.1350.79719.9208
62502.9300.74318.5686
72501.9560.48612.1438
82502.2140.55413.8478
92501.6440.40410.0880
102502.1980.55013.7379
112501.8960.47011.7480
122500.2930.0471.1741
132500.7720.1734.3338
142500.3490.0621.5435
152500.4060.0771.9173
162500.3420.0601.4974
172500.1740.0160.3892
182500.3860.0721.7876
192500.6240.1343.3553
202500.4580.0912.2625
212500.8330.1894.7361
222500.2670.0401.0026
232500.5710.1203.0101
242500.4670.0932.3219
Persamaan regresi kurva standar
y =3,790x + 0,115
a =0.115
b =3.790
Sampel (ml)1ml
Sampel (mg)1000
100
FP =250
Rumus : Kadar gula reduksi (%) = (x*FP*100)/mg sampel
Gambar 2.2.1 Kurva Absorbansi Kadar Gula Reduksi (Media: Air
Kelapa dan Ammonium Sulfat)
C. Penentuan Kadar Asam Laktat (dengan Titrimetri)
3.1.Media ZA
Tabel 3.1.1 Penentuan Kadar Asam Laktat dengan Media ZA
Jam Keml NaOHJam KeKadar AL (%)
00.200.0018
0.50.30.50.0027
10.410.0036
1.50.41.50.0036
20.420.0036
2.50.32.50.0027
30.330.0027
40.840.0072
50.350.0027
60.260.0018
70.270.0018
80.380.0027
90.490.0036
100.5100.0045
110.5110.0045
120.4120.0036
130.4130.0036
140.3140.0027
150.4150.0036
160.4160.0036
170.3170.0027
180.4180.0036
190.3190.0027
200.4200.0036
210.5210.0045
220.5220.0045
230.5230.0045
240.5240.0045
N NaOH= 0,1
BM Asam Laktat= 90
ml sampel = 1
Gambar 3.1.1 Penentuan Kadar Asam Laktat dengan Media ZA3.2Media
Ekstrak Tauge
Tabel 3.2.1 Penentuan Kadar Asam Laktat dengan Media Ekstrak
Tauge
Jam Keml NaOHJam KeKadar AL (%)
00.400.0036
0.50.50.50.0045
10.410.0036
1.50.41.50.0036
20.520.0045
2.50.62.50.0054
30.430.0036
40.540.0045
50.650.0054
60.960.0081
70.870.0072
80.680.0054
90.490.0036
100.6100.0054
110.7110.0063
120.5120.0045
130.8130.0072
140.7140.0063
150.6150.0054
160.6160.0054
170.9170.0081
180.6180.0054
190.8190.0072
200.6200.0054
210.9210.0081
220.8220.0072
230.7230.0063
240.5240.0045
N NaOH= 0,1
BM Asam Laktat= 90
ml sampel = 1
Gambar 3.2.1 Penentuan Kadar Asam Laktat dengan Media Ekstrak
Tauge
