Summary:

Molecular biologist with 14 years of lab experience including assay development, cloning, protein purification, and genetic screening. I’m currently performing RNA sequencing (Illumina HiSeq) and analysis of gene expression from mutant RNA polymerases.

My postdoc work at the National Cancer Institute was on development of novel assays to capture mistakes made during mRNA synthesis—we’ve published the only known reports of such systems (Irvin et. al. PLOS Genetics 2014 and Malagon et. al. J. Biol Chem 2013). The genetic screens we devised provide the first answers to the 60-year-old biological questions about the accuracy of transcription.

I have managed 18 scientists with diverse backgrounds, and taught university Microbiology classes. I also have experience in scientific program management at the FDA and the CDMRP (US Army Medical Research) managing scientific portfolios of almost 200 grants and contracts worth nearly $250,000,000.

I'm located in Maryland and am willing and able to relocate without assistance for the right scientific position.

Jordan Irvin, PhD(814) 574-1027jordanirvin@gmail.comwww.linkedin.com/pub/jordan-irvin/2/232/92/

Education:2005 PhD Biochemistry, Microbiology, and Molecular Biology, Penn State University1998 BS Biology, with Honors, Indiana University

Technical Skills: 14 years of experience in molecular biology, strain engineering and

genetics. Cloned, purified, and sequenced almost 400 plasmids and made approximately 900 yeast strains

Developed novel assays to study errors in mRNA synthesis Illumina HiSeq RNA sequencing to analyze differences in gene

expression Cell biology assays: immunofluorescence, microscopy,

chemical/genetic screening Microarrays, chromatin immunopurification, nucleic acid

purification, Southern, northern, and western blotting, PCR, cloning, protein purification

laboratory equipment including robotic cDNA microarrayer, high-throughput liquid plate handlers, ultracentrifuges, HPLC

eukaryotic cell culture up to 20 liter scale management and mentoring of 18 younger scientists over 14 years Scientific grant management; ~200 grants and contracts totaling

~$200,000,000 in 19 different disease topic areas including 40 clinical trials

Professional and Research ExperienceNovember 2014 - present. Research Scientist, National Cancer Institute, Frederick, MD. Gene Regulation and Chromosome Biology LaboratoryInvestigating the accuracy of gene expression using biochemical, genetic, and cell biology

Investigating RNA polymerase mutants using Illumina HiSeq gene expression arrays and RNAseq technologies

April 2014 - October 2014. Research Project Manager, Oak Ridge Institute for Science Education, contracted to the FDA, Office of Generic Drugs

Scientific program management of GDUFA funded awards Program management of ~75 scientific grants and contracts Responsible for ~$40,000,000 Assisting with ANDA (generic) bioequivalence guidance and

database organization

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July 2013 - August 2014. Volunteer Research Scientist, National Cancer Institute, Frederick, MD. Gene Regulation and Chromosome Biology Laboratory

July 2010 - April 2014. Scientific Officer, Congressionally Directed Medical Research Programs (CDMRP), U.S. Army Medical Research and Materiel Command, contracted through Battelle Memorial Institute

Scientific management of ~130 grants and contracts in 19 military relevant diseases from proposal selection through budget and scientific negotiations, monitoring research progress and final study closeout

Management portfolio includes 30 clinical trials Responsible for ~$190,000,000 Lean six sigma training

Feb 2006 - June 2010. Postdoctoral Research Fellow, National Cancer Institute National Cancer Institute, Frederick, MD. Gene Regulation and Chromosome Biology Laboratory

Investigated the accuracy of gene expression using biochemical, genetic, and cell biology techniques in bacteria (E. coli) and yeast (S. cerevisiae)

Developed novel assays to study mutant enzymes (RNA polymerase) and errors during transcription

Published three journal articles on the accuracy of gene expression

Sept 2005 - Jan 2006. Postdoctoral Research Fellow, Penn State University

Aug 1999 - Sept 2005. PhD Dissertation Research, Penn State University Performed cellular, genetic, and high-throughput genomic analyses

(microarrays) after mutagenesis of transcription factors Published four journal articles Experience with native and recombinant protein purification from

bacteria, fungi, insect (SF9) and human cells (HeLa) Experience with cell physiology, immunofluorescence, protein

crosslinking

May 1997 - May 1998. Undergraduate Research Fellow, Indiana University

Plant transformation (Kentucky Bluegrass) with a biolistic particle delivery system (gene gun) to study expression of foreign genes for pesticide and herbicide resistance

Sept 1994 - May 1998. Laboratory Assistant, Indiana University

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Laboratory equipment and reagent setup for 300 undergraduate students on a weekly basis. Led a team of four laboratory assistants for three years

Supervision Experience: 2006 - 2009. National Cancer Institute, direct supervision of two

bachelors research fellows, two visiting scientists, one undergraduate scientist

2002 - 2005. Penn State University, direct supervision of four graduate and seven undergraduate students

1995 - 1998. Indiana University, led team of four Biology laboratory assistants

Professional Involvement: 2011 - 2014. Small Business Innovation Research working group,

CDMRP 2006 - 2010. Member and chair, Fellows and Young Investigators

Steering Committee, NCI. Duties included organizing career fair, organizing workshops, inviting speakers and panelists, scientific poster and talk judging (2007 - 2009)

2007 - 2010. Co-organizer, NCI Postdoc Seminar Series 2009, 2010. Invited Speaker, Middletown, MD High School Science,

Technology, Engineering and Math (STEM) Career Day 2008. NCI Outstanding Mentor Award Committee member 2002 - 2003. Graduate Student Representative, Penn State

University. Liaison for incoming Biochemistry graduate students; assisted the department with faculty and graduate student recruitment and functions

2003 - 2004. Alternative Careers in Biochemistry seminar committee, Penn State University

Awards: 2008 NCI Director’s Innovation (Career Development) Award 2009 NIH Fellows Award for Research Excellence (FARE) 2005 Paul Berg Graduate Award, Penn State 1999 - 2001 Braddock Graduate Fellowship, Penn State

Computer Skills: Extensive experience with Molecular Biology software (VectorNTI,

Gene Construction Kit, Sequencher, BLAST) Crystal structure and molecular visualization software (Pymol, VMD) Drawing and illustration software such as Adobe Photoshop,

Illustrator, Canvas Microsoft Office including microarray analysis and extensive data

management in Excel Familiar with both Mac and PC platforms

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Teaching Experience: Jan 2011 - current. Adjunct Professor, Biology, Hood College,

Frederick, MD 2000. Teaching Assistant, Microbiology, Penn State University

Publications: Irvin JD, Kireeva ML, Gotte DG, Shafer BK, Huang I, Kashlev M,

Strathern JN. (2014) A Genetic Assay for Transcription Errors Reveals Multilayer Control of RNA polymerase II Fidelity. PLOS Genetics, 10(9): e1004532, doi:10.1371.

Strathern, JN., Malagon F., Irvin, J., Gotte, D., Shafer, B., Kireeva ML., Lubkowska L., Jin DJ., and Kashlev M. (2013) The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae. J Biol Chem 288, 2689-99.

Venters BJ, Irvin JD, Gramlich P, Pugh BF. (2011) Genome-wide transcriptional dependence of conserved regions of Mot1. Molecular and Cellular Biology 31, 2253-61.

Walmacq, C., Kireeva, ML., Irvin, J., Nedialkov, Y., Lubkowska, L., Malagon, F., Strathern, JN., and Kashlev, M. (2009) Rpb9 subunit controls transcription fidelity by delaying NTP sequestration in RNA polymerase II, J Biol Chem 284, 19601-19612.

Irvin, JD, and Pugh, BF. (2006) Genome-wide transcriptional dependence on TAF1 functional domains, J Biol Chem 281, 6404-6412.

Kou, H., Irvin, JD., Huisinga, K., Mitra, M., and Pugh, BF. (2003) Structural and functional analysis of mutations along the crystallographic dimer interface of the yeast TATA binding protein, Molecular and Cellular Biology 23, 3186-3201.

Chitikila, C., Huisinga, K., Irvin, JD., Basehoar, AD., and Pugh, BF. (2002) Interplay of TBP inhibitors in global transcriptional control, Molecular Cell 10, 871-882.

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