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Kenneth Vincent Mills College of the Holy Cross Phone: 1-508-793-3380 Department of Chemistry FAX: 1-508-793-3530 1 College Street E-mail: kmills@holycross.edu Worcester, MA 01610 Employment 2001-present College of the Holy Cross, Department of Chemistry, Worcester, MA

•Professor, 2014- •Department Chair, 2010 - 2014 •Associate Professor, 2007-2014 •Assistant Professor, 2001-2007

Education 1996-2001 Harvard University, Cambridge, MA

•Department of Chemistry and Chemical Biology •A.M. 1998, Ph.D. 2001, Advisor: Prof. Henry Paulus

1992-1996 Loyola College in Maryland, Baltimore, MD •B.S., summa cum laude, 1996, Chemistry major, philosophy minor Research Interests Protein splicing, mechanistic enzymology, protein chemistry, guided-inquiry undergraduate lab development Research Experience 2001-present College of the Holy Cross, Department of Chemistry, Worcester, MA •Direct undergraduate researchers in the study of the mechanism of

hedgehog autoprocessing and protein splicing. 1996-2001 Harvard University, Department of Chemistry and Chemical Biology,

Cambridge, MA •Studied mechanism of protein splicing and developed an in vitro trans-splicing protein ligation system using inteins from RecA protein of Mycobacterium tuberculosis and DnaE protein of Synechocystis species PCC6803.

1995 Summer Research Institute for Applied Science, Lawrence Livermore

National Laboratory, Livermore, CA •Studied peptide models of prion protein folding.

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1991-1996 Medical Research Institute for Chemical Defense, Aberdeen Proving Grounds, Edgewood Area, MD •Studied and designed active site mutants of human butyrylcholinesterase that demonstrated hydrolysis of organophosphate inhibitors.

Teaching Experience 2001-present College of the Holy Cross, Department of Chemistry •Montserrat-Drug Design, Action and Abuse (Natural World cluster, Fall 2012 and 2013) •Montserrat-Biotechnology and Ethics (Natural World cluster, Spring 2013 and 2014)

•Atoms and Molecules, Chemistry 101/181 Lecture and Lab (Fall 2001, 2002, 2004, 2005, 2006, 2008) •Introduction to Equilibrium and Reactivity/Equilibrium and Reactivity, Chemistry 231 Lecture and Lab (Spring 2002-2007, 2009-12) •Biochemistry, Chemistry 301, Fall 2008, 2010-13 •Bioorganic Chemistry, Chemistry 310 (Spring 2003, 2006) •Advanced Biochemistry, Chemistry 363 (Spring 2004) •Biochemistry II Laboratory, Biology 304 (Spring 2002)

2000-2001 Harvard University, Division of Continuing Education •Head teaching fellow for General Chemistry course. 1997-2000 Harvard University, Division of Continuing Education

•Teaching fellow for General Chemistry

1996-1997 Harvard University, Department of Chemistry and Chemical Biology •Teaching fellow for Organic Chemistry

External Grants 2015-2018 National Science Foundation, RUI Grant, $748,268 RUI:TheCatalysisofProteinSplicingandtheStructureandStability ofThermophilicInteins:AnIntegratedResearchandEducation Program (http://news.holycross.edu/blog/2015/10/15/with-1-5m-in-nsf-funding-holy-cross-professor-makes-student-research-opportunities-a-priority/) 2013-2016 National Science Foundation, RUI Grant, $567,115 RUI: Intein Structure and Catalysis of Protein Splicing and Hedgehog Autoprocessing: An Integrated Research and Education Program 2012 National Science Foundation, REU supplement to RUI grant, $6,500 2010-2013 National Science Foundation, RUI Grant, $465,000

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RUI: Catalysis and Coordination of the Steps of Protein Splicing by Non- canonical Inteins: An Integrated Research and Education Program 2009-2016 Henry Dreyfus Teacher Scholar Award, $60,000 2010 National Institutes of Health, Subaward, $25,000 Subaward to collaborator Chunyu Wang’s NIH ARRA supplement (Rensselaer Polytechnic Institute) 2005-2010 National Science Foundation, CAREER grant, $794,482

“CAREER: Alternative Mechanisms of HINT domain autoprocessing: An integrated undergraduate research and education program”

2009 American Society for Biochemistry and Molecular Biology, Undergraduate Outreach Award, $250

2003-2005 National Science Foundation, Major Research Instrumentation, $255,502

“Acquisition of Protein Purification and Analysis Equipment to Enhance Biochemical Research and Education at the College of the Holy Cross”

2003-2005 Research Corporation, Cottrell College Science Award, $58,028

“The Mechanism and Molecular Evolution of Hedgehog Proteins and Related Auto-Processing Domains”

2002-2004 Petroleum Research Fund (American Chemical Society), $35,000 Type GB, “The Mechanism of Protein Splicing and the Molecular Evolution of Inteins.” 1997-2001 Howard Hughes Medical Institute Predoctoral Fellowship 1995-1996 Barry M. Goldwater Fellowship Internal Grants 2013 Hewlett Mellon Presidential Discretionary Fund Summer Workshop on Emerging Challenges in STEM Education 2012 Hewlett Mellon Presidential Discretionary Fund, Chemistry Chemistry Department Review: Reflection and Response 2009 Hewlett Mellon Presidential Discretionary Fund, Biochemistry

Concentration Assessment: Refinement and Implementation 2008 Hewlett Mellon Presidential Discretionary Fund, Biochemistry

Concentration Assessment: Mission, Goals and Student Learning Outcomes

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2003 Research and Publication Award 2002 Charles and Rosanna Batchelor (Ford) Foundation Summer Fellowship (declined to accept external funding) Awards/Fellowships 2015 Cottrell Scholar, Research Corporation 2013 Mary Louise Marfuggi Award – Outstanding Scholarly Accomplishments 2009 Henry Dreyfus Teacher Scholar Award, Dreyfus Foundation 2009 Undergraduate Outreach Award, American Society for Biochemistry and

Molecular Biology 2002 Charles and Rosanna Batchelor (Ford) Foundation Faculty Fellowship 1997-2001 Howard Hughes Medical Institute Predoctoral Fellowship 1996, 1997 National Science Foundation Fellowship honorable mention 1996 American Institute of Chemists, D.C. chapter, chemistry award 1994-1996 Barry M. Goldwater Scholar 1994-1996 Datatel Scholar 1996 Award for highest overall GPA, Loyola College in Maryland 1996 Chemistry Medal, Loyola College in Maryland 1995 Phi Beta Kappa, Alpha Sigma Nu (Jesuit Honor Society) 1992-1996 Dean's List, Loyola College in Maryland, all semesters 1992-1996 Marion Burk Knott Scholarship, Maryland Distinguished Scholar 1992-1993 National Merit Scholar, Byrd Scholar Publications (Undergraduate co-authors indicated by asterisk) Holy Cross publications: 30. Mills, K.V. (2015) Biochemistry in an undergraduate writing-intensive first-year program: Seminar courses in drugs and bioethics. Biochemistry and Molecular Biology Education 43, 263-272. 29. Kulevich, S.E., Herrick, R.S., and Mills, K.V. (2014) A Discovery Chemistry Experiment on Buffers. J. Chem. Educ. 91, 1207-1211. 28. Mills, K.V., Johnson, M.A., and Perler, F.B. (2014) Protein splicing: How inteins escape from precursor proteins. Journal of Biological Chemistry, 289, 14498-14505. 27. Topilina, N.I. and Mills, K.V. (2014) Recent Advances in in vivo applications of intein-mediated protein splicing. Mobile DNA 5, 5.

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26. *Nicastri, M.C., *Xega, K., Li, L., Xie, J., Wang, C., Linhardt, R.J., Reitter, J.N., and Mills, K.V. (2013) Internal Disulfide Bond Acts as a Switch for Intein Activity. Biochemistry 52, 5920-5927. 25. Mills, K.V. (2013) Self-Splicing Proteins. In: Handbook of Proteolytic Enzymes, Third Edition, Vol. 1., ed. N. Rawlings and G. Salvesen, 315-321. 24. Chen, W., Li, L., Du, Z., Liu, J., Reitter, J.N., Mills, K.V., Linhardt, R.J. and Wang, C. (2012) Intramolecular Disulfide Bond between Catalytic Cysteines in an Intein Precursor. J. Am. Chem. Soc.,134, 2500-2503. 23. Du, Z., Liu, J., Albracht, C.D., Hsu, A., Chen, W., *Marieni, M.D., *Colelli, K.M., *Williams, J.E., Reitter, J.N., Mills, K.V. and Wang, C. (2011) Structural and Mutational Studies of a Hyperthermophilic Intein from DNA Polymerase II of Pyrococcus abyssi. J. Biol. Chem. 286, 38638-38648. (Selected for Nov. 4, 2011 cover.) 22. Liu, J., Du, Z., Albracht, C.D., Naidu, R.O., Mills, K.V. and Wang, C. (2011) 1H, 13C, and 15N NMR assignments of the Pyrococcus abyssi DNA polymerase II intein. Biomol. NMR Assign. 5, 233-235. 21. Reitter, J.N. and Mills, K.V. (2011) Canonical protein splicing of a class one intein that has a class three non-canonical sequence motif. J. Bacteriol. 194, 994-997. 20. *O’Brien, K.M., *Schufreider, A.K., *McGill, M.A., *O’Brien, K.M., Reitter, J.N. and Mills, K.V. (2010) Mechanism of protein splicing of the Pyrococcus abyssi lon protease intein. Biochem. Biophys. Res. Commun. 403, 457-461. 19. *Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N., and Mills, K.V. (2009) Protein splicing of the three Pyrococcus abyssi ribonucleotide reductase inteins. Biochem. Biophys. Res. Commun. 387, 153-157. 18. Mills, K.V., Herrick, R.S., Ditzler, M.A., Nestor, L.P., Shafer, H., and Guilmette, L.W. (2008) An Introduction to Electrochemistry: A Two-week Discovery Chemistry Experiment. J. Chem. Educ. 85, 1116-1119. 17. Herrick, R.S., Mills, K.V., and Nestor, L.P. (2008) Data Pooling in a Chemical Kinetics Experiment: The Aquation of a Series of Cobalt (III) Complexes. J. Chem. Educ. 85, 1120-1122. 16. Mills, K.V. and Guilmette, L.W. (2007) Thermochemical Analysis of Neutralization Reactions: An Introductory Discovery Experiment. J. Chem. Educ. 84, 326-328. 15. Bellin, R.M. and Mills, K.V. (2007) The Holy Cross Biochemistry Concentration: An Integrated Four-Year Program to Develop Undergraduate Research Scholars. In Developing and Sustaining a Research-Supportive Curriculum, ed K.K. Karukstis and T.E. Elgren, Council on Undergraduate Research.

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14. Mills, K.V., Connor, K.R., *Dorval, D.M., and *Lewandowski, K.T. (2006) Protein Purification via Temperature-Dependent, Intein-Mediated Cleavage from an Immobilized Metal Affinity Resin. Anal. Biochem. 356, 86-93. 13. Mills, K.V., *Dorval, D.M., *Lewandowski, K.T. (2005) Kinetic analysis of the individual steps of protein splicing for the Pyrococcus abyssi PolII intein. J. Biol. Chem 280, 2714-2720. 12. Mills, K.V., *Gareau, J.R., and *Garcia, A.M. (2005) Low-cost modification to the Copacabana method for spreading transformation mixtures. BioTechniques 39, 188. 11. Mills, K.V., Perler, F.B. (2005) The Mechanism of Intein-Based Protein Splicing: Variations on a Theme. Protein & Peptide Letters, 12, 751-755. 10. Mills, K.V. (2005) Protein Splicing. In Encyclopedia of Molecular Cell Biology and Molecular Medicine, Second Edition, ed, RA Meyers, 241-265, Weinheim: Wiley-VCH. 9. Mills, K.V., Paulus, H. (2005) Biochemical Mechanisms of Intein-Mediated Protein Splicing. In Nucleic Acids and Molecular Biology 16 (Homing Endonucleases and Inteins), ed. M. Belfort, 231-255, Berlin: Springer Verlag. 8. Mills, K.V., *Manning, J.S., *Garcia, A.M., *Wuerdeman, L.A. (2004) Protein splicing of a Pyrococcus abyssi intein with a C-terminal glutamine. J. Biol. Chem. 279, 20685-20691. 7. Mills, K.V. (2004) Self-Splicing Proteins. In: Handbook of Proteolytic Enzymes, Second Edition, Vol. 2., ed. AJ Barrett, 2136-2140, London: Academic Press, Elsevier Science. 6. Mills, KV. (2003) Post-Translational Control. In: Genetics, Vol. 3., ed. R Robinson, 178-182, New York: Macmillan Reference USA. Pre-Holy Cross Publications: 5. Mills, K.V., Paulus, H. (2001) Reversible Inhibition of Protein Splicing by Zinc Ion. J. Biol. Chem. 276, 10832-10838. 4. Lew, B.M., Mills, K.V., Paulus, H. (1999) Characteristics of Protein Splicing in trans Mediated by a Semisynthetic Split Intein. Biopolymers (Peptide Science) 51, 355-362. 3. Lew, B.M., Mills, K.V., Paulus, H. (1998) Protein Splicing in Vitro with a Semisynthetic Two-Component Minimal Intein. J. Biol. Chem.273, 15887-15890.

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2. Mills, K.V., Lew, B.M., Jiang, Sq, Paulus, H. (1998) Protein splicing in trans by purified N- and C- terminal fragments of the Mycobacterium tuberculosis RecA intein. Proceedings of the National Academy of Sciences, USA 95, 3543-3548. 1. Broomfield, C.A., Mills, K.V., Meier, B.M., Lockridge, O.A., and Millard, C.B. (1998) Reaction of human butyrylcholinesterase (BchE) H117 enzymes with carbamates. In: Structure and Function of Cholinesterases and Related Proteins, 223-226. Abstracts (Recent, first author presenter) Jaramillo, M., Williams, J., Reitter, J. and Mills, K. (2015) Structure and Activity of Inteins from Pyrococcus abyssi and Pyrococcus horikoshii. FASEB J. 29, 722.4. Yakely, A., Bonano, A., Jaramillo, M., Williams, J., Reitter, J., and Mills, K. (2015) Relating Intein Flexibility to the Temperature Dependence of Activity. FASEB J. 29, 722.3. Siegart, N., Colelli, K., Reitter, J. and Mills, K. (2015) Protein Splicing of a Temperature-Dependent Intein from an Extreme Thermophile. FASEB J. 29, 573.12. (Best Undergraduate Poster – Proteins and Enzymes: www.asbmb.org/News.aspx?id=63127) Giaccone, Z., Reitter, J., Steeves, A., Kulik, H. and Mills, K. (2015) Peptide Bond Cleavage through Asparagine Cyclization. FASEB J. 29, 722.9. Koulopoulos, M., Giaccone, Z., Reitter, J., and Mills, K. (2015) A Biophysical Analaysis of Asp Cyclization Coupled to Peptide Bond Cleavage. FASEB J. 29, 722.2 Mills, K. (2015) Integrating Biochemistry into a First-year Undergraduate Rhetoric-Intensive Seminar Program. FASEB J. 29, 559.1. Mills, K., Reitter, J., Nicastri, M., Colleli, K., and Williams, J. (2015) Protein splicing: Regulation by Temperature and Oxidation State of Non-Canonical Inteins, Biophysical Journal 108 (2-Sup.1), 30a 144-Plat. *Jaramillo, M.V., *Nicastri, M.C., Reitter, J.N. and Mills, K.V. (2014) Protein splicing of an intein from the extreme halophile Halobacterium salinarum. FASEB J. 28, 768.9. *Haley, D.S., *Siegart, N.M., Reitter, J.N. and Mills, K.V. (2014) Structural analysis of human carbonic anhydrase II and a thermophilic intein. FASEB J. 28, 768.3. *Cahn, S.A.,Reitter, J.N. and Mills, K.V. (2014) Directed evolution toward inteins with alternative protein splicing mechanisms. FASEB J. 28, 566.2.

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*Giaccone, Z.T., *Urbanski, L.M., *Siegart, N.M., Reitter, J.N. and Mills, K.V. (2014) Peptide bond cleavage through cyclization of asparagine. FASEB J. 28, 768.4. *Siegart, N.M., *Colelli, K.M., *Williams, J.E., Reitter, J.N. and Mills, K.V. (2014) Protein splicing of a temperature-dependent intein from an extreme thermophile. FASEB J. 28, 768.10. Mills, K.V., Reitter, J.N., *Nicastri, M.C., *Williams, J.E., and *Colelli, K.M (2014) The stops and starts of protein splicing: regulation and mechanism of non-canonical inteins. FASEB J. 28, 584.1. *Siegart, N.M., *Urbanski, L.M., *Karanja, K., Reitter, J.N. and Mills, K.V. (2013) Protein Splicing of Non-canonical Inteins from Cyanobacteria Protein Science 22-Sup1, 234. Mills, Kenneth V., Reitter, J.N., *Nicastri, M.C., *Williams, J.E., *Colelli, K.M., and *Marieni, M.D. (2013) The Structure and Regulation of Non-Canonical Inteins. Protein Science 22-Sup1, 93. *Williams, J.E., Reitter, J.N. and Mills, K.V. (2013) Structural analysis of an intein from an extreme thermophile. FASEB J. 27, 1005.1. (Honorable Mention – Best Undergraduate Poster) *Colelli, K.M., *Pusztay, J.M., Reitter, J.N. and Mills, K.V. (2013) The role of an extended beta-sheet in stabilizing the structure of a thermophilic intein. FASEB J. 27, 1005.2. (Honorable Mention – Best Undergraduate Poster) *Nicastri, M.C., *Xega, K., Reitter, J.N., and Mills, K.V. (2013) Conditional protein splicing via disulfide bond formation. FASEB J. 27, 789.3. *Siegart, N.M., *Urbanski, L.M., *Karanja, K., *Colelli, K.M., Reitter, J.N. and Mills, K.V. (2013) Protein splicing of inteins from Synechococcus sp. PCC7002 and Trichodesmium erythraeum. FASEB J., 27, 789.7. *Cahn, S.A., *Savidis, G., *Drago, M.J., Reitter, J.N. and Mills, K.V. (2013) Expression and auto-processing of hedgehog-like proteins from Brugia malayi and Cryptosporidium. FASEB J. 27, 789.4. *Pusztay, J.M., *Drago, M.J., *Powers, T.L., *Schufreider, A.K., Connor, K.R., Reitter, J.N., and Mills, K.V. (2013) Splicing of a non-canonical class three intein from Clostridium thermocellum. FASEB J. 27, 789.5. *Urbanski, L.M., *Chin, S.L., Reitter, J.N. and Mills, K.V. (2013) Peptide bond cleavage adjacent to asparagine or glutamine. FASEB J. 27, 789.6.

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Mills, K.V., Reitter, J.N., Connor, K.R., *Nicastri, M.C., *Williams, J.E., *Colelli, K.M., and *Marieni, M.D. (2013) The structure, regulation and activity of non-canonical inteins. FASEB J. 27, 998.2. *Savidis, G., *Drago, M.J., Reitter, J.N., Mills, K.V. (2012) Expression and auto-processing of hedgehog-like domains from Brugia malayi. FASEB J. 26, 753.2. *Colelli, K.M., Reitter, J.N. and Mills, K.V. (2012) Protein splicing of a temperature-dependent intein from an extreme thermophile. FASEB J. 26, 756.3. (Honorable Mention – Best Undergraduate Poster) *Williams, J.E., Reitter, J.N. and Mills, K.V. (2012) Protein splicing facilitated by highly similar inteins from two extreme thermophiles. FASEB J. 26, 756.4. *Karanja, K.K., *Urbanski, L.M., Reitter, J.N. and Mills, K.V. (2012) Protein splicing of inteins from Synechococcus sp. PCC 7002 and Pyrococcus abyssi. FASEB J. 26, 756.5. *Urbanski, L.M., *Chin, S.L., Reitter, J.N. and Mills, K.V. (2012) Side-chain cyclization of Gln or Asn coupled to peptide bond cleavage in an intein and a model peptide. FASEB J. 26, 756.6. *Chin, S.L., *Nadelson, A.C., *Urbanski, L.M., Reitter, J.N. and Mills, K.V. (2012) Intein-mediated peptide bond cleavage adjacent to asparagine or glutamine. FASEB J. 26, 963.6. Mills, K.V., Reitter, J.N., Connor, K.R., *Nicastri, M.C., *Urbanski, L.M., *Williams, J.E., *Colelli, K.M., *Savidis, G. and *Marieni, M.D. (2012) Non-canonical inteins: Protein splicing by alternate mechanisms. FASEB J. 26, 959.2. *Nicastri, M.C., *Xega, K., Reitter, J.N. and Mills, K.V. (2012) Conditional protein splicing of inteins with a non-canonical C-terminal glutamine. FASEB J. 26, 959.1. Mills, K., Reitter, J., *Dorval, D., *Lewandowski, K., *Marieni, M., *Duffee, L., *Donahue, C. (2011) Non-canonical inteins: Alternate mechanisms for protein splicing. Protein Science 20-Sup 1, 180. *Xega, K., Reitter, J.N. and Mills, K.V. (2011) Post-translational autoprocessing by an Oryza sativa hedgehog-like domain. FASEB J. 25, 754.16. *O’Brien, K.M., *Schufreider, A.K., *McGill, M.A., *O’Brien, K.M., Reitter, J.N. and Mills, K.V. (2011) Post-translational autoprocessing of a Pyrococcus abyssi intein and a Cryptosporidium hedgehog-like domain. FASEB J. 25, 754.10. *Marieni, M.D., *Duffee, L.R., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2011) The influence of conserved catalytic residues on the mechanism of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 25, 520.10.

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*Donahue, C.A., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2011) Determining the activation barrier and pH-dependence of each step of protein splicing. FASEB J. 25, 520.6. *Chin, S.L., *Nadelson, A.C., *York, D.J., Reitter, J.N. and Mills, K.V. (2011) Utilizing a protein splicing side reaction to generate a fragment for expressed protein ligation. FASEB J. 25, 520.3. Mills, K.V., *Powers, T.L., *Drago, M.J., Connor, K.R., and Reitter, J.N. (2011) Non-canonical inteins: Alternate mechanisms for protein splicing. FASEB J. 25, 520.11. *Duffee, L.R., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2010) A kinetic analysis of each step of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 24, 463.5. Mills, K.V., Reitter, J.N., *Duffee, L.R., *York, D.J., *Dorval, D.M., and *Lewandowski, K.T. (2010) Non-canonical inteins: alternate mechanisms for protein splicing. FASEB J. 24, 520.11. *Nadelson, A.C., *York, D.J., *Dorval, D.M., *Lewandowski, K.T., Connor, K.R., Reitter, J.N. and Mills, K.V. (2010) Manipulation of protein splicing side reactions to facilitate protein purification and expressed protein ligation. FASEB J. 24, 463.13. *York, D.J., *Duffee, L.R., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2010) Estimating the activation barrier to each step of protein splicing for the non-canonical P. abyssi PolII intein. FASEB J. 24, 463.18. (Honorable Mention – Best Undergraduate Poster) *Duffee, L.R., *Nadelson, A.C., *York, D.J., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2009) Influence of conserved residues on individual steps of protein splicing of the non-canonical Pyrococcus abyssi PolII intein. Protein Science 18-Sup1, 86. Selected for an oral presentation. *Nadelson, A.C., *York, D.J., *Lewandowski, K.T., *Dorval, D.M., Connor, K.R., Reitter, J.N. and Mills, K.V. (2009) Development of protein purification strategies using the Pyrococcus abyssi PolII intein. Protein Science 18-Sup1, 86. Mills, K.V., Reitter, J.N., Connor, K.R., *Kerrigan, A.M., *Dorval, D.M., *Drago, M.J., *Lewandowski, K.T. and *Powers, T.L. (2009) Non-canonical inteins: Alternate mechanisms of protein splicing. Protein Science 18-Sup1, 171. *Schufreider, A.K., *McGill, M.M., *O’Brien, K.M., Reitter, J.N, and Mills, K.V. (2009) Protein Splicing of the Pyrococcus abyssi lon protease intein. FASEB J. 22, 502.2. (Honorable Mention – Best Undergraduate Poster)

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*Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2009) The influence of extein residues on the protein splicing of three Pyroccocus abyssi inteins. FASEB J. 22, 502.3. (Honorable Mention – Best Undergraduate Poster) *York, D.J., Nadelson, A.C., Reitter, J.N., and Mills, K.V. (2009) Alternate protein splicing mechanisms: A directed evolution approach. FASEB J. 22, 502.2. Reitter, J.N., *Schufreider, A.K., and Mills, K.V. (2009) Protein Splicing by a Non-canonical Mechanism. FASEB J. 22, 677.3. *McGill, M.A., *O’Brien, K.M., and Mills, K.V. (2008) The P. abyssi lon-b protease: Protein splicing activity and proteolysis by soluble fragments. Abstracts of the ASBMB 2008 National Meeting. *Kerrigan, A.M., *Powers, T.L., *Dorval, D.M., Reitter, J.N. and Mills, K.V. (2008) The dependence of three P. abyssi inteins on extein sequence for efficient protein splicing. FASEB J. 20, 611.13. Mills, K.V., Reitter, J.N., Connor, K.R., *Dorval, D.M., *Drago, M.J., *Lewandowski, K.T., and *Powers, T.L. (2008) Alternate mechanisms of protein splicing by non-canonical inteins. Abstracts of the ASBMB 2008 National Meeting. Mills, K.V., Reitter, J.N., Connor, K.R., *Powers, T.L. and *Drago, M.J. (2007) Protein splicing of a non-canonical intein from C. thermocellum. Abstracts of Papers, 234th ACS National Meeting, Boston, MA, BIOL-237. *McGill, M.A., *O’Brien, K.M., and Mills, K.V. (2007) Protein splicing and extein activity of the Pyrococcus abyssi lon protease intein. Protein Science 16-Sup 1, 162. Mills, K.V., Reitter, J.N., Connor, K.R., *Powers, T.L., *Drago, M.J., *Lewandowski, K.T., and *Dorval, D.M. (2007) Alternative protein splicing mechanisms of inteins from C. thermocellum and P. abyssi. Protein Science 16-Sup 1, 158. *Dorval, D.M., Connor, K.R., and Mills, K.V. (2006) Catalysis of individual steps of protein splicing of the Pyrococcus abyssi PolII intein. FASEB J. 20, A40. *Powers, T.L., *Drago, M.J., *Dorval, D.M., Connor, K.R., and Mills, K.V. (2006) Protein splicing of a non-canonical Clostridium thermocellum intein with N-terminal Gln. FASEB J. 20, A964. Mills, K.V., *Lewandowski, K.T., and *Dorval, D.M. (2005) The Mechanism of Protein Splicing of the Pyrococcus abyssi PolII intein. Protein Science 14-Sup 1, 87. *Lewandowski, K.T., *Dorval, D.M., and Mills, K.V. (2005) A Kinetic study of the protein splicing mechanism of the Pyrococcus abyssi PolII intein. FASEB J. 19, A1401.

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Mills, K., *Manning, J., *Garcia, A., *Wuerdeman, L. (2004) In vitro protein splicing of an intein with a C-terminal glutamine from Pyrococcus abyssi. FASEB J. 18, C276. *Lewandowski, K., *Wuerdeman, L., *Twomey, P., and Mills, K. (2004) Murine Sonic Hedgehog proteins: Sub-cloning, over-expression in E. coli and affinity purification. FASEB J. 18, C277. Invited Research Seminars Loyola University, Department of Chemistry, Baltimore, MD, February 10, 2015 Biophysical Society 59th Annual Meeting, Baltimore, MD, February 8, 2015 FASEB/ASBMB National Meeting 2014, San Diego, CA, April 27, 2014 Union College, Department of Chemistry, Schenectady, NY, October 11, 2012 Wellesley College, Biochemistry, Wellesley, MA, September 19, 2012 FASEB/ASBMB National Meeting 2010, Anaheim, CA, April 28, 2010 Pomona College/Harvey Mudd College, Claremont, CA, April 27, 2010 University of Massachusetts, Department of Chemistry, Amherst, MA, March 4, 2010 University of Vermont, School of Medicine, Burlington, VT, April 17, 2009 Trinity College, Hartford, CT, May 30, 2008 Worcester Polytechnic Institute, Worcester, MA October 12, 2005 Clark University, Worcester, MA April 1, 2002 New England Biolabs, Beverly, MA October 11, 2001 Gordon Research Conference, Biological Regulation Mechanisms Holderness, NH, July 30-August 4, 2000 Other Selected Professional Presentations, Poster Presentations FASEB/ASBMB 2015, Boston, MA, March 29, 2015 Winter Enzyme Mechanism Conference, Galveston, TX, January 5, 2015 28th Symposium of the Protein Society, San Diego, CA, July 29, 2014

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FASEB/ASBMB 2014, San Diego, CA, April 26, 2014 27th Symposium of the Protein Society, Boston, MA July 20, 2013 FASEB/ASBMB 2013, Boston, MA, April 23, 2013 25th Anniversary Symposium of the Protein Society, Boston, MA July 26, 2011 FASEB/ASBMB 2011, Washington, DC, April 10, 2011 Dreyfus Foundation, New York, NY, October 30, 2010 FASEB/ASBMB 2010, Anaheim, CA, April 25, 2010 23rd Symposium of the Protein Society, Boston, MA, July 25-29, 2009 Gordon Research Conference, Enzymes, Coenzymes and Metabolic Pathways University of New England, Biddeford, ME, July 2008 IUBMB/ASBMB 2008, San Diego, CA April 8, 2008 The 234th American Chemical Society National Meeting, Boston, MA, August 21, 2007 Twenty-First Symposium of the Protein Society, Boston, MA July 21-July 25, 2007 Nineteenth Symposium of the Protein Society, Boston, MA July 30-August 3, 2005 IUBMB/ASBMB 2004, Boston, MA June 15, 2004 Gordon Research Conference, Biological Regulation Mechanisms Holderness, NH, July 30-August 4, 2000

Thirteenth Symposium of the Protein Society, Boston, MA, July 24-28, 1999 Student Research Mentoring Summer Research Students Summer 2015 Alexander Bonano (Chemistry ’17) Christopher Cousin (Chemistry ’16) Joshua Long (Biology ’17) Christopher Minteer (Chemistry/Biochemistry ’17) Isabelle Pierre (UMass-Dartmouth, ’15) Christian Ramsoomair (Chemistry ’18)

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Summer 2014 Alexander Bonano (Chemistry ’17, NSF) Dawilmer Castillo (’15, Bridgewater State NSF) Michael Koulopoulos (Chemistry/Biochemistry ’16, Holy Cross) Zachary Giaccone (Chemistry/Biochemistry ’15, NSF) Mario Jaramillo (Biology ’15, NSF) Anthony Yakely (Chemistry/Biochemistry ’16, Dreyfus) Summer 2013 Julliard del Rosario, ’16 Cornell University (NSF) Zachary Giaccone (Chemistry/Biochemistry ’15) Daniel Haley (Chemistry ‘14) Mario Jaramillo (Biology ’15) Nicolle Siegart (Biology/Biochemistry ’15) Summer 2012 Sarah Cahn (Chemistry ’14, Becton Dickinson) Kathryn Colelli (Biology/Biochemistry ’13, Beckman Foundation) Nick Gulbrandsen (Chemistry ’13, Fitchburg State) Jennifer Pusztay (Chemistry/Biochemistry ’13, NSF) Nicolle Siegart (Biology ’15, NSF) Summer 2011 Kathryn Colelli (Biology/Biochemistry ’13, Beckman Foundation) Kevin Karanja (Worcester State Chemistry, ’12, NSF) Michael Nicastri (Chemistry/Biochemistry ’13, Holy Cross) Laura Urbanski (Chemistry/Biochemistry ’13, Holy Cross) Jennie Williams (Chemistry ‘13, NSF) Summer 2010 Stacy Chin (Chemistry ’12, NSF) Colleen Donahue (Biology ’11, NIH) Michelle Marieni (Biology ’11, NSF) George Savidis (Biology/Biochemistry ’12, Dreyfus) Kristina Xega (Chemistry/Biochemistry ’11, NSF) Summer 2009 Lauren Duffee (Biology/Biochemistry ’10, NSF) Adam Nadelson (Biology ’10, NSF) Kevin O’Brien (Biology/Biochemistry ’11, NSF) Daniel York (Biology/Biochemistry ’10, NSF) Summer 2008 Adam Nadelson (Biology ’10, NSF) Daniel York (Biology/Biochemistry ’10, NSF)

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Summer 2007 Adam Kerrigan (Biology ’09, NSF) Melissa McGill (Biology/Biochemistry ’08, NSF) Ann Schufreider (Chemistry ’09, NSF) Jennifer Winslow (Biology ’09, NSF) Summer 2006 Deirdre M. Dorval (Biology ’06, NSF) Melissa McGill (Biology/Biochemistry ’08, NSF) Kathryn O’Brien (Chemistry/Biochemistry ’08, NSF) Summer 2005 Matthew Drago (Biology/Biochemistry ’07, NSF) Deirdre M. Dorval (Biology ’06, Research Corporation) Taryn Powers (Biology ’07, NSF) Summer 2004 Deirdre M. Dorval (Biology ’06, Research Corporation) Katherine Lewandowski (Biology/Biochemistry ’05, Simeon J. Fortin Charitable Trust) Summer 2003 Jaclyn Gareau (Chemistry/Biochemistry ’04, Simeon J. Fortin Charitable Trust) Katherine Lewandowski (Biology/Biochemistry ’05, Simeon J. Fortin Charitable Trust) Summer 2002 Alicia M. Garcia (Biology ’03, Simeon J. Fortin Charitable Trust) Jennifer S. Manning (Chemistry ’03, Simeon J. Fortin Charitable Trust) Timothy D. Quinn (Chemistry/Biochemistry ’04, Pfizer, PREPARE) Lisa A. Wuerdeman (Chemistry ’03, Simeon J. Fortin Charitable Trust) Academic Year Research Students 2015-2016 Christopher Cousin (Chemistry ’16) Chemistry Research I Michael Koulopoulos (Chemistry/Biochemistry ’16) Chemistry Research II/Biochemistry Thesis Joshua Long (Biology ’17) Chemistry Research I Christopher Minteer (Chemistry/Biochemistry ’17) Chemistry Research I Christian Ramsoomair (Chemistry ’18) Introduction to Research Anthony Yakely (Chemistry/Biochemistry ’16)

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Chemistry Research II/Biochemistry Thesis 2014-2015 Alexander Bonano (Chemistry ‘17) ALANA Mentoring Program Vanessa Costa (UNDL ’18) First year Research Advancement Program Christopher Cousin (Chemistry ’16) Introduction to Research Zachary Giaccone (Chemistry/Biochemistry ’15) Chemistry Research II/Biochemistry Thesis Michael Koulopoulos (Chemistry/Biochemistry ’16) Chemistry Research I Mario Jaramillo (Biology ’15) Chemistry Research II Christian Ramsoomair (UNDL ’18) First year Research Advancement Program Nicolle Siegart (Biology/Biochemistry ’15) Chemistry Research II/Biochemistry Thesis Anthony Yakely (Chemistry/Biochemistry ’16) Chemistry Research I 2013-2014 Alexander Bonano (Chemistry ‘17) ALANA Mentoring Program Sarah Cahn (Chemistry ’14) Chemistry Research II Zachary Giaccone (Chemistry/Biochemistry ’15) Chemistry Research I Daniel Haley (Chemistry ‘14) Chemistry Research I Mario Jaramillo (Biology ’15) Chemistry Research I Nicolle Siegart (Biology/Biochemistry ’15) Chemistry Research I 2012-2013 Sarah Cahn (Chemistry ’14) Chemistry Research I Kathryn Colelli (Biology/Biochemistry ’13) Chemistry Research II/Biochemistry Thesis Michael Nicastri (Chemistry/Biochemistry ’13) Chemistry Research II/Biochemistry Thesis Jennifer Pusztay (Chemistry/Biochemistry ’13) Chemistry Research II/Biochemistry Thesis Nicolle Siegart (Biology ’15)

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Introduction to Research Laura Urbanski (Chemistry/Biochemistry ’13) Chemistry Research II/Biochemistry Thesis/Honors Thesis Jennie Williams (Chemistry ’13) Chemistry Research II 2011-2012 Stacy Chin (Chemistry ’12) Chemistry Research II Kathryn Colelli (Biology/Biochemistry ’13) Chemistry Research I Kevin Karanja (Chemistry-Worcester State ’12) Michael Nicastri (Chemistry/Biochemistry ’13) Chemistry Research I Jennifer Pusztay (Chemistry/Biochemistry ’13) Introduction to Research George Savidis (Biology/Biochemistry ’12) Chemistry Research II/Biochemistry Thesis Laura Urbanski (Chemistry/Biochemistry ’13) Chemistry Research I Jennie Williams (Biology ’13) Introduction to Research 2010-2011 Stacy Chin (Chemistry ’12) Chemistry Research I Colleen Donahue (Biology ’11) Chemistry Research I Michelle Marieni (Biology ’11) Chemistry Research I Kevin O’Brien (Biology/Biochemistry ’11) Chemistry Research II George Savidis (Biology/Biochemistry ’12) Chemistry Research I Jennie Williams (Biology ’13) Introduction to Research Kristina Xega (Chemistry/Biochemistry ’11) Chemistry Research I 2009-2010 Lauren Duffee (Biology/Biochemistry ’10)

Chemistry Research II/Biochemistry Thesis Adam Nadelson (Biology ’10)

Chemistry Research II Kevin O’Brien (Biology/Biochemistry ’11) Chemistry Research I

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Kristina Xega (Chemistry/Biochemistry ’11) Introduction to Research Daniel York (Biology/Biochemistry ’10)

Chemistry Research II/Biochemistry Thesis 2008-2009 Lauren Duffee (Biology/Biochemistry ’10) Introduction to Research Adam Kerrigan (Biology ’09)

Chemistry Research II Adam Nadelson (Biology ’10)

Chemistry Research I Ann Schufreider (Chemistry ’09)

College Honors Jennifer Winslow (Biology ’09)

Chemistry Research II Daniel York (Biology/Biochemistry ’10)

Chemistry Research I 2007-2008 Adam Kerrigan (Biology ’09)

Chemistry Research I Melissa McGill (Biology/Biochemistry ’08)

Chemistry Research II/Biochemistry Thesis Kathryn O’Brien (Chemistry/Biochemistry ’08)

Chemistry Research II/Biochemistry Thesis Ann Schufreider (Chemistry ’09)

Chemistry Research I Jennifer Winslow (Biology ’09)

Chemistry Research I 2006-2007 Matthew Drago (Biology/Biochemistry ’07)

College Honors/Biochemistry Thesis Melissa McGill (Biology/Biochemistry ’08)

Chemistry Research I Kathryn O’Brien (Chemistry/Biochemistry ’08)

Chemistry Research I Taryn Powers (Biology ’07)

Chemistry Research II 2005-2006 Deirdre Dorval (Biology ’06)

College Honors Matthew Drago (Biology/Biochemistry ’07)

Chemistry Research I

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Taryn Powers (Biology ’07) Chemistry Research I

2004-2005 Deirdre Dorval (Biology ’06)

Chemistry Research I Katherine Lewandowski (Biology/Biochemistry ’05)

Chemistry Research II/Biochemistry Thesis 2003-2004 Jaclyn Gareau (Chemistry/Biochemistry ’04)

Chemistry Research II/Biochemistry Thesis/Departmental Honors Kathleen Leahy (Mathematics ’04) College Honors (on-campus advisor) Katherine Lewandowski (Biology/Biochemistry ’05) Chemistry Research I Timothy Quinn (Chemistry/Biochemistry ’04) Chemistry Research II/Biochemistry Thesis/Departmental Honors 2002-2003 Alicia Garcia (Biology ’03) College Honors Jaclyn Gareau (Chemistry/Biochemistry ’04) Chemistry Research I Timothy Quinn (Chemistry/Biochemistry ’04) Chemistry Research I Jennifer S. Manning (Chemistry ’03) Chemistry Research I Ralph M. Millilo (Biology ’03) with Prof. R. Bertin Biology Research/Biochemistry Thesis Lisa Wuerdeman (Chemistry ’03) Chemistry Research I 2001-2002 Beth Ambrose (Chemistry ’02) Chemistry Research I Patrick Twomey (Chemistry/Biochemistry ’02) Chemistry Research I/Biochemistry Thesis Jennifer S. Manning (Chemistry ’03) Unofficial Chemistry Research Lisa Wuerdeman (Chemistry ’03) Unofficial Chemistry Research

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College Honors Program Thesis Reader Katherine Beckwith (Biology ’03), 2003 Cristiana Pineda (Chemistry ’12), 2012 Selected College Service/Professional Development 2013- STEM-E working group, co-founder 2013 Search Committee, Director of Sponsored Research 2011-2013 Finance and Planning Council 2013 Independent Colleges Office Liaison Meeting participant 2013-2014 Colleges of Worcester Consortium Leadership Fellows Program 2013 Ad hoc Montserrat Writing Assessment Committee 2013 Ad hoc AAC subcommittee on faculty research 2011-2012 Presidential Colloquium on Jesuits and the Liberal Arts 2008-2010 Committee on Faculty Affairs, Chair, 2009-10 Subcommittee Chair, Handbook on Tenure and Promotion Subcommittee on Appeals 2008-2009 Science Ambassadors Faculty Advisor 2008-2010 Faculty mentorship program 2008-2009 NEASC Review Subcommittee on Governance 2008-2010 Faculty Mentorship Program 2010 Beckman Scholars selection committee 2007 “Being a Holy Cross Student” Gateways Session Leader 2004-2006 Academic Affairs Council, Priorities Committee 2004-2006 Curricular Review Steering Committee 2001-present Biochemistry Concentration, Co-Chair 2002-2003, 2004-2007 Committee for Graduate Studies and Fellowships 2002-2003 Curricular Goals Committee: Acquisition, integration and application of knowledge 2006-07, 10-12 Presidential Service Award Selection Committee 2002 Undergraduate Summer Research Symposium, co-chair 2002-2004, 2007 Summer Gateways Advisor 2002-present Founder/organizer of summer biomolecular summer student research student journal club 2004 Presented talk on “Mad Cows and Cannibals” for

summer research program 2004-2006 PAL host for Atoms and Molecules 2005 Ad hoc Classroom of the Future committee 2003-2006, 2008 Organized student group trip to MIT Center for Cancer

Research Annual Symposium 2002-2007 Founded/organized annual summer student/faculty softball

game series (w/ G. Roberts)

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Selected Department Service 2009 Revised and lead department seminar program as teaching overload 2004-2010 General Chemistry laboratory faculty director 2005-2007 New Chemistry building planning, committees for Material Management,

General Chemistry lab, Biochemistry research and Non-synthetic Research

2001-2002 Cross and Crucibles faculty advisor 2001,02,05.07 Participated in Fall Parent’s Weekend Open House 2004, 12 Hosted prospective student department session 2003,04,07,12 Hosted departmental Gateways browsing sessions 2001-present Hiring committees for ten faculty and four lab coordinators 2003 Co-wrote Departmental Academic Honesty Policy 2001 Graduate School advising session panelist 2001-present Biochemistry coordinator 2001-present Hosted prospective student summer visits to the department Selected Community Service 2015 Panelist, Massachusetts STEM summit, Proven Strategies in Fostering and

Retaining STEM Students 2014 NSF Review Panel, Division of Undergraduate Education (Apr 2014) 2012-16 Wachusett Regional School Committee member 2012-2013: Subcommittees on Education and Community Outreach 2012-: Davis Hill Elementary School Improvement Council 2013: Subcommittees on Management and Legal Affairs (Chair) 2012 Colleges of Worcester Consortium, Faculty panelist, “Planning Successful Scholarship: Obtaining Fellowships and Grants for Sabbaticals and Ongoing Research” (11/2/12) 2011 PKAL Learning Spaces Collaboratory Regional Workshop Panelist 2011 NSF Review Panel, Molecular and Cellular Biosciences (Apr 2011) 2010 NSF Review Panel, Molecular and Cellular Biosciences (Apr 2010) 2010 Chemistry demonstration to 50 4-6 year olds, Goddard School, Auburn,

MA 2010,11,14 Poster judge at undergraduate poster session, ASBMB national meeting 2009 NSF Review Panel MRI-PUI, Chemistry (Oct 2009) 2007- Ad hoc reviewer for Protein Science, Biochemistry, National Science

Foundation MCB, Chembiochem, Journal of Chemical Education, IUBMB Life, BMC Bioinformatics, Journal of Bacteriology, PLoS One, Acta Biochimica et Biophysica Sinica, Nature Biotechnology, Nature Chemical Biology, Frontiers in Microbiology

2009 Poster session judge at Protein Society meeting 2008-present Founder and Advisor, Holy Cross Undergraduate Affiliate Network of

American Society for Biochemistry and Molecular Biology 2006 Seminar to post-doc association at UMass Medical School on career

options in liberal arts colleges

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2005 Participated as panelist in Science faculty forum by Consortium on High Achievement and Success, Mt. Holyoke College

2005 Presented talk to Johns Hopkins Center for Talented Youth Odyssey Series

2005 Panelist for HHMI-Boston area alumni group career options discussion 2001-2002 United Way Fund Drive Department Coordinator 2002 Relay for Disaster Relief participant Professional Membership 2008-present Project Kaleidoscope, F21 2000-present American Chemical Society, Biological Chemistry Division 2000-present American Society for Biochemistry and Molecular Biology 2005-present Protein Society 2001-present Council on Undergraduate Research 1995-present Phi Beta Kappa 1995-present Alpha Sigma Nu (National Jesuit Honor Society) 2010-present American Society for Microbiology