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Kenneth DawsonCentre for BioNano Interactions
EMEA ITF Group on NanotechnologiesApril 29th 2009
Website: www.cbni.eu
BioNanoInteractionsArea(s) of expertise
Nanoparticle synthesis Protein Corona
Transcriptomics and Proteomics
Disease / therapy
In vivo effects
IntracellularVisualization
Dispersion and characterisation
GLP
Class III nanoparticle synthesis
Class III Protein ExpressionClass III Cell Culture
New Institute,Integrated NanoAnd Biology, Good Laboratory Practice (?)
160-200 people
Protein Corona paradigm
Nanoparticles utilise existing biological pathways (in new ways?)
Uptake connected to protein corona
Nanotechnology for the Pharmaceutical industry
1
2 6
34
5
K15=[5][1]
0
50
100
150
200
250
0 50 100 150 200 250
Exposure time, minutes
FITC
log
37C 4C NaN3
1
2
3
65
4 0
5
10
15
20
25
30
0 50 100 150 200 250 300
Exposure time, minutes
FITC
log
37C 4C NaN3
Completely different uptake behaviour
0 30 60 90 120 150 180 210 240
0
5
10
15
20
25
30
35
Cel
l Flu
ores
cenc
e In
tens
ity
Exposure time, minutes
37C 4C NaN3
Distinct localisation in ER
Clear energy dependence
Quality And Standards Issues
Sds-gel (4% stacking gel and 10% resolving gel)
particles
Free dye (<6 kda)
Much of the literature in this area questionable!
Also observed for FITC-dextrans etc., not only nanoparticles.
Commercial samples leak - free dye!
new methods in-situ characterization
NanoSight Spinning Disc Centrifugation
SiO2 nanoparticles with increasing plasma concentration
Rel
Wei
ght
NIST Gold nanoparticles 30nm and 60nm in citrate buffer
