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r r-4 l_ a,-- l_ H l_ ).,..- l_ I-.- r_ )-.- l_. r--a L. ).-,-r, L --.- L Gonference Programs sth lnternational Conference on Plant Diversity 2015 (ICPD) 11th Gongress on lndonesian Society of Plant Taxonomy Theme: plant Diversity: A Global challenge Graha WidYatama U niversitas Jenderal Soedirman Thursday' Friday 20'21 August 2015 rlr TU LIPI

l Gonference Programs - 16.00 Murni Sapta Sari, Application of Project-Based Strategy on Algae Diversity Material as an Attempt to lmprove Biology Teacher Candidates Metacosnitive

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Gonference Programs

sth lnternational Conference on Plant Diversity 2015 (ICPD)

11th Gongress on lndonesian Society of Plant Taxonomy

Theme: plant Diversity: A Global challenge

Graha WidYatamaU niversitas Jenderal Soedirman

Thursday' Friday 20'21 August 2015

rlrTULIPI

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;e.+ "s -lnternational Conference On Plant Diverslty 2015

javanicum Miq, And 5. Aqueum ( Burm.F. ) . Alston) BasedOn Morpholosv Characteristic

14.30 - 14.4s Dewi Susan, Aohvllophorales Funei from Enesano lsland Graha Widvatama 0514.45 - 15.00 Bambang Heliyanto, Fatkhur Rochman, Abdurrahman,

Collection Of Sugarcane Genetic Resources ln Sarmi Paoua

Graha Wiciyatama 01

14.45 - 15.00 Dewi Komariah, Alex Hartana, Morphological Variation OfKabau (Arhidendron bubalinum) ln Sumatra

Graha Widyatama 05

15.00- 1s.15 Coffea BreakIime (WlB) Presenter of Session 2 Room

15.15 - 15.30 Sisunandar, Alkhikmah, Teguh Julianto, Capturing GeneticDiversity For Ex Situ Conservation Of Kopyor Coconut lnBanyumas Areas

Graha Widyatama 01.

15.L5 - 15.30 Retno kawuri, lsolation And Molecular ldentification With18s rDNA Leaf Base Rot Desease On Aloe Barbadensis lnBali

Graha Widyatama 05

15.30 - 15.45 Sarah Febriani, Characterization and Standarization ofCashew cultivars (Anacardium occidentale L)

Graha Widyatama 01

15.30 - 15.45 Kartini Kramadibrata, Diversity of Arbuscular Fungi inBatukau Nature Reserve and Mesehe Mountain. Bali

Graha Widyatama 05

15.4s - 16.00 Murni Sapta Sari, Application of Project-Based Strategy onAlgae Diversity Material as an Attempt to lmprove BiologyTeacher Candidates Metacosnitive Skills

Graha Widyatama 01

15.45 - 16.00 Pudji Widodo, Four New Subspecies Of SyzygiumSamarangense (Myrtaceae) From lndonesia

Graha Widyatama 05

15.00 - 16.1s Niken Kusumarini, Diversity of Cubeb in Java Graha Widyatama 0115.00 - 16.ls Endang Katikowati, Effect Of Combinations Various Plant

Spacing and Number of Seeds on Plant Growth and RiceYield Ciherang Varieties

Graha Widyatama 05

15.15 - 16.30 Tatik Chikmawati, Genetic diversity of wild rambutans(Nephelium) in Sanggau, West Kalimantan based on SSR

and ISSR markers

Graha Widyatama 01

15.15 - 15.30 Merry Antralina, The effect of Bokashi Dosage on theGrowth of Arabica Coffee Seedling (Coffea arabica L.)

Cultivar Andungsari l.

Graha Widyatama 05

16.30 - 15.45 Eko Sri Sulasmi, Spore Characters On Some Genus OfFilicales Allies

Graha Widyatama 01

16.30 - 16.45 Suratman,Analysis of Genetic Variability in Avocado (Persea

americana Mill.) Population ln Central Java Based onMorphological and Molecular Characters

Graha Widyatama 05

16.45 - 17.00 Mariana, Feliatra Felix, Sukendi, Syafruddin Nasution,Diversity of Mangrove Vegetation in Kuala tndragiri CoastalArea Riau Province - lndoneisa

Graha Widyatama 01

L6.45 - L7.OO Titien Ngatinem Praptosuwiryo, The Diversity of Ferns andFern Allies in Bogor Botanical Gardens

Graha Widyatama 05

17.00 - 17.15 Nurainas, Preliminary Study of the Genus Zingiber(Zingiberaceae) in Sumatra

Graha Widyatama 01

L7.00 * 77.15 Nunik Sri Ariyanti, The genus Bazzania of Mount Sibayak,North Sumatra

Graha WidVatama 05

17.t5 - 17.30 Wisnu Handoyo Ardi, Daniel Caspar Thomas, MarkHughes, Studies Begonia from Molucca lll: Reinstatementof two Begonia muricata svnonVms

Graha Widvatama 01

vi I enrsrrurnloN scHEDULE Fakultas Biologi I Universitas Jenderal Soedirman

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,**ii:f":, lnternational Conference On plant Diversity 2015

lsolation And Morecurar tdentification with 1gs rDNAOn Aloe Barbadensis tn Bali

Retno Kawuri

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Leaf Base Rot Desease

Laboratory Microbiorogy Facurty Mathematic and science UdayanaEm ail: [email protected]

ABSTRACT

Aloe vera plants (A.barbadensis Miller) has been planted in Bali since 2006 and spread out n fiveregencies, i'e' Buleleng, Karangasem, Bangli, Gianyar and Badung; invotving 13g farmers, withtotal area 170 hectare. Disease that h'ave oestroyeo Atoe vera L reif base rol witn the symptomof rotten base leaf' The objectives of this researih were to know the occurrence of pathogen inBali and to identify the cauCal agent that responsible for the leaf base rot disease on atoe. surveyon leaf base rot disease in Aloe vera piants was carrieJ out in 5 regencies in Bali, i.e.Karangasem, Buleteng,. Bangli, Gianyar and Badung. eainogen isolation on sample wasconducted until pure culture was obtained, and then wal inoculated on healthy Aloe vera plantsto confirm that the same symptom occurred- il tlre 1en irocrr eostulate test). The causal agentwas identified by molecular identification of 18s rDNA. Results showed that the leaf base rotdisease was distributed.in all five regencies in Bali wiih "r;;rg;incidence

was 13.43% and thecausal agent of the leaf base rot disease was Fusaium ir4"iZ*^.

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Acknowledgements

we have been supported by grant from Rector of Unsoed, who has given us permission ot usethis building for free' Dean and Vice Deans of Fakultas Biologi Unsoed are thanked for support.we also would like to thank Mr wisnu as the donatur. we would like to thank prof. Mien A.Rifai' and Prof' Dr' Dedy Darnaedi, who have trust us to be the committee of the 5thlnternational conference on Plant Diversity lcPD 2015 at Unsoed purwokerto. prof Ferry slikfrom the Herbarium of University Brunei Darussalam, Dr. Chandrakant Shalunke from Botanylndia and Dr campbell webb (Harvard University Herbaria), are acknowledged for their willingto be the keynote speakers. special thank we due to vijayasankar Raman phD (Univ. ofMississippi usA) for critically reviewing some papers. I wourd like to express my gratitude toProf' Dr Estri Laras Arumingtyas for assistance and advice. we are grateful to Dr Titi Rugayahand Mrs Yessi santika MSi who has coordinated this program from Bogor. we also would tiketo thank all participants who have come to Purwokerto for your presentation. , ,ro*i" .,the committee members of the lcPD 2015 who have worked hard for the success of thisconference' Finally, we hope that this conference can increase our awareness on theimportance of prant Diversity for our better future. Furthermore, we ;;;" ;;a by thisconference we can increase our collaboration amongst all participants and keynote speakers.Thank you.

Purwokerto,

August 20,2OlSCommittee

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