KS + Ima + Zvad % of positive cells (ANV+) Cells positive for SA--gal (%) Cells positive for SA--gal (%) % of positive cells (ANV+) Supplementary Figure 1 n = 8 A-B- C-D- KS + Ima + Zvad K562 K562 + Ima K562 + Ima + Zvad Supplementary Figure 1E BD Drullion et al AC Hsp60 LC3B KSKS shATG7 Ima BafA Hsp60 LC3B Hsp60 LC3B KS KS shATG7 HBSS - BafA1 06h 12h9h 12h 06h 12h9h 12h HBSS + BafA1 Supplementary Figure 2 A- B- KSKS shATG7 Control Ima Supplementary Figure 2C K562 sh ATG7 K562 sh ATG7 + Ima K562 sh ATG7 + Ima + Zvad A B C D Supplementary Figure 3A Drullion et al Ima Tetra--++ sh casp3sh casp9 Casp3 Casp9 p21 p27 hsp60 Cleaved Casp3 % of positive cells (ANV+) Supplementary Figure 4 n = 3 A- B- Cells positive for SA- -Gal (%) Supplementary Figure 4 n = 3 C- sh-scr sh-Bcn control sh-ATG ATG5 Hsp 60 Beclin 1 Hsp 60 Supplementary Figure 5 A- Ima Zvad Mean fluorescence intensity n = 3 *p