IntelliPlex 1000 Resource... · 2020. 11. 5. · 1. Click ADD PROCESS 2. Click Select 3. Choose the process from the drop down menu. (The following slides will explain each process

IntelliPlex 1000

Copyright 2019 PlexBio, Co., Ltd.

Custom Program_P-5_V03

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Custom Program

Software version 2.5.0 and newer

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Click Custom Program Select rows Six custom program can be saved (Program 1 to 6). Select one to run, create or edit a program.

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Adding a new process

Five process options

Incubation

Washing

SA-PE Reaction

Disperse

Pause

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1

2

3

4

5 6

Adding a new process

1. Click ADD PROCESS

2. Click Select

3. Choose the process from the drop down

menu. (The following slides will explain each

process in more detail.)

4. Once a process has been adjusted, click SAVE

to save changes to the program

5. Click the forward arrow to run the program

6. Click the back arrow to adjust settings in a

previously added process

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Incubation

Edit incubating temperature, heating

period and other parameters

Temperature range is 20-60ºC

• The IntelliPlex 1000 does not contain a cooling

system, so the low end of the temperature range

will be dependent upon room temperature

(RT+5˚C)

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Incubation

Close lid while heating (suggested)

• Closes heating lid to cover the microplate

Shake before reaching temp (not

suggested)

• The microplate will start to shake before the

desired temperature is reached

• Timer starts once the microplate shakes, not

once the desired temperature is reached

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Washing

Edit the Wash Cycle Number (3 cycles is

suggested)

Washing Volume o Volume of wash buffer dispensed into wells

during each wash cycle (ELISA = 200μL)

Last Dispense Volume o Dispenses 150 μL of wash buffer into wells after

the last wash cycle to ensure appropriate buffer volume for analysis

o Do not select this option if nest step is SA-PE labeling as it dilutes the SA-PE solution.

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Washing

Magnetic

• Activates magnetic function during wash

cycles

• Must be clicked ON ■ for πCode assays.

Clean tip

• Tips will be cleaned after each row before

proceeding to next row (only required for the 1st

washing process for most assays)

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SA-PE Reaction

Includes all steps for fluorescent labeling:

SA-PE dispense (50 µ L/well), incubation, 4 wash

cycles, and 10 sec. shaking to spread πCode

evenly. (All parameters except incubation period are

fixed.)

Heating period

• Adjustable from 1s to ~24hr (Suggested

heating period is 10 min)

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Pause

Allows for a pause between processes

It may be needed to add additional solution to a

sample manually. To do so, take 96 well plate out

of the IntelliPlex 1000.

Before continuing with the program, ensure that

96 well plate has been positioned correctly in

the IntelliPlex 1000

Continue with the next process in the program

by clicking “OK”

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Disperse

Shake and pipet the components in wells.

The tips will conduct self-clean among

rows.

• This is the last step of a program. It spreads

πCode MicroDiscs evenly before analysis.

• Not an adjustable parameter.

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Preparing to run program

Check and save the program before proceeding

to next step

Click to edit the program or rows if needed

Ensure there is sufficient wash buffer and

ddH2O for running the assay

Make sure the air vents of buffer bottles are

loose

Remember to add SAPE in V tray. See next page

for SAPE usage calculation.

Click START to run the program.

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SA-PE usage calculation Dead volume: 500μL

Minimum dispense: 400μL for one row

Example:

For 3 rows reaction… 400 µ L x 3 rows + 500 µ L = 1.7mL (at least)

* Add 300 µ L more if running more than 6

rows

Front

Back

V Tray (SA-PE Tank)

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Oct.-2019 - Page 14

Thank You

PlexBio 6F-1, No. 351, Yangguang St. Neihu District, Taipei City 11491, Taiwan +866-2-2627-5878 PlexBio.com [email protected]

Documents

IntelliPlex 1000 Resource... · 2020. 11. 5. · 1. Click ADD PROCESS 2. Click Select 3. Choose the process from the drop down menu. (The following slides will explain each process