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7/21/2019 Labs and Dx Vmmc Sepsis
1/21
DIAGNOSTIC PROCEDURES
Cytologicand
Hystologic Studies
Cytological studies indicate superficial soft tissue masses/inflammatory lesions, Intra-abdominal masses, Peripheral andmesenteric lymph nodes, Internal organs, Body cavity effusions. Histology Indicates firm lesions, iagnoses !here architecture
is needed to ma"e the diagnosis #e.g. small cell lymphoma, inflammation in hemodiluted samples$
Date Done Name of Test/Pocedue
Indication fo t!etest / Pocedue
Nomal"indings
Actual Results Inte#etation andAnalysis
NusingRes#onsi$ilities
%ctober&', &'(&
%AmangRodigue&
'edicalCente(
Biopsy
)ppendectomy
A##endi)
) diagnosticprocedure used to
investigatesuperficial lumps or
masses. It is a
method of obtainingdiagnostic materialfor cytologic #cell$
and histologic#tissue$ study thatcauses a minimal
amount of trauma tothe patient. (Manual
of Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page 763
Benign ornegative* no
abnormalcells or
abnormal
tissuepresent
+opathogenicorganisms
he specimenconsists ofvermiform
appendi . '.0 '.1 cm.
he serosa isdar" bro!n andcovered !ithcream-yello!
purulenteudates.2ectioning
reveals a '.( cmthic" !all bearing
a '. cm in
diameter point ofrupture at itsdistal third
segment. helumen averages
'.3 cm indiameter andcontains dar"bro!n fecal
Inte#etation*
)n abnormal result ofthe biopsy may be due
to
Analysis*
(Manual of Laboratory& Diagnostic Tests byFischbach, 7th Edition,
page76!
Petest Patient Cae
4plain the purpose and
procedure, benefits, andris"s of the test. 4venthough a local
anesthetic is used, theprocedure causes somediscomfort, and thisshould not beminimi5ed. If theapproach involvespassing near a rib, thepain may be greaterbecause of thesensitivity of the bone6
this is not a cause foralarm.
7nepected pain may
induce a vasovagal orother undesirableresponse. %ther ris"sinclude infection andhematoma or
7/21/2019 Labs and Dx Vmmc Sepsis
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material. Bloc" 8
)cute2uppurative
appendicitis,ruptured, !ithperiappendicitis
hemorrhage, dependingon the site aspirated.
Pocedue
7se local anesthesia in
most cases. )spiratesuperficial or palpablelesions !ithoutradiologic aid, butaspirate nonpalpablelesions usingradiographic imaging asan aid for needleplacement. 7se sterile
techni9ue. Position the needle
properly, then retractthe plunger of thesyringe to createnegative pressure.:ove the needle up anddo!n, and sometimesat several differentangles. ;elease the
plunger of the syringeand remove the needle.
4press material
obtained onto glassslides, !hich musteither be fiedimmediately in 0ro!th* =ightgro!th
Inte#etation*)n abnormal result ofthe pathology may be
due to the
Analysis*(Medical and "urgical
#ursing by$gnata%icius and
or'an, )th Edition,page *+7
+ovember&, &'(8 ,ounddisc!age It is a method ofobtaining diagnosticmaterial for cytologic#cell$ and histologic#tissue$ study thatcauses a minimal
amount of trauma tothe patient. (Manual
of Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page 763
Benign ornegative* noabnormalcells or
abnormaltissue
present
+opathogenic
organisms
Clinicalimpression*2urgical siteimpression
?inal reading*Candida)lbicans
;emar"s* >erm
tube result
Inte#etation*)n abnormal result ofthe pathology may be
due to the
Analysis*(Medical and "urgical
#ursing by$gnata%icius and
or'an, )th Edition,
page *+7
7/21/2019 Labs and Dx Vmmc Sepsis
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:onitor for signs of
inflammation and usesite care infectioncontrol measures. reat
pain, !hich may becommon in sensitiveareas such as thebreast, nipple, prostate,and scrotum.
:onitor for specific
problems, !hich varydepending on the siteaspirated #eg,hemoptysis after a lung
aspiration$. Counsel about follo!-up
procedures forinfections and malignantconditions.
7/21/2019 Labs and Dx Vmmc Sepsis
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He#atitis -Seology
Test
his test is an indication of previous infection, as !ell as current infection, and gives an indication of immunity #antibodies$ tofuture eposure, in the case of Hepatitis B.
Date Done Name of Test/Pocedue
Indication fo t!etest / Pocedue Cut off +alue Patient +alue Inte#etation andAnalysis Nusing es#onsi$ilities
%ctober &,&'(8
Hepatitis Bsurfaceantigen
#HBs )g$
o screen for anddetect HB@
infections6 earliestindicator of acutehepatitis B and
fre9uently identifies
infected peoplebefore symptomsappear
(Manual ofLaboratory &
Diagnostic Tests byFischbach, 7th
Edition, page ))+
+on-reactive +on-reactive Nomal Pre-test*
)ssess patientAs social
and clinical history and"no!ledge of test.4plain test purposeand procedure.
Procedure*
Collect a 1-m= blood
serum sample in a red-topped tube or t!olavender-toppedethylenediaminetetraacetic acid #4)$ tubes, m= each, for plasma.%bserve standard
precautions. Centrifugepromptly andaseptically. Placespecimen in abioha5ard bag fortransport to thelaboratory. 2endspecimens fro5en on
%ctober &,&'(8 Hepatitis Bantibody#)nti-Hbs$
his test indicatesthat a person hassuccessfully
responded to thehepatitis B vaccineor has recovered
from an acutehepatitis B
infection. (Manual
C.%.@.('.''' 0.81;eactive +ormal
7/21/2019 Labs and Dx Vmmc Sepsis
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of Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page )-*
dry ice. Chec" !ithyour laboratory forprotocols and !hetherplasma or serum is
needed. Be a!are that some
specimens need to besplit into t!o plasticvials before free5ingand sent fro5en on dryice. Chec" !ith yourlaboratory.
Post-test*
4plain significance oftest results andcounsel appropriatelyregarding presence ofinfection, recovery, andimmunity. Counselhealth care !or"ersand family regardingprotective andpreventive measures
necessary to avoidtransmission. Instructpatient to alert healthcare !or"ers andothers regarding theirhepatitis history insituations in !hicheposure to body fluidsand !astes may occur.
%ctober &,&'(8
Hepatitis Cantibody
#)nti-HC@$
his test detects thepresence of
antibodies to thevirus, indicating
eposure tohepatitis c virus.
(Manual ofLaboratory &
Diagnostic Tests by
Fischbach, 7thEdition, page ).3
C.%.@. '.&' '.3+on-reactive
+ormal
7/21/2019 Labs and Dx Vmmc Sepsis
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.A-ORATOR STUDIES
Com#lete-lood Count
his test is used to evaluate anemia, leu"emia, reaction to inflammation and infections, peripheral bloodcellular characters,2tate of hydration and dehydration.
Date Done Name of Test/Pocedue
Indication fo t!etest / Pocedue
Nomal"indings
ActualResults
Inte#etation andAnalysis
NusingRes#onsi$ilities
%ctober 8(,&'(8
Hemoglobin It is used todetermine the
concentration ofhemoglobin in !holeblood. It also serves
as an important
buffer in theetracellular fluid.(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page 7!
(8-(' g/= 0 g/= Inte#etation*
ecreased hemoglobinmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
Petest*
4plain test
procedure. 4plainthat slight discomfortmay be felt !hen s"in
is punctured.
)void stress if
possible becausealtered physiologicstatus influences andchanges normalhemogram values.
2elect hemogram
components orderedat regular intervals#eg, daily, every otherday$. hese shouldbe dra!n consistentlyat the same time ofday for reasons ofaccurate comparison6
+ovember ,&'(8
Hemoglobin It is used todetermine the
concentration ofhemoglobin in !holeblood. It also serves
as an importantbuffer in the
etracellular fluid.(Manual of
Laboratory &Diagnostic Tests by
(8-(' g/= g/= Inte#etation*
ecreased hemoglobinmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
7/21/2019 Labs and Dx Vmmc Sepsis
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7/21/2019 Labs and Dx Vmmc Sepsis
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Edition, page 7! needle.
:onitor the
puncture site for
oo5ing or hematomaformation. :aintainpressure dressingson the site ifnecessary. +otifyphysician of unusualproblems !ithbleeding.
;esume normal
activities and diet.
Bruising at the
puncture site is notuncommon. 2igns ofinflammation areunusual and shouldbe reported if theinflamed areaappears larger, if red
strea"s develop, or ifdrainage occurs.
+ovember ,&'(8
Hematocrit his test indirectlymeasures the ;BC
mass. he resultsare epressed asthe percentage byvolume of pac"ed
;BCs in !holeblood #PC@$. It is an
importantmeasurement in the
determination ofanemia or
polycythemia.(Manual ofLaboratory &
Diagnostic Tests byFischbach, 7th
Edition, page 7!
'.'-'. '.&3 Inte#etation*
ecreased hematocritmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
+ovember ,&'(8
Hematocrit his test indirectlymeasures the ;BCmass. he results
are epressed asthe percentage byvolume of pac"ed
;BCs in !holeblood #PC@$. It is an
importantmeasurement in the
determination ofanemia or
'.'-'. '.&( Inte#etation*
ecreased hematocrit
may be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
7/21/2019 Labs and Dx Vmmc Sepsis
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polycythemia.(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page 7!
%ctober 8(,
&'(8
Dhite blood cell It helps diagnosean
infection
orinflammatory
process6 it also maybe used to
determine thepresence of other
diseases that affectDBCs(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page !-
.' ('E0/=-
('.'('E0/=
&(.(0('E0/=
Inte#etation*
Increased !hite blood cellmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
+ovember ,
&'(8
Dhite blood cell It helps diagnoseaninfectionor
inflammatoryprocess6 it also maybe used to
determine thepresence of other
diseases that affectDBCs
(Manual ofLaboratory &
.' ('E0/=-
('.'('E0/=
(1.0('E0/=
Inte#etation*
Increased !hite blood cellmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
http://labtestsonline.org/glossary/infection/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/infection/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/infection/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/infection/7/21/2019 Labs and Dx Vmmc Sepsis
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Diagnostic Tests byFischbach, 7th
Edition, page !-
+ovember ,&'(8
Dhite blood cell It helps diagnoseaninfectionorinflammatory
process6 it also maybe used to
determine thepresence of other
diseases that affectDBCs
(Manual of
Laboratory &Diagnostic Tests byFischbach, 7th
Edition, page !-
.' ('E0/=-('.'
('E0/=
&'.(8('E0/= Inte#etation*
Increased !hite blood cellmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,
page .7
%ctober 8(,&'(8
=ymphocytes his test measuresthe number of
lymphocytes in theperipheral blood. It
operates in the
immune defenses ofthe body.(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page 6*
'.&' F '.' '.'0 Inte#etation*
ecreased lymphocytesmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
http://labtestsonline.org/glossary/infection/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/infection/http://labtestsonline.org/glossary/inflammation/http://labtestsonline.org/glossary/inflammation/7/21/2019 Labs and Dx Vmmc Sepsis
12/21
+ovember ,&'(&
=ymphocytes his test measuresthe number of
lymphocytes in theperipheral blood. It
operates in theimmune defenses ofthe body.
(Manual ofLaboratory &
Diagnostic Tests byFischbach, 7th
Edition, page 6*
'.&' F '.' '.( Inte#etation*
ecreased lymphocytesmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
+ovember ,
&'(&
=ymphocytes his test measures
the number oflymphocytes in theperipheral blood. It
operates in theimmune defenses of
the body.(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page 6*
'.&' F '.' '.(& Inte#etation*
ecreased lymphocytesmay be due to the clients
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page .7
%ctober 8(,&'(8
2egmentedneutrophils
his test determinesthe presence ofneutrophilia orneutropenia.(Manual of
Laboratory &Diagnostic Tests by
'.3'-'.1' '.08 Inte#etation*
Increased segmentersmay be due to
Analysis*
7/21/2019 Labs and Dx Vmmc Sepsis
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Fischbach, 7thEdition, page )
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page ..-
+ovember ,&'(&
2egmentedneutrophils
his test determinesthe presence ofneutrophilia orneutropenia.(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page )
'.3'-'.1' '.( Inte#etation*
Increased segmentersmay be due to
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page ..-
+ovember ,&'(&
2egmentedneutrophils
his test determinesthe presence ofneutrophilia orneutropenia.(Manual of
Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page )
'.3'-'.1' '.3 Inte#etation*
Increased segmentersmay be due to
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page ..-
7/21/2019 Labs and Dx Vmmc Sepsis
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Clinical
C!emisty
Blood c!emisty testing identifies many chemical blood constituents. It is often necessary to measure several blood
chemicals to establish a pattern of abnormalities. ) !ide range of tests can be grouped under the headings of en5ymes,
electrolytes, blood sugars, lipids, hormones, proteins, vitamins, minerals, and drug investigation.
Date done Name of Test/Pocedue
Indication fo t!eTest/ Pocedue
Nomal"indings
ActualResult/
"indings
Inte#etation andAnalysis
NusingRes#onsi$iliti
%ctober 8(,
&'(8
Potassium It evaluates changesin body potassium
levels and diagnosesacid-base and !ater
imbalances.(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7thEdition, page .6*
8.-.(
umol/=
.' umol/= NomalPe0test*
4plain purpos
procedure.
4ncouragerelaation.
Be a!are that
tourni9uet
application sho
be as brief as
possible !hendra!ing to pre
venous stasis
hemolysis.
4nsure that
supplements a
ta"en !ithin t
+ovember &,
&'(8
Potassium It evaluates changesin body potassium
levels and diagnosesacid-base and !ater
imbalances.(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7thEdition, page .6*
8.-.(
umol/=
8.& umol/= Inte#etation*
ecreased potassium may
be due to the clients
Analysis*
(Medical and "urgical
#ursing by $gnata%icius
and or'an, )th Edition,
page .7
+ovember ,
&'(8
Potassium It evaluates changesin body potassium
8.-.(
umol/=
&. umol/= Inte#etation*
7/21/2019 Labs and Dx Vmmc Sepsis
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levels and diagnosesacid-base and !ater
imbalances.(Manual of Laboratory
& Diagnostic Tests byFischbach, 7thEdition, page .6*
ecreased potassium may
be due to the clients
Analysis*
(Medical and "urgical
#ursing by $gnata%icius
and or'an, )th Edition,
page .7
hours before th
blood sample i
dra!n.
Post0Test*
Interpret test re
monitor chang
body potassiu
intervene as
appropriate.
Be a!are that
recogni5ing sig
and symptoms
hypo"alemia a
hyper"alemia i
important. :an
these originate
nervous and
muscular syste
and are usually
nonspecific an
similar.
+ovember ,
&'(8
Potassium It evaluates changesin body potassium
levels and diagnoses
acid-base and !aterimbalances.
(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7thEdition, page .6*
8.-.(
umol/=
8. umol/= Nomal
%ctober 8(,
&'(8
2odium It is used to determineelectrolytes, acid-
base balance, !ater
balance, !aterintoication, and
dehydration. (Manualof Laboratory &
Diagnostic Tests byFischbach, 7th
Edition, page .6!
(8-(3
m49/=
(3 m49/= Nomal
+ovember , 2odium It is used to determine (8-(3 (8 m49/= Nomal
7/21/2019 Labs and Dx Vmmc Sepsis
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&'(8 electrolytes, acid-base balance, !ater
balance, !aterintoication, and
dehydration. (Manualof Laboratory &Diagnostic Tests by
Fischbach, 7thEdition, page .6!
m49/=
;emember tha
potassium bloolevel rises '.3
#'.3 mmol/=$ fo
every '.( decr
in blood pH.%ctober 8(,
&'(&
Creatinine Itdiagnoses impairedrenal function. It is amore specific and
sensitive indicator of"idney disease than
B7+.
(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7thEdition, page .6*
1(-((
umol/=
8& umol/= Inte#etation*
Increased creatinine may
be due to
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page ..-
+ovember &,
&'(8
Creatinine Itdiagnoses impairedrenal function. It is amore specific and
sensitive indicator of"idney disease than
B7+.
(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7th
1(-((
umol/=
&18 umol/= Inte#etation*
Increased creatinine may
be due to
Analysis*
(Medical and "urgical#ursing by $gnata%icius
7/21/2019 Labs and Dx Vmmc Sepsis
17/21
Edition, page .6* and or'an, )th Edition,page ..-
+ovember ,
&'(8
Creatinine Itdiagnoses impaired
renal function. It is amore specific andsensitive indicator of"idney disease than
B7+.
(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7thEdition, page .6*
1(-((
umol/=
&&& umol/= Inte#etation*
Increased creatinine may
be due to
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page ..-
+ovember ,
&'(8
Creatinine It
diagnoses impairedrenal function. It is amore specific and
sensitive indicator of"idney disease than
B7+.
(Manual of Laboratory& Diagnostic Tests by
Fischbach, 7thEdition, page .6*
1(-((
umol/=
(0 umol/= Inte#etation*
Increased creatinine may
be due to
Analysis*
(Medical and "urgical#ursing by $gnata%icius
and or'an, )th Edition,page ..-
7/21/2019 Labs and Dx Vmmc Sepsis
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7/21/2019 Labs and Dx Vmmc Sepsis
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the ability of the lungsto oygenate the bloodand is used to assessthe effectiveness of
oygen therapy.(Manual of Laboratory& Diagnostic Tests byFischbach, 7th Edition,
page .37
Perform the
modified )llenAs testto assess collateral
circulation beforeperforming a radialpuncture, as follo!s*
4levate the
patientAs !rist !ith asmall pillo!, and as"the patient to etendthe fingers do!n!ard#this flees the !rist
and positions theradial artery closer tothe surface$.
:ean!hile,
ensure that all airbubbles in the bloodsample are epelledas 9uic"ly as
possible. )ir in thesample changes )B>values. Cap thesyringe and gentlyrotate to mi heparin!ith the blood.
=abel the sample
%ctober 8(,&'(8
Partialpressure of
carbon dioide#PC%&$
his test measures thepressure or tensioneerted by dissolved
C%&in the blood #('