JPK Accessories

Accessories HAndbook

Unlimited possibilities

for NanoWizard®, ForceRobot® and CellHesion® systems

valid from 2015

Unmatched flexibility & modularityMore than 100 accessories

More than 30 operating modes

Optional headsCellHesion® 200CellHesion® 200 ForceRobot® 300ForceRobot® 300

Heads

Software Experiment Planner™

Experiment Control™

QI™ Advanced

Manipulation & lithography

Advanced AC modes

Advanced Force

Spectroscopy

Direct Overlay™

SPM COntrOl & DAtA PrOCeSSingDATA Processing

Stages Motorized precision stage life sciences stage HybridStage™ Standard stage HCS™

Heating cooling stage

ECCell™ – Electrochemisty cell

temperature control

PetriDishHeater™ CoverslipHolderBioCell™ eCCell™ Electrochemistry cell

HCM™ Heating cooling module

HtHS™ High temperature

heating stage

Typical BioScience applications and research fields:

High resolution imaging in air and liquid of single molecules such as proteins, DNA/RNA, carbohydrates, lipids, Live Cell Imaging, bacteria and virus characterization, cellular adhesion and cytomechanics, molecular interactions, receptor-ligand, antibody-antigen, molecular unfolding, molecular recognition, biomaterials, compound and implant characterization, material properties such as adhesion, elasticity, hardness; drug formulation, encapsulation and aging studies and many more

Setups and accessories for BioScience applications highlighted with ligHt BlUe BOXeS

Typical NanoScience applications and research fields:

High resolution imaging in air and liquid of single molecules, thin films, nanoparticles or nanowires, surface properties characterization such as adhesion, stiffness, friction, conductivity, charges, hardness; polymer science, single molecule force measurements, smart organic materials, surface properties of conjugated polymers, biosensors, degradable materials, electrochemistry, colloidal probe, surface forces and interface phenomena; and many more

Setups and accessories for nanoScience applications highlighted with grAY BOXeS

For more detailed information about all options see the brochures and product notes or visit www.jpk.com.

Control electronics options

Vortis™ SPMControlVortis™ SPMControlVortis™ Advanced

SPMControlVortis™

Combi controller SPMControlCombi controller

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NanoWizard®NanoWizard®NanoScience and BioScience versions

NanoWizard®NanoWizard®NanoOptics fiber port and UV transparency versions

NanoWizard® ULTRA SpeedNanoWizard® ULTRA Speedfor fast-scanning and super-resolution AFM

electrical modes & application modules

High Voltage Sample Bias Amplifier

Conductive AFM

module

tuning fork

module

CoverslipHolder with electrical

sample connection

HyperDrive™ fluid imaging

package

Fast scanning package

Kelvin Probe

module

StM module

extras & OeM 8x FluidicsModule™

Acoustic enclosure

Vibration isolation

Fiber coupled detection moduleMicromanipulator Cameras

Fluid cells & cantilever holders

Small volume SmallCell™

Standard Cantilever

Holder

Side-view Cantilever

Holder

PetriDish Holder

Cantilever Holder with electrical

tip connection

Supercut Cantilever

HolderSmallCell™

BioMAT™ CellHesion® Module

System options TopView Optics™

TAO™

Module

2-axes3-axes

the NaNoWizard®, Forcerobot® aNd cellhesioN® Family

Can also drive the JPK NanoTracker™

PetriDishHeater™ for BioMAT™

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Contents

1 Control electronics options 4 Vortis™ SPMControl Station Vortis™ Advanced SPMControl Station Vortis™ Combi controller

2 System options 5 NanoWizard® 4 for high resolution and fast scanning NanoWizard® ULTRA Speed for fast-scanning and

super-resolution AFM NanoWizard® NanoOptics head in two version ForceRobot® 300 CellHesion® 200 TAO™ Tip Assisted Optics module BioMaterials Workstation BioMAT™ CellHesion® module

3 Software modules 7 ExperimentControll™ ExperimentPlanner™ software module QI™ Advanced software module Advanced AC modes module DirectOverlay™ module NanoLithography/NanoManipulation module MicroRheology module Advanced Force Spectroscopy module

4 Applications modules and modes 8 Fast scanning package HyperDrive™ fluid imaging package OEM micropipettes Electrical sample connection module CoverslipHolder with electrical sample connection Kelvin Probe (KPM) and Scanning Capacitance (SCM)

Microscopy module Conductive AFM module Conductive AFM module – Enclosed Volume Tunneling Current Conductive AFM (TC-CAFM) module Scanning Tunnelling Microscopy (STM) module Tuning Fork module High Voltage Sample Bias Amplifier JPK 8x FluidicsModule™ ForceWheel™

5 Sample stages 11 HybridStage™ Motorized precision stage Manual precision stage Sample holder for large samples Standard stage

6 Cantilever holder options 12 Fixed-spring cantilever holder Standard cantilever holder Supercut version cantilever holder Cantilever holder with electrical tip connection

Side-view cantilever holder Magnetic field cantilever holder

7 Environmental control options – fluid cells and temperature controls 14

BioCell™ CoverslipHolder PetriDishHeater™ PetriDishHeater™ for BioMAT™ PetriDishHolder™ OEM Humidifier from LIS SmallCell™ for harsh environments 3-port small volume SmallCell™ High Temperature Heating Stage (HTHS™) Heating Cooling Module (HCM™) Heating Cooling Stage (HCS™) Cryostage

8 Electrochemistry solutions 17 Temperature controlled ECCell™ Heating Cooling Stage (HCS™) with electrochemistry cell OEM Potentiostats from IPS

9 Vibration and acoustic isolation 18 Active vibration isolation from Accurion Active vibration isolation from Table Stable Active air damping tables from TMC JPK acoustic enclosure JPK base for acoustic enclosure

10 Optics accessories 19 TopViewOptics™ TopViewOptics™ with boom stand TopViewOptics™ for use with inverted optical microscopes

or the JPK BioMAT™ Raman reflector kit Upright Fluorescence Microscope (UFM) Kit Fiber coupled detection module Inverted optical microscope configurations

11 OEM solutions and small parts 20 Stand for AFM head JPK sample holder for small samples JPK cable anchorage pillow JPK cantilever changing tool JPK biocompatible glue JPK spare parts OEM syringe pumps AFM cantilevers, substrates and standards CCD, EMCCD and sCMOS cameras OEM indentation solutions OEM materials testing modules for mechanical properties OEM incubators for Live Cell experiments under

controlled environments OEM glove box systems for NanoWizard® AFMs

accessories haNdbook

Control electronics options1

Vortis™ SPMControl StationState-of-the-art digital controller with lowest noise levels.

Key features

High speed 16bit AD conversion with 60 MHz for the photodetector signals 24 bit ultra precise ADC with 2.5 MHz High speed lock-in amplifier technology for

precise amplitude and phase detection High speed data capture with optional

burst mode

Modular hybrid analog/digital design with latest FPGA/PPC technology (PowerPC @ 660 MHz/FPGAs @ 240 MHz) Gigabit Ethernet interface for fast data link Number of data points that can be captured

continuously: restricted only by HDD Thermal noise acquisition up to 3.25 MHz

Vortis™ Combi controllerThe combined high-end solution for JPK Scanning Probe Microscopes and Optical Tweezers systems. (see product note)

Vortis™ Advanced SPMControl StationThe most advanced SPM controller. (see product note)

Key features

State-of-the-art digital controller with lowest noise levels and highest number of signal channels 4 high speed 16 bit ADC channels with 60 MHz 12 ADCs with 18 bit with 800 kHz 24 bit ultra precise ADC with 2.5 MHz 3 channels 14 bit DAC with 120 MHz 8 channels 24 bit DAC with 400 kHz 2 high-speed and 1 mid-speed lock-in amplifiers

for precise amplitude and phase detection

High speed data capture Modular hybrid analog/digital design with latest

FPGA/PPC technology (PowerPC @ 660 MHz / FPGAs @ 240 MHz) Gigabit Ethernet interface for fast data link Number of data points that can be captured

continuously: restricted only by HDD Thermal noise acquisition up to 3.25 MHz 2 gated photon counters

Key features

One system for all nano-force applications Simple conversion from SPM to OT control 4 high speed 16 bit ADC channels with 60 MHz 12 ADCs with 18 bit and 800 kHz 3 ADCs with 18 bit and 100 kHz (for scanning) 24 bit ultra precise ADC with 2.5 MHz 3 high speed channels 14 bit DAC and 120 MHz 8 channels 24 bit DAC with 400 kHz 2 channels 16 bit DAC and 1.6 MHz 1 × 14 bit DDS with 240 MHz +/- 1 V @ 50Ω 3 × 16 bit reference output up to 15 MHz 2 high speed lock-in amplifiers running with

60 MHz, frequency range from 1kHz to 10MHz

1 mid speed lock-in amplifiers with 800 kHz, frequency range up to 300 kHz Multiple feedback loops are implemented with fully

configurable transfer function Phase-locked-loop (PLL) integrated for Frequency

Modulation Thermal noise acquisition up to 3.25 MHz Software controlled digital filters High speed data capture 3 channels closed-loop control via capacitive

sensors 3 channels -20 to +120 V scanner drive

(4. channel optional)

CellHesion® NanoWizard® ForceRobot® NanoTracker™

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System options2


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NanoWizard® NanoOptics head in two versions: with fiber port for fiber SNOM experiments UV transparency for top-down illumination

The NanoWizard® NanoOptics AFM head is optimized for a broad range of applications ranging from nanoscale optical imaging by aperture and scattering-type SNOM to experiments involving interactions of light with the sample such as absorption, excitation, nonlinear effects and quenching. (see NanoWizard® NanoOptics brochure)

Key features

Comprehensive solutions for AFM and Raman spectroscopy, Tip-Enhanced Raman Spectroscopy (TERS), Aperture SNOM and Scattering-type SNOM (sSNOM), Confocal microscopy, NanoManipulation in optical fields Compatible with most commercially available

inverted research microscopes (Zeiss Axiovert and Axio Observer lines, Nikon TE and Ti lines, Olympus IX line and Leica DMI/DMi lines)

Unique integration with optical microscopy by tip and sample scanning design, DirectOverlay™ mode and smart engineering Seamless integration with inverted microscopes,

Raman spectrometers, photon counting systems 980 nm laser source for cantilever deflection de-

tection prevents cross talk with other wavelengths Wide range of operation modes and accessories such

as Tuning Fork, STM, Conductive AFM, Fiber SNOM

NanoWizard® ULTRA Speed for fast-scanning and super-resolution AFMThe NanoWizard® ULTRA Speed AFM head combines true atomic resolution and fast scanning with line rates up to 300Hz. (see NanoWizard® ULTRA Speed brochure)

Key features

Fast-scanning up to 300Hz line rate for tracking dynamic processes Comes with the easy-to-use QI™ mode for

quantitative material property mapping Atomic resolution in closed-loop mode by lowest

scanner, position-sensor and detection-system noise level

Unique integration with optical microscopy by tip-scanning design and DirectOverlay™ feature for most precise correlative microscopy Fully flexible design with the widest range of

modes and accessories

NanoWizard® 4 for high resolution and fast scanningThe new NanoWizard® 4 AFM head combines atomic resolution and fast-scanning possibility with line rates up to 70Hz in a system with a large scan range of 100 µm. (see NanoWizard® 4 brochure)

Key features

Tip-scanner technology for safe and easy operation in air, gases, and fluids Full transmission optical capabilities with standard

condenser; e.g. bright field, phase contrast, Hoffman modulation or DIC simultaneous to AFM imaging with inverted research microscopes Advanced optical techniques simultaneous to AFM

like FRET, TIRF, FLIM, FCS, single molecule detection, epi-fluorescence or confocal laser scanning (CLSM) with optional optical microscopes

Simultaneous operation with optical super- resolution techniques like STED, STORM/PALM and SIM JPK´s DirectOverlay™ feature for perfect

combination of AFM and optical microscopy Open hard and software architecture Optimum performance by active vibration

isolation table Comprehensive set of AFM modes like JPKs

easy-to-use QI™ mode, contact mode with lateral force, advanced AC modes like non-contact, phase detection, electrical and magnetic imaging modes

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TAO™ Tip Assisted Optics moduleSpecialized sample stage for advanced experiments combining AFM and optical spectroscopy based on proven NanoWizard® technology. 2 axis and 3 axis version available. (see TAO™ module product note)

Key features

Ultimate platform for TERS, scatter type SNOM, single molecule imaging/spectroscopy experiments NanoOptical studies such as quenching, field

enhancement or bleaching Integrated sample scanning confocal imaging Integrates with conventional and advanced optical

imaging (DIC, Phase contrast, CLSM, TIRF, FRET...) Flexible software system solution for experimental

freedom and remote operation

Ultimate flexibility through simultaneous control of up to 6 scan axes with closed loop precision (linearized with capacitive sensors): AFM tip scanner: 100 × 100 × 15 µm3 Sample scanner:

100 × 100 × 10 µm3

100 × 100 µm2 30 × 30 × 10 µm3 Fits to the Vortis™ Advanced controller

BioMaterials Workstation BioMAT™

Completely integrated system comprising upright optical microscope and AFM. The specialist for investigations of opaque samples in life and material sciences. (see BioMAT™ brochure)

Key features

Studies on non-transparent substrates or samples with AFM and optics Outstanding reproducibility of the focussed

position (ROI) with both systems Unique capability to investigate the ROI precisely

with optics and AFM Also perfect during operation in liquids

Wide range of applications: Biochips, cell chips or patterned substrates for cell adhesion, plant cell applications, microbiology, tissue engineering, ... Flexible concept with shuttle stage Both techniques can be operated even in different

laboratory rooms


CellHesion® 200The single cell force testing solution for cell adhesion and elasticity studies. Specialized platform for cell adhesion and cell mechanics measurements from single molecules to entire cells. (see CellHesion® 200 brochure)

Key features

Cantilever sensor lifting system with >110 µm travel range with closed-loop control through high-speed capacitive sensor feedback

Integrates with advanced optical imaging (DIC, CLSM, TIRF, FRET...) Fits to the Vortis™ controller

ForceRobot® 300The innovative force spectroscope with fully automated work flow. Take more than 80,000 force curves within 24 h while varying parameters such as temperature or loading rate. (see ForceRobot® 300 brochure)

Key features

Fully automated force spectroscope with highest flexibility Cutting edge force spectroscopy and force mapping

in combination with single molecule fluorescence Automated laser and detector alignment

Automated cantilever drift compensation Advanced and dedicated software with

ExperimentPlanner™, RampDesigner™ and built-in data batch processing Fits to the Vortis™ controller

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Software modules3


ExperimentControl™

Remote control and monitoring of complex and long-term experiments

Key features

Simplifies setting up the instrument especially inside an acoustic hood Continuous status update of the instrument

Tablet with holder is included

Remote control and monitoring of complex and long-term experiments via the internet by a PC, tablet or smartphone

Advanced AC modes moduleSoftware module for NanoWizard® systems

Key features

Comprehensive range of advanced dynamic feedback modes Phase Modulation (PM), Frequency Modulation

(FM) or self-excitation FM

Modes can be combined with Amplitude Gain Control (AGC) and Q-Control Higher harmonics with extra lock-in amplifier Requires cantilever holder with DirectDrive™ capability

ExperimentPlanner™ software moduleFor comprehensive planning of an experiment with all of the external parameters.

Key features

Delivers full control over all system options Design of advanced experiments

Scanners, motors, light sources, detectors, environmental control options etc can be master controlled and timed

QI™ Advanced software moduleFor NanoWizard® systems; delivers quantitative mechanical properties. (see QI™ brochure)

Key features

Any kind of sample can be imaged: samples with steep edges, loosely attached samples, soft, sticky and brittle samples Works under ambient conditions and in fluid Quick to learn and easy to operate Provides additionally adhesion, stiffness and

dissipation data while scanning

Depending on application, it can also deliver electrical conductivity or molecular recognition in a single scan Contact Point Imaging (CPI) mode for extremely

soft and inhomogeneous surfaces QI™ Advanced imaging mode for conductive measu-

rements in combination with the JPK CAFM module

CellHesion® moduleAs an add-on to the NanoWizard®, the CellHesion® module combines the capabilities of the BioAFM with precise adhesion force measurements and all optical microscopy features simultaneously. (see CellHesion® module product note)

Key features

100 µm additional z-travel Perfect hardware linearization through

capacitive sensors

Simultaneous focus tracking optional Integrates with advanced optical imaging

(DIC, Confocal microscopy, FRET...)

Applications modules and modes4

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Fast scanning packageFast scanning option for NanoWizard® 4

Key features

> 70 Hz line rate for scan ranges up to 2 µm Fast z-scanner and mechanical adaptation Ultrastable JPK DirectDrive™ cantilever excitation

for AC modes

>70 kHz z-scanner resonance frequency Software upgrade for fast scanning Supports FM-AFM and self-excitation FM-AFM Fast data acquisition


Advanced Force Spectroscopy moduleFor advanced force measurement experiments from single protein unfolding, DNA stretching to probing of cells and tissue.

Key features

Integrated RampDesigner™ for user defined segmentation of force curves

Advanced force clamp

Tuneable frequency sweep from 0.1 to 10 kHz Setting of user-defined patterns for rheology

applications

MicroRheology moduleFor visco-elastic properties of living cells and other samples such as gels or foams.

Key features

Extension to RampDesigner™ offering sine modulation segments Perform oscillating measurements with settings

for amplitude and frequency

Force history can be defined by the user through choice of preceding and following segments in RampDesigner™ Sine modulation analysis in the Data Processing

software fits amplitude and phase of response

350nm

NanoLithography/NanoManipulation moduleSoftware module for NanoWizard® systems.

Key features

Free-hand or vector-based patterns are possible Moving objects or scratching the substrate with

controlled force are possible

Pattern creation by electrical current such as anodic oxidation

DirectOverlay™ modulePerfect integration of optical and AFM data – JPK‘s proprietary and patented solution for perfect overlay of optical and AFM information.

Key features

Perfect overlay of optical and AFM data with sub-diffraction limit precision Direct “in optical image” selection of AFM

measurements (imaging and force curves) Dramatic reduction of overview image scanning in

AFM, giving faster results & lower tip contamination

Optical image navigation to specific regions of interest, even without AFM scanning. This protects functionalized tips for molecular recognition, avoiding tip passivation from image scanning before the force measurements.

Electrical sample connection module

Key features

Accommodates electrical conductive samples such as AFM metal stubs by magnetic fixation or transparent samples such as ITO coated glass coverslips Insert for Life Science stages

9

Conductive AFM moduleFor conductivity and I/V measurements on the nanoscale.

Key features

High-performance conductive AFM experiments Recording I/V curves Bias voltage range ± 10 V Noise limit 0.55 pA RMS

Sample holders for opaque samples Sample holder for transparent samples

works with all optical microscopes

CoverslipHolder with electrical sample connectionFor electrical measurements such as Conductive AFM or STM on a coverslip in combination with high NA optics.

Key features

Accommodates electrical conductive samples and transparent samples such as ITO coated glass coverslips Insert for Life Science stages

OEM micropipettesJPK offers a micropipette system, e.g. CellTram® models from Eppendorf, together with NanoWizard® or CellHesion® 200 systems.

Kelvin Probe (KPM) and Scanning Capacitance (SCM) Microscopy moduleOption for nanoscale mapping of surface potential distribution.

Key features

Surface Potential Mapping Electrical tip connection

External bias electronics module (+/-10 V) Software interface Enclosed volume version also available


HyperDrive™ fluid imaging package For highest resolution imaging of small structured samples in liquid. (see jpk website for more information)

Key features

Optional for Nanowizard® Nanoscience and Bioscience version Minimized tip-sample interaction by using smallest amplitudes Consists of a special cantilever holder with direct drive functionality, and a control software module

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High Voltage Sample Bias AmplifierCan be used for biasing a sample, e.g., in electro-optical experiments or in Piezo-Response Force Microscopy (PFM).

Key features

+/- 100 V can be applied between sample and tip Software controlled

Tuning Fork moduleThe Tuning Fork module fits directly to the NanoWizard® head.

Key features

Works with self excitation or external mechanical dither Allows vertical (shear force) or horizontal

mounting of tuning forks

Includes two holder boards and two unpackaged 40 kHz tuning forks for self assembly

Scanning Tunnelling Microscopy (STM) moduleThe STM module fits directly to the NanoWizard® head.

Key features

The module can accommodate different STM probes Is compatible to the JPK electrical sample

connection module

Compatible with the JPK TopViewOptics™ for controlled tip positioning

Tunneling Current Conductive AFM (TC-CAFM) moduleFor low-conducting samples.

Key features

Highest-performance, lowest current conductive AFM experiments Records local I/V curves Bias voltage range ± 10 V Current range with a gain 1*109 up to ± 10 nA

with a noise limit 100fA RMS in imaging bandwidth Other current ranges on request

(see JPK Conductive AFM module product note)

Optional version with closed sample volume for experiments in defined atmosphere with a volume of 140 µL In combination with QI™ Advanced mode and

NanoWizard® mechanical and electrical data can be correlated on the nanoscale in a single scan With QI™ Advanced high resolution and low noise,

current mapping on fragile, brittle and loosely attached samples with virtually no tip wear due to eliminated lateral forces


Conductive AFM module – Enclosed VolumeFor experiments in inert atmosphere; for conductivity and I/V measurements on the nanoscale under controlled environmental conditions.

Key features

High-performance conductive AFM experiments under user defined gas atmosphere Recording I/V curves Bias voltage range ± 10 V

Noise limit 0.55 pA RMS Sample holders for opaque samples Sample holder for transparent samples

works with all optical microscopes

11

ForceWheel™JPK‘s ForceWheel™ handheld accessory for most sensitive experiment control, e.g., for force spectroscopy.

Key features

Connected via USB Manual force curve acquisition

Continuous setting of gains and setpoints

Easy attachment of cells or beads with cantilever tip

Motorized precision stageOffers automatic motion control for precise positioning of the sample relative to optical axis and AFM probe.

Key features

Transmission illumination capability for inverted optical microscopes like Olympus IX line Zeiss Axio Observer/Axiovert 100/135/200 Nikon TE 2000 or Ti series Leica DMI/DMi lines Please specify model upon order Travel range of the sample 20 × 20 mm² Step size (Resolution): ≤ 1 µm Repeatability (unidirectional): ≤ 2 µm

Maximum velocity: 1 mm/s With joystick or software control For automated tiling or mapping applications Manual precision positioning of the AFM tip Stand alone use or for inverted optical microscopes Compatible with all JPK add-ons, glass slides,

Petri dishes etc. Compatible with NanoWizard®, ForceRobot® 300

and CellHesion® 200 heads

Sample stages5

HybridStage™Modular piezo based sample scanner stage combined with motorized coarse XY sample movement. With the HybridStage™, accurate and highly reproducible Z-stacks, important for image reconstruction, can be performed while the AFM stays in place.

Key features

Combined piezo and motorized stages for automated mapping or scanning of large areas Maps large objects such as cell scaffolds, printed

3D structures, micro-spheres or capsules and micro/ nano structured surfaces over millimeter distances Cell/cell or cell/substrate adhesion in 3D with a

large pulling range up to 200 x 200 x 200 µm³

Mechanical properties of plant cells, cell layers, cartilage, parasites, scaffolds, bones or tissue typical with rough surfaces over a mm range Optional XY or XYZ piezo flexure scanners with a

large travel range (depending on the application) can be implemented Automated and streamlined workflow with the

motorized HybridStage™

JPK 8x FluidicsModule™

Key features

JPK FluidicsModule™ with up to 8 different liquids such as buffer solutions Software-controlled flow rate

Automated incubation or adding of chemicals Comprehensive safety package


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Cantilever holder options6

Sample holder for large samplesThe holder module fits directly to the manual or motorized precision stage.

Key features

The holder can accommodate larger samples such as microchips or wafers and is equipped with spring clips which can be varied

Standard stageOffers fine motion control for precise positioning of the AFM tip relative to the sample.

Key features

Travel range of the AFM head (tip positioning) 10 × 10 mm² Stand alone use in combination

with JPK TopViewOptics™ Magnetic sample holder

Flexible design for fluid cells and different sample holders Rigid, chemical resistive surface Maximum flexibility for customized sample

mounting

Manual precision stageOffers sub-micron resolution & fine motion control for precise positioning of the AFM tip relative to optical axis and the sample.

Key features

Transmission illumination capability for inverted optical microscopes like Olympus IX line Zeiss Axio Observer/Axiovert 100/135/200 Nikon TE 2000 or Ti series Leica DMI/DMi lines Please specify model upon order Travel range of the sample 20 × 20 mm²

Precise positioning of the sample holder with thermally decoupled, magnetic fixation

Flexible design for fluid cells, temperature control options, and custom sample holders Detachable from optical microscope for stand alone

operation Drift compensated design Compatible with NanoWizard®, ForceRobot® 300

and CellHesion® 200 heads

Fixed-spring cantilever holderThe standard holder for all-round applications.

Key features

For air or aqueous solutions Compatible with JPK BioCell™, CoverslipHolder™

or PetriDishHeater ™ for easier navigation Robust design using glass and medical steel

Measurements in fluid droplets or in fluid baths like Petri dishes or home made fluid cells Immersible to 8 mm fluid depth Easy to clean Easy handling even with gloves


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Magnetic field cantilever holderThis holder is designed for magnetic cantilever actuation.

Side-view cantilever holder This holder is designed for use in combination with inverted optical microscopes to observe the cantilever region from the side.

Key features

For use with JPK BioCell™, CoverslipHolder™ or PetriDishHeater ™ for easier navigation Measurements in fluid droplets or in fluid baths like

Petri dishes or home made fluid cells

Immersible to 8 mm fluid depth Easy to clean Easy handling, even with gloves

Cantilever holder with electrical tip connectionThe holder is designed for use in air or liquids with a fixed cantilever spring and an electrical tip connection.

Key features

For air or aqueous solutions

Compatible with JPK BioCell™, CoverslipHolder™ or PetriDishHeater ™ for easier navigation Measurements in fluid droplets or in fluid baths

like Petri dishes or home made fluid cells

Robust design using glass and medical steel Immersible to 8 mm fluid depth Easy to clean Electrical tip connection

Supercut version cantilever holderThe holder is designed for use in air or liquids with removable cantilever spring for maximum cleaning results.

Key features

Chemically inert cantilever holder For use with JPK BioCell™, CoverslipHolder™ or

PetriDishHeater ™ for easier navigation Robust design using glass and medical steel or gold

spring only Immersible to 6 mm fluid depth

Easy to clean by detergents, ultrasonic bath or autoclaving Easy handling, even with gloves Measurements in droplets or in fluid baths like

Petri dishes or home made fluid cells Works in air and liquid, including aggressive media

Standard cantilever holderFor all-round applications with a large optical field of view.

Key features

Chemically inert cantilever holder Optimized for the use with top-view optics Robust design using glass and medical

steel or gold spring only Immersible to 6 mm fluid depth

Easy to clean by detergents, ultrasonic bath or autoclaving Easy handling, even with gloves Measurements in droplets or in fluid baths like

Petri dishes or home made fluid cells Works in air and liquid, including aggressive media


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CoverslipHolderWith the same design as the BioCell™ but without temperature control, the CoverslipHolder is the ultimate high resolution liquid cell with coverslip bottom.

Key features

Insert for Life Science stage Closed liquid cell with standard coverslip bottom High-N.A. immersion optics possible by exchange-

able cover slip bottom (0.17 mm thickness) Ports for perfusion & gas flow, e.g., CO2 or nitrogen

Superior design for combining AFM and confocal laser scanning Made of glass, steel or gold, and silicon only Easy to clean, also in ultrasonic bath Autoclavable inlet

PetriDishHeater™

The heater system is fully compatible with JPK‘s range of NanoWizard® AFM and CellHesion® systems. This means all the standard imaging and measurement techniques may be applied at temperatures up to 60 ˚C with 0.1 ˚C precision. These include fluorescence, brightfield, phase contrast and DIC, AFM imaging and cell mechanics study. The system also offers per-fusion control employing standard syringe pumps and gas flow of carbon dioxide possible for long term cell studies. Glass bottomed dishes are important for high numerical aperture fluorescence experiments such as confocal laser scanning used in combination with AFM.

Key features

Insert for Life Science stage Temperature range: room temperature up to 60°C Compatible with 35 × 10 mm Petri dishes from WPI

and TPP - plastic or plastic with glass bottom Adaptors for other suppliers such as Matek, Willco,

IWAKI, Ibidi, and BD upon request

Ports for perfusion tubes and gas supply (only available for certain types of Petri dishes) Software control Temperature stability 0.1 K Fully compatible with JPK‘s range of NanoWizard®

AFM and CellHesion® systems Fits to all inverted microscope brands in

combination with a JPK sample stage

Environmental control options – fluid cells and temperature controls7

BioCell™

The BioCell™ allows for the temperature-dependent study of living cells or single molecules with the NanoWizard® AFM, CellHesion® 200 and ForceRobot® 300 in their native environment. It is therefore particularly suited for applications in cell biology and biomedical research. As an add-on for inverted optical microscopes the NanoWizard® AFM in combination with the BioCell™ offers the unique possibility to simultaneously study temperature-dependent processes like cell motility and meta- bolism with the AFM and simultaneous advanced optical imaging. During AFM measurement all the optical microscope‘s operation modes, in particular contrast techniques, remain constantly available. Even high-resolution fluorescence confocal microscopy and single-molecule fluorescence techniques are possible since objective lenses with highest numerical aperture can be used.

Key features

Insert for Life Science stage Temperature range: 15 to 60°C Resolution: 0.1°C Quick-exchange cover slip bottom

Connections for liquid perfusion and gas flow (e.g., CO2 mix) Fully compatible with JPK‘s range of NanoWizard®

AFM, CellHesion® and ForceRobot® systems Drift-minimized in all direction


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OEM Humidifier from LISTogether with the BioCell™ and the PetriDishHeater™, JPK offers an external humidifier from LIFE IMAGING SERVICE (LIS), consisting of:

Key features

Digital gas flow sensor (< 3 bar in; < 1 bar out) Tubing (4 × 1 mm, 6 m) Humidity sensor Humidity sensor capsule

(from JPK, with 2 screws M 2.5×6 mm)

Humidity controller Set of tubings

Insulating tubing (OD/ID - 4×2mm) Transfer tubing to fluid chamber

SmallCell™ for harsh environmentsThe perfect solution for smallest volume experiments in a hermetically sealed environ-ment, the SmallCell™ is perfect for fluid or gas exchange. The SmallCell™ is compatible with the JPK temperature control systems like the HTHS™, HCM™ and the HCS™.

Key features

Solvent, acid, chloroform and acetone resistant Multi-purpose closed fluid cell for small

volumes (< 150 µl) Inlet and outlet ports for perfusion experiments

Glass, steel or gold, and resistive seal only Easy to clean, also in ultrasonic bath Autoclavable Temperature range up to 100 °C

PetriDishHeater™ for BioMAT™

Shuttle stage with integrated Petri dish heater for JPK BioMaterials workstation.

PetriDishHolder™Designed like the PetriDishHeater™, but without temperature control, the PetriDishHolder™ is the ultimate liquid cell for high resolution experiments in a Petri dish.

Key features

Temperature range: room temperature up to 60°C Compatible with 35 × 10 mm Petri dishes from WPI

and TPP – plastic or plastic with glass bottom

Dipping lenses need to be compatible with type of Petri dish Temperature stability 0.1 K Software control

Key features

Insert for Life Science stage Compatible with 35x10mm Petri dishes from WPI

and TPP (plastic or plastic with glass bottom) Adaptors for other suppliers such as Matek, Willco,

IWAKI, Ibidi, and BC upon request

Ports for perfusion tubes and gas supply (only available for certain types of Petri dishes) Openings for immersion medium application Silicone anti-evaporation seal


© LIFE IMAGING SERVICES

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Heating Cooling Module (HCM™)Designed for heating and cooling experiments in gas or liquids with minimized drift in all dimensions.

Key features

Temperature range from -30°C to 120°C ~0-80°C temperature range for experiments

in aqueous solutions Temperature resolution 0.1 °C

Compatible with JPK Manual precision stage and Motorized precision stage No transmission illumination possible

Heating Cooling Stage (HCS™)Designed for AFM experiments in air or liquids from 0 °C up to 100 °C with minimized drift in all dimensions – HCS™ follows the same design criteria as HTHS™.

Key features

Temperature range from 0 °C to 100 °C Temperature resolution 0.1 °C Twin stage thermoelectric element Drift minimized in all directions for full

temperature ramps simultaneous to AFM imaging

Heatpipe design – no water cooling or fan required Can be used together with closed fluid cells such

as SmallCell™

3-port small volume SmallCell™

The perfect solution for smallest volume experiments in a hermetically sealed environment, the small volume SmallCell™ is perfect for aqueous solutions exchange. The SmallCell™ is compatible with the JPK temperature control systems like the HTHS™, HCM™ and the HCS™.

Key features

Closed fluid cell for minimized volumes (< 60 µl) Optimized for aqueous solutions Inlet and outlet ports for perfusion experiments

with Luer connectors Temperature range up to 80 °C

Extra port for small amounts of liquids such as drugs, proteins etc. Polycarbonate, steel or gold, and seal only Easy to clean, also in ultrasonic bath Autoclavable

High Temperature Heating Stage (HTHS™)This high performance heating stage is designed for demanding polymer applications as well as for stable long time temperature studies of single molecules or nanoparticles.

Key features

Temperature range from ambient to 300°C in air and ambient to 80°C in fluid when used together with the JPK SmallCell™ Temperature resolution 0.1 °C

Drift minimized in all directions for full temperature ramps simultaneously with AFM imaging No transmission illumination possible


17

CryostageJPK offers specialized cryostages together with NanoWizard® systems for cooling applications up to -120°C. Please ask the JPK applications team for more information.

Electrochemistry solutions8

Temperature controlled ECCell™ Electrochemistry with temperature control and transmission optics such as phase contrast, DIC or fluorescence together with AFM. The unique combination of temperature controlled electrochemical measurements with high-end optics makes the ECCell™ the ideal tool for combined studies of temperature and potential sensitive biomolecules.

Key features

Works with transparent or non-transparent substrates Accommodates standard coverslips,

metal or silicon chips or other substrates Temperature range from ambient to 60 °C 500 µl liquid volume with perfusion capability Sealed design for inert gas filling

Wire electrodes and miniature reference electrode Suitable for conductive films or substrates

(e.g., ITO coated glass) Compatible with common potentiostats Allows the use of high numerical aperture lenses

for optimum fluorescence performance Unrestricted high resolution AFM imaging

Heating Cooling Stage (HCS™) with electrochemistry cellDesigned for electrochemistry AFM experiments with controlled heating and cooling of the sample.

Key features

For opaque samples Temperature range from 0°C to 100°C Temperature resolution 0.1°C Twin stage thermoelectric element Drift minimized in all directions for full

temperature ramps simultaneous to AFM imaging

Heatpipe design – no water cooling or fan required Top-view optical access for easy navigation 500 µl liquid volume with perfusion capability Sealed design for inert gas filling Wire electrodes and miniature reference electrode

OEM Potentiostats from IPSJPK offers third party potentiostats for electrochemistry applications together with NanoWizard systems.

© IPS GmbH & Co. KG


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JPK acoustic enclosureApproved for high performance applications: acoustic hood from JPK for utmost stability and isolation.

Key features

Bio-acoustic enclosure for S1 level applications, S2 lab compatibility possible on demand Divisible in two parts for easy transport and setup Special wall design made by sandwich construction

and acoustic foam for best damping results

2 front doors with windows Dimensions: 1100 mm × 1100 mm × 1250 mm Demands table with 1100 mm × 1100 mm area

JPK base for acoustic enclosureApproved for high performance applications: base frame with top plate from JPK for utmost stability.

Key features

Welded steel base frame

100 mm MDF sandwich table top Dimensions: footprint 1100 mm × 1100 mm Height adjustable

Vibration and acoustic isolation9

Active vibration isolation from Accurion

Key features

i4 Series – compact table-top vibration isolation unit

Active Workstation 780 and 900 – active vibration isolation workstations

© Accurion GmbH

Active vibration isolation from Table Stable

Key features

TS 150 Small size table-top vibration isolation system for loads up to 150kg

TS 140 medium size table-top system for loads up to 140kg

© Table Stable Ltd.

Active air damping tables from TMC

Key features

For large setups such as AFM/Raman combinations or confocal microscopy

For less-critical vibration level environments

© Technical Manufacturing Corporation


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Optics accessories10

TopViewOptics™ for use with inverted optical microscopes or the JPK BioMAT™

The optical system for non transparent samples or substrates such as HOPG,mica, ceramics, implants etc. Can be used in combination with inverted microscopes.

Key features

For opaque samples Long working distance zoom lens from Navitar™ 2 mm - 400 µm field of view

Köhler illumination, fiber light illumination Color CCD camera Easy positioning and focus adjustment

Raman reflector kitThe optical system for Tip-Enhanced Raman Scattering (upright-TERS) on opaque samples.

Key features

For opaque samples Compatible with common inverted microscope

Raman spectrometer setups

Fits to the JPK Raman reflector kit sample holder and JPK TAO™ modules

TopViewOptics™ with boom standThe optical system for non transparent samples or substrates such as HOPG, mica, ceramics, implants etc. Can be used in combination with inverted microscopes.

Key features



TopViewOptics™

The optical system for non transparent samples or substrates such as HOPG, mica, ceramics, metall etc.

Key features




20

OEM solutions and small parts11

JPK sample holder for small samplesAdaptor for standard sample holder. Holds magnetically fixed AFM metal stubs.

Stand for AFM headParking stand for all NanoWizard®, CellHesion® or ForceRobot® 300 heads.

Inverted optical microscope configurationsResearch lines of inverted optical microscopes from Zeiss, Olympus, Nikon, and Leica. Ask the JPK applications team for more information.

Upright Fluorescence Microscope (UFM) KitEnables the combined use of AFM and upright fluorescence zoom microscopy for co-localization experiments on opaque substrates.

Fiber coupled detection moduleAllows the precise capture of light emitted by the SPM tip region into a fiber coupled detector. Suitable for home-build SNOM or other light emitting tip experiments.

Key features

Fiber coupler for 50 µm core multi-mode optical fiber (FC connector) Fits to c-mount equipped inverted optical microscopes Precise manual xy positioning of the fiber end

Built-in CCD-camera system for the alignment of emission spot to fiber center Three way optical switch for viewing sample, fiber

end, and semi-transparent overlay


21

JPK cable anchorage pillowHeavy weight pillow for fixation of cables to reduce noise coupling.

JPK cantilever changing toolProbe loading station for convenient cantilever exchange.

JPK biocompatible glueFor cantilever or sample fixation, easy removable and biocompatible.

OEM syringe pumps JPK offers syringe pumps from several manufacturers.

Aladdin AL-1000 from WPI

Single pump for 1 - 60 mL or 0.5 - 5 µL syringes Step motor with 400 steps per revolution and 5.1 cm/min (max speed)/0.0042 cm/hr (min speed) Pumping rate 1699 mL/hr with 60 mL syringe/0.73 µL/hr with 1 mL syringe

KDS200 from KD Scientific

Two-syringe pump for sizes 10 µL to 140 mL 0.165 µm (1/16 step) advance per microstep Accuracy ± < 1%

JPK spare partsAvailable on request.

Springs for different cantilever holders Fluid cell seals Adapters

Coverslips Tubing for fluid cells


© World Precision Instruments Ltd.

© KD Scientific

22

AFM cantilevers, substrates and standardsThe NanoWizard® AFM platform is compatible with all commercially available cantilevers. JPK provides cantilevers from leading suppliers like AppNano, Budgetsensors, Mikromasch, NanoSensors, Nanotools, Nanocraft, NanoWorld, Team Nanotec, etc.

AFM Cantilevers

Standard contact and AC mode cantilevers High-resolution EBD tip cantilevers Chemically functionalized cantilevers Coated cantilevers

Tipless cantilevers for customized force experiments Test grids and substrates And many more options

Please visit our online store at cantilevers.jpk.com for an overview.

© NanoWorld AG, nanotools GmbH


CCD, EMCCD and CMOS camerasThe DirectOverlay™ optical integration can be used with any camera. The following CCD cameras are preferred and can be offered directly by JPK.

Andor® Technology

iXon+ EMCCD Luca EMCCD Clara Interline CCD Zyla sCMOS

© Andor Technology plc.

Jenoptik ProgRes® cameras series, such as

MFcool CFcool

© Jenoptik AG

The Imaging Source

FireWire CCD color camera

© Imaging Source Europe GmbH

23

Please see dedicated brochures or product notes for more detailed information on our website at www.jpk.com.

IDS Imaging Development Systems

Compact USB CCD and CMOS cameras of type µEye®

© IDS Imaging Development Systems GmbH

Photometrics

Evolve ™ EMCCD

© Photometrics

Hamamatsu Photonics K.K.

ORCA CMOS

© Hamamatsu Photonics K.K.


OEM Scanning Thermal AFM from Anasys or AppNano

JPK offers third party solutions for scanning thermal applications together with NanoWizard® systems.

OEM indentation solutionsJPK offers different third party solutions for indentation.

OEM incubators for Live Cell experiments under controlled environmentsJPK offers different incubator models for LIVE CELL applications together with NanoWizard® or CellHesion® systems.

OEM materials testing modules for mechanical propertiesJPK offers different third party modules for in-situ AFM experiments under tensile, compression, bending or torsion load.

OEM glove box systems for NanoWizard® AFMsThe AFM system can be used in a glove box under controlled atmosphere.

© GS Glovebox Systemtechnik GmbH

© M. Braun Inertgas-Systeme GmbH

© Anasys Instruments Corporation

© Applied NanoStructures, Inc.

JPK Instruments AG Colditzstraße 34-36 12099 Berlin, Germany tel.: +49 30 726243 500 fax.: +49 30 726243 999

www.jpk.com

NanoWizard, CellHesion, TAO, BioMAT, NanoTracker, ForceRobot, Vortis, DirectOverlay, QI, HyperDrive, RampDesigner, ExperimentPlanner, ExperimentControl, ForceWatch, ForceWheel, FluidicsModule, HybridStage, BioCell, SmallCell, ECCell, HTHS, HCS, HCM, TopViewOptics, PetriDishHeater are trademarks or registered trademarks of JPK Instruments AG. All information and specifications are subject to change without notice. All rights reserved. © 2015, JPK Instruments AG.

The handbook reflects a large variety of options and will grow further with latest developments. If you cannot find an accessory or operating mode in this handbook ask us directly. JPK is able to deliver customized solutions even for advanced applications.

BERLIN

HEADQUARTERS

UK

USA

SINGAPORE

JAPAN

FRANCE

Providing the best service and support worldwide is an essen-tial part of JPK‘s philosophy. To accelerate this, JPK founded global offices in Singapore, Tokyo, Shanghai, Cambridge (UK), Paris (France) and Carpinteria (USA). JPK has the largest appli-cations and service team specialized in life science and nano science applications to support your research.

Our representatives in different countries are hand-selected to offer a service which meets the JPK quality standards. We achieve this through long-term partnership and by continuous training of applications scientists and service engineers. For more detailed questions our JPK applications team is just an email or phone call away from you.

JPk WorLdWideJPK WORLDWIDE

JPK offers regular user trainings and courses for beginners‚ or experts in our imaging facilities worldwide. Here users can learn to operate new instruments or get tips and tricks ranging from experimental procedures to sample preparation.

To find a representative in your region visit our website or call us.

SHANGHAI

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USA China Japan Europe & other regions

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