INTRODUCTION OF THE COMPANY AND ITS PRODUCTS

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Variplast Industrial Trade and ServicesLtd. was founded in 1991. In the beginning, within the frameworksof researches and developments in che-mical industry, its activity consisted ofresolving problems related to the utiliza-tion of waste. In this scope of activity, weachieved results of great importance inthe reutilization of rubber and leatherwastes.

Our services comprisemainly investigations ofwater and waste water,giving a solution toproblems established bythese investigations andtechnical advice. At thepresent time, in compli-ance with current chal-lenges of life, our mostimportant consideredprofile that may be dee-

med as the principal one at same time,is our bioproducts, which can be appliedwith excellent results in agriculture andin all fields of cultivation, furthermoredesigning, developing and manufactu-ring of the necessary productive equip-ments.

We achieve significant results in thisfield. One of our products is Bioplasma:

a natural fertilizer prepared of algae thatare nutritious for plants, and stimulatestheir development, and the productiveequipment of this product, furthermore,a microbiological preparation calledBioNitroPhos, which increases soil ferti-lity. Both products can be applied withexcellent results either in traditional or inbiocultivation.

Tablets and capsules utilising the physio-logical effects of algae, garlic and gelatinand conditioning human and animalorganisms, are classed among our veryimportant products. Besides domesticmarketing, we also export these pro-ducts and equipments. We have set upthree Bioplasma productive enterprisesin Hungary and two in Sweden. But suchplants are operating also in Spain andAustralia.We export basic material necessary forthe manufacturing of Bioplasma fromHungary to other plants of Europe.

Our products hold the trademark ofBioculture Association and the trade-mark of KRAV in Sweden. The producti-on in Hungary is inspected byBiokontroll Hungária Kht.

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It is known that the emmission related to the spreading of intensive agricultural practices, trafficand extensive increase of industrial production has caused adverse changes in our environmentand plant cultivation. This change can be measured as an unfavourable change in the chemical compositi-on and microelement content of soil, surface waters, flora, fauna and even of foods. It is characterized by an accumulation of toxic microelements and a decrease in con-centration of essential microelements. Its consequence is an adverse physiological effect and promotion of disease develop-ment in humans and animals at the end of the food chain. That is why nowadays agriculture undergoes a change throughout the world. Themost important task is the formation of a social conception, which prohibits the dest-ruction of wildlife. In agriculture, this may be realized by establishing new, up-to-datecultivation methods and by applying environment-friendly nutrients. Forward-thin-king people, having recognized their responsibility, are looking for new ways andmethods for recovery and rehabilitation, in order to form our present and joint futurewith Earth conscientiously. Application of Bioplasma and BioNitroPhos encourages the realization of these pur-poses in the scope of plant cultivation. Applying bionutrients in ecological farming is a future determining tool, and appliedenvironment protection at the same time.According to the expectations of the European Union, in our own interest we have tostop pollution of the soil and environment in the course of plant cultivation, bymeans of manufacturing products without any harmful effects on living organisms,and which do not contain any toxic material.

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NEW WAYS IN THE ENVIRONMENT-FRI-ENDLY PLANT CULTIVATIONBIOPLASMA

Bioplasma is a bionutrient of complex effectsdeveloped for agricultural plant cultivation. With starting its production, we have made ava-ilable a natural substance for agriculturists,which serves and stimulates the developmentand growth of plants in a higher degree compa-red with previous results. Bioplasma is produced by the propagation ofalgae on a nutrient mixture of special composi-tion with a so-called photobio-fermentationmethod. As the number of algae increases,more and more nutrients undergo a transfor-mation into a substance of a favourable chemi-cal structure for plants. Plant nutrients producedin this way contain algae in an immediatelyabsorbable and incorporatable form and in anoptimal ratio at the same time, and all elementsand substances stimulating the development ofplants, macro-, meso- and microelements, furt-hermore vitamins and amino-acids,which are needed for the develop-ment of plants. Therefore,Bioplasma can be applied in all kinds ofplant culture, both in conventional andbiocultivation.Bioplasma has a complex effect on thejoint system of soil and plants. One ofthe reasons thereof lies in its compositi-on. Through their root system plants caneasily absorb the macroelements dissol-ved in Bioplasma and the meso- and mic-roelements of an optimal concentrationrange, which facilitate physiological andbiochemical processes and the balanced

development of plants.Nutrients in alga cells con-tained by Bioplasma arepresent in a very highconcentration, and by cominginto contact with plants of lownutritient concentration, nutri-ents flow into the plant cells bythe effect of diffusion (differencein concentrations). Cultivation investigations withBioplasma conducted in therecent years in Hungary, Europeand several countries of theworld (eg. Italy, Sweden,Denmark, Slovakia, Turkey,Finnland etc.) have clearly pro-ven that Bioplasma is suitable for

chemical-free plant cultivation.

Crop results achieved with different plants (eg.cereals, tomato, capsicum, cucumber, fruits andflowers, etc.) has also confirmed that usual ave-rage yields can be exceeded. In addition, thepulp, colour, taste and shape of plants cultiva-ted with bioplasma were also much more favo-rable. Treated plants are extremely resistant to disea-ses and pests. In several cases, ill plants started to developagain after having treated them with Bioplasma.

EFFECT ON SOIL AND PLANTS

Plants absorb nutrients from the dissolvedphase of Bioplasma sprinkled on the soil thro-ugh their secondary rootbranch. After havingbeen transformed by the effects of favourableconditions (sunshine, inorganic nutrient residu-es) algae begin to propagate. In case of decay,they serve as nutriments for microorganismspresent in the soil. It improves soil structure and water balance and

stimulates soil life. Abundant nutrients of algae

are transferred into the plantsthrough their leaves facilita-ted by diffusion.

ADVANTAGESUpon its effects, odds of spro-uting, taking root and gro-wing increase, the greenweight of roots and plantsrises, blooming takes placeearlier, and crop adhesion ismore favourable. Yield quan-

tity increases. It facilitates theproduction of flavour andaroma substances in the cropsand prolongs the period ofpreservation. It increases the

resistance of plants to diseasesand helps to survive rigours of

weather. It can be spred with irrigation systemsbeing currently in use. Soil and ground waterpollution decreases with its application.

PRODUCTBioplasma is available on the market in form ofa green coloured liquid of a pleasant odour. It isa concentrate, which may be used after dilutionwith water to a concentration specified in theinstructions for use or according to the expertadvice.It is a non-toxic, inflammable and non-explosivematerial, the use whereof does not involve anyhazard to humans or animals. Waiting period

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after use: zero day. Bioplasma is a 100% utili-zable, environment-friendly plant nutrient.

WHERE CAN IT BE APPLIED• In field vegetable growing • In vegetable and fruit cultivation • In transplant growing • In greenhouses and foil tents• In the cultivation of flowers and

ornamental plants • In biocultivation • In viticulture and fruit cultivation• In forestry• Where irrigation is carried out • In hobbi gardens, in case of small-scale

producersAs it clearly appears from the above, Bioplasmahas an extremely wide range of application, sothere is hardly any small-scale or large-scaleproducer, who could not make a benefit fromthe advantageous effects of Bioplasma.

RECOMMENDED USEBioplasma is suitable either for foliar or soil fer-tilization in plant cultures of all kind. It shall beused after diluting with water, in a concentrati-on depending on the growth stage of the plantand its nutrient requirements. As a general ruleof use, following shall be taken into considerati-on: it shall be used in 80-120-fold dilution asfoliar fertilizer, and in 40-60-fold dilution as soilfertilizer, depending on plant species. Please, donot hesitate to contact the manufacturer foradvice. Bioplasma may be spread together withother substances, for example with plant pro-tection chemicals, in the manner that first theadequate dilution of the plant protection che-mical shall be prepared and Bioplasma addedonly after that by continous mixing and thenthe substance shall be immediately sprayed out.

Please, find below some examples for com-mon methods of use and the obtainedresults.

CEREALS

The product was applied as a foliar fertilizer inthe cultivation of corns in the ear, maize andsunflower. In case of corns in the ear, it wassprayed out as a foliar fertilizer mixed withother plant protection chemicals at the time offoliation and earing. The plants were more resis-tant to drought and fungal diseases. Witheringand partly desiccated cultures could be savedwith two to three plants spraying. Yield averagewas 10-20% higher. Proportion of gluten was32-34. In case of sunflower, pumpkin and shell-

less pumpkin yield average was 30-50% higher,depending on environmental conditions. Plantspraying was carried out by the traditional met-hod or from helicopter. Concentrated Bioplasmawas applied three times in a quantity of 8 litresper hectare, but other producers applied it in aschedule of two times 8 litres per hectare and1-10 litres per hectare respectively. It could beobserved that by increasing the number of app-lication, plant cultivation became more produc-tive. By rape and mustard similarly good results wereachieved with the application of Bioplasmathree times in a quantity of 8 litres per hectare.

VEGETABLES

Bioplasma can be applied in transplant growingas a nutrient solution or foliar fertilizer.According to the data of KITE, capsicum bro-ught a yield in the lack offostering soil, cultivatedonly on Bioplasma soluti-on. Cabbage transplantsgrown on soil block alsodeveloped excellently.

Experiences are goodboth with plants grownunder foil and in openfield. If applyingBioplasma for tomatogrown under foil, lessother plant protectionchemicals are needed, andseveral producers havereported that they totally ceased to apply them. Tomatos had a thick stem, and were healthy.Experiments in field vegetable production con-ducted by SZIE showed that ripening of indust-rial tomato is more healthy and begins 5-7 daysearlier. It surpassed the control plants both in quantityand quality.

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As for capsicum, the observations are the same.We achieved 15-20% higher yield quantity, agreen foliage and healthy fruits without app-lying any chemicals. In case of capsicum, wehave very good experiences with Bioplasmasupplemented with Ca.Diseases, such as brown rot, etc. did not occur.We have received positive feedbacks with spicepeppers too. If applied for cucumbers, only first class fruitswere produced. Having applied for potatoes as a foliar fertilizer(3x8 L/hectare), we obtained 20-30% higheryield average and a strong green foliage. If applied for salad crops, cabbage, chinese cab-bage etc. plants had wonderful succulent leaves.

GRAPE VINE:

If spreading it into the root zone, it is extremelyeffective in vine-stock planting or replacement.According to the unanimous opinion of produ-cers, nursery stocks took better root and losswas minimal even if the weather was dry. Plants on treated areas are characterized with astrong sprouting. Stock plants are more freezeresistant and more safely pass the winter. We have received back reports from severalproducers that new plantings became yieldingone year earlier. /Bioplasma was spread into the

soil in a concentration of1-2%/Nurseries have alsoreported good results inplant stock growing.After a cold winter, ifgrape cultures sufferfrost damages, they canbe excellently restoredand freshened. If applying as foliar ferti-lizer, BP is also very effi-

cient in dry, rainless periods. Its effects can be almost immediately observed.Shrivelling of the leaves stops. Plants are characterized with a strong sproutingin spring, and with an excellent adhesion whenblossoming. Producers have also reported anincreased resistance to fungal diseases. In the average of several years a surplus yieldquantity of 10-15% can be expected in treatedvinelands, accomplished by a higher sugar deg-ree. It shall be applied in a schedule of 3 x 8 L/ha bygeneral use, but experiences show thatBioplasma is used to appy by every spaying,however, in a reduced dose.

The amount and frequency of Bioplasma appli-cation strongly depends on the quantity of pre-cipitation.

FRUITS:

It is spread into the soil by stock planting, simi-larly to vine grape, but immersion intoBioplasma solution of 3-5% before stock plan-ting is also commonly applied. Bioplasma pro-ved to be valuable by all kinds of fruit, however,as many kinds of fruit, as many methods ofapplication as a foliar fertilizer. It is applied in an amount of 3x8 L/ha in case ofstone fruits and berries, however, for apples andpears, which usually need much more plantprotection, it is used as much as 5 times. Results:Plants become more resistant not only to fungaldiseases but also to pests eg. rust mites.Adhesions are good, in case of apples there areno colouration problems, and Bioplasma supp-lemented with calcium bears a great importan-ce in the prolongation of apple preservationperiod. In general, producers reported on per-fect and healthy fruit crops. In fruit cultures it became common thatBioplasma is applied also by flushing spraying/before sprouting/ in a concentration of 1%.

In case of berries, ripening shortens by 3-5 days.Experiences are very favourable with strawberri-es /Fragaria/.A surplus yield quantity of 15-25% can beexpected /dropping irrigation/ and the qualityof strawberries is also very advantageouslyaffected, its preservation period lengthens.

BIONITROPHOS

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BIONITROPHOS

BioNitroPhos is a microbiological preparationintended to increase soil fertility, facilitate thedevelopment of normal soil conditions, increaseyield quantity and promote the utilization ofstubble fields. It contains apathogen fungi, nit-rogen-fixing and phosphorus-mobilizing bacte-ria, polysaccharids, polipeptides, enzymes andother additives. It is a known fact that microor-ganisms present in the soil play a prominentrole in the decomposition of organic substan-ces, mobilization of minerals, forming a thickhumus-layer, in the continuous nutrient supplyof plants and in the inhibition of phytopatho-gens. In an area of one hectare, soil contains 3-15 tons of microorganisms, depending on theconditions. The three most important kinds ofmicroorganisms can be found in the followingquantity in an are of one hectare.

Bacteriaapprox. 3t/haFungi approx. 6-8t/haAlgae approx. 0.03-3t/ha

Soil serves as a natural medium for plants andmicroorganisms. Under natural circumstancesthe existance of the one determines the exis-tance of the other. In the past decades we rest-ricted only to the replacement of soil nutrients,without having considered that for a succesfulplant cultivation quantities of microorganismsare also necessary. With its activity aimed at increasing yield results(soil tillage, nutrient replacement, plant protec-tion), mankind has completely disturbed thebalance, which had been established for mille-nia. The environmental burdening of soil hasincreased and hereby the number and compo-sition of soil bacteria changed. Agricultural areas have became acidified, watershave been contaminated with nitrates and inplants such substances have appeared, whichexert unfavourable effects on wildlife (heavymetals, residues of plant protection chemicalsand their metabolits). The direction of future is the restoration of soilconditions by applying such environment-fri-endly substances, which increase soil fertilityand at the same time, do not endanger theenvironment and reconstruct the originalecosystem. Natural soil is a system of complex substancesof different quality and physical condition, it isa nutrient medium for plants consisting of 4mean components:

• Organic substances• Inorganic components (minerals)• Soil solution (groundwater)

• Gases (air, carbon-dioxide) In course of the top-soil formation, complexphysical-biochemical processes take place withthe participation of soil constituting substances.From the aspect of plant cultivation, the mostimportant conditions to these processes are thechemical elements and the energy, carbon, nit-rogen and phosphorus cycle, which is essentialto the life functions of cells. BioNitroPhos exertsan effect on the different soil constitutingsystems, in order that natural processes influen-ce the cycle of nutrients, the fertility, the energyprocesses and the water balance properties ofthe soil.

Microorganisms present in BioNitroPhos provi-de decomposition of organic soil substances,introduction of nitrogen into the soil and mobi-lization of phosphorus. Ouraim is to promote the formati-on and spreading of environt-mental-friendly farmingsystems besides increasingyield results and improvingcrop quality with regard to theexpectations and regulationsof the EU.BioNitroPhos, as a bioproduct,possesses the certification ofBioculture Association and itsproduction is inspected byBiokontroll Hungária Kht.Soil carbon source is producedpredominantly by the utilizati-on of sun energy, by pho-tosynthesis. 50-90% of carbonassimilated hereby ends up inthe soil, gets mineralized andmoves to the humus-layer. Humus is the endproduct of organic decomposition. It consists ofvery small particles. This formation containsnutrients in the root zone, which are importantfor plants. Nitrogen, an important elementnecessary for the growth of plants can be intro-duced into to soil by means of artificial or natu-ral fertilizers. Nitrogen source supplied by artifi-cial fertilizers can not be entirely utilized, as apart of it gets washed out and another partescapes. The decomposition product of organicsubstances of animal or plant origin introducedinto the soil, is ammonia. Green plants areunable to take up the nitrogen of ammonia. Itis transformed into nitrites and nitrates by nitri-fying bacteria. These ions can be directly utili-zed by plants. Air is an inexhaustible nitrogensource with a nitrogen content of 78v/v%.Plants are unable to take up and assimiliate thenitrogen of air. We can provide a continuous

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nitrogen-supply for our plants with the help ofmicroorganisms present in BioNitroPhos. Theseare nitrogen-fixing microorganisms and micro-organisms changing the structure of soil ammo-nia. Hereby 40-60% of artificial fertilizers canbe substituted. Soil phosphorus can be found inbound form in organic and inorganic substan-ces. It is mostly insoluble or hardly soluble, inan unabsorbable form for plants. By introducingphosphorus-mobilizing strains present inBioNitroPhos into the soil, after their propagati-on, complex phophorus-compounds normallyunavailable for plants become soluble.BioNitroPhos is a non-toxic, to humans, animalsand to the environment non-hazardous micro-biological concentrate of natural origin. Storage rot causing fungi present inBioNitroPhos are able to produce enzyimes (eg.ligninolytic enzymes, cellulase, hemicellulase,ligninase, lignin-peroxidase, laccase, etc.) neces-sary to degrade structure substances of plants(lignine, cellulose, hemicellulose). One of thefungal strains possesses a significant lignin-deg-rading activity, whereas the other one decom-poses lignin and cellulose at the same rate. These fungi considerably facilitate the complexdegradation of hemicellulose and provide anexcellent carbon and energy source for cultiva-ted plants and soil bacteria fixing molecular nit-rogen and mobilizing phosphorus. Fungi present in the preparation are apathogenand do not infect living plants. One of the nit-rogen-fixing bacterium strains of BioNitroPhoslives normally in the soil. This microorganism is able to fix themolecularnitrogen of air, and to reduce it into ammoniawith the help of an electron mediator moleculeand a nitrogenase enzyme. Ammonia is transformed by nitrifying bacteria

into nitrites and nitrates, which is easily absor-bable for plants. It is able to fix as much as 20-30 kg nitrogenper hectare from the air during one plant culti-vation cycle. The other nitrogen-fixing bacteri-um strain is able to fix nitrogen in the proximityof another pre-organism, eg. root-system of aplant. In addition to providing the necessarynitrogen for the growth of plants, its importan-

ce lies also in the fact that it is able to producehormones, substances stimulating the growth ofplants. It is able to fix as much as 40-60 kg nitrogenper hectare during one plant cultivation cycle.The phosphorus-mobilizing bacterium strain ofBioNitroPhos plays a role in iron absorbation atthe same time. Under natural conditions, phosphorus can befound in organic and inorganic compounds ofthe soil. These phosphorus-compounds are

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hardly soluble or insoluble in water. Their avai-lability for plants is minimal. By introducing andpropagating phosphorus-mobilizing microoga-nisms into the soil, complex phosphorus-com-pounds normally not available for plants beco-me soluble.

In addition, the persence of phosphorus-mobili-zing microorganisms has an important role, asthey are able to capture iron also if low ironconcentration is present, and to form so-calledsideophores. The result thereof is an inhibition of harmfulbacteria and fungi growing in the rhizosphereeg. propagation inhibition of the potato patho-gen Ervinia caratovora by means of iron-defici-ency, or growth stimulation of potato, sugarbeet and radish on an iron deficient soil bydelivering captured iron directly for the cultiva-ted plants.

It has a great importance from the aspect ofplant cultivation that phosphorus-mobilizingbacteria are able to synthetize hormones stimu-lating the development of plants (eg. gibberelinacid). Advantages of the application ofBioNitroPhos

Effects exerted by fungi• Facilitates quick decomposition of

organic substances and formation of humus

• Supersedes stubble burning and hereby stops the related environment destruction and pollution

• Enables the decompositon of lignin and cellulose containing organic substances ploughed into the soil

Effects exerted by bacteria • Reduces the needed amount of chemical

fertilizers to an economic extent and at the same time, increases their efficiency and decreases the loss

• Inhibits the propagation of other pathogen bacteria and fungi growing in the rhizosphere

• By fixing nitrogen from the environment (air), reduces the amount of other substances of nitrogen content necessary to introduce into the soil

Joint effects • Improves soil structure and water balance

and hereby reduces soil crustation • Favourably influences the pollution of

surface and groundwaters • Favourable influences the quantity and

structure of the root system• Under appropriate conditions, in addition

to improving fruit quality, it increases crop capacity

Recommended also for biocultivation

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RECOMMENDED USE

BioNitroPhos increases soil fertility, and can beapplied for all kinds of plant culture. It can be applied:

• In field vegetable production • For treating meadow-lands, pastures,

grasslands • In horticulture • In viticulture • In tree and forest plantation • In the cultivation of flowers and

ornamental plants • In top-dressing

Conditions of spreading: • Windless weather • Preferably wet soil • Temperature between 10-25 °C

Quick and favourable results can be expected, ifthe soil meets the following requirements:

• pH value is between 6-7.5• Organic soil content exceeds 1.5%• Carbon-nitrogen ratio (C/N) is 12:25

If the soil does not meet these requirements, itis advisable to perform soil conditioning first.BioNitroPhos may also be applied in case theorganic soil content does not reach the 1.5%. Inthis case 35-40 kg substance of nitrogen con-tent shall be spread into the soil, per hectare.

Time of spreding: • 2-3 weeks previous to sowing, planting or

transplant planting• In case of grape vine, 2-3 weeks previous

to sprouting • In case of fruit cultivation, after fruit

harvest• 2 weeks before tree or forest plantation • 2 weeks before planting flowers or

ornamental plants Until restoration of normal soil conditions regu-lar use is extremely important, thereafter it isrecommended.

Manner of spreading

The water dilution of BioNitroPhos, or its soluti-on prepared with 35-40 kg inorganic substanceof nitrogen content (if it is needed becauseorganic soil content does not reach the 1.5%)shall be evenly spread into the soil by

• Irrigation or• Spraying

and superficially ploughed into the soil. BioNitroPhos may be spread into the soil at anytime, however, expected results will manifestonly after the propagation of microorganisms.

Storage conditions at 4-5°C 6-8 weeksat 6-8°C 2-3 weeksat 10-12°C 6-8 daysThe product is a bacteria and fungi concentratecontaining living organisms in a great number,so its preservation period depends primarily onthe temperature. It can be stored together withother materials in cellars, as it is not dangerousto the environment. Because of its high organic and microorganismcontent, temperature of BioNitroPhos may notpermanently exceed 15°C during transport andstorage.

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Recommended quantities for application

By the application of BioNitroPhos followingshall be taken into consideration:

• PH value of the soil • Organic soil content • Amount of precipitation • Soil type• Growing season of the plant

Depending on soil pH: pH=6.5-7.5 10L/haIt soil pH deviates in either direction from thisrange, quantity shall be increased by 1 litre per0.5 pH unit. Depending on organic soil content: 2%, 12L/haIf organic soil content rises by 1%, 1 litre lessmaterial shall be spread. Depending on the amount of precipitation: onwet areas: 10 L/ha On rainless areas: 12 L/haDepending on soil type

• Clay soil 11 L/ha• Sandy soil 13 L/ha• Adobe soil 10 L/ha

Depending on the plant’s vegetation period:In case of cultures of a longer vegetation peri-od, it advisable to apply the product also fortop-dressing or side-dressing.BioNitroPhos quantity recommended for appli-cation shall be diluted with water to 300-500litres and spread into the soil.

Experiences with the application of BIONIT-ROPHOS

According to the experiences of users, sprea-ding with BNP technology shortens the periodof time necessary to the decomposition ofstubbles, stem and root residues. It also showed

to be effective in the decomposition of vines-hoot and reed residues.Considerable sum of money can be saved bythe users, because conventional nitrogenousfertilizers are not needed any more to thedecomposition of stem residues.

If applied for stubbles after reaping or for sunf-lowers or maize in autumn, it promotes thedevelopment of appropriate soil quality neces-sary to the preparation of seed-beds. In spring, sreading of BNP takes place simulta-neously with soil preparation, so the costs ofspreading can be minimalized.

On the basis of experiences obtained duringyears, due to the favourable effects of BNP510qmore yield quantity can be achieved bycereals, but in an extremely rainless period oftime a yield increase of even 70-80% could bemeasured by sunflowers, compared to the cont-rol plants. Besides cereals, BNP produced a significantlyhigh yield average in case of sugar beet andsoy bean. These plants’ average of 35-41 q/harose by far above the average of untreatedareas, which amounted to 32 q/ha.

In addition to surplus yield, producers alwaysemphasise the improvement of soil quality,which makes further soil farming easier andsaves work.

If applying for field vegetable production,experiences are very favourable with fruit culti-vation, in particular with berries.

H-1028 Budapest, Gyopár u. 14.

Telefon/fax: (+36 1) 397 6089 • Telefon: (+36 1) 391 6737