LABORATORY ACTIVITY REPORT
FUNDAMENTAL OF BASIC SCIENCE II
CHAPTER 2: ENZYME ACTIVITY
1.Indah Permata Hasnul10100113125
2.Nadiya Afifah10100113126
3.Kemas Yasir10100113127
4.Ratu Rediani Utari10100113128
5.Ahda Febianti10100113129
6.Aprilia Puspasari10100113130
7.Yessi Cindy Agustin10100113131
8.Dinda Dwi Gusdinar10100113132
9.Fitria Nurhayati10100113133
10.Putri Maratu Sholiha10100113134
UNIVERSITAS ISLAM BANDUNG
FAKULTAS KEDOKTERAN
2013
DETERMINING FACTORS WHICH INFLUENCE AMYLASE ACTIVITYI. AIMTo
find out the factors that influence the activity of amylase
enzyme.II. PRINCIPLESaliva is produced by the submaxillary,
sublingual, and parotid glands, as well as by the mucouse membrane
and buccal glands of the mouth, throat, and esophagus. Saliva
contains about 99,5% of water. It also contains ptyalin or saliva
amylase, several proteins of which mucin, a glycoprotein, is the
most important, and a number of substances found in blood and urine
such as ammonia, amino acids, urea, uric acid, cholesterol calcium,
sodium, pottassium, magnesium, phospate, chloride, and
bicarbonate.Ptyalin or saliva amylase acts on strach, producing a
series of ill-defined products: amylodextrin, erythrodextrin,
achrodextrin and maltose. The activity of salivary amylase is
enchanced by the chloride ion. Salivary amylase acts as the
1,4-glucosidic linkages of amylase and amylopectin. The iodine
serves as an indicator. A dark blue color indicates the presence of
starch, the yellow iodine color means no starch remains, having
been hydrolized by the amylase.III. TOOLS AND MATERIALS- Saliva
(ptyalin enzyme)- small beaker
- Sodium chloride 0,1%- test tubes (6-10)
- Sodium chloride 1%- micropippete
- Sodium chloride 5%- stopwatch
- Aquadest
- Starch: Amylum (substract)
- Iodine 0,1 M (indicator)
IV. PROCEDURES
V. RESULTS
VI. CONCLUSION1) INFLUENCE OF COFACTOR (NaCl)2) INFLUENCE OF
TEMPERATURE3) INFLUENCE OF pH
MEASURING ASPARTAT AMINO TRANSFERASE (AST) ACTIVITYI. AIMTo
measure the activity level of AST enzyme.II. PRINCIPLEAspartate
Amino Trnsferase catalyses the transfer of Nitrogen from glutamate
to oxaloacetate according to the following equation:For the
quantitive AST determination the serum being tested is reacted in a
buffered III. MATERIALS
IV. PROCEDURES
V. CALCULATIONAST activity (IU/L) : (A/minute) X factorFactor:
340 nm 1746VI. RESULTSVII. CONCLUSION
