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5941 Optical Ct., San Jose, CA 95138(408) 507-8635 | www.loopgenomics.com
©2017 ALL RIGHTS RESERVED. LOOP GENOMICS
It’s All in the Kit: LoopSeq’s simple workflow delivers powerful performance using existing sequencing infrastructure
The power behind LoopSeq stems from Loop Genomics’s proprietary technologies:
� Sample preparation, which generates sequencing-ready libraries, includes a critical barcoding step, and enables unbiased single-molecule counting
� Assembly and analysis (performed on Loop Genomics’s cloud servers), which synthesizes the short-reads into single-molecule long-reads
See how LoopSeq Complete and LoopSeq Select take the complexity (and expense) out of complex microbial samples and deliver more accurate and quantitative 16S sequencing—visit LoopGenomics.com
LoopSeqTM CompleteLoopSeqTM Select
More accurate microbial metagenomicsEasy, streamlined workflows
Harnesses your current NGS system
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Next-generation sequencing (NGS) promised to revolutionize biology, and in many ways it has. With thousands of species whose whole genome has been completely sequenced and a myriad more partially done, we seem to be swimming in more sequence information than we know how to handle. But there are a number of critically important gaps that the promise of NGS has bypassed. 16S sequencing for microbial metagenomics studies is one. This is where Loop Genomics comes in.
Leveraging your existing sequencing infrastructure, Loop Genomics improves the accuracy of 16S sequencing with our LoopSeqTM Complete and LoopSeqTM Select products. With LoopSeq Complete and LoopSeq Select you get:
16S SequencingSimpler. Better. Cost-Effective.
Enhanced performance
� More comprehensive species identification � More accurate species classification � More accurate relative species abundance
A cost-effective solution
� Uses your existing short-read sequencing infrastructure � Requires no additional hardware � Saves time and labor with a simple, streamlined workflow
Powerful technology
� Converts your existing short-read sequencer into a long-read, single-molecule counting instrument
� Smartly implements synthetic long-read approach � Increases sequencing accuracy (error rate <0.005%)
LoopSeqTM Complete and LoopSeqTM Select provide different and complementary implementations of Loop Genomics’s powerful synthetic long-read sequencing technology, but the advantages are the same. Choose the one with the workflow that works best for you, and use the other product for additional validation.
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The simple LoopSeq workflow...
PhylumClass
Order
Species
Genus
Family
Sam
ple
Clas
sific
atio
n
40 %
20 %
80 %
60 %
100 %
0 %LoopSeq(V1-V9)
Illumina(V4-V5)
Illumina(V3)
SAMPLE
BARCODING LONG-READLIBRARY
PREPARATION
SEQUENCING
ASSEMBLY & ANALYSIS
DATA
INCLUDED WITH LOOPSEQ COMPLETE
The LoopSeq Complete workflow is simple, streamlined, and leverages your current short-read sequencing
instruments. We supply the reagents for barcoding and library preparation, you handle the sequencing.
After sequencing, simply upload your raw data to our cloud-based assembly and analysis pipeline and we’ll
return assembled long-reads in FASTA and FASTQ formats, plus a report which includes abundance and
classification of each molecule sequenced.
...leads to more accurate microbial metagenomics studies
16S sequencing analysis of a complex soil
sample comparing conventional short-read
sequencing (Illumina (V4-V5) and Illumina (V3))
versus LoopSeq Complete demonstrates the
power of LoopSeq for delivering a better, more
detailed picture of a microbial community.
With LoopSeq Complete, >99% of the unique
16S molecules were classified down to the
individual species or genus level, whereas the
conventional approach led to classification of
only ~65% of unique 16S molecules.