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Sperm function and sperm aneuploidies evaluated on
single cells selected with high magnification technique from
patients with severe testicular damage
Single sperm cells
Group A (n 100)
(nuclear vacuole)
Group B (n 100)
(normal sperm)
Mitochondria (%) 52.2+14.7* 13.3+4.9
Acridine Orange (%) 71.9+11.1* 5.3+3.0
TUNEL (%) 40.1+11.6* 9.3+4.8
Aneuploidies (%) 5.1+3.1 0.0
Garolla et al. High-power microscopy for selecting spermatozoa for ICSI by
physiological status. RBM Online, 2008
Aitken et al. Int J Androl 2009
Problems with DNA fragmentation:
What is to be measured and why?
Different methods
Standardization problems of the tests
Intra-individual variability
Normal values for fertility or normozoospermia?
Type of sample analysed
Small numbers
Subgrouping of infertile men and IVF/ICSI cycles
Identification of high-risk groups
Effect of abstinence time
Influence of sperm DNA damage
on IVF and ICSI Fertilization rate
Negative effect Larson, 2000
Tomlinson, 2001
Tomsu, 2002
Benchaib, 2003 (ICSI)
Larson, 2003
Henkel, 2004
Virro, 2004
Guerin, 2005
Borini, 2006 (ICSI)
Boe-Hansen, 2006 (IVF)
Avendano, 2009
Tarozzi, 2009 (ICSI)
No effect Seli, 2004
Bungum, 2004
Gandini, 2004
Huang, 2005
Zini, 2005
Borini, 2006 (IVF)
Boe-Hansen, 2006 (ICSI)
Bungum, 2007
Lin, 2008
Nucopoullos, 2008
Velez de la Calle, 2008
Tarozzi, 2009 (IVF)
Positive effect Payne, 2005
Pregnancy rate
Negative effect
Lopes, 1998
Host, 2000 (IVF)
Morris, 2002
Benchaib, 2003
Payne, 2005
Huang, 2005
Velez de la Calle, 2008
No effect
Tomlinson, 2001
Tomsu, 2002
Larson, 2003
Gandini, 2004 (ICSI)
Henkel, 2004
Virro, 2004
Guerin, 2005
Zini, 2005
Lin, 2008
Avendano, 2009
HA-ICSI significantly
improves embryo
quality and
development…but
not implantation rate
and pregnancy
New development Array CGH on single sperm