Josip Juraj Strossmayer University of Osijek · Josip Juraj Strossmayer University of Osijek I Sveučilište Josipa Jurja Strossmayera u Osijeku Department of Biology (University

Josip Juraj Strossmayer University of Osijek I Sveučilište Josipa Jurja Strossmayera u Osijeku

Department of Biology (University of Osijek) I Odjel za biologiju Sveučilišta u Osijeku

Department of Chemistry (University of Osijek) I Odjel za kemiju Sveučilišta u Osijeku

Department of Physics (University of Osijek) I Odjel za fiziku Sveučilišta u Osijeku

Faculty of Food Technology Osijek (University

of Osijek) I

Prehrambeno-tehnološki fakultet Sveučilišta

u Osijeku

Faculty of Medicine (University of Osijek) I Medicinski fakultet Sveučilišta u Osijeku

Faculty of Agrobiotechnical Sciences Osijek

(University of Osijek) I

Fakultet agrobiotehničkih znanosti Sveučilišta

u Osijeku

Young Scientists Conference

May 23rd and 24th 2019, Osijek, Croatia

BOOK OF ABSTRACTS

Skup mladih znanstvenika

23. i 24. svibnja 2019. godine, Osijek, Hrvatska

KNJIGA SAŽETAKA

ISBN 978 - 953 - 7005 - 60 - 3

BOOK OF ABSTRACTS 2nd Young Scientists Days – Young Scientists Conference, Osijek

KNJIGA SAŽETAKA 2. Dani mladih istraživača – Skup mladih znanstvenika u Osijeku

Organizing Committee

Organizacijski odbor

Josip Juraj Strossmayer University of Osijek

Department of Biology

Department of Chemistry

Tanja Žuna Pfeiffer (Department of Biology) – President

Jelena Bijelić (Department of Chemistry) – President

Anamarija Stanković (Department of Chemistry)

Silvija Šafranko (Faculty of Food Technology Osijek)

Tatjana Šafarik (Department of Chemistry)

Igor Đerđ (Department of Chemistry)

Ivna Štolfa Čamagajevac (Department of Biology)

Mirna Velki (Department of Biology)

Dubravka Špoljarić Maronić (Department of Biology)

Anita Galir Balkić (Department of Biology)

Stela Jokić (Faculty of Food Technology Osijek)

Kristina Selthofer-Relatić (Faculty of Medicine Osijek)

Zvonko Antunović (Faculty of Agrobiotechnical Sciences

Osijek)

Igor Lukačević (Department of Physics Osijek)

Scientific Committee

Znanstveni odbor

Martina Medvidović-Kosanović

(Department of Chemistry)

Martina Šrajer Gajdošik (Department of

Chemistry)

Tomislav Balić (Department of Chemistry)

Elvira Kovač-Andrić (Department of

Chemistry)

Rosemary Vuković (Department of

Biology)

Zorana Katanić (Department of Biology)

Mirta Sudarić Bogojević (Department of

Biology)

Lidija Begović (Department of Biology)

Honorary Committee

Počasni odbor

Vlado Guberac (Josip Juraj Strossmayer University of Osijek)

Dražan Kozak (Josip Juraj Strossmayer University of Osijek)

Sonja Vila (Josip Juraj Strossmayer University of Osijek)

Drago Šubarić (Josip Juraj Strossmayer University of Osijek)

Robert Raponja (Josip Juraj Strossmayer University of Osijek)

Ljiljana Krstin (Department of Biology)

Berislav Marković (Department of Chemistry)

Jurislav Babić (Faculty of Food Technology Osijek)

Krunoslav Zmaić (Faculty of Agrobiotechnical Sciences Osijek)

Vanja Radolić (Department of Physics Osijek)

Jure Mirat (Faculty of Medicine Osijek)

Published by / Izdavači

Josip Juraj Strossmayer University of Osijek

Department of Biology

Department of Chemistry

Faculty of Food Technology Osijek

Editors / Urednici

Anamarija Stanković, Silvija Šafranko, Ivna Štolfa

Čamagajevac, Tanja Žuna Pfeiffer, Tihomir Živić

Proofreading / Lektoriranje

Tihomir Živić

Design and Layout / Grafička priprema

Silvija Šafranko

CIP zapis dostupan u računalnom katalogu Gradske i sveučilišne knjižnice Osijek pod brojem 141119096.

Auspices and sponsors Pokrovitelji i sponzori

Under the high auspices of the

President of the Republic of

Croatia Kolinda Grabar -

Kitarović

Pod visokim pokroviteljstvom

Predsjednice RH Kolinde

Grabar ‐ Kitarović

Ministry of Science and

Education of the Republic of

Croatia

Ministarstvo znanosti i

obrazovanja Republike

Hrvatske

Josip Juraj Strossmayer

University of Osijek

Sveučilište Josipa Jurja

Strossmayera u Osijeku

Osijek-Baranja County Osječko-baranjska županija

City of Osijek Grad Osijek

Croatian Chemical Society Hrvatsko kemijsko društvo

(HKD)

Labena d.o.o., Croatia Labena d.o.o., Hrvatska

Inchoo Osijek Inchoo Osijek

Zagrebacka Banka UniCredit Zagrebačka banka UniCredit

Inspecto d.o.o., Croatia Inspecto d.o.o., Hrvatska

Pivovara Osijek d.o.o., Croatia Pivovara Osijek d.o.o.,

Hrvatska

Kutjevo d.d., Croatia Kutjevo d.d., Hrvatska

Ru-Ve d.o.o., Croatia Ru-Ve d.o.o., Hrvatska

Kefo d.o.o., Croatia Kefo d.o.o., Hrvatska

Biosistemi grupa d.o.o. Biosistemi grupa d.o.o.

Karolina d.o.o., Croatia Karolina d.o.o., Hrvatska

Slavonska polja, Croatia Slavonska polja, Hrvatska

Biroprint d.o.o., Croatia Biroprint d.o.o., Hrvatska

Gorea Plus d.o.o., Croatia Gorea Plus d.o.o., Croatia

Jamnica d.d., Croatia Jamnica d.d., Croatia

Croatian Society of Chemical

Engineers

Hrvatskog društva kemijskih

inženjera i tehnologa

Croatian Academy of Sciences

and Arts

Hrvatska akademija znanosti i

umjetnosti

All pieces of information in this BOOK OF ABSTRACTS are a sole responsibility of the Abstract

Authors. The Publishers shall not be responsible for any usage of the data appearing in this document.

Also, the Publishers shall not be liable for any errors found in the Authors’ works.

Sadržaj sažetaka u ovoj KNJIZI SAŽETAKA isključiva je odgovornost autora. Izdavač nije

odgovoran za uporabu podataka objavljenih u sažetcima, pogreške i sl.

Table of Contents

Conference Programme SECTION 1 – Invited Lectures

A. Széchenyi, T. Šafarik 2 Scientific Centre of Excellence for Personalized Health Care

Znanstveni centar izvrsnosti za personaliziranu brigu o zdravlju

S. Jokić 3 Research projects - opportunities for new jobs, modern equipment and

scientific achievements Znanstveni projekti-prilika za nova radna mjesta, suvremenu opremu i

znanstvena dostignuća

M. Kovač, M. Bulaić, B. Šarkanj, T. Kovač, A. Nevistić 4 Inspecto Ltd. as a partner in collaborative research of mycotoxin occurence

Inspecto d.o.o. kao partner u istraživanju pojavnosti mikotoksina

T. Bogdanović, S. Marić 5 Public Institution Kopački rit Nature Park 2012 - 2019. Good Practice Examples

and Models of Business Cooperation JU Park Prirode Kopački rit 2012.- 2019. Primjeri dobre prakse i modeli poslovne

suradnje

SECTION 2 – Biology T. Đerđ, V. Peršić, D. K. Hackenberger, B. K. Hackenberger 7

The Application of Low-Cost Sensor Systems in Lemna Toxicity Tests Primjena niskobudžetnih senzorskih sustava u Lemna-testu

N. Graovac, B. Bruvo Mađarić, A. Klobučar, N. Landeka, T. Žitko , E. Merdić, M. Kučinić

8 The Barcoding of the Croatian Mosquito Fauna

Barkodiranje faune komaraca Hrvatske

M. Kovačević, D. K. Hackenberger, Ž. Lončarić, B. K. Hackenberger 9 Efflux Pump Activity in Enchytraeidae

Aktivnost efluks-pumpe enhitreida

J. Ćosić, M. Dugić, R. Vuković, I. Štolfa Čamagajevac, A. Vuković, S. Šormaz, L. Zubaj, M. Matić, Z. Lončarić

10 The Differences in Biochemical Mechanisms of Cadmium Tolerance in Three

Soybean Cultivars Razlike u biokemijskim mehanizmima tolerancije na kadmij kod tri kultivara

soje

T. Mandir, M. Kovačević, M. Šimić, M. Grgošević, T. Žuna Pfeiffer, D. Špoljarić Maronić

11 Floral Diversity of a Sub-Pannonic Steppic Grassland in Bistrinci

Raznolikost flore subpanonskog travnjaka u Bistrincima

SECTION 3 – Medicine N. Kolobarić, M. Mihalj, A. Matić, A. Stupin1, L. Barić. Kralik, G. Kralik,

I. Drenjančević 12

The Consumption of the Omega-3-Enriched Eggs Reduces an Oxidative Stress in Granulocytes of the Healthy Male Participants

Konzumacija jaja obogaćenih omega-3 masnim kiselinama smanjuje oksidativni stres u granulocitima zdravih muških ispitanika

M. Pejakić, Ž. Perić Kačarević, M. Matijević 13 The Use of Injectable Biphasic Calcium Phosphate in a Guided Bone

Regeneration: A study Plan and Preliminary Results Uporaba injekcijskog bifaznog kalcijeva fosfata u vođenoj regeneraciji kosti:

plan studije i preliminarni rezultati

T. Berlančić, I. Miškulin 14 The Prevalence and Risk Factors for Peer Violence among Adolescents

Učestalost i rizični čimbenici za pojavu međuvršnjačkog nasilja u populaciji adolescenata

SECTION 4 – Physics I. Lukačević, M. Varga-Pajtler, M. Mužević, S. K. Gupta 15

The Prospects for an Experimental Realization of Two-Dimensional Aluminum Allotropes

Mogućnosti eksperimentalnog ostvarenja dvodimenzionalnog aluminija

SECTION 5 – Chemistry D. Goman, J. Bijelić, A. Stanković, M. Medvidović-Kosanović, P. Cop,

B. Smarsly, I. Đerđ 16

The Optimization of Hydrothermal Method for the Synthesis of Triple Perovskites with a Sr3Mn2(W/Te)O9 Structure Type

Optimizacija hidrotermalne metode za sintezu trostrukih perovskita tipa Sr3Mn2(W/Te)O9

M. Bjelić, T. Šafarik, A. Széchenyi 17 A Coprecipitation Synthesis and Modification of Magnetic Spinel Ferrite CoFe2O4

Nanoparticles with Gold Koprecipitacijska sinteza i modifikacija zlatom magnetskih spinelnih feritnih

nanočestica CoFe2O4

Ž. Kajinić, J. Vešligaj Turkalj, Ž. Romić, A. Stanković, M. Medvidović-Kosanović

18 Electrochemical Detection of As3+ Ions

Elektrokemijska detekcija iona As3+

M. Sekulić, J. Bijelić, A. Stanković, M. Medvidović-Kosanović, P. Cop, B. Smarsly, I. Đerđ

19 An Aqueous Sol-Gel Synthesis of Double and Triple Perovskites with an

A2Ni(Te/W)O6 and A3Co2TeO9 Structure Type Vodena sinteza sol-gel dvostrukih i trostrukih perovskita tipa A2Ni(Te/W)O6 i

A3Co2TeO9

S. Zorić, T. Balić 20 A Synthesis and Characterization of Cu(II), Fe(III), and Cr(III) Polynuclear

Carboxylate Complexes Sinteza i karakterizacija polinuklearnih karboksilatnih kompleksa bakra(II),

željeza(III) i kroma(III)

M. Lončarić, M. Molnar 21 A Synthesis of Coumarin Derivatives via Knoevenagel Condensation

in Green Solvents Sinteza derivata kumarina Knoevenagelovom kondenzacijom u

zelenim otapalima

SECTION 6 – Agriculture D. Iljkić, M. Rastija, V. Kovačević 22

The Advantages of Lime Application on Acid Soils Prednosti kalcizacije kiselih tala

M. Kovačić, Z. Puškadija, R. Büchler 23 The Influence of Selection on the Traits of the Carniolan Honey Bee (Apis

mellifera carnica) in Croatia Utjecaj selekcije na osobine sive medonosne pčele (Apis mellifera carnica) na

području Hrvatske

P. Lucić, V. Rozman 24 Plant Substances and Inert Dusts – Natural Insecticide Formulations

in the Storage Insects Control

Biljne supstance i inertna prašiva — prirodne formulacije insekticida u kontroli skladišnih kukaca

V. Zebec, Z. Semialjac, M. Marković, V. Tadić, D. Radić, D. Rastija 25

The Influence of Physical and Chemical Properties of Different Soil Types on the Optimal Soil Moisture for Tillage

Utjecaj fizikalnih i kemijskih svojstava različitih tipova tla na optimalno stanje vlažnosti za obradu

M. Ižaković, K. Jukić, T. Marček 26 The Impact of High-Voltage Electrical Discharge on Winter Wheat under Drought

Utjecaj visokonaponskog električnog pražnjenja na ozimu pšenicu u uvjetima suše

B. Ljuboja, Z. Kralik 27 The Increase of Selenium Content in Eggs by the Usage of Biofortified Maize in

Hen Mixtures Povećanje sadržaja selena u jajima korištenjem biofortificiranog kukuruza u

hrani kokoši

SECTION 7 – Food Technology I. Ivić, A. Pichler 28

The Influence of Processing Parameters on the Retention of Aroma Compounds during Wine Concentration by a Reverse Osmosis and Nanofiltration

Utjecaj procesnih parametara na zadržavanje spojeva arome tijekom koncentriranja vina reverznom osmozom i nanofiltracijom

A.-M. Cikoš, I. Jerković, B. Šarkanj, S. Jokić 29 The Identification of Headspace Compounds, Volatiles, Fatty Acid Content and

Antifungal Activity of the Extracts of Macroalga Codium bursa Identifikacija vršnih para, isparljivih komponenata, sastava masnih kiselina te

antifungalna aktivnost ekstrakata makroalge Codium bursa

T. Kovač, I. Borišev, A. Lončarić, C. N. Ezekiel, M. Sulyok, R. Krska, B. Šarkanj

30 The Interaction of Fullerenol C60(OH)24 Nanoparticles with the Aflatoxigenic

Foodborne Fungi Interakcija nanočestica fulerenola C60(OH)24 s aflatoksikogenom plijesni

prisutnom u hrani

CONFERENCE PROGRAMME

PROGRAM SKUPA

2nd Young Scientists Days – Young Scientists Conference

2. Dani mladih istraživača – Skup mladih znanstvenika

May 23rd and 24th 2019, Osijek, Croatia · 23. i 24. svibnja 2019., Osijek, Hrvatska

Conference Programme / Program Skupa

Thursday, 23rd May 2019 / Četvrtak, 23. svibnja 2019.

09:00 – 10:00 Registration of participants

Prijava i registracija sudionika

10:00 – 11:00 Opening ceremony

Svečano otvaranje

11:00 – 12:00

Oral presentations / Usmena izlaganja

Moderators / Moderatori:

Igor Đerđ, Kristina Selthofer-Relatić, Zvonko Antunović

11:00 – 11:15 The Consumption of the Omega-3-Enriched Eggs Reduces an Oxidative

Stress in Granulocytes of the Healthy Male Participants

Konzumacija jaja obogaćenih omega-3 masnim kiselinama smanjuje oksidativni

stres u granulocitima zdravih muških ispitanika

N. Kolobarić, M. Mihalj, A. Matić, A. Stupin, L. Barić, Z. Kralik, G. Kralik, I.

Drenjančević

11:15 – 11:30 The Prevalence and Risk Factors for Peer Violence among Adolescents

Učestalost i rizični čimbenici za pojavu međuvršnjačkog nasilja u populaciji

adolescenata

T. Berlančić, I. Miškulin

11:30 – 11:45 A Synthesis of Coumarin Derivatives via Knoevenagel Condensation

in Green Solvents

Sinteza derivata kumarina Knoevenagelovom kondenzacijom u zelenim

otapalima

M. Lončarić, M. Molnar

11:45 – 12:00 The Advantages of Lime Application on Acid Soils

Prednosti kalcizacije kiselih tala

D. Iljkić, M. Rastija, V. Kovačević

12:00 – 13:00 Lunch Break

Pauza za ručak

13:00 – 14:00



Dubravka Špoljarić Maronić, Martina Šrajer Gajdošik, Silvija Šafranko

13:00 – 13:15 The Increase of Selenium Content in Eggs by the Usage of Biofortified

Maize in Hen Mixtures

Povećanje sadržaja selena u jajima korištenjem biofortificiranog kukuruza u

hrani kokoši

B. Ljuboja, Z. Kralik

13:15 – 13:30 The Influence of Processing Parameters on the Retention of Aroma

Compounds during Wine Concentration by a Reverse Osmosis and

Nanofiltration

Utjecaj procesnih parametara na zadržavanje spojeva arome tijekom

koncentriranja vina reverznom osmozom i nanofiltracijom

I. Ivić, A. Pichler

13:30 – 13:45 The Application of Low-Cost Sensor Systems in Lemna Toxicity Tests

Primjena niskobudžetnih senzorskih sustava u Lemna-testu

T. Đerđ, V. Peršić, D. K. Hackenberger, B. K. Hackenberger

13:45 – 14:00 The Use of Injectable Biphasic Calcium Phosphate in a Guided Bone

Regeneration: A study Plan and Preliminary Results

Uporaba injekcijskog bifaznog kalcijeva fosfata u vođenoj regeneraciji kosti:

plan studije i preliminarni rezultati

M. Pejakić, Ž. Perić Kačarević, M. Matijević

14:00 – 14:30 Coffee Break

Pauza za kavu

14:30 – 15:30



Anamarija Stanković, Lidija Begović, Mirna Velki

14:30 – 14:45 Efflux Pump Activity in Enchytraeidae

Aktivnost efluks‐pumpe enhitreida

M. Kovačević, D. K. Hackenberger, Ž. Lončarić, B. K. Hackenberger

14:45 – 15:00 A Coprecipitation Synthesis and Modification of Magnetic Spinel Ferrite

CoFe2O4 Nanoparticles with Gold

Koprecipitacijska sinteza i modifikacija zlatom magnetskih spinelnih feritnih

nanočestica CoFe2O4

M. Bjelić, T. Šafarik, A. Széchenyi

15:00 – 15:15 The Influence of Selection on the Traits of the Carniolan Honey Bee

(Apis mellifera carnica) in Croatia

Utjecaj selekcije na osobine sive medonosne pčele (Apis mellifera carnica) na

području Hrvatske

M. Kovačić, Z. Puškadija, R. Büchler

15:15 – 15:30 The Interaction of Fullerenol C60(OH)24 Nanoparticles with the

Aflatoxigenic Foodborne Fungi

Interakcija nanočestica fulerenola C60(OH)24 s aflatoksikogenom plijesni

prisutnom u hrani

T. Kovač, I. Borišev, A. Lončarić, C. N. Ezekiel, M. Sulyok, R. Krska, B.

Šarkanj

15:30 – 15:45 Discussion and conclusions

Rasprava i zaključci

2nd Young Scientists Days – Young Scientists Conference

2. Dani mladih istraživača – Skup mladih znanstvenika

May 23rd and 24th 2019, Osijek, Croatia · 23. i 24. svibnja 2019., Osijek, Hrvatska

Conference Programme / Program Skupa

Friday, 24th May 2019 / Petak, 24. svibnja 2019.

08:00 – 09:00 Registration of participants

Prijava i registracija sudionika

09:00 – 10:00

Invited lectures / Pozvana predavanja


Tanja Žuna Pfeiffer, Anamarija Stanković, Tatjana Šafarik

09:00 – 09:20

Scientific Centre of Excellence for Personalized Health Care


A. Széchenyi, T. Šafarik

09:20 – 09:40

Research projects - opportunities for new jobs, modern equipment and

scientific achievements

Znanstveni projekti-prilika za nova radna mjesta, suvremenu opremu i


S. Jokić

09:40 – 09:50

Inspecto Ltd. as a partner in collaborative research of mycotoxin occurence


M. Kovač, M. Bulaić, B. Šarkanj, T. Kovač, A. Nevistić

09:50 – 10:00

Public Institution Kopački rit Nature Park 2012 - 2019. Good Practice

Examples and Models Of Business Cooperation

JU Park Prirode Kopački rit 2012.- 2019. Primjeri dobre prakse i modeli

poslovne suradnje

T. Bogdanović, S. Marić


Pauza za kavu

10:30 – 11:30



Stela Jokić, Ivna Štolfa Čamagajevac, Jelena Bijelić

10:30 – 10:45

The Identification of Headspace Compounds, Volatiles, Fatty Acid Content

and Antifungal Activity of the Extracts of Macroalga Codium bursa

Identifikacija vršnih para, isparljivih komponenata, sastava masnih kiselina te

antifungalna aktivnost ekstrakata makroalge Codium bursa

A.-M. Cikoš, I. Jerković, B. Šarkanj, S. Jokić

10:45 – 11:00 The Barcoding of the Croatian Mosquito Fauna


N. Graovac, B. Bruvo Mađarić, A. Klobučar, N. Landeka, T. Žitko , E. Merdić,

M. Kučinić

11:00 – 11:15 The Optimization of Hydrothermal Method for the Synthesis of Triple

Perovskites with a Sr3Mn2(W/Te)O9 Structure Type

Optimizacija hidrotermalne metode za sintezu trostrukih perovskita tipa

Sr3Mn2(W/Te)O9

D. Goman, J. Bijelić, A. Stanković, M. Medvidović-Kosanović, P. Cop, B.

Smarsly, I. Đerđ

11:15 – 11:30 Electrochemical Detection of As3+ Ions


Ž. Kajinić, J. Vešligaj Turkalj, Ž. Romić, A. Stanković, M. Medvidović-

Kosanović


Pauza za kavu

12:00 – 13:00



Anita Galir Balkić, Tatjana Šafarik, Martina Medvidović – Kosanović

12:00 – 12:15 Plant Substances and Inert Dusts – Natural Insecticide Formulations in the

Storage Insects Control

Biljne supstance i inertna prašiva — prirodne formulacije insekticida u kontroli

skladišnih kukaca

P. Lucić, V. Rozman

12:15 – 12:30 The Prospects for an Experimental Realization of Two-Dimensional

Aluminum Allotropes


I. Lukačević, M. Varga-Pajtler, M. Mužević, S. K. Gupta

12:30 – 12:45 An Aqueous Sol-Gel Synthesis of Double and Triple Perovskites with an

A2Ni(Te/W)O6 and A3Co2TeO9 Structure Type

Vodena sinteza sol-gel dvostrukih i trostrukih perovskita tipa A2Ni(Te/W)O6 i

A3Co2TeO9

M. Sekulić, J. Bijelić, A. Stanković, M. Medvidović-Kosanović, P. Cop, B.

Smarsly, I. Đerđ

12:45 – 13:00 The Influence of Physical and Chemical Properties of Different Soil Types

on the Optimal Soil Moisture for Tillage

Utjecaj fizikalnih i kemijskih svojstava različitih tipova tla na optimalno stanje

vlažnosti za obradu

V. Zebec, Z. Semialjac, M. Marković, V. Tadić, D. Radić, D. Rastija

13:00 – 14:00 Lunch Break

Pauza za ručak

14:00 – 15:00



Elvira Kovač-Andrić, Tanja Žuna Pfeiffer, Zorana Katanić

14:00 – 14:15 The Differences in Biochemical Mechanisms of Cadmium Tolerance in

Three Soybean Cultivars

Razlike u biokemijskim mehanizmima tolerancije na kadmij kod tri kultivara soje

J. Ćosić, M. Dugić, R. Vuković, I. Štolfa Čamagajevac, A. Vuković, S. Šormaz,

L. Zubaj, M. Matić, Z. Lončarić

14:15 – 14:30 A Synthesis and Characterization of Cu(II), Fe(III), and Cr(III)

Polynuclear Carboxylate Complexes

Sinteza i karakterizacija polinuklearnih karboksilatnih kompleksa bakra(II),

željeza(III) i kroma(III)

S. Zorić, T. Balić

14:30 – 14:45 The Impact of High-Voltage Electrical Discharge on Winter Wheat under

Drought

Utjecaj visokonaponskog električnog pražnjenja na ozimu pšenicu u uvjetima

suše

M. Ižaković, K. Jukić, T. Marček

14:45 – 15:00 Floral Diversity of a Sub-Pannonic Steppic Grassland in Bistrinci


T. Mandir, M. Kovačević, M. Šimić, M. Grgošević, T. Žuna Pfeiffer, D.

Špoljarić Maronić

15:00 – 15:30 Awards and Conference closing

Podjela nagrada i zatvaranje Skupa

19:00 – 21:00 Garden party – After-Conference Gathering (Department of Biology and Chemistry, Ulica cara Hadrijana 8/A, 31 000 Osijek)

INVITED LECTURES

POZVANA PREDAVANJA

2

INVITED LECTURE

Scientific Centre of Excellence for Personalized Health Care


A. Széchenyi*, T. Šafarik

Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Ulica cara Hadrijana 8/A, HR-31000 Osijek,

Croatia

*corresponding author: [email protected]

Abstract: Activities of Scientific Centre of

Excellence for Personalized Health Care

(SCEPersonHealth) are manly directed towards

connecting and developing cooperation of scientific

and educational institutions in order to integrate and encourage synergy, as well as promote international

competitiveness in the field. Capacity of SCEPersonHealth covers innovative research in the health and

pharmaceutical – biotechnological industry ranging from diagnosis and better understanding of the

disease through personalization and medical treatment. There is also conduction of fundamental and

clinical research in order to discover new insights into human genetics and molecular basis of the disease

to provide greater precision in diagnosis and targeted drug development. SCEPersonHealth is consisted

of two complementary units: 1) Glycemic Research and 2) Research, Production and Medical Functional

Food Testing. The main research problem of the first unit is understanding of the role of glycans in the

pathology of diseases associated with changes in glycosylation and development of high-velocity

glycemic and identification of prognostic markers. The second unit is developing new methods and

devices for quantification of antioxidant capacities, that are going to be used in clinical studies of the

functional food consumption that is going to be developed and produced within the activities of

SCEPersonHealth. Main focus of Department of Chemistry as a part of a second unit of

SCEPersonHealth, is development of new microsensor as a detector for monitoring and quantification

of antioxidant properties of biological materials. The results, new knowledge, equipment and

employment acquired as a part of the project directly contribute to the strengthening of the overall

research community, new technologies and ensuring higher product quality in the production of

functional food benefit food manufactures and processors through the production of high-quality

functional food that will enable them to strengthen market position. And also there is impact on long

term health improvement of general population.

Keywords: Functional food, Antioxidant capacity, Chemical sensor, Biosensor

mailto:[email protected]

3

INVITED LECTURE

Research projects - opportunities for new jobs, modern equipment, and

scientific achievements

Znanstveni projekti - prilika za nova radna mjesta, suvremenu opremu i


S. Jokić*

Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31000 Osijek,

Croatia


Abstract: Scientists at the Faculty of Food

Technology Osijek not only participate in higher

education, but also conduct their scientific research

through various national and international scientific

projects. Laboratories at the Faculty are well-

equipped with various modern analytical and pilot-

scale equipment used for the implementation of the

teaching process as well as for scientific research. The

realization of a number of international projects is

funded from various EU Programmes, the Croatian

Science Foundation, etc. Since 2015, the Faculty of

Food Technology Osijek has been a partner

institution in the Centre of Research Excellence for

Marine Bioprospecting – BioProCro, with the Ruđer

Bošković Institute as the host institution. The quality and quantity of scientific research can be seen in

numerous scientific papers that have been published in internationally acknowledged journals indexed

in various databases and presented at international conferences. The number of scientific papers with

foreign scientists as co-authors has been constantly increasing.

The Faculty has continuously been working on the connection and cooperation among research groups

within the Faculty, but also on conducting interdisciplinary research among different institutions.

A few selected projects will be presented in this presentation. Those types of projects provide plenty of

advantages for applicants and graduates, who can be hired on the project. It is also important to note

that research projects are providing funds for new modern research equipment as well. But the most

important thing is that research projects offer young researchers an opportunity to start their scientific

carrier, deal with scientific topics and methodologies, share and develop ideas, learn from each other,

and gain knowledge from senior researchers and mentors. Moreover, work on a project very often

includes enrolment in postgraduate studies, which is an opportunity to gain a PhD.

Scientific research, as well as mobility, at the Faculty of Food Technology Osijek contribute to the

achievement of national and international research excellence in various fields and to the transfer and

application of gained knowledge for the prosperity of society.

Keywords: research projects, science, opportunities, funding

4

INVITED LECTURE

Inspecto Ltd. as a partner in collaborative research of mycotoxin

occurence


M. Kovač1*, M. Bulaić1, B. Šarkanj2, T. Kovač3, A. Nevistić1

1 Inspecto d.o.o.., Vukovarska cesta 239b, Industrijska zona Nemetin, Osijek, Croatia 2 University North, Department of Food Technology, Trg dr. Žarka Dolinara 1, 48000 Koprivnica, Croatia

3Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31000 Osijek,

Croatia


Abstract: Inspecto d.o.o. is a specialized

inspection company with 20+ years of experience

in providing complete services in the control of

raw materials and finished goods in Croatia and

abroad, for which the company has been

authorized by the competent state institutions, thus

providing its partners additional quality of service,

security and impartiality. Accreditation by

Croatian Accreditation Agency according to the

requirements of the standard HRN EN ISO/IEC

17025 is particularly important in the field of

laboratory testing, as it represents a great

competence of the laboratory, enabled by hard

work of highly educated multidisciplinary team of

experts responsible for analytical procedures,

especially for the contaminants determination, as

well as the implementation of the state of the art techniques, such as chromatography techniques coupled

with tandem mass spectrometry (LC-MS/MS, GC-MS/MS) or inductively coupled plasma mass

spectrometry (ICP-MS).

Besides mentioned commercial activities, Inspecto Ltd. makes a lot of effort supporting of scientific

research, as being one of the partners of international collaborations, related to mycotoxin occurrence

and new strategies for reductions of fungal colonization and their harmful secondary metabolites. At

this moment, this is mainly related to aflatoxins reduction strategies by using new-synthesized

compounds or investigation of new abiotic stressors impact, such as fullerene and fullerol nanoparticles.

Furthermore, Inspecto Ltd. is engaged through postgraduate research of its employee in the development

and application of LC-MS/MS dilute and shoot multi-mycotoxin method, intended for faster, cheaper

and more comprehensive examination of (multi)mycotoxin contamination level in various food and feed

matrices, crucial for proper health risk assessment, and thus public health insurance.

Keywords: Inspecto, Croatia, collaboration, mycotoxins, contamination, LC-MS/MS


5

INVITED LECTURE

Public Institution Kopački rit Nature Park 2012 - 2019. Good Practice

Examples and Models of Business Cooperation

JU Park Prirode Kopački rit 2012.- 2019. Primjeri dobre prakse i modeli

poslovne suradnje

T. Bogdanović1,*, S. Marić2

1 Public Institution Kopački rit Nature Park, Mali Sakadaš 1, 31327 Kopačevo, Croatia 2 Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, Crkvena 21, HR-31000 Osijek,

Croatia

*corresponding author: [email protected] Abstract: Public Institution Kopački rit

Nature Park currently has 36 employees,

of which 16 employees are financed from

the state budget, 13 from own costs and 7

employees working on EU projects. Four persons were permanently hired in the period from 2012 to

2019. Among the given number of employees, 25 of them are permanently employed and 11 are

temporarily employed. Seasonal employees are hired according to our needs. Within the above

mentioned period, a total value of 23.500.000,00 HRK without VAT was realized through projects. The

implementation of the following EU projects is ongoing: “Presentation and Education Centre Tikveš”,

“Project of Construction of Sakadaš tourist dock in Nature Park Kopački rit" worth more than

60.000.000,00 HRK without VAT, project “Naturavita”, which is in the first phase of realization worth

about 48.000.000 EUR (Croatian Forest Management, Croatian Water Management, Public Institution

Kopački rit Nature Park), Public Institution Kopački rit Nature Park component about 4.000,00 HRK

and the Danubeparks project worth about 200.000,00 EUR. In 2016, compared to 2015, our own income

increased 37 %, whereas the number of visitors increased 30 %. In 2018. compared to 2017, the number

of visitors increased 7.2 % with a further increasing trend, whereas the income increased 2 %. Apart

from financial assets, the benefit from projects is also the possibility to hire employees under a

temporary contract or a permanent contract where the salaries are financed from own income, achieved

through the projects.

Keywords: Kopački rit, nature protection, EU project, business cooperation

ORAL PRESENTATIONS

USMENA IZLAGANJA

7

(Biology)

ORAL PRESENTATION

The Application of Low-Cost Sensor Systems in Lemna Toxicity Tests

Primjena niskobudžetnih senzorskih sustava u Lemna-testu

T. Đerđ1, V. Peršić1, D. K. Hackenberger2, B. K. Hackenberger1

1Josip Juraj Strossmayer University of Osijek, Department of Biology, Cara Hadrijana 8A, HR-31000 Osijek, Croatia 2BioQuant d.o.o., Našička 4, HR-31 000 Osijek, Croatia *corresponding author: [email protected]

Abstract: In plants, a relation between

respiration and photosynthesis reflects the

exchange of CO2 with the atmosphere. Until now,

only a few studies have measured a whole-plant

or a plant – community CO2 release/uptake

directly and continuously without disturbances.

Although the methods implemented in these

studies are of high accuracy, they are still cost‐ineffective, require complex experimental setups,

and provide discrete and discontinuous output

data. This work presents an application of real-time

continuous monitoring of the emission and uptake rate of CO2 in the Lemna toxicity test, implementing

a low-cost Arduino platform-based respiratory activity measuring system prototype (ResTox). The Lemna plants were cultured and maintained as described in the standard protocols. Each

experimental set consisted of six test chambers (including control) for each test compound and one

reference chamber. In each chamber containing a culturing medium, 21 fronds were placed. The plants

were exposed to the known concentrations of toxicants: metals and herbicides. The metal treatments

consisted of Hg, Cu, Cd and Co, while the herbicides chosen were diquat, tembotrione, nicosulfuron

and clopyralid. The concentration ranges were chosen based upon preliminary tests. The experimental

setup was kept in darkness for 3 hours, and during that time a respiratory activity was recorded. During

the next 3‐hour period, the lights were switched on and a CO2 assimilation was recorded. All

measurements were performed by an Arduino platform-based respiratory activity measuring system for

the real-time CO2 emission/uptake measurements, a prototype developed and assembled by the research

team. The measurements of CO2 concentrations were reported as dynamic curves. The results of CO2

measurements demonstrated a hormetic effect of examined substances: the low doses of metals and

herbicides stimulated a CO2 exchange rate, while at high doses they inhibited the CO2 exchange rate.

The approach presented in this work enabled an evaluation of acute toxicity mechanisms using a CO2

exchange flux rate as an endpoint during the phytotoxicity experiments.

Keywords: phytotoxicity, heavy metals, herbicides, CO2 sensors


8

(Biology)

ORAL PRESENTATION

The Barcoding of the Croatian Mosquito Fauna


N. Graovac1,*, B. Bruvo Mađarić2, A. Klobučar3, N. Landeka4, T. Žitko5 , E. Merdić1, M. Kučinić6

Josip Juraj Strossmayer University of Osijek, Department of Biology, Cara Hadrijana 8/A, HR-31000 Osijek, Croatia 2Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb, Croatia 3Andrija Štampar Teaching Institute of Public Health, Mirogojska 16, HR-10000 Zagreb, Croatia 4Institute of Public Health of the Istrian County, Nazorova 23, HR-52100 Pula, Croatia 5Education Institute of Public Health of Split‐Dalmatia County, Vukovarska 46, HR-21000 Split, Croatia 6Department of Biology, University of Zagreb, Roosveltov trg 6, HR-10000 Zagreb, Croatia


Abstract: Mosquitoes (Culicidae) represent a

frequently investigated group of insects within the

order (Diptera) precisely because they are human and

animal disease vectors. A mosquito identification is

therefore a key step in the development of the vector

control strategies. To achieve a more precise

identification, the morphological methods should be

complemented with the molecular identification

methods. A DNA barcoding is a global method of

biodiversity recognition, and the world’s base is being

continuously supplemented. Currently, the most

commonly used barcode region is the 50-segment one

of the mitochondrial gene Cytochrome Oxidase I

(COI). A DNA ‐ based identification makes it

possible to use the sequence data in order to determine

the species and their subspecies. Fifty-three species of

mosquitoes have been recorded in Croatia so far.

Determining the genetic status of this insect group will be of a great taxonomic, phylogenetic, and

phylogeographic importance since Croatia stands out for its diversity of habitats that affect genetic

diversity. During the DNA Barcoding of the Croatian Fauna project, mosquitoes were sampled from

the continental, alpine, and Mediterranean regions of Croatia in the mosquito seasons of 2017 and 2018.

Within the sampled mosquitoes, twenty-four species were determined and molecularly identified. By

the end of this project, we expect that we will have a DNA barcoding conducted on many species in

Croatia. We intend to find the cryptic and sibling species in the field (such as Anopheles maculipennis

complex and Culex pipiens complex) to resolve a genetic similarity. The results of this project will

provide more knowledge about genetic diversity in relation to a geographical distribution. Also, these

results will contribute to the complementation of the world’s DNA base (BOLD and NCBI).

Keywords: species, mosquito identification, DNA barcoding, genetic diversity, species complexes


9

(Biology)

ORAL PRESENTATION

Efflux Pump Activity in Enchytraeidae

Aktivnost efluks-pumpe enhitreida

M. Kovačević1,*, D. K. Hackenberger 1, Ž. Lončarić 1, B. K. Hackenberger 1

1 Josip Juraj Strossmayer University of Osijek, Department of Biology, Cara Hadrijana 8/A, HR-31000 Osijek, Croatia


Abstract: A multixenobiotic resistance mechanism

(MXR) is a cellular detoxification mechanism

present in all organisms, including microbes, plants,

invertebrates and vertebrates. It is mediated by the

activity of the ATP‐ binding cassette (ABC)

transporters. The MXR proteins (efflux pump)

transfer certain groups of xenobiotics out of the cell

and keep the intracellular concentration of these

compounds low.

The enchytraeids are the ecologically relevant soil

organisms, which play an important role in an

organic matter decomposition and soil bioturbation.

In the phylum Annelida MXR, an activity was

discovered mainly in the earthworm species, but

there were no enchytraeid species discovered.

For the identification of an efflux pump activity,

fluorescent substrates are often used. In case of an MXR system inhibition, the concentration of substrate

in the cells increases, and this can be seen as a rise in fluorescence.

Two enchytraeid species (Enchytraeus albidus and E. crypticus) were exposed to an ISO test water, with

or without specific chemosensitizers, at various concentrations. Subsequent to a pretreatment, they were

exposed to the fluorescent substrates, Rhodamine B (RhB) or Rhodamine 123 (Rh123). The amount of

RhB and Rh123 in the samples was measured using a spectrofluorometer.

Using the RhB and R123 as a fluorescent substrate, it could be seen that different substrates and

chemosensitizers exert an effect on the amount of fluorescence in the enchytraeids. The ability of

enchytraeids to remove the xenobiotics from the cells will greatly impact the final detrimental effects.

Determining a presence and function of the MXR in enchytraeids can help us understand the

consequences of MXR inhibition at the population and ecosystem level.

This study demonstrates that the enchytraeids, as a model species in the terrestrial ecotoxicology,

possess an MXR activity and that different substrates and chemosensitizers have an impact on it.

Therefore, it can be presumed that the xenobiotics present in the environment will have an effect on an

MXR system as well. Due to the influence of an MXR activity on the bioaccumulation and toxicity of

various compounds, the assessment of an MXR activity could be a valuable complementary biomarker

in ecotoxicological research and risk assessment.

Keywords: enchytraeids, chemosensitizers, MXR proteins, efflux pump, biomarker


10

(Biology)

ORAL PRESENTATION

The Differences in Biochemical Mechanisms of Cadmium Tolerance in

Three Soybean Cultivars

Razlike u biokemijskim mehanizmima tolerancije na kadmij kod tri

kultivara soje

J. Ćosić1*, M. Dugić1, R. Vuković1, I. Štolfa Čamagajevac1, A. Vuković1, S. Šormaz1, L. Zubaj1,

M. Matić2, Z. Lončarić2

1 Josip Juraj Strossmayer University of Osijek, Department of Biology, Cara Hadrijana 8/A, HR-31000 Osijek, Croatia

2 Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-

31000 Osijek, Croatia


7

8958

Abstract: An increased cadmium content in agricultural

soils causes changes in the physiological and biochemical

processes in plants, affecting their growth and yield.

Different plant species, but also the different cultivars of

the same species, widely vary in the terms of cadmium

accumulation and tolerance. In this respect, a production

of soybean (Glycine max L.) as a leading oil and protein

culture is endangered by a low cadmium tolerance.

Therefore, the aim of this study is to determine the

differences in the biochemical mechanisms of cadmium

tolerance in the leaves of three Croatian soybean cultivars

(Korana, Ika, and Lucija), which differ in the capacity of

cadmium accumulation. The soybean was hydroponically

cultivated under the controlled conditions and exposed to

cadmium (100 μmol L-1) for seven days. As the indicator

of an oxidative stress, a content of H2O2 and lipid

peroxidation levels, expressed in terms of the thiobarbituric acid’s reactive substances, was determined,

while an antioxidative status was determined by the activities of the antioxidative enzymes, such as the

catalase, ascorbate peroxidase, glutathione peroxidase and guaiacol peroxidase. A cadmium treatment

increased the lipid peroxidation levels in the leaves of all three soybean cultivars, indicating the presence

of an oxidative stress. Also, the activity of glutathione peroxidase was increased in all cadmium‐treated

plants, while other enzymes demonstrated different activity patterns in the investigated cultivars. The

cultivar Korana, which manifested the highest level of cadmium accumulation, also manifested the

highest level of tolerance with regard to two other cultivars. The most sensitive cultivar, Ika, with a

moderate accumulation capacity, manifested the lowest level of an antioxidative response. The

biochemical mechanisms of tolerance to a cadmium stress differed in the three investigated soybean

cultivars, and a tolerance to cadmium was partly achieved by an increased antioxidative response.

Keywords: antioxidative response, cadmium, oxidative stress, soybean, cultivars

11

(Biology)

ORAL PRESENTATION

Floral Diversity of a Sub-Pannonic Steppic Grassland in Bistrinci


T. Mandir*, M. Kovačević, M. Šimić, M. Grgošević, T. Žuna Pfeiffer, D. Špoljarić Maronić

Josip Juraj Strossmayer University of Osijek, Department of Biology, Cara Hadrijana 8/A, HR-31000 Osijek, Croatia


Abstract: A sub-Pannonic grassland of the alliance

Festucion vallesiacae in Bistrinci represents one of the

last residuals of steppic grasslands in Croatia.

According to the Ordinance on an Ecological Network,

it is incorporated in a list of the protected areas

important for the species and habitat types in Croatia

under the code HR2000730. A floristic survey was

carried out every two weeks in 2017 and 2018, in a

period from April to November. A total of 136

vascular plant taxa from 45 families were recorded.

The vascular plant taxa were determined, and a

nomenclature was adjusted according to the Croatian

Flora Checklist. The highest number of taxa belonged

to the families of Fabaceae (19 taxa), Rosaceae (13

taxa), Asteraceae (12 taxa) and Poaceae (11 taxa). A

lifeform spectrum analysis proved that the

hemicryptophytes were dominant (71 taxa), followed by the therophytes (27 taxa) and geophytes (17

taxa). Among the identified taxa, 26 are protected, while one species, Dichanthium ischaemum (L.)

Roberty, is strictly protected. Some invasive plant species, such as the Reynoutria japonica Houtt.,

Ailanthus altissima (Mill.) Swingle, and Phytollacca americana L., also occur in the grassland

community. An analysis of the ecological conditions proved that the greatest number of plant taxa are

shade-tolerant, preferring a relatively warm soil, moderately rich in nitrogen. The investigated steppe-

like grassland in Bistrinci represents a unique habitat rich in valuable plants of the Croatian flora;

therefore, conserving this natural resource is of a great importance.

Keywords: East Croatia, steppe-like grassland, vascular flora

12

(Medicine)

ORAL PRESENTATION

The Consumption of the Omega-3-Enriched Eggs Reduces an Oxidative Stress in

Granulocytes of the Healthy Male Participants

Konzumacija jaja obogaćenih omega-3 masnim kiselinama smanjuje oksidativni stres u

granulocitima zdravih muških ispitanika

N. Kolobarić1,2, M. Mihalj1,2, A. Matić1,2, A. Stupin1,2, L. Barić1, Z. Kralik2,3, G. Kralik2,3,

I. Drenjančević1,2*

1Department of Physiology and Immunology, Faculty of Medicine Osijek, Josip Juraj Strossmayer University of Osijek,

Josipa Huttlera 4, HR-31 000 Osijek, Croatia 2Scientific Center of Excellence for a Personalized Healthcare, Josip Juraj Strossmayer University of Osijek, Josipa

Huttlera 4, HR-31 000 Osijek, Croatia 3Department of Special Zootechnology, Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of

Osijek, Vladimira Preloga 1, HR-31 000 Osijek, Croatia


Abstract: The study aimed to investigate the effects

of an n-3 PUFA enriched egg consumption on the

levels of an oxidative stress in human leukocyte

subsets. Thirty‐three healthy participants (aged 20

to 26) were divided into two groups: the first group

consumed the n‐3 PUFA enriched chicken eggs

(OE; n=18, 2 eggs/day, n ‐ 3 PUFA=403.10

mg/day), while the second group consumed the

regular chicken eggs (RE; n=15; 2 eggs/day, n‐3

PUFA=74.63 mg/day) for three weeks. A peripheral

leukocyte oxidative stress was measured by flow cytometry (BD FACS Canto II flow cytometer) with

DCF‐DA (2’,7’-dichlorofluorescin diacetate) assay at the beginning and at the end of the study. The

levels of an intracellular ROS were assessed prior to and 30 minutes subsequent to a phorbol‐12‐

myristate‐13‐acetate (PMA) (1:1000; 50 µL)‐mediated induction of the ROS production. The

statistical analyses were performed using the Sigma Plot and the GraphPad Prism. A t‐test was used

for the differences among the groups, and an intra‐group difference were tested by a paired t‐test,

where p<0.05 was considered significant. A study protocol and procedures conformed to the standards

of the Helsinki Declaration and were approved by the Ethical Committee of the Faculty of Medicine,

University of Osijek. The OE group exhibited significantly reduced levels of ROS upon a PMA

stimulation in the male granulocytes (p=0.0442), in contrast to a significantly increased ROS production

upon a PMA stimulation in the male and female monocytes from the OE respondents (p=0.0006 and

p=0.0227, respectively). The OE men had a significantly lower level of oxidative stress in granulocytes

prior to and upon a PMA stimulation (p=0.0366 and p=0.0049, respectively) if compared to the RE men.

The OE men had the significantly lower levels of ROS in monocytes and lymphocytes if compared to

the OE women (p<0.0001 and p=0.0153, respectively). An omega‐3 consumption reduced the levels

of oxidative stress upon a PMA stimulation in the granulocytes of men, while it exerted an opposite

effect on the monocytes of both sexes. The results suggest that the omega‐3 supplementation reduces

an oxidative stress in granulocytes, probably by increasing a cellular antioxidative capacity.

Keywords: oxidative stress, antioxidative activity, omega‐3 eggs

Acknowledgment

The work is supported by a grant to the Scientific Center of Excellence for a Personalized Healthcare,

Josip Juraj Strossmayer University of Osijek, Croatia.

13

(Medicine)

ORAL PRESENTATION

The Use of Injectable Biphasic Calcium Phosphate in a Guided Bone

Regeneration: A study Plan and Preliminary Results

Uporaba injekcijskog bifaznog kalcijeva fosfata u vođenoj regeneraciji

kosti: plan studije i preliminarni rezultati

M. Pejakić 1, 2*, Ž. Perić Kačarević 2, M. Matijević 2

1 Faculty of Medicine, J.J. Strossmayer University of Osijek, Huttlera 4, HR-31 000 Osijek, Croatia 2 Faculty of Dental Medicine and Health, Josip Juraj Strossmayer University of Osijek, Crkvena 21, HR-31 000 Osijek,

Croatia

*corresponding author: [email protected] / [email protected]

Abstract: A guided bone regeneration (GBR) is a

surgical method used to promote a bone regeneration

in the situations where there is no sufficient height

and width of the residual alveolar ridge to place a

dental implant. An autogenous bone, allografts,

xenografts, and alloplastic materials, in combination

with the resorbable or nonresorbable membranes, are

used in the GBR. The aim of the present study is to

assess the histological and histomorphometrical

changes in the alveolar bone six months subsequent

to the augmentation procedure with an injectable

biphasic calcium phosphate (I‐BCP). The study is

designed as a prospective single‐ arm one and

consists of two stages. In the first stage, twenty

healthy patients with an indication for GBR

subsequent to a tooth extraction and prior to a dental implant placement are enrolled. The indications

for a tooth extraction include a chronic periodontal disease, failure of the endodontic treatment, and a

vertical fracture of the root. A patients’ selection is performed in a dental practice specialized in oral

surgery, and all surgeries are performed by an experienced oral surgeon. Following the tooth extraction,

the intrabony defects are filled with the I‐BCP and covered with a native collagen membrane, to ensure

the isolation of a bone defect next to a soft tissue. Six months subsequent to the healing, simultaneous

to a dental implant placement, bone biopsies for histological analyses are harvested and visualized under

light microscopy. This report presents the preliminary histological findings of I ‐ BCP’s

osteoconductive properties. A histological examination of the specimen revealed a new bone formation

at a peripheral border of the bone defect. The I‐BCP granules of are incorporated into the bone tissue

and there are no histological signs of inflammation. Therefore, we can conclude that a preliminary result

of this study demonstrates an osteoconductive potential of I‐BCP. However, further work is necessary

to complete the histological, radiological, and clinical evidence of I‐BCP’s osteoconductive properties.

Keywords: histomorphometry, bone biopsy, bone substitutes, dental implantology


14

(Medicine)

ORAL PRESENTATION

The Prevalence and Risk Factors for Peer Violence among Adolescents

Učestalost i rizični čimbenici za pojavu međuvršnjačkog nasilja u populaciji

adolescenata

T. Berlančić1, 2, 3*, I. Miškulin1

1 Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Huttlera 4, HR-31000 Osijek, Croatia 2 Faculty of Economics in Osijek, Josip Juraj Strossmayer University of Osijek, Trg Ljudevita Gaja, HR-31000 Osijek,

Croatia 3 University Hospital Center Osijek, Josipa Huttlera 4, HR-31000 Osijek, Croatia


Abstract: Violence among the adolescents is defined

as violence in which persons aged 10 to 21 participate

as victims, aggressors, or witnesses, and there is an

occurrence of an intentional use of physical force or

injury. Violence among the young people is a public-

health and social problem. Violence participants are

usually divided into four groups: “pure” offenders,

i.e., those who commit violence against others,

“pure” victims, victims-abusers, and observers. Peer

violence can also be divided into four categories:

physical, verbal, cyber and relational. The aim of this

study was to determine a frequency and distribution

of violence among the young people at a university

education level according to their age, gender,

academic success and socioeconomic characteristics.

An online, cross-sectional, anonymous pilot study

was conducted during February 2018 by the

application of a specially designed questionnaire,

which contained a multidimensional peer

victimization scale and questions regarding the study participants’ demographic data. The study

involved 329 university students from the University of Osijek aged 20.1 ± 0.8 years (range 18.0 – 21.0),

79.6% being females and 20.4% males. Furthermore, among all study participants, there were 12.8% of

repeaters and 87.2% nonrepeaters. Finally, there were 62.0% of students who were studying in their

hometown and 38.0% of those who were not. The study revealed that the students who were not studying

in their hometown were more exposed to an overall peer victimization, measured by a total bullying

scale (p = 0.009), and were more exposed to social violence, measured by a social violence scale (p =

0.001). The study also demonstrated that the males were more involved in physical violence (p < 0.001),

measured by a physical violence scale. Peer violence is not a well-recognized problem within the

university student population. The results of this study point to the importance of analyzing and

resolving a peer victimization problem at this level of education.

Keywords: peer violence, university students, risk factors, violence, Croatia


15

(Physics)

ORAL PRESENTATION

The Prospects for an Experimental Realization of Two-Dimensional

Aluminum Allotropes


I. Lukačević, M.Varga-Pajtler1, M. Mužević1, S.K. Gupta 2

1 Josip Juraj Strossmayer University of Osijek, Department of Physics, Trg Ljudevita Gaja 6, HR-31000 Osijek, Croatia 2 Department of Physics and Electronics, St. Xavier’s College, Ahmedabad 380009, India

Abstract: Since the discovery of graphene, a number of

atomically thin monoelemental materials have been

predicted and also synthesized, while others wait for an

experimental realization. Motivated by a possible

application potential, owning to the properties of its bulk

counterpart, in this study we predict the possible two‐dimensional allotropes of a monolayer aluminum, called

aluminene, and the conditions in which the aluminene

could be synthesized in a laboratory. The allotropic

modifications considered were planar, buckled,

puckered and triangular. Using the ab initio calculations,

planar and triangular structures demonstrate that, under

certain imposed strain on the monolayer, they possess

the appropriate cohesive energies and lattice dynamics.

The surface of Cu (111) and graphene are found to be

the suitable substrates that stabilize the phonon dynamics of the monolayers. The studied allotropes are

proven to be stable with respect to the thermal effects at room temperature and preserve good thermal

properties (specific heat) of bulk aluminum. The STM images are simulated to assist the experimental

realization and characterization of aluminene.

Keywords: two-dimensional materials, aluminum, structural stability, strain engineering, density

functional theory

16

(Chemistry)

ORAL PRESENTATION

The Optimization of Hydrothermal Method for the Synthesis of Triple

Perovskites with a Sr3Mn2(W/Te)O9 Structure Type

Optimizacija hidrotermalne metode za sintezu trostrukih perovskita

tipa Sr3Mn2(W/Te)O9

D. Goman1,*, J. Bijelić1, A. Stanković1, M. Medvidović-Kosanović1, P. Cop2, B. Smarsly2, I. Đerđ1

1Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Ulica cara Hadrijana 8/A, HR-31000 Osijek,

Croatia 2Justus Liebig University of Giessen, Institute for Physical Chemistry, Heinrich‐ Buff‐ Ring 17, D‐ 35392 Giessen, Germany


Abstract: This study presents an optimization of

hydrothermal synthesis of triple perovskites

Sr3Mn2WO9 and Sr3Mn2TeO9, from an aqueous

solution of metal salt precursors. Usually, a synthesis

of perovskite materials demands a controlled and

long ‐ term annealing. In this research, milder

conditions (a relatively low temperature, 150 ºC and

200 ºC) and shorter reaction time (24 h) were created,

which are environmentally friendly and low-cost. A

continuous development contributes to the new and

more advanced nanosized materials. It is important

for a design of perovskite materials to stabilize the

metal cations in certain oxidation states. For that

purpose, electrochemical measurements were carried out to determine an oxidation state of manganese

cation in the given reaction conditions. The electrochemical results indicate that manganese is in the +3

oxidation state while being transformed into a desired compound. This oxidation state is stabilized by

the usage of a strong base (sodium hydroxide). An interesting fact is that Mn3+ (coordination number 6,

a high spin state) has an ionic radius of 0.645 Å, which corresponds to the ionic radius of Fe3+ (also

coordination number 6, a high spin state). Hence, a structural similarity to Sr3Fe2WO9 and Sr3Fe2TeO9,

is expected. In conclusion, the composition, morphology, and stability of prepared compounds were

investigated by means of the powder X‐ ray diffraction (PXRD), Fourier‐ transform infrared

spectroscopy (FT-IR), thermogravimetric analysis coupled with the differential scanning calorimetry

(TGA/DSC) and the X‐ray photoelectron spectroscopy (XPS).

This research is a part of the Croatian Science Foundation project entitled Investigation of Complex

Multiferroic Materials Prepared by the Solution Chemistry Methods under the supervision of Prof. Igor

Đerđ, Ph.D.

Keywords: perovskite, hydrothermal synthesis, nanomaterials

Acknowledgments

The authors express their gratitude to the Croatian Science Foundation (IP‒2016‒06‒3115) for a full

financial support and to the DAAD for a partial support via German‐Croatian exchange partnership

(ID 57335028).

17

(Chemistry)

ORAL PRESENTATION

A Coprecipitation Synthesis and Modification of Magnetic Spinel Ferrite

CoFe2O4 Nanoparticles with Gold

Koprecipitacijska sinteza i modifikacija zlatom magnetskih spinelnih

feritnih nanočestica CoFe2O4

M. Bjelić1,*, T. Šafarik1, A. Széchenyi1

1 Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Ulica cara Hadrijana 8/A, HR-31000 Osijek,

Croatia


Abstract: The magnetic cobalt ferrite nanoparticles

(CoFe2O4) with an average diameter from 10 to 80

nm are important because of their wide application,

especially in biomedicine, where they are used as the

MRI contrast enhancers, for a drug delivery and

magnetic hyperthermia. The aim of this study is to

synthesize the cobalt ferrite nanoparticles with a

desired particle size and to monitor the effects of

certain reaction parameters, which are mentioned

bellow. The cobalt ferrite nanoparticles were

synthesized by the coprecipitation method, whose

main disadvantage is the control of a nanocrystal size.

To solve that problem, it is necessary to achieve an

accurate control of different reaction conditions, such

as the mixing order of synthesis components, reaction

temperature, and a reaction pH value. A nanoparticle

modification with nitric acid and gold does not only provide stability to the nanoparticles in solution but

it also assists to the binding of various biological ligands to a nanoparticle surface. The covering of the

synthesized nanoparticles with a layer of gold is achieved by a reduction of HAuCl4 in the presence of

a strong reduction agent. The synthesized nanoparticles were characterized by a dynamic light scattering

(DLS), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR),

electrophoretic light scattering (ELS), and thermogravimetric analysis (TGA). The obtained results

illustrate that the smaller particles are formed at a lower temperature. The FT‐IR spectra confirmed

the binding of the NO2 functional group onto the nanoparticle surface, which has a great influence on

the suspension stability. Also, by lowering the suspension’s pH value, the amount of amorphous

substance is reduced, and the TEM images show that the cobalt ferrite nanocrystals without amorphous

contaminants are present at pH 3. A future goal of this work is to apply the gold‐coated cobalt ferrite

nanoparticles as nanosensors.

Keywords: cobalt ferrite, modification, gold, reaction parameters

Acknowledgments

The authors express their gratitude to Dr. Hajnalka Ábrahám and Prof. Dr. László Seress (University of

Pécs, Faculty of Medicine, Department of Medical Biology and Central Electron Microscope

Laboratory) for the assistance and support during the sample analysis with the TEM. Some TEM studies

were performed using the JEOL‐1400 TEM electron microscope funded by the GINOP‒2.3.3‒15‒

2016‒0002 grant (a new generation electron microscope, 3D ultrastructure).


18

(Chemistry)

ORAL PRESENTATION

Electrochemical Detection of As3+ Ions


Ž. Kajinić 1,*, J. Vešligaj Turkalj2, Ž. Romić 2, A. Stanković 1, M. Medvidović-Kosanović 1


Croatia 2 Vodovod, Poljski put 1, HR-31000 Osijek


Abstract Arsenic is a naturally occurring, widely

found chemical element. The inorganic forms of

arsenic are generally considered to exert a highly

toxic effect on human health and the environment.

The greatest concerns for public health pertaining to

the arsenic compounds originate from a contaminated

groundwater or soil, which contain the inorganic

forms of arsenic, mainly the arsenite (III) and

arsenate (V). According to the recent

epidemiological studies, the association of an arsenic

exposure and cancer, hypertension, or kidney disease

has been identified. The aim of this study is an

electrochemical detection of As3+ ions using a modified and an unmodified glassy carbon electrode in

the model systems, as well as in the groundwater samples. An electrochemical analysis was carried out

by means of a cyclic voltammetry and differential pulse voltammetry. Based on the obtained results, the

most adequate method was determined. The measurements were performed in a Faraday cage using a

potentiostat/galvanostat (PalmSens) controlled by the PSTrace 1.2. software package. A three‐electrode system consisted of a glassy carbon working electrode, an Ag/AgCl reference electrode, and

of a platinum wire counter electrode. A regeneration of the working electrode surface was carried out

by polishing it with an α-Al2O3 suspension, and it was subsequently cleaned in an ultrasonic bath when

necessary. Furthermore, the electrochemical system was purged with a purified argon in order to

deaerate the system, more precisely to avoid an oxygen contamination during measurements.

It has been found that an unmodified glassy carbon electrode did not provide any electrochemical

response to arsenic in the potential range from -1 V to +1 V either by a cyclic or by a differential pulse

voltammetry. Therefore, the glassy carbon electrode surface has been modified, according to a

procedure found in literature, in order to detect the As3+ ions in standard solutions and groundwater

samples. Differential pulse voltammetry was found to be a more sensitive method for As3+ detection if

compared to the cyclic one, which was especially evident in the groundwater sample analysis, where

the pronounced oxidation peaks in a measured potential range were obtained.

Keywords: arsenic, groundwater, glassy carbon electrode, cyclic voltammetry, differential pulse

voltammetry

0.00

2.00

4.00

6.00

8.00

-0.100 0.100 0.300 0.500

I /

µA

E / V

0

5

10

15

20

0 5 10 15 20 25

I p,a

/ m

A

106 c / M

19

(Chemistry)

ORAL PRESENTATION

An Aqueous Sol-Gel Synthesis of Double and Triple Perovskites with an

A2Ni(Te/W)O6 and A3Co2TeO9 Structure Type

Vodena sinteza sol-gel dvostrukih i trostrukih perovskita tipa A2Ni(Te/W)O6

i A3Co2TeO9

M. Sekulić1, *, J. Bijelić1, A. Stanković1, M. Medvidović-Kosanović1, P. Cop2, B. Smarsly2,

I. Đerđ1


Croatia 2 Institute for Physical Chemistry, Justus Liebig University of Giessen, Heinrich-Buff-Ring 17, D-35392 Giessen,

Germany



Abstract: Perovskites are the compounds with a

general formula ABO3 (A = larger cation, B = smaller

cation, O = oxygen). The ideal perovskites crystallize

in a cubic crystal system that can be predicted by the

Goldschmidt tolerance factor formula. Their usage in

modern life is widespread, mostly in the digital world,

where the emphasis is on the size reduction of the

electronic devices and on an expanding density of

data storage. The sol‐gel chemistry describes a

transformation of a liquid precursor into a sol and

finally into a gel. These methods are very sensitive

because different structures may be obtained using

the same precursors, with small modifications in the

reaction conditions. This study presents a citrate sol-gel route of complex metal oxides with a perovskite

crystal structure type previously reported in similar compounds. The dried samples were calcined in two

steps, at 600 ˚C and 950 or 1000 ˚C. The synthesized samples were investigated by the powder X‐Ray

diffraction to determine a phase composition, FT‐IR spectroscopy to determine the presence of organic

impurities, and by a thermogravimetric analysis coupled with a differential scanning calorimetry

(TGA/DSC) to analyze a thermal stability of the compound and possible phase transitions. The

electrochemical measurements were conducted in order to determine an oxidation state of transition

metals in the reaction mixture and to determine the reaction mechanism. The in‐situ X-ray diffraction

measurements were conducted up to 900 ˚C to identify the structural changes during calcination. The

results revealed the successfully synthesized tellurium ‐ based metal oxides with a double

(A2Ni(Te/W)O6, A = Sr, Ba) and triple (A3Co2TeO9, A = Sr, Ba) perovskite structure type.

This research is a part of the Croatian Science Foundation project entitled Investigation of Complex

Multiferroic Materials Prepared by the Solution Chemistry Methods under the supervision of Prof. Igor

Đerđ, Ph.D.

Keywords: perovskites, sol‐gel methods, citrate route

Acknowledgments

The authors express their gratitude to the Croatian Science Foundation (IP‒2016‒06‒3115) for a full

financial support and to the DAAD for a partial support via German‐Croatian exchange partnership

(ID 57335028).

20

(Chemistry)

ORAL PRESENTATION

A Synthesis and Characterization of Cu(II), Fe(III), and Cr(III)

Polynuclear Carboxylate Complexes

Sinteza i karakterizacija polinuklearnih karboksilatnih kompleksa

bakra(II), željeza(III) i kroma(III)

S. Zorić, T. Balić*

Josip Juraj Strossmayer University of Osijek, Department of Chemistry, Ulica cara Hadrijana 8/A, HR-31000 Osijek,

Croatia


Abstract: The studies of structural features of

polynuclear metal carboxylate complexes (known as

the “basic carboxylates”) are recently gaining a lot of

attention because of their interesting magnetic and

electronic properties. These compounds display

different magnetic properties (ferro‐, ferri‐, and

antiferromagnetic), depending upon a molecular and

crystal structure. They can also be used as the

precursors for the synthesis of higher nuclearity

clusters. In this work, the polynuclear complexes

with Cu(Ⅱ), Fe(Ⅲ), and Cr(Ⅲ) were synthesized by

the reactions of acetic and benzoic acid with the

appropriate metal salts in the presence of template molecules (hexamine and guanidine) at room

temperature. The prepared complex compounds were characterized by a single crystal X‐ ray

diffraction (sc‐XRD), FT‐IR spectroscopy, and elemental analysis. Under these conditions, the

crystals of two novel polynuclear (Fe(Ⅲ) and Cu(Ⅱ)) complexes were obtained and characterized by

sc‐XRD (FeHEXOK and CuGUABA). An X‐ray structure analysis of FeHEXOK has demonstrated

that the compound crystallizes in an orthorhombic crystal system, the P nma space group (Z=4). The

complex compound is comprised of one Fe-basic acetate unit (trinuclear), a noncoordinated nitrate anion,

a hexamine molecule, and of the crystallization molecules’ water. A positive charge of the central Fe-

basic acetate unit is neutralized by the presence of a nitrate anion in a crystal structure, and a formal

oxidation state of all three iron atoms is +3 (d5 configuration). In the crystal structure, the molecules are

primarily linked via strong O‒H∙∙∙N and O‒H∙∙∙O hydrogen bond interactions. An X-ray structure

analysis of CuGUABA has proven that the compound crystallizes in a triclinic crystal system, the P -1

space group (Z=2). Interestingly, in the crystal structure of CuGUABA there are several different

dinuclear Cu‐benzoate moieties. A molecular structure of the discrete moieties is a typical paddle‐

wheel one, often observed in the Cu‐carboxylate complexes. The results of FT‐IR spectroscopy and

elemental analysis are in agreement with proposed structures. Several other Cr(Ⅲ), Cu(Ⅱ) and Fe(Ⅲ)

complexes were synthesized and characterized by FT‐IR and elemental analysis.

Keywords: polynuclear complexes, carboxylate, copper, iron(Ⅲ), chromium(Ⅲ)

21

(Chemistry)

ORAL PRESENTATION

A Synthesis of Coumarin Derivatives via Knoevenagel Condensation in

Green Solvents

Sinteza derivata kumarina Knoevenagelovom kondenzacijom u zelenim

otapalima

M. Lončarić*, M. Molnar

Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology, Department of Applied Chemistry and Ecology,

Franje Kuhača 20, HR-31 000 Osijek, Croatia


Abstract: Nowadays, the biologically active

compounds attract considerable attention due to

their various biological and pharmaceutical

properties. Many of these compounds can be

extracted from plants, but due to their increased

use for medical purposes, they are increasingly

synthesized in laboratories. Coumarins are an

important class of biologically active compounds

and play an important role in nature, acting as the

plant growth hormones and growth regulators,

participating in a respiration control,

photosynthesis, and defense against infections.

Coumarins are also attributed to a range of medical

applications, such as the anticancerogenic, antioxidant, and anti‐HIV one, acting as an enzyme

inhibitor, vasorelaxant, anticoagulant, an antibacterial agent, and much more. Among many reactions

for coumarin synthesis (the Pechmann condensation reaction, Witting, Claisen, Reformatsky, and

Kontanecki‐Robinson reaction), the most used reaction is the Knoevenagel condensation. An

important factor in chemical synthesis is a usage of the environment‐friendly solvents. One of the

green approaches in synthesis is a usage of the deep eutectic solvents (DESs) as the reaction media. The

DESs possess many desired properties, such as a low toxicity, high availability, low inflammability,

high recyclability, low volatility and low price.

The aim of this work was to synthesize the coumarin derivatives via Knoevenagel condensation using

the green solvents (DESs) under conditions established to be the best ones by a previously performed

screening. A model reaction of salicylaldehyde and dimethyl malonate was performed in 20 different

DESs at 80 °C. Choline chloride was used as a hydrogen bond acceptor, and 20 other components like

urea, carboxylic acids, sugars or amides were the hydrogen bond donors. ChCl:Urea (ChCl:U, 1:2) is

proven to be the best solvent, which afforded a yield of 50.5%, followed by the ChCl:N-methylurea

(1:3) with a yield of 45.1%. Based on the obtained results, a series of coumarin derivatives was

synthesized from the substituted salicylaldehydes and dimethyl malonate, diethyl malonate, or ethyl

benzoylacetate.

Keywords: deep eutectic solvents, synthesis, coumarins

Acknowledgement

This work is partially supported by the Croatian Science Foundation within the project Green

Technologies in Synthesis of Heterocyclic Compounds (UIP-2017-05-6593).

22

(Agriculture)

ORAL PRESENTATION

The Advantages of Lime Application on Acid Soils

Prednosti kalcizacije kiselih tala

D. Iljkić*, M. Rastija, V. Kovačević

Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-



Abstract: An open‐field agricultural production is

very often exposed to many abiotic and biotic

stresses. Soil acidity, unfavorable weather

conditions, and an unbalanced nutrient supply are

the main limiting factors of a field crops yield and

quality worldwide, as well as in Croatia. Many

authors estimated that 25 to 40% of global areas had

a higher or lower level of soil acidity, and a similar

situation is in Croatia. The main problem of this soil

is toxicity of aluminum, manganese, and iron on one

hand and a lack or an inaccessibility of elements

such as phosphorus, calcium, and magnesium on the other hand. The aim of this study was to

demonstrate a residual effect of liming to a grain yield and the quality of field crops. A field trial was

set up in 2011 on an acid soil (pHKCl 4.01) in a randomized complete block design (RBCD) in four

replicates and four treatments (0 t ha-1, 3.5 t ha-1, 7.0 t ha-1 and 14.0 t ha-1). Generally, liming exerted a

positive effect on some chemical properties of the soil. Furthermore, in all three vegetation periods of

winter wheat and two periods of maize a residual effect of liming material was significant for the yield,

some tested parameters, and quality. The highest treatment increased the wheat yield by 22, 25, and

32% and the maize yield by 11%, depending of a year. At the same time, a protein content was higher

by 8% in the highest treatment. Based on many field trials of ours, liming exerted a very positive effect

on a grain yield and a quality and soil status. However, these effects depend on many agroecological

conditions, such as a degree of soil acidity, weather conditions, the time and the amount of application,

etc.

Keywords: liming, yield, wheat, maize, quality

23

Abstract: The Carniolan honey bee (Apis mellifera

carnica, Pollmann 1879) is an indigenous and unique

honey bee subspecies in Croatia, characterized by its

successful overwintering, explosive spring

development, diligence and a good gathering activity,

low aggressiveness, and a pronounced swarming

behavior. In our study, we compared the differences

in the characteristics of an overwintering success,

colonies development, behavior, and disease

resistance traits in five different lines of Carniolan

honey bees. The Groups 1 to 3 were of a local origin,

selected in five generations. Group 4 was of a local

origin and was never subject to an artificial selection.

The queens from Group 5 were from Germany and

under selection for > 10 generations. The results of the study did not demonstrate significant differences

between the number of bees and brood cells, but a delayed spring development of Group 5 was recorded,

compared to other groups. The lowest overwintering index subsequent to both winters was recorded in

Group 5, as well as a consumption of food during winter. The worst score for a defensive behavior was

achieved by Groups 4 and 3 and were significantly worse than Group 5. The lowest score for the

calmness on comb was achieved in Group 4, significantly worse than in all other groups. A swarming

behavior was mostly manifested in Group 4, which was significantly worsely rated than the Groups 1,

3, and 5. In honey production and pollen collection there were no significant differences between the

groups, as well as in a hygienic behavior, grooming, and a suppressed mite reproduction (SMR). In all

the groups there was a uniform development of the Varroa population, and in Groups 4 and 5 there was

a slightly lower population of mites when compared to the first three groups. Finally, Group 4 had a

significantly worse selection index than Group 5, while no significant differences were found among

other groups. The research results clearly indicate an importance of breeding the locally adapted bees.

Keywords: Apis mellifera carnica, breeding, selection, traits, Varroa destructor

(Agriculture)

ORAL PRESENTATION

The Influence of Selection on the Traits of the Carniolan Honey Bee (Apis

mellifera carnica) in Croatia

Utjecaj selekcije na osobine sive medonosne pčele (Apis mellifera carnica)

na području Hrvatske

M. Kovačić 1,*, Z. Puškadija1, R. Büchler2

1 Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-

31000 Osijek, Croatia 2 State Department for Agriculture of the Land of Hessen, Apicultural Institute Kirchhain, Erlenstraße 9, 35274

Kirchhain, Njemačka


mailto:*corresponding%20author:%[email protected]

24

(Agriculture)

ORAL PRESENTATION

Plant Substances and Inert Dusts – Natural Insecticide Formulations in

the Storage Insects Control

Biljne supstance i inertna prašiva – prirodne formulacije insekticida

u kontroli skladišnih kukaca

P. Lucić*, V. Rozman

Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-31

000 Osijek, Croatia


Abstract: The aim of the research was to determine the

efficacy of botanical insecticides in the form of a powder

and extract, inert dusts, and a combination of botanical

insecticides and inert dusts in the control of three stored-

product insects: Rhyzopertha dominica (Fab.), Sitophilus

oryzae (L.), and Tribolium castaneum (Herbst). The

research was conducted in laboratory conditions by a

series of experiments in glass containers filled with wheat

and by an application on various surfaces. Nine plant

species were tested as powders and extracts, as well as the

six inert dusts of a Croatian origin (named after their

location, i.e., MA ‐ 4, where “MA” stands for

Markuševec and the number four stands for a sample

number), together with a diatomaceous earth Celatom

Mn-51®. The tested plant powders on wheat indicate that

a satisfactory mortality (91.0%) was produced only with

regard to the R. Dominica, treated by lavandin in a dosage amounting to 8,000 mg kg-1. The application

of plant extracts on glass surface produced the highest mortality of all insect species. The results of the

tested inert dusts demonstrated that the highest mortality (90.0%) of R. dominica was produced with

Celatom Mn-51®, as well as the highest inhibition of progeny (99.19%), while a maximum mortality of

S. oryzae was produced with the inert dusts D-01, MA‐4, and Celatom Mn-51®, which also provoked

a complete progeny inhibition. A maximum mortality of T. castaneum was produced with Celatom Mn-

51®, and a complete progeny inhibition was achieved with the inert dusts D-01, MA‐4, and Celatom

Mn-51®. A significant reduction of the test weight was observed at the highest LD90 doses in the

treatments with Celatom Mn-51® and inert dusts PD-1 and MR-10. The results of a tested mixture of

plant powder and inert dust indicate that the highest mortality (68.0%) of R. dominica was produced

with a combination of Celatom Mn-51® and lavandin at a 1:15 ratio, and the highest progeny inhibition

(93.83%) was also achieved in the same treatment.

Keywords: plant powder, inert dust, Rhyzopertha dominica, Sitophilus oryzae, Tribolium castaneum

Acknowledgment

This doctoral dissertation was funded by the Croatian Science Foundation within the project IP‒11‒

2013‒5570.


25

(Agriculture)

ORAL PRESENTATION

The Influence of Physical and Chemical Properties of Different Soil

Types on the Optimal Soil Moisture for Tillage

Utjecaj fizikalnih i kemijskih svojstava različitih tipova tla na optimalno

stanje vlažnosti za obradu

V. Zebec1,*, Z. Semialjac1, M. Marković1, V. Tadić1, D. Radić2, D. Rastija1

1Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-



Abstract: Soil plasticity is an area of soil

consistency, i.e., it represents a change in soil

condition due to a different soil moisture

influenced by the activity of external forces.

Consistency determines a soil resistance in

tillage; therefore, the aim of the research was to

determine the optimum soil moisture condition

for tillage and the influence of chemical and

physical properties of the arable land horizons

with regard to the soil plasticity of three different

types of soil (fluvisol, luvisol, and humic gleysol).

Statistically significant differences were found

between all examined soil types, such as the

content of clay particles, density of packaging and

actual and substitution acidity, cation exchange

capacity and the content of calcium. There were

also statistically significant differences between the examined types of soil pertaining to a plasticity

limit, liquid limit, and plasticity index. The average established value of plasticity limit, as an important

element for determining the optimal moment of soil tillage, amounted to 18.9% of the mass on fluvisol,

24.0% of the mass on luvisol, and 28.6% of the mass on humic gleysol. A very significant positive

direction correlation with plasticity limits was manifested by an organic matter, clay, fine silt,

magnesium, sodium and calcium, while a very significant negative direction correlation was manifested

by a hydrolytic acidity, coarse sand, fine sand and coarse silt. The created regression models may

estimate the optimal soil moisture condition for soil cultivation based on the basic soil properties. A

model precision is significantly increased by the introduction of a greater number of agrochemical and

agrophysical soil properties, and additional precision of the model may be increased by the soil type

data.

Keywords: soil plasticity, physical and chemical soil properties, soil type

Water contetnt

Vo

lum

e

solidsemi -solid

plastic liquid

SL PL LL

Consistency limit

0 20 40 60 80

All samples

Fluvisol

Luvisol

Humic gleysol

Plastic properties on investigated soils (% mass)

Plastic limit Liquid limit

26

(Agriculture)

ORAL PRESENTATION

The Impact of High-Voltage Electrical Discharge on Winter Wheat under

Drought

Utjecaj visokonaponskog električnog pražnjenja na ozimu pšenicu

u uvjetima suše

M. Ižaković1,*, K. Jukić2, T. Marček1

1 Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31000 Osijek,

Croatia

2 BC — Institute for Breeding and Seed Production of Field Crops Zagreb


Abstract: A modern wheat‐breeding industry is

mostly focused on the production of genotypes,

which substantiate a quality of grain and flour, high

grain yield, and a resistance to stalk lodging and

environmental stresses, including the pathogens

and different abiotic stress factors. Drought is the

most frequent abiotic stress factor that causes huge

economic losses in the yields worldwide. The

plants avoid drought by involving the complex

mechanisms that prevent a water loss, including a

stronger root development. An early

developmental stage like germination is especially

sensitive to water shortage. An improvement of

seed germination may be achieved by various

nondestructive physical methods that change the

chemical properties of a seed coat while creating a special wetting ability. The chemical methods are

frequently used for this purpose, but due to their negative impact on human health and the environment

their usage should be minimized as much as possible. A high‐voltage electrical discharge (HVED)

treatment is a new, physical technology in agricultural practice, which can promote a seed germination

and a growth of seedlings. In this study, the effect of an HVED pretreatment on the wheat’s winter

genotypes exposed to drought was tested. Prior to the drought treatments, the seeds of two wheat

genotypes were treated with HVED. The effect was tracked by measuring a germination rate,

germination percentage, and morphometric parameters, such as a shoot and root length during several

days under control, and a restricted water regime. The drought was simulated by the application of

polyethylene glycol 6000 (PEG), a moisture‐deficit, stress‐inducing medium. This research has

provided a potential of a noninvasive HVED method in the improvement of drought tolerance pertaining

to the winter wheat genotypes in an early developmental stage. Moreover, the results of this study may

be beneficial for the breeders in a selection of genotypes with the best traits.

Keywords: HVED pretreatment, wheat, germination, drought

27

(Agriculture)

ORAL PRESENTATION

The Increase of Selenium Content in Eggs by the Usage of Biofortified

Maize in Hen Mixtures

Povećanje sadržaja selena u jajima korištenjem biofortificiranog kukuruza

u hrani kokoši

B. Ljuboja*, Z. Kralik*

Faculty of Agrobiotechnical Sciences Osijek, Josip Juraj Strossmayer University of Osijek, Vladimira Preloga 1, HR-


*corresponding authors: [email protected]; [email protected]

Abstract: The aim of the study was to determine an

influence of the selenium‐biofortified maize on an egg

quality and an increase of selenium content in eggs at two

levels (a lower and a higher one). A trial period lasted

for four months, and a total of 60 hens of the Hrvatica

breed, red strain, were used. The hens were divided into

three experimental groups (K, P1, and P2). The hens

from the K group represented a control group, and they

consumed a feed mixture of a commercial composition,

while the hen groups P1 and P2 consumed the mixtures

in which a commercial maize was replaced with a

biofortified one. The Bergxxon maize hybrid (RWA, FAO group 400) was foliarly biofortified with

selenium on two levels (P1=10 g Se ha-1 and P2=20 g Se ha-1). The feed mixtures were balanced up to

18% of crude protein and 11.4 MJ ME/kg of feed. A premix used in the mixtures of the experimental

groups did not contain selenium in its composition. The usage of the selenium‐biofortified maize in

the feed of the Hrvatica hen breed increased a selenium content in the edible part of eggs (P<0.001)

statistically significantly. Also, a statistically significantly higher selenium content was found in the egg

yolks (K=0.2156 mg kg-1; P1=0.4256 mg kg-1 i P2=0.5180 mg kg-1) compared to the egg whites

(K=0.0619 mg kg-1; P1=0.1530 mg kg-1 i P2=0.2028 mg kg-1; P<0.001). The used feeding treatments

exerted a positive effect on the fresh and stored eggs’ quality indicators. Based on the analysis results,

it is recommended to use the biofortified maize in hen nutrition. A reason for the recommendation is a

fact that it is already possible to replace selenium in the premix with a lower level of biofortified maize,

which is used in the feed in the amount of 56.5%.

Keywords: hens, corn, biofortification, selenium, egg quality

Acknowledgment

The results used in the paper are a part of the research on the project Enriching the Eggs of the Hrvatica

Hens with the Essential Microelements, funded by the Council for Research in Agriculture in the period

from 2015 to 2017.

28

(Food technology)

ORAL PRESENTATION

The Influence of Processing Parameters on the Retention of Aroma

Compounds during Wine Concentration by a Reverse Osmosis and

Nanofiltration

Utjecaj procesnih parametara na zadržavanje spojeva arome tijekom

koncentriranja vina reverznom osmozom i nanofiltracijom

I. Ivić,*, A. Pichler

Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology Osijek, Franje Kuhača 20, HR-31000 Osijek,

Croatia


Abstract: The reverse osmosis and nanofiltration are

based on a selective membrane application. One

sample fraction passes through the membrane as a

permeate, and the other fraction is retained on the

membrane as a retentate. Both processes can be used

for the cleaning or demineralization of water and for

the concentration of wine, must, milk and other

beverages. In the wine industry, they are mostly used

for must correction, must and wine concentration, or

for the reduction of an alcohol content in wine.

During wine concentration, water and ethanol are

passing through the membrane as a permeate, but,

depending on the processing parameters (pressure,

temperature, and membrane type), some of the aroma compounds can also pass through (usually those

with a lower molecular weight). Therefore, many studies on the retention of aroma compounds during

wine concentration have been conducted.

The aim of this study was to compare the influence of reverse osmosis and nanofiltration membranes

on the retention of volatile compounds during a concentration of white wine, at two temperatures (20 °C

and 30 °C), on a laboratory filter with a plate module. The results demonstrated that, during the wine

concentration by a reverse osmosis, the retention of aroma compounds was higher (especially the

retention of aldehydes and ketones) when compared to that by nanofiltration. Both processes resulted in

a high terpene retention (almost 100%). An increase in temperature did not have a major effect on the

aroma retention during a reverse osmosis, but during nanofiltration the retention of acids in wine was

significantly lower at 30 °C. As for the ethanol in both processes, it passed through a membrane with

water, but at the end of the process the ethanol content in the permeate and in the retentate was almost

equal.

Keywords: reverse osmosis, nanofiltration, wine concentration, retention of aroma compounds

29

(Biotechnology)

ORAL PRESENTATION

The Identification of Headspace Compounds, Volatiles, Fatty Acid

Content and Antifungal Activity of the Extracts of Macroalga Codium

bursa

Identifikacija vršnih para, isparljivih komponenata, sastava masnih kiselina

te antifungalna aktivnost ekstrakata makroalge Codium bursa

A.-M. Cikoš1,*, I. Jerković2, B. Šarkanj3, S. Jokić1

1 Faculty of Food Technology, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia 2 Department of Organic Chemistry, Faculty of Chemistry and Technology, University of Split, HR-21000 Split, Croatia 3 University North, Department of Food Technology, Trg dr. Žarka Dolinara 1, HR-48000 Koprivnica, Croatia


Abstract: Belonging to the family Codiaceae and to

the phylum Chlorophyta, the green macroalga Codium

bursa is considered to be a valuable source of

compounds with various bioactivity. This study aimed

to investigate its phytochemical composition and

antifungal activity compared to the most common

mycotoxigenic fungi, such as the Aspergillus flavus,

Aspergillus ochraceus, Fusarium graminearum,

Fusarium verticillioides, Penicillium expansum.

Codium bursa (Olivi) C. Agardh was collected in May

2018 from the Adriatic Sea and was subsequently

air‐ and freeze‐dried until analysis. The headspace

compounds were extracted by a headspace solid‐

phase microextraction (HS ‐ SPME), while the

volatiles were extracted by hydrodistillation (HD) and a supercritical CO2 extraction (SC‐CO2),

followed by gas chromatography and a mass spectrometry analysis (GC‐MS). A fatty acid content was

determined by gas chromatography with a flame ionization detector (GC‐FID) subsequent to a

derivatization as methyl esters. The results demonstrated that a chemical composition of both headspace

compounds and volatiles was remarkably different, depending on a drying process and a method used

for extraction. Dimethyl sulphide was a major compound found in the headspace of a fresh C. bursa,

while in an air‐dried C. bursa it was present as a minor constituent. In the distillate of a fresh C. bursa,

heptadecane and docosane were found as the major compounds, while (E)‐phytol was a predominant

compound found in the distillate of an air‐dried C. bursa, as well as in a SC‐CO2 extract of a freeze‐dried C. bursa. The presence of loliolide was determined only in a SC-CO2 extract. Hence, the palmitic

acid (25.4%) was found as a dominant fatty acid, followed by the oleic (36.5%), linoleic (11.6%), and

stearic acid (9.0%). A DMSO extract of a freeze‐dried C. bursa produced a good antifungal effect

against the fruit‐associated storage fungi Penicillium expansum. This alga can be of a great interest for

future investigations considering its phytochemical profile.

Keywords: Codium bursa, extraction, volatiles, fatty acids, antifungal activity

30

(Food technology)

ORAL PRESENTATION

The Interaction of Fullerenol C60(OH)24 Nanoparticles with the

Aflatoxigenic Foodborne Fungi

Interakcija nanočestica fulerenola C60(OH)24 s aflatoksikogenom plijesni

prisutnom u hrani

T. Kovač 1,2*, I. Borišev 3, A. Lončarić 1, C. N. Ezekiel 2, 4, M. Sulyok 2,

R. Krska 2, 5, B. Šarkanj 1, 2, 6

1 Josip Juraj Strossmayer University of Osijek, Faculty of Food Technology, Department of Applied Chemistry

and Ecology, Franje Kuhača 20, HR-31000 Osijek, Croatia 2 Center for Analytical Chemistry, Department of Agrobiotechnology (IFA‐Tulln), University of Natural Resources

and Life Sciences Vienna (BOKU), Konrad-Lorenzstraße 20, 3430, Tulln, Austria 3 University of Novi Sad, Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection,

Trg Dositeja Obradovića 3, 21000 Novi Sad, Serbia 4 Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria 5 Institute for Global Food Security, School of Biological Sciences, Queen’s University Belfast, University Road, Belfast,

BT7 1NN, Northern Ireland, United Kingdom 6 University North, Department of Food Technology, Trg dr. Žarka Dolinara 1, HR-48000 Koprivnica, Croatia


Abstract: The fullerenols, C60(OH)24

(FNPs), are the water‐soluble fullerene

C60 daughter products that possess a great

potential for a modulation of biosynthetic

pathways of the secondary metabolites in

the foodborne aflatoxigenic fungi.

In order to clarify the extent, an FNP –

aflatoxigenic fungi interaction, the

impact of different FNP concentrations

(0, 10, 100 and 1,000 ng mL-1) on an

aflatoxin biosynthesis pathway, and a production of emerging toxins (a non‐aflatoxin biosynthetic

pathway) from Aspergillus flavus NRRL 3251 were examined. The fungi were cultivated at 29 °C in the

dark, in an extract‐sucrose (YES) medium during a 168‐hour growth period. A diameter of the FNPs

used in this study amounted to 8 nm, while a determined ζ potential amounted to -33 mV. A mycelial

growth was affected at 1,000 ng mL-1, while a conidial production was slightly affected at 10 ng mL-1.

The mycelia and media samples contained most of the monitored aflatoxin precursors, with an exception

of the norsorolinic acid. Furthermore, the observed FNP effect was concentration‐dependent, and a

sterigmatocystin export from the cells was altered. An antioxidative effect of FNPs subsequent to 120

hours of the growth period vanished and caused a strong concentration‐dependent aflatoxigenic effect

till the end of the growth period (168 hrs). In case of the emerging toxins, ten of them were produced in

a sum of total concentrations reaching 1,745,035 ng 50 mL-1 of the YES medium, while three of them

were found only in the mycelia. The observed ratio of mycelia:media distribution amounted to 99:1. An

amount of 1,000 ng mL-1 of FNPs increased the medial concentrations, while an increase in the applied

concentrations of FNPs provoked a decrease of the observed mycelial‐emerging toxin concentrations.

In addition, cordycepin was confirmed as an A. flavus secondary metabolite for the first time. An

outcome of the presented study points out the relevance of a risk assessment concerning the

nanoparticles and their availability in the environment in the time of global climatic changes.

Keywords: fullerenol C60(OH)24 nanoparticles, Aspergillus flavus, aflatoxin biosynthetic pathway,

emerging toxins, global climatic change


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Josip Juraj Strossmayer University of Osijek · Josip Juraj Strossmayer University of Osijek I Sveučilište Josipa Jurja Strossmayera u Osijeku Department of Biology (University