THIS 1'AI'....:1 :<oT TO ill!. CIT::n "(l1'1l0Ul' PHIUR HL;];'''; eITCl!: Tl'P';" Atr.i'JWR

•

International :ouneilfor the Exploration ofthe e"l.

On the use of saithe otolithe(Pollaehius virfns L )

in population an~ yels'

by A.Y. Storozbuk{ViaRol

C. '.197'5/ F; ,!

Demersal }'i a11(trorthern) olm"i tt-e

Otolith!! of fieh fOT:' " long time have been rather :'luecessfully

u ~d as 1~ nomie aien when differentiating individual popul~tions

cf po ly typle speeJ es (nollefeen, I9~;'j, irout", 1954; TemplcI!vql, ::;'1uir~ •

I"5G,rin~,I~67).

A5 working hypotr.e::is it. i8 as!;umcd that the aize ~nd the form

of otoliths 'ue genetic<>-lly fl:xed signs. Jo'urther a6 haa been oh,mn

in experiI:lental conditions the gro....th of otolithe to a great e.xten t ,

depende on ee010gieal f8~tors. Thua thc use of ot01iths in"popu­

l~tion lln?lysis will be more suecessful ~ith more strietly l~olat­

e p'Jpulatione ...nd w1th mor.e dletinetly different enviro'1n,ent"l "

eondit10n5 cf their habitat. This ~ropositicn answer5 the atl~~~t

-~de here to eo~aTe tne chBXPcteristieö of the growth of 5aith~

~tolit.h!'< c'üeul:<ted b· ue 'üth similar data obtalned by A.R.Lu-

cia. 19C' ~")r tre B'Ilthe 'Jf l.he :;orth-;,est Atlantic. Besid a, h ..

t'11. .. <'i 1 ej here C'3.n b'!. use.i I:: populat.ion :m~l1Y5tB of he i10rth-

}.'ateri;:,.] ("nd met..hods

1

an::! Janu'l.t"y 1974.. Complete biologie'.<l nniJ.lysi!l of f1::;11 \":ae made

directly In the ·see. und incluued .the determination of thc total

length, tot.ll.l we ight nnd' the wel&ht of, the spec irnen w ithout .viBce­

r,~ \"Iith anaccuracy of il.0 nll:1 a.nd :t 5 gr respeotlve1y.

In laboratory conditions the weight of otolithe was ascertalned

with an accuracy of ! 0.002 gr an.i tlle largest leneth of a whole

otolith vIHh~n'nccuracy of 0.2 mm.D~t~ on measurin~~the 1eft and the right otolithe were s~ml-

hr and·<i;~'thl~a.,lJ.a{~Si6we used thc !lerni-flUrn of, the weie,ht or e.. ., ....,~-~..,.

the lengnof both otol!tha.

The ~~tet"i~l was etatistic'l.lly processed under a program of

correlative-regress1ve analysis on computer "~insk-32".

lleoults and.discuBsion ..'ihe vurlabllity of thc e1zea~d wel&ht of flsh otoliths witLin

oi~e-eroups is partly accounted for by the Bex of the specimcns

('.:lacker,I97~) ,,:nd. in BOrne cas'es,c~nbe an obstacle in usint: ch~Lra ­

eteriatics or"otolith growt1~',wh~~ differentbtint; popul"tions. The

ßN\80n of the ye,,; n:.uy.'ale6 caus" the .varlllbllity of investiu... ted

lndlcts. In·.~his 'c6nn'ec~ion the first thing to da 1n our fl11'11~'.sisWq~ tcanswer the followine question: Does the relationship len~th~

-.el~ht 6r otolith-l~ngthoft~e 'specimen chnse ~ith the sei of\;., . '. ." . .

(;pcd~'dno Md the :sed.Bon) an! if ~o,t~ ',9hat e~tent)? ',:lth 'this ine'11",1 on th~ 1Il!>ter1al of1973 was,analys,,~ t1,e ratio between the

lengthof'the lJldthc, !'\.nd the weight of thc otol1ths both for males

l\n1 fem'\lee!l~pnr1\t~lY., ,

lt wall found thrl t 'betwt7en these signtl there io >l pos 1t 1ve Te-~ '., ,,~..,

t.~

2

(I)3d(Y) • 36.f'11O: :Jd(A) " I4.II7: ::'d(ll) = 0.205:

X m~~n =66.9; Sd(X me~n) =14.117; Y~~~J1.325;e: Gd(Yme~m).I37.74;

r-· t 0.964; 3d(r) " 0.OGC2; U • 130;

f~m~le6: Y " -247.9~ t 8.42x

Sd(Y) "37.192; Gd(A) " 14.152; 3d(3) == 0.203:

• (2) .

e",here

= 68.0; ::Jd(X~a.n) = I5.t:?O; Y!:1c:tn~ 325,5; Sd(Ynlean).137.~O

r •• 0.96~: Sd(r) " O.COf2: N " 138

Y;· weight ef etol1ths in IT.C r , '. ,.

X - length ef spec irr.cn in Cll:,

r - correlatbn faator,

3d- 3t'3-ndcord devi'ltion,'

öbs':lrvn.tions •.;

U - number ef

- ','Ohen cOlTTaring' equ'1.tions (I) und oe?) no re1i~le dlfferencc5\'1ere f::u:If

bi co~fflcient B (3tudent'8crlterton(t)~ et')uaite O:lO,:w{th

t.O.05 equ~} to 1.97). Dlfferenceo bi coeifici~nt:i~(t3tUd;=2.Ig).. \ " . - . '.

turne1 fe-out s1CnificIlnt for I'O,o5(tÖ•05 ==L:J7)a.n1,lt:&igniflcant

for- 1'0.01 (tO.OI ;. 2.60) •. Differencebe_t~een Y(1).:Um.Y(2)

(d (IO}- '(2) ) wasequal· to 17.4mgr,. i.e;- the ;"Jieht of otolHhs

in saltt .... m"tles 1s e;rM.ter (~pproltilll..:-t'31:t .~b/5:0.i:) th:m in fe--

; Clal~s or t>-te BOlIle lenGth (Fie;;".I). 3uch liifferences.1n·.weio;ht ef .-----\.e cll tM in -8I'ecies cf differ ... r.t &cxare ~~~oc~~~rV~d;~~ oth~r ­

species of f is~' (T,.!">p l .. r::An& 5quir"ls~I~56) and a.rebonne6ted \fit"

decr"'13 1nc cro'vth of ra1e::; due to thcir ·eu.rU"r-rr.~tur1tY.

Arter tl"ü on th~ r.:;t~-d"19 ·.obt'lin~d; in }'ebnxf.ry19H B_nd Au­

gust 197~ ""'::"'1 CAl,;ulat~"i ~~1.latrm: Y==A • .I>;( o} .lines ·or. regr!!s-

3

sl'Jnlellgth cf epeclm4n-length of otol1th~ In thls case .iutn for

:r.Ü(,;d f.aJ f~m'l.les were~ combi~d.

Y = 9.31 t O.1~4x (,3)

:3j('."} aO.R48; 3d(A} = '0.47+; ..>d(lJ} -0.(106;

:~:rean" 72.2; G;l(Xmean)' =1:!.84;' Ym"an.1S.?C;Sd(Yme(\n) =1.91

ra.O.000;' Sd(r)~0.OI0; U'.'10C;

A~lli'Ust: Y == 0.42 t O.I4~;~ (4)

3d(Y) '" 0.721; " 3d(A} = 0.430; 31(.3} =0.00e5;

~'1I~an ... 65.3; :3J (X;r;cun) ~I2.:;2; YiJleu.n .. 13.01; 8d(Ytle":n)ar.ü8 er=t 0.924; 3J(r) -0.018; ~ =84.

"1hen COI.lpl.rino; equ.'l.tions (3), anJ (4) no rp.lhole Jlffer~.,ce6

~ere foun1 either uy cQe!ficientB :(t"0.93~lth t o • 05a I.96) or by

coefficient A,(t=o.ec with.t O: 05:I.96) i.~. th~ lenßth~or otoliths

in ouith., of the sn.me, size'both'1n 'AU~uBt~ni ..'ebruary 'Il:l.S t,~", ~;ui~.

'1'hu~ ...,.hen difIerentir,.ting saithe populationsNith the help' r)f

cltHr.-.cteriotic5 cf' linea.r, g'~o.yth of otoll thB' d~p~"i1np' (Ihn.. ,.,,,gth. .,".,." ". .

ci tJ:f' q)"cl.uel1 i t, Is' derI:ri.bletb~t 3~X cf ,"pdciea, ahoulcl' be h\:en

hto aCCCU'1t. ,This ie o.l'Oc t~.l!t !~r the, ch·:..ractl!rt~tlcs cf t::.,::~!'

;:endence of weight ~ro~th"Of:~'~'olith~O~thelengthoI the.. . ". "

LIen as betwec'n the welght and theleni;th' of saithe otol1ths th~re

ia Tl high positive con!l~ctiol1:whlch'clm be clescrlbeJ a5 Y "AX3 ,

(on the Ill:I.terials of.January 1974) or in IOE;'l.rltllms 19Y'"lgA t nle;x,

(5)

::J.:l 19(Y) = ;}.OI32; 3d 19(A). ~O.0518; :3;1 19(B} ;: O.021I;

19 Xm 3.n. 2.455; Sd 19(~e~~l~0.08I: Sd, ISYlneaLl~1.25~;

Sd.lg (Ymean ) =0.0287; ,

r .:: tO.890; 3d(r},.::O.02?;>N " 6I.

4

-' ~

....•. ...,;

j30

20::J~0g 10

060' 80

'.-"

1"1ah. leDgtilL (oa)

100

•

"

0,7

'""

g 0,3og.

0,1

o

•

So'leDgtlh, (oa)

100 co

',Ye have cOlnpared other authors' data on otolith length in

a ingle s ize sai the of t he North-:-res t Atlantic wit h our reaults

for the aaithe of the North Sea. In thlB caBe we could not take

into account the aex of specimena aB Lucio's work cites comoined

<!:ata botb on sA.ithe males and femalea caught In August.

The compari~0n 0: the two lines of regre33ions (Fig.2) haa

sho·....n that otolith '-engh of sl'dthe 01' the ~:rort.h Sea 15 higher

than in snithe of tile Uorth-'./est Atlantiv. This corre3ponda to

~ifferences between the two populations in gro~th rate,which in

ontogenesis alows down earlier'ln tfe saithe 01' the North 3ea

res\llting f'rom earlier sexual maturity (Svetovidov,I94'7).

ifferences in the rate, of growth in indiVidual eaHhe 0­

pub.!. iOllS of the lIorth-east Atlantic marked hy Cieglewicz,Dr:lg'lnik

(JSlI."!) 6ue.~e::lt tbe possibtli ty to use tbe data above and below

on (ne Horth Be', salthe in population analysis of this polytypic

ßpecies.

11 tbe materials collected in April 1973 the rel~tionship of the

"u~Jl ~eight pr the 8aithe and the welght of otolithB ~an be given

as (6)

Sd(Y) =43.3 5; ",d(.A). 4.856; Sd(.I3) .0.0014;

Xmean ~2908; 3d(Xmenn): 1832; Ymean • 330.1 Sd(Ymean ) .d40.79

_ r :tO.952; 3d(r) =0.0056; N =281,

~he relatlonship between the weigbt of the saithe .ithout vis-

cera and the weight of otoliths can be g1ven aa

Y a 117.79 t 0.Oe3x (7)

Sd(Y) = 40.639; Sd(A) :4.529; ",d(n) .. 0.0015;

Xmean= 2550; Sd(Xmean) :1619; Ymean" 330.1; Sd(Ymean ) • 140.79;

r ~t 0.957; Sd(r) :0.0049; N .~81,

5

(n)

Ihe re1atlonohip betweeo the 1ength of the sait~· ~nd the

-eight of oto11tho CCln be given os

y : -253.30 • 8.5Clx

3d(y) =43.209; ~d(A) : IO.8~6; Gd(B) = 0.1534;

Xme~~ =68.7; Sd(Äme~n) =10.18; Yme n=334.7 ; Sd(Ymean).145.1

r ~ .0.954; Sd(r) =0.015; N = 3n4

19 Y = -0.5577 • 1.669 19 X

or

(9 )

Jd 19 (Y) c 0.0451; Sd 19(A) .O.C~68; Gd 19 (B) = O.O?56;

1 X mean =1.825; Gd 19 (Xme~n) = .1011; Lg Ymean

= 2.488;

3d 19(Ymean): 0.1746;

r = • 0.966; 'Sd(r) =O.003!; N= 304.

Reftlrence

I. blacker R.W.,I974. Reeent Bdvancee in otolith studies

In "Sea Fiehe't"ies escarch" edited by

l!'.R. Harden Jones.London p.67-90.

2. 3vetlovidov A.N.,I948. Hyby.~reskoobraznye,v.IX

3. Cieglewiez W.,Drag n±k B.I969 Char~terystikawzrostu Czar­

niaka (P.virene L.) Z ~orzu Nor~eskiego

~ i Morzu Fol.Praee ~Qrsk'ego Inet.Rybak

t.I5. Ser.A 6.133-152.

4. Lueio A.H.,1967 Datos biometrieos y biologicoe sobre el

Carbonero (r.virens L.) deI sur de

Terranova. ßoletin deI Inst.hapanol da

Oceanografla. No133. 23 p.

5. ~i~a U.V. 1967 Issledovanie zavisimosti mezhdu vesom otolita

(s~gittQ) 1 dlinoy osobi v populJatsiJakh

treski Barenteeva i .dclogo morey. "Biolog.

l,auki" No9 pa 26-:H.

6. 1\011efsen 0.,1933. The otolith of the cod .b'isk.3kr.Ser h ...vunders

4: 13 14 p.

11.. Templellllin '!T. and SquIres H.J,I95e. Relatiooship of otolith od

~eights iu the haddock clanogrammus aegle-e frous (L) to the rate of growth of th e fish.

],'ish Hes ild C..na.da,1.3 p 467 -487.

8. frout G.C •• 195~. otolith growth of the Barents Se eod.l~pp.

P.-V. Heun.Gons iot ßxp. ~er.NoI36 p.89-I02.

7

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