Aminoglycoside ototoxicity and hair cell ablation in the adult gerbil:A simple model to study hair cell loss and regenerationDisusun Oleh:Alifa AssyifaH2A010002FAKULTAS KEDOKTERANUNIVERSITAS UHAADI!AH SEARAN"KE#ANITERAAN KLINIK $A"IAN THT RSUD A$ARA%ALa&a' $ela(an)An&i*i+&i(a,in+)li(+si-a(e&i(a-i)una(an-ala,&e'a.i(+,*inasi -en)anl++.-iu'e&i/se.e'&iasa,e&ha/'yni/se'in),enye*a*(an&uli 'e0e'si*el 1$'+2n e& al3415678 a&h+) e& al34 1595:Se*a)ian(e'usa(an(a'ena(e,a&ianselsens+'is-a'ie.i&el+')an ;+'&i4&e'le&a(-i&e,.u'un)&ulan)(+(lea3Des&'u(si-a'i&i)a*a'issel lua''a,*u&-ansa&u*a'issel-ala,'a,*u&,enye*a*(an ,enu'unnyafun)sisel.en-u(un)se(i&a'-an.en))an&ian+')an -en)an e.i&el -a'i ?a'in)an .a'u&3Ke'usa(an+')an;+'&ise'in)-ise'&ai,enu'unnyafun)sineu'+n )an)li+ns.i'al1S"Ns:yan),en)ine'0asisel'a,*u&3enu'unnya fun)sineu'+nini&e'?a-i-en)an0a'iasiyan)/e.a&.a-as.esiesyan) *e'*e-a3"e'*il-a.a&-i)una(anse*a)aisu*?e(4.en))unaana,in+)li(+si-a 1(ana,isin:-+sis&un))al-ii(u&isa&u-+sisl++.-iu'e&i/fu'+se,i- -a.a&,en)in-u(si.enu'unan.en-en)a'anse/a'a/e.a&-an .e',anene&+-e -an $ahan"e'*il +n)+lia4 u,u' @ A B *ulan4 ?an&an -an *e&ina &i-a( a-a .e'*e-aanIn?e(si s/ la'u&an )en&a,isin sulfa&C(ana,isin sulfa& -ala, n+',al saline 1700AB00,)C():Se&elah 20A@0 ,eni&In?e(si in&'a.e'i&+neal *u,e&ani-e1B0,)C():Cla'u&an fu'+se,i- 1100,)C():He2an +-el O&+&+(sisi&asA.li(asi &+.i(al uC (+(leaanes&esi -iin-u(si -a'i is+=u'ane4 *ulla au-i&+'i -i/a.ai ,elalui insisi 'e&'+au'i/ula,en))una(an s/al.el A,in+)li(+si-a -an la'u&an -iu'e&i/ -ia.li(asi(an se/a'a lan)sun) -ise(i&a'nyae&+-e -an $ahanen))una(an @ .a(e& .'+ses si)nal -i)i&al .e'an)(a& (e'as -an luna(un&u( ,enun?u((an ?an)(auan110 -$ D 20 -$Ran)san)an *unyi ,e'u.a(an *unyi ,u'ni B ,s .a-a E0 -$#en)u(u'an 'es.+n *a&an) +&a( au-i&+'i -i'e(a, ,en))una(an 26" ele(&'+-a ?a'u, su*-e',al yan) -i&e,.a&(an -i 0e'&eF &en)(+'a( 1ele/&'+-e .e'e(a,: -an .'+ses ,as&+i- i.sila&e'al 1ele(&'+-a 'u?u(an:4 -en)an ele(&'+-a .+(+( -i&e,.a&(an -i *a)ian *ela(an) -e(a& e(+'3 S"Ns -i (anal R+sen&hal -i2a'nai GIIIATu*ulin -an -ihi&un) -a'i )a,*a' yan) -ia,*il .a-a Heiss AFi+.h+& ,i/'+s(+.e$a)ian a.e(s4 .e'&en)ahan -an *asal *e')an&ian -ihi&un)3 Densi&as sel -ihi&un) -en)an ,en)u(u' -ae'ah (anal R+sen&hal ,en))una(an FIII -an ,en)hi&un) ?u,lah sel .e' ,,2U?i ANOVA sa&u a'ah -an &A&es &i-a( *e'.asan)an un&u( si)nif(ansi#en)u(u'an .en-en)a'an#en)hi&un)an -ensi&as )an)li+n s.i'alHasilA.li(asi )en&a,isin &+.i(al &i-a( ,enye*a*(an hilan)nya sel 'a,*u& (+(lea#a-a )e'*il ,enun?u((an &i-a( a-a (e'usa(an yan) ?elas -a'i selAsel 'a,*u&3#e'sa'afan -a'i +')an ;+'&i -iu?i -en)an sa'af ,a'(e' GAIII &u*ulin &i-a(a-a(e'usa(annya&a-a'i.e'sa'afan(eselAsel'a,*u& *a)ian -ala, se&elah .en)+*a&an )en&a,isin3 Ti-a( a-a in-i(asi fen+&i.e .e'ila(u &+(sisi&as 0es&i*ula'3HasilA.li(asi )a*un)an -a'i (ana,isin -an *u,e&ani-e hanya ,en)in-u(si (e'usa(an 'in)an#en)+*a&an &+.i(al &i-a( ,en)a(i*a&(an hilan)nya .en-en)a'an3A,*an).en-en)a'an-i*an)(i&(an-a'i20-$hin))a26-$ sela,a.e'/+*aan-ananalisis-a'i'es.+n&e'ha-a.f'e(uensi na-a,u'ni,enun?u((an*ah2a(e'usa(anini&e'?a-i&e'u&a,a -i(isa'an.e'&en)ahanf'e(uensi4-en)an1EA@7(HJyan).alin) *e'.en)a'uh3Hasil#en)+*a&an -en)an (ana,isin -an fu'+se,i-e ,enun?u((an hilan)nya sel 'a,*u& su*s&ansial #a-a)e'*il-i+*a&i-en)an-+sis&un))al(ana,isinsa?a4&i-a( a-a*u(&ihilan)nyasel'a,*u&yan)-ia,a&i1-a&a&i-a( -i&un?u((an:3Na,un4(e&i(a-+sis&un))al4-+sis(ana,isin sis&e,i(yan)-if+ll+2u.&a(la,a-anse&elahi&u,en-a.a& sun&i(anfu'+se,i-e4a-a(e'usa(anyan)/e.a&-an*esa'*ai( 'a,*u& -ala, -an lua' sel3Se,en&a'a i&u4 .a-a 19 ,in))u -an @7 ,in))u .as/a .e'a2a&an4 +')an /+'&i &elah *e'-e)ene'asi 1(+la.s:3A-anya.'+sesafe'ense.an?an)neu'+n)an)li+ns.i'al&e&a. u&uh*ah(anE*ulanse&elah+&+&+(si(a(u&-anhilan)nyasel 'a,*u&3HasilSel 'a,*u& -ihi&un) se&elah sa&u ,in))u .en)+*a&an (ana,isin C fu'+se,i-e#en)hi&un)ananalisis.e2a'naan-en)ani,,un+'ea/&i0i&y ,enun?u((an (e'usa(an luas *ai( -ala, -an selAsel 'a,*u& lua'4 -i&un?u((an+lehhilan)nya.e2a'naan!O6A-ise,ua&in)(a& (+(lea3HasilNeu'+n )an)li+n s.i'al yan) -ia2e&(an se&elah (e,a&ian sel 'a,*u&Di&e,u(an*ah2aa-ase-i(i&*a-anselneu'+n)an)li+ns.i'al yan)hilan)-i(analR+sen&hal4*ah(ansa,.aiE*ulanse&elah .e'a2a&an4 &e'-a.a& a-anya )an))uan .en-en)a'an .a-a he2an yan) &elah -i(+nf',asi +leh .enin)(a&an a,*an) *a&as A$R sa&u ,in))u se&elah .en)+*a&an (ana,isin C fu'+se,i-e3Kesi,.ulan#en)+*a&an(+,*inasi(ana,isin-anfu'+se,i-e-an-en)an 'e)i,en-+sis&un))al&e'se*u&/u(u.un&u(,en)in-u(si)an))uan .en-en)a'anyan)/e.a&-an,en-ala,yan)&i-a(,e,*ai(-a'i 2a(&u (e 2a(&u3"en&a,isin ,e,ili(i se-i(i& efe( +&+&+(si( -ala, ,+-el ini3#+&ensiasi+&+&+(sisi&as-a'ia,in+)li(+si-a-en)anl++.-iu'e&i( *e')an&un).a-a(e.e(aans.esiesAs.esif(-ans.esiesyan) *e'*e-a,e'es.+n-en)an/a'ayan)*e'*e-a-ala,,e'es.+n a,in+)li(+si-a3Se*a)ian(e'usa(an(a'ena(e,a&ianselsens+'is(hususnyasel e.i&el+')an;+'&i-an-e)ene'asise(un-e'-a'isele.i&elsens+'i ,enun?u((anhanyaa-ase-i(i&a&au&i-a(a-a(e'usa(anneu'+n ,es(i.un .a-a E *ulan se&elah (e'usa(an sel 'a,*u&3TERIAKASIH