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Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Page 1: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

Know how. Know now.

SICNA 29 Aug 2013 – Lubbock TX

Improving the use of sorghum distillers grains in beef cattle diets

Jim MacDonald, PhD, PAS

Page 2: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

Know how. Know now.

Status of sorghum distillers grains in beef cattle scientific literature

Owens, 2008

Page 3: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Does Grain Type Influences WDGS?

100% Corn 100% Sorghum

Page 4: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Nutrient profile of sorghum and corn

Item Corn Sorghum

NDF 10.1 8.7

ADF 3.7 5.2

Lignin 1.2 1.3

Hemicellulose 6.4 3.5

Hemicellulose % of NDF

63.2 40.0

Source: Dairy One Feed Library

Page 5: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Does grain type influences nutrient profile?

Item Corn WDGS

Sorghum WDGS

Blended WDGS

CP 26.8 40.0 30.4

Fat 11.0 7.0 10.1

NDF 23.3 43.9 36.0

S 0.74 0.45 0.61

Solubles may have varied considerably

Page 6: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effect of Corn Processing and NDF Content on the Digestibility

of WDGS

DRC vs. SFC (P = 0.73)CWDGS vs. SWDGS (P < 0.01)

Interaction (P = 0.60)Assumes kp = 0.05/hr

Ru

min

al D

M D

ige

sti

blit

y, %

(Lewis et al, 2009. J. Anim. Sci. 87E-Suppl. 3:40 (Abstr.)).

Page 7: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Relationship of NDF and Energy in WDGS

(JC MacDonald, unpublished).

Page 8: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Strategies to Improve Fiber Digestibility

Page 9: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Titration of limestone on the pH of wet distillers grains

Page 10: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effect of buffer and enzyme on fiber content and pH

  No enzyme Added enzyme   -P-Value-

Item No Buffer Buffer No Buffer Buffer SE Buffer Enzyme Interaction

Initial pH 3.68c 5.78a 3.63d 5.34b 0.01 <0.01 <0.01 <0.01Final pH 3.68c 5.17a 3.55d 4.46b 0.01 <0.01 <0.01 <0.01Final DM, % 33.1g 34.0f 33.3g 34.7e 0.1 <0.01 <0.01 0.05

Final NDF, % 40.6 40.7 37.9 37.2 1.61 0.83 0.05 0.82

Final ADF, % 16.9 17.1 16.6 17.4 0.82 0.51 0.93 0.71

Means within row with different superscripts differ (P < 0.05).

Page 11: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effect of buffer and enzyme on digestibility parameters

  No enzyme Added enzyme   -P-Value-

Item1 No Buffer Buffer No Buffer Buffer SE Buffer Enzyme Interaction

IVTD, % 79.7 81.2 81.3 82.5 0.9 0.14 0.10 0.85

NDFD, % 50.0 54.2 50.7 53.1 2.3 0.14 0.91 0.67

ADFD, % 46.9 51.7 56.4 59.3 2.5 0.12 <0.01 0.69

INDF, % 20.3 18.8 18.7 17.5 0.8 0.14 0.10 0.85

IADF, % 8.98 8.26 7.24 7.10 0.4 0.28 <0.01 0.47

IHEMI, % 11.4 10.6 11.5 10.4 0.5 0.07 0.93 0.77

Page 12: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effect of buffered enzymes on rumen digestibility

Item UNT ENZ BUFFERED NaOH NaOH+ENZ SE P-value

pH 3.27 3.25 4.71 4.96 4.46 - -Dry Matter              

a (%) 47.7d 54.5b 60.7a 49.8c 54.3b 0.5 <0.01b (%) 45.2 40.9 38.0 40.5 39.3 2.6 0.17c (%/hr) 2.76ab 1.77b 1.89b 3.09a 2.37ab 0.5 0.06ERD (%) 63.3d 65.1bc 69.6a 64.0cd 66.1b 1.1 <0.01Extent (%) 89.1 87.9 88.5 86.4 88.2 1.5 0.45

Page 13: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effect of buffered enzyme on rumen ADF digestibilityItem UNT ENZ BUFFERED SEM P-value

ADF, % 25.1 27.3 20.5 - -a (%) 31.7c 44.1a 40.1b 0.2 <0.01b (%) 61.1 44.9 53.3 6.7 0.13c (%) 2.64 2.62 2.28 0.6 0.83ERD (%) 51.3b 59.3a 54.4b 1.5 0.01Extent (%) 85.1a 85.4a 79.7b 4.6 0.07

Indigestible ADF

12.2 11.1 9.3 -  

Page 14: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Summary of Laboratory Work

• Enzymes solubilize fiber• Remaining fiber has similar digestibility• Greater overall digestion

Page 15: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Animal performance and digestion

Page 16: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Nutrient profile of WDGS fed to growing steers

Item Control1 Enzyme Buffered Enzyme

CP 35.1 32.1 32.6

NDF 35.7 24.7 22.9

ADF 21.2 15.6 15.0

Crude fat 11.4 11.4 11.4

Ca 0.11 0.11 3.99

P 0.87 0.81 0.77

K 1.07 1.02 0.96

S 0.58 0.54 0.53

Page 17: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effect of buffered enzyme on performance of growing steers

Item Control Enzyme Buffered Enzyme

SEM P-value

Initial BW, lb

584 584 584 1 0.99

Ending BW, lb

868 873 854 9 0.32

ADG, lb 3.39 3.44 3.22 0.11 0.32

DMI, lb2 19.6ab 20.3a 18.5b 0.5 0.04

G:F 0.173 0.169 0.174 0.004 0.66

F:G 5.78 5.92 5.75 - -

Page 18: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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In Vivo Digestibility Estimates

Item Control Enzyme Buffered Enzyme

SEM P-value

OM Dig 76.0 73.8 76.0 1 0.23

NDF Dig 54.7 55.0 56.6 1.1 0.45

Page 19: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Summary of buffered enzyme work

• Buffered enzymes showed promise in vitro• Animal trial do not support improved

performance• Animal performance trial• In vivo digestion trial

Page 20: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Effects of NaOH on in vitro digestibility

% NaOH

% D

iges

tibili

ty

15% increase in fiber digestibility

(JC MacDonald, unpublished).

Page 21: Know how. Know now. SICNA 29 Aug 2013 – Lubbock TX Improving the use of sorghum distillers grains in beef cattle diets Jim MacDonald, PhD, PAS

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Acknowledgements

Funding• United Sorghum Checkoff• Texas Corn Producers

Board• Biozyme Incorporated• USDA-ARS• Texas State Distillers

Grains Initiative

Research Team• Dr. Ted McCollum• Dr. N. Andy Cole• Dr. Jhones Sarturi• Dr. Christian Ponce• Jodi Patterson• Beverly Clark• Casey Brauer• Stacey Butler• Robby Bondurant