INICIO ANAIS INGLES 2005 - Inicial — UFRGS Ingles - SBTE-2005 - Indice... · Marco Antonio Alvarenga Presidente 2004-2005. II Acta Scientiae Veterinariae 33(Supplement 1): 2005

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Acta Scientiae Veterinariae 33(Supplement 1): 2005

Presidente Speak

Welcome to Angra dos Reis!

We are in one of the most beautiful places that the nature have to offer on the Brazilian cost side,the combination of mountain and ocean will created a perfect atmosphere to celebrated the 20th birthdayof our Society.

The program will permit the SBTE member to enjoy, at least a few, of this fantastic place. Pleasuremoments during the SBTE meetings are, in my opinion, very productive. Hard to say how many scientificcooperation’s and partners started during our meetings!

During the event we will remember the evolution and good moments of our Society. The SBTEhistory was carefully reviewed by our colleague Mara Rubin, in a beautiful article that is published onthis proceedings..

Mara is one of the SBTE pioneers, she was also our second President and is strongly connectedwith our Society during the last 20 years.

The history of our Society is the history of Embryo Transfer in Brazil. You will realize howimportant were and are our SBTE members on the development and diffusion of technologies appliedto embryos. Don’t forget to read this article!

My congratulations to all the SBTE pioneers and special the members that followed all steps ofSBTE development. They will be our honored.

SBTE had in 1985 around 40 members today we are around 600.Our size is also a picture ofEmbryo Transfer in Brazil, in 1992 15 thousand ET were performed yearly today we performed closeto 150 thousand ET yearly. Our Society is big today in several aspects: we are today the BrazilianSociety with the largest number of Veterinarians and our meeting the second of the world on EmbryoTechnology area on number of papers and participants.

This year we received a record number of papers to be published (300), it is the double than theaverage of publications on our last 3 meetings and is similar with the number of papers that arepublished at IETS.

As SBTE formal President I would like to thank my wife Fernanda, that is our Scientific Director,for her care and dedication, coordinating the selection and publication of all lectures and abstracts. Sheexpended four months working full time to SBTE .

I am grateful to many people for their assistance. I would like to thank the speakers and reviewers,for the time that they expended.

We also want to congratulate all 24 private and 3 governmental sponsors. Your support is therecognizing of the potential and competence of our members.

I would like on this opportunity also to specially thank our 15 partners companies and our secretaryAlex for their daily help. Joao Henrique and Meira for their work with the society newspaper andtreasure, respectively.

My sincerely wishes of success to the next Board of Directors of SBTE. It was intense, sometimes hard, but a great honor to administrated our Society.

Marco Antonio AlvarengaPresidente 2004-2005

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ORGANIZING COMMITTEE OF THE XIX ANNUAL CONFERENCE OF THE SBTEAugust 25 to 28, 2005

EXECUTIVE BOARD

Marco Antonio Alvarenga (President)João Henrique Moreira Viana (Vice-President)

José Buratini Jr. (1° Secretary)Flavio Meireles (2° Secretary)

Cezinande de Meira (1° Treasurer)Carlos Antonio Carvalho Fernandes (2° Treasurer)

Fernanda da Cruz Landim e Alvarenga (President of the Scientific Commission)

SCIENTIFIC BOARD

EDITORS OF THE PROCEEDINGS:

Fernanda da Cruz Landim e AlvarengaMarco Antonio Alvarenga

DESKTOP PUBLISHINGJosé Luis Barbosa de Souza

Fernanda da C. Landim Alvarenga – President CCAlberto GusmãoAlceu MezzaliraAlicio Martins Jr.Anneliese de Souza TraldiCarlos Eurico dos Santos FernandesCarlos Antonio Carvalho FernandesCésar Roberto EsperCezinande de MeiraCiro Moraes BarrosEduardo Paulino da CostaElen Silvia C. Siquiera-PylesFabiana Ferreira de SouzaFrederico Ozanan PapaGustavo F. CarneiroJoão Carlos Pinheiro FerreiraJoão Henrique Moreira VianaJoaquim Mansano GarciaJosé Antonio Visintin

José Buratini Jr.José Luis Moraes VasconcelosJosé Ricardo FigueiredoMara Iolanda Batistella RubinMarcelo NogueiraMarcelo Marcondes SenedaMarco Antônio AlvarengaMarco Antonio Coutinho da SilvaMaria Angélica MiglinoMaria Denise LopesMario BinelliNereu C. PrestesPéricles A. HassunPietro Sampaio BaruselliRoberto SartoriRodolfo RumpfRubens Paes de ArrudaSony Dimas BicudoVera Fernanda Hossepian de Lima

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SBTE IS GRATEFULL FOR THE SUPPORT:

OF THE FOLLOWING GOVERNAMENTAL INSTITUTIONS,

CNPqCAPESUNESP

Conselho Federal de Medicina Veterinária

OF THE SBTE PARTNERS

AKZO NOBEL LTDA -DIVISAO INTERVETBIOCOM INDUSTRIA COMERC E SERVIC LTDA

BIOGENESIS DO BRASIL LTDACOOPERS DO BRASIL LTDA

EPPENDORF DO BRASIL LTDAFELDMAN WILD LEITZ IMPORT. E EXPORT. LTDA

FORT DODGE SAUDE ANIMAL LTDAGENEALOGICA LTDA

LABORATORIOS HERTAPE CALIER DO BRASIL LTDALABORATORIOS PFIZER LTDA

MINITUB DO BRASIL LTDANUTRICELL NUTRIENTES CELULARES LTDASPECTRUN BIO ENG MED HOSPITALAR LTDA

TECGENETECNOPEC LTDA

TRANSFIX TRANSPLANTES DE EMBRIÕES LTDAVITROGEN PESQ DESENV BIOTEC DA REP SC LTDA

AND OTHER COMPANIES

BIOEMBRYO BIOTECHNOLOGIA DA REPRODUÇÃO LTDACONTROLMAX - CONSULTORIA E SISTEMAS LTDA

CULTILAB MATER P/ CULT DE CELULAS LTDAINGÁMED

IVP - INSTRUM VETERINARIOS E PALHETAS DO BRASIL LTDAOURO FINO PRODUTOS VETERINÁRIOS

SANTA LYDIA LTDATECNAL EQUIPAMENTOS PARA LABORATORIO LTD

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Program: XIX Anual Meeting of the SBTE

I. SHORT COURSEAugust, 25, 2005 (3 hours)

1. Short course on ovum pick-up and oocyte manipulation at field conditions in cowsPeter Bols – BélgicaJo Leroy - BélgicaJoão H. Moreira Viana – Embrapa, Coronel Pacheco

2. Short course on new strategies on cooling and freezing equine semenEdward Squires – CSU – USAFrederico Ozanan Papa – UNESP - Botucatu

3. Short course on embryo transfer in small ruminantsAlberto Gusmão - UFBA

II. SBTE Anual Meeting

August, 25, 200501:00 to 06:00 PM - Registration07:00 to 08:30 PM - Opening Session

Twenty Years History Of The Brazilian Embryo Technology Society (1985-2005) – Dra Mara Iolanda Batistela Rubin – UFSM – RS

08:30 PM - Opening Cocktail

August, 26, 200508:00 to 09:00 AM - Advances In The Understanding Of Follicle Developmental Physiology Dr.

José Buratini Jr. - UNESP - SP09:00 to 09:30 AM - Oral Presentation

09:30 to 11:00 AM - Coffe Break and Poster‘s Session A11:00 to 04:00 PM - Lunch and free time

04:00 to 05:00 PM - Perspectives On The Use Of Biotechnology In Equine Reproduction – Dr.Edward Squires – CSU - USA

05:00 to 05:30 PM - Oral Presentation

05:30 to 06:00 PM - Coffee Break06:00 to 07:00 PM - Evolution Of Transgenic Farm Animals - Dr. Heiner Niemann - FAL -

Alemanha07:00 to 07:30 PM - Oral Presentation

August, 27, 200508:00 to 09:00 AM - Technical And Biological Aspects Of Ultrasound-Guided Transvaginal

Oocyte Retrieval In The Cow: An Overview - Dr. Peter Bols – Bélgica09:00 to 09:30 AM - Oral Presentation

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09:30 to 10:00 AM - Coffee Break10:00 to 11:30 AM - STUDENTS COMPETITION11:30 to 03:00 PM - Lunch and free time

03:00 to 05:00 PM - Work Shops1 – Control of genic expression on IVP embryos2 – Practical aspects on AI and ET in Bovine3 – Practical aspects on AI and ET in Equine4 – Practical aspects on AI and ET in small ruminants

05:00 to 06:30 PM - Coffee Break and Poster‘s Session B

06:30 to 07:30 PM - SBTE Associate’s Assembly08:30 PM - Closing Diner and Students Competition Results

August, 28, 200508:30 to 09:30 AM - In Vitro Production Of Bovine Embryos An Obstetric Overview Of Problems

And Challenges - Dr. Nereu Carlos Prestes – UNESP - São Paulo09:30 to 10:00 AM - Oral Presentation

10:00 to 10:30 AM - Coffee Breack10:30 to 12:00 AM - ROUND TABLE DISCUSSION: Problems related to commercial IVP

embryosMichele Watanabe – Vitrogen - SPLuiz Mauro V. Queiroz - BIO – Biotecnologia em Reprodução Animal Ltda -DFWilson Pardini Saliba – Cenatte – MG

12:00 to 12:30 PM - Closing Session

III. Work Shop:

1- Control of genic expression on IVP embryosHeiner Niemann – AlemanhaFlavio Meireles – USP/PirassunungaMatt Wheeler - EUA

2- Practical aspects on AI and ET in BovineEd Hoffman Madureira – USP/PirassunungaRubens Paes de Arruda – USP/PirassunungaPietro Barusseli – USP/SP

3- Practical aspects on AI and ET in EquineAlberto Farinasso – Central Unicórnio/Brasília, DFJose Neves Neto – Fleury Reprodução Eqüina/S. J. Rio Pardo, SP

4– Practical aspects on AI and ET in small ruminantsSony Dimas Bicudo – UNESPAlberto Gusmão – UFBAMaico H. Barbosa – UFRPE - PE

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CONTENTSActa Scientiae Veterinariae, 33(Supplement 1): 2005

President Letter .................................................................................................................................. IAcknolowdgments ............................................................................................................................ IIIProgram ............................................................................................................................................ IV

SHORT COURSE

Short course on ovum pick-up and oocyte manipulation at field conditions in cows

BIOLOGIC VARIABLES ASSOCIATED WITH CUMMULUS OOCYTE COMPLEX RECOVERYUSING FOLLICULAR ASPIRATION. Viana, J.H.M.; Bols, P.E.J. ................................................................ 1

OVUM PICK-UP AS THE IDEAL TECHNIQUE TO STUDY THE INTRAFOLLICULARENVIRONMENT AND OOCYTE QUALITY IN HIGH-YIELDING DAIRY COWS. Leroy, J.L.M.R.;Bols, P.E.J.; Opsomer, G.; Van Soom, A.; Kruif, A. .......................................................................................... 5

Short course on new strategies on cooling and freezing equine semen

NEW METHODS ON EQUINE COOLED AND FROZEN SEMEN BIOTECHNOLOGY. Papa, F.O.;Melo, C.M.; Dell’Aqua, J.A.; Macedo, L.P.; Carvalho, A.G.; Alvarenga, M.A.; Medeiros, A.S.L. ............... 19

Short course on embryo transfer in small ruminants

SHEEP AND GOAT EMBRYO TRANSFER. Gusmão, A.L.; Andrade Moura, J.C. .................................... 29

CONFERENCES

TWENTY YEARS HISTORY OF THE BRAZILIAN EMBRYO TECHNOLOGY SOCIETY (1985-2005). Rubin, M.I.B. ....................................................................................................................................... 35

ADVANCES IN THE UNDERSTANDING OF FOLLICLE DEVELOPMENTAL PHYSIOLOGY.Buratini Jr., J.; Castilho, A.C.S.; Teixeira, A.B.; Costa, I.B.; Pinto, M.G.L.; Giometti, I.C.; Glapinski,V.F.; Nogueira, M.F.G.; Barros, C.M.; Price, C.A. ......................................................................................... 55

PERSPECTIVES ON THE USE OF BIOTECHNOLOGY IN EQUINE REPRODUCTION.Squires, E.L. .................................................................................................................................................... 69

EVOLUTION OF TRANSGENIC FARM ANIMALS. Niemann, H.; Kues, W.; Carnwath, J.W. ................. 83

TECHNICAL AND BIOLOGICAL ASPECTS OF ULTRASOUND-GUIDED TRANSVAGINAL OOCYTE RETRIEVAL IN THE COW: AN OVERVIEW. Bols, P.E.J.; Leroy, J.L.M.R.; Viana, J.H.M. . 103

IN VITRO PRODUCTION OF BOVINE EMBRYOS AN OBSTETRIC OVERVIEW OF PROBLEMSAND CHALLENGES. Prestes, N.C. .............................................................................................................119

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WORK SHOPS

Practical aspects on AI and ET in small ruminants

PECULIAR ASPECTS IN SHEEP ARTIFICIAL INSEMINATION. Bicudo, S.D.; Azevedo, H.C.; SilvaMaia, M.S.; Sousa, D.B.; Rodello, L. ........................................................................................................... 127

EARLY IDENTIFICATION OF THE FETAL SEX IN SMALL RUMINANTS BYULTRASONOGRAPHY. Santos, M.H.B.; Moraes, E.P.B.X.; Moura, R.T.D.; Lima, P.F.; Reichenbach, H.-D.; Oliveira, M.A.L. ................................................................................................................................ 131

Practical aspects on AI and ET in Equine

USE OF LOW DOSES OF EQUINE PITUITARY EXTRACT TO INDUCE MULTIPLE OVULATIONSIN MARES. Farinasso, A.; Branquinho, J.A.; Rumpf, R.; Alvarenga, M.A. ................................................ 135

STRATEGIES TO IMPROVE REPRODUCTIVE EFFICIENCY IN SUBFERTILE STALLIONS INEMBRYO TRANSFER PROGRAMS. Neves Neto, J.R. ............................................................................. 139

Practical aspects on AI and ET in Bovine

HAS TAI A PROFITABLE BENEFIT COST? Madureira, E.H.; Baruselli, P.S.; Valim Pimentel, J.R.;Almeida, A.B. ................................................................................................................................................ 141

IMPORTANCE OF SEMEN QUALITY IN FIXED-TIME ARTIFICIAL INSEMINATION ANDEMBRYO TRANSFER PROGRAMS. Arruda, R.P.; Celeghini, E.C.C.; Souza, L.W.O.; Nascimento, J.;Andrade, A.F.C.; Raphael, C.F.; Garcia, A.R. ............................................................................................... 145

NEW ADVANCES IN TREATMENTS OF BOVINE EMBRYO DONORS AND RECIPIENTS.Buruselli, P.S.; Martins, C.M.; Sá Filho, M.F.; Nasser, L.F.; Gimenes, L.U.; Madureira, E.H.; Bó, G.A. ... 151

ROUND TABLE DISCUSSION: Problems related to commercial IVP embryos

COMMERCIAL IN VITRO FERTILIZATION CHALLENGES. Queiroz, L.M.V.; Peixer, M.A.S.;Malard, P.F.; Santana, G.M.; Xavier, M.C.; Sousa, R.R. .............................................................................. 159

PROBLEMS RELATED TO COMERCIAL IN VITRO EMBRYO PRODUCTION. Saliba, W.P.;Alvim, M.T.A. ............................................................................................................................................... 162

STUDENTS COMPETITION

EFFECT OF NITRIC OXIDE ADDICTION IN BOVINE OOCYTE DURING MATURATION INVITRO. Viana, K.S.; Caldas-Bussiere, M.C.; Matta, S.G.C.; Paes de Carvalho, C.S.; Faes, M.R.;Quirino, C.R. ................................................................................................................................................ 1 6 9

INFLUENCES OF INDOLE-3-ACETIC ACID ON THE DEVELOPMENT OF CAPRINE PREANTRALFOLLICLES. Matos, M.H.T.; Martins, F.S.; Santos, R.R.; Luque, M.C.A.; Silva, J.R.V.; Celestino,J.J.H.; Báo, S.N.; Figueiredo, J.R. ................................................................................................................ 170

MATURATION AND VITRIFICATION OF EQUINE OOCYTES IN MEDIUN WITH EQUINEGROWTH HORMONE AND INSULIN-LIKE GROWTH FACTOR-I. Curcio, B.R.; Deschamps, J.C.;Nogueira, C.E.W.; Pereira, G.R.; Macedo Jr, M.C.; Boff, A.L.N1; Rambo, G.; Lucia Jr, T. ........................ 171

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DOES THE CYTOPLAST TRANSFER ALTERS THE EMBRYONIC DEVELOPMENT ANDGESTATIONAL TERM DEVELOPMENT IN BOVINE? Ferreira, C.R.; Meirelles, F.V.; Perecin, F.;Méo, S.C.; Tetzner, T.A.D.; Saraiva, N.Z.; Landim Jr., L.P.; Yamazaki, W.; Vantini, R.; Garcia, J.M. ....... 172

EFFECTS OF FOLLICLE-STIMULATING HORMONE ADMINISTRATION IN OOCYTE DONORHEIFERS FOR BOVINE NUCLEAR TRANSFER USING ADULT EAR SKIN FIBROBLASTS OF ABRAZILIAN CREOLE BREED – PRELIMINARY RESULTS. Iguma, L.T.; Pivato, I.; Câmara, J.U.;Damato, J.; Pereira, D.C.; Corrêa, G.A.; Machado, G.M.; Franco, M.M.; Borges, J.R.J.; Rumpf, R. ......... 173

OOCYTE TRANSFER INTO BUFFALO OVIDUCT OF FEMALES WITH SINGLE AND MULTIPLEOVULATIONS. Carvalho, N.A.T.; Gimenes, L.U.; Sá Filho, M.F.; Vannucci, F.S.; Reichert, R.H.;Carvalho, J.B.P.; Souza, R.J.; D’Angelo, M.; Chiari, J.R.; Baruselli, P.S. ................................................... 174

ORAL PRESENTATION

EFFECTS OF THE MOMENT OF PGF2α IN FIXED TIME EMBRYO TRANSFER PROTOCOL ON

SYNCHRONIZATION AND CONCEPTION RATES IN IVF FRESH EMBRYO RECIPIENTS. SáFilho, O.G.; Vasconcelos, J.L.M.; Santos, R.M.; Oba, E.; Perez, G.C.; Araújo, D.B. .................................. 177

EFFECT OF PROGESTERONE SUPPLEMENTATION ON SUPEROVULATORY RESPONSE INNELORE COWS (Bos indicus ) DURING THE FIRST-FOLLICULAR WAVE. Nasser, L.F.T.; Bó,G.A.; Reis, E.L.; Menegati, J.A.; Marques, M.O.; Gimenes, L.U.; Sá Filho, M.F.; Mapletoft, R.J.;Baruselli, P.S. ................................................................................................................................................. 178

EFFECT OF SUPEROVULATORY TREATMENT OF MARES WITH EQUINE PITUITARYEXTRACT ON OOCYTE TRANSPORT TO THE OVIDUCT (Preliminary Results). Carmo, M.T.;Losinno, L.; Aguilar, J.J.; Araujo, G.H.M.; Alvarenga, M.A......................................................................... 179

TREATMENT OF MANGALARGA MARCHADOR MARES WITH PROGESTERONE (P4) ANDALTRENOGEST AIMING THE UTILIZATION AS EMBRYO RECIPIENTS ON DAY TWO AFTEROVULATION. Caiado, J.R.C.; Fonseca, F.A.; Silva, J.F.S.; Fontes, R.S. ................................................... 180

GENE EXPRESSION AT MATERNAL-ZYGOTIC TRANSITION IN IN VITRO FERTILIZED ANDSOMATIC CELL NUCLEAR TRANSFER BOVINE EMBRYOS. Camargo, L.S.A.; Powell, A.M.; Viana,J.H.M.; Sá, W.F.; Ferreira, A.M.; Ramos, A.A.; Wall, R.J. .......................................................................... 181

IN VITRO EFFICIENCY OF TWO DIFFERENT TECHNICAL APPROACHS TO PRODUCE BOVINECLONED EMBRYOS. Feltrin, C.; Forell, F.; dos Santos, L.C.; Vieira, A.D.; Rodrigues, J.L. ................... 182

INFLUENCE OF NELORE BLOOD ON THE IN VIVO PRODUCTION OF OÓCYTES. Rubin, K.C.P.;Pontes, J.H.F.; Nonato Jr., I.; Ereno Jr., J.C.; Pansard, H.; Seneda, M.M. ................................................... 183

EFFECT OF EXOGEN PROGESTERONE IN OOCYTE DONORS COWS ON FOLLICLEDEVELOPMENT AND IN VITRO EMBRYO PRODUCTION. Pfeifer, L.F.; Schneider, A.; Castilho,E.M.; Luz, E.M.; Ataíde, P.F.; Dionello, N.J.L.; Pivato, I.; Rumpf, R.; Corrêa, M.N. ................................. 184

COMPARISON OF PREGNANCY LOSSES IN EMBRYO RECIPIENTS PREGNANT OF Bos indicusEMBRYOS PRODUCED IN VIVO OR IN VITRO. Borsato, E.A.; Ludwig Jr., H.E.; Silva, D.R.M.;Barreiros, T.R.R.; Seneda, M.M. ................................................................................................................... 185

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INVOLUTION OF THE YOLK SAC IN IVF AND NATURAL STUD BOVINE EMBRYOS (10 TO 70DAYS OF GESTATION). Assis Neto, A.C.; Silva, T.C.B.; Verechia, F.T.V.; Ambrósio, C.E.; Almeida,A.B; Meirelles, F.V.; Garcia, J.M.; Miglino, M.A. ........................................................................................ 186

ABSTRACTS - SESSION A

EFFECT OF DIFFERENT SUPEROVULATION PROTOCOLS ON EMBRYO PRODUCTION INMICE (Mus musculus) FROM SWISS LINEAGE Wohlers, S.; Pinto, I.S.B.; Arashiro, E.K.N.;Viana, J.H.M. ................................................................................................................................................. 189

INCIDENCE OF MULTIPLE OVULATIONS IN THE CAMPOLINA BREED AND ITS INFLUENCEON EMBRYO RECOVERY. Greco, G.M.; Oliveira, G.D.M.; Pinna, A.E.; Pinho, T.G.; Queiroz, F.J.R. .... 190

CORRELATION AMONG THE DIAMETER OF THE CORPUS LUTEUM FORMED FROM SINGLEAND DOUBLE OVULATIONS AND THE PLASMATIC LEVEL OF PROGESTERONE IN JENNIES.Conceição, J.C.; Araujo, G.H.M.; Oliveira, J.V.; Oba, E.; Carvalho, L.R.; Meira, C. .................................. 191

EFFECT OF eFSH® TREATMENT ON LUTEAL FUNCTION IN SUPEROVULATEDTRANSITIONAL MARES. Peres, K.R.; Fernandes, C.B.; Oba, E.; Alvarenga, M.A.;Landim-Alvarenga, F.C. ................................................................................................................................ 192

EFFECT OF DESORELIN AND EQUINE PITUTARY EXTRACT ON INDUCTION OF OVULATIONIN CYCLING MARES. Melo, C.M.; Papa, F.O.; Medeiros, A.S.L.; Dell’Aqua Jr., J.A.; Carmo, M.T.;Araújo, G.H.M.; Alvarenga, M.A .................................................................................................................. 193

HORMONAL LEVELS IN MARES SUPEROVULATED WITH PURIFIED EQUINE FSH – PARTIALRESULTS. Machado, M.S.; Araújo, G.H.M.; Roser, J.F.; Alonso, M.A.; Alvarenga, M.A. ........................ 194

USE OF EQUINE PITUITARY EXTRACT AS AN AGENT TO INDUCTION OF OVULATION INMARES. Medeiros, A.S.L.; Silva Jr., B.L.; Melo, C.M.; Carmo, M.T.; Papa, F.O.; Puoli Filho, J.N.P.;Araújo, G.H.M.; Alvarenga, M.A. ................................................................................................................. 195

EFFECT OF LOW DOSES OF EQUINE PITUITARY EXTRACT AND EQUINE PURIFIED FSH ONOVULATION RATE AND EMBRYO RECOVERY RATE IN MARES. Rocha Filho, A.N.; Lopes, E.P.;Araujo, G.H.M.; Alvarenga, M.A. ................................................................................................................. 196

EFFECT OF OVULATION INDUTION WITH HUMAN CHORIONIC GANADOTROPIN ONPROGESTERONE PLASMATIC LEVELS AT EARLY DIESTRUS IN MARES. Araujo, G.H.M.; Dutra,F.O.; Carmo, M.T.; Oba, E.; Alvarenga, M.A. .............................................................................................. 197

EARLY EMBRYONIC DEATH IN ANOVULATORY RECIPIENTS MARES SUPPLEMENTED WITHLONG ACTING PROGESTERONE. Testa, A.C.; Carmo, M.T.; Alvarenga, M.A. ............................................................... 198

COMPARISION BETWEEN TWO DIFFERENT MEDIA FOR EQUINE EMBRYO TRANSFER INMANGALARGA MARCHADOR MARES. Caiado, J.R.C.; Fonseca, F.A.; Silva, J.F.S.; Fontes, R.S. .... 199

USE OF hCG AIMING AT IMPROVEMENTS OF SOME REPRODUCTIVE CHARACTERISTICSOF EMBRYOS RECIPIENTS MARES. Sousa, F.A.C.; Fleury, P.D.C.; Alonso, M.A.; Arruda, R.P. ........ 200

ENDOMETRIAL BIOPSY SCORE AND REPRODUCTIVE APTITUDE IN EMBRYO RECIPIENTMARES. PRELIMINARY RESULTS. Losinno, L.; Alonso, C.; Castaneira, C. ......................................... 201

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EFFECT OF COOLED SEMEN ON EQUINE EMBRYO RECOVERY RATE. Duarte, M.B.;Vieira, R.C. .................................................................................................................................................... 202

EFFECT OF DONOR AGE ON EQUINE EMBRYO RECOVERY RATE. Duarte, M.B.; Vieira, R.C. .... 203

EFFECT OF DONOR MARE’S AGE IN EMBRYONIC LOSS RATE. Alonso, M.A.; Fleury, P.D.C.;Neves Neto, J.R.; Machado, M.S. .................................................................................................................................................................................................... 204

PREGNANCY RATES AFTER NONSURGICAL TRANSFER OF EQUINE EMBRYOS STORED INEMCARE OR VIGRO HOLDING MEDIA FOR 18H AT 15 TO 20ºC. Fleury, P.D.C.; Neves Neto, J.R.;Alonso, M.A.; Squires, E.L. .......................................................................................................................... 205

FIRST BIRTH OF TWO FOALS FROM HORSE EMBRYO SPLITTING IN LATIN AMERICA. Silveira,L.L.; Pimentel, C.M.; Melo, N.S.S.; Souza, R.V.; Rumpf, R. ...................................................................... 206

GYNECOLOGYCAL STUDY OF FEMALE BOVINES DESTINATED TO ARTIFICIALINSEMINATION PROGRAMS OR AS EMBRYO RECEPTORS: PRELIMINARY RESULTS. DelRei, A.J.; Bartolomeu, C.C.; Neves, A.L.A.; Balbino, S.C.; Ferraz, P.C. ..................................................... 207

AVERAGE NUMBER AND SIZE OF OVARIAN STRUCTURES FROM NULLIPAROUS GIR ANDHOLSTEIN EMBRYO DONNORS. Ferreira, M.B.D.; Lopes, B.C.; Souza, J.C.; Azevedo, N.A.;Ordones, F. ..................................................................................................................................................... 208

OVULATORY CAPACITY IN Bos indicus HEIFERS. Gimenes, L.U.; Carvalho, N.A.T.; Sá Filho,M.F.; Santiago, L.L.; Carvalho, J.B.P.; Mapletoft, R.J.; Barros, C.M.; Baruselli, P.S. ................................ 209

ULTRASONOGRAPHIC STUDY OF FOLLICULAR DEVIATION IN NELORE (Bos indicus)HEIFERS Gimenes, L.U.; Sá Filho, M.F.; Madureira, E.H.; Trinca, L.A.; Barros, C.M.; Baruselli, P.S. ... 210

EFFICIENCY OF ARTIFICIAL INSEMINATION IN FIXED TIME COMBINED WITHPROGESTERONE INTRAVAGINAL DEVICE AND FERTILITY RATES OF FEMALE BUFFALOESRAISED IN DIFFERENT PRODUCTION SYSTEMS. Ribeiro, H.F.L.; Picanço, N.S.; Rolim Filho,S.T.; Pinho, R.O.; Araújo, C.V.; Vale, W.G. ...................................................................................................211

EFFECTS OF MANAGEMENT ABOUT PREGNANCY RATE IN THE ARTIFICIAL INSEMINATIONPROGRAM. Silva, M.R.; Borges, J.C.; Guimarães, J.D. ............................................................................. 212

COMPARISON PREGNANCY RATES ACCORDING TO CORPUS LUTEUM SIZE OFINOVULATED EMBRYO RECIPIENTS WITH Bos indicus EMBRYOS PRODUCED IN VITRO.Borsato, E.A.; Ludwig Jr., H.E.; Silva, D.R.M.; Barreiros, T.R.R.; Seneda, M.M. ..................................... 213

VARIABILITY IN THE NUMBER OF EMBRYOS AT THE OPTIMAL NUMBER OF RECIPIENTDETERMINATION. Beltrame, R.T.; Barioni, L.G.; Quirino, C.R.; Fontes, R.S. ........................................ 214

MEAN COST FOR THE RECIPIENTS TO EMBRYO TRANSFER. Beltrame, R.T.; Barioni, L.G.;Quirino, C.R.; Fontes, R.S. ........................................................................................................................... 215

GESTATION RATE OF HEIFERS FED MEGALAC OR LINSEED GRAIN FOLLOWING TRANSFEROF EMBRYO COLLECTED FROM DAIRY COWS FED MEGALAC OR LINSEED GRAIN. Cavalieri,F.L.B.; Santos, G.T.; Petit, H.; Morgan, J.; Rigolon, L.P.R.; Ramos, F.S.R.; Capovilla, L.C.T.;Albuquerque, K. ............................................................................................................................................. 216

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EFFECTS OF TWO SOURCES OF FAT (MEGALAC® OR LINSEED GRAIN) IN THE DIET IN THEPRODUCTION OF THE EMBRYOS IN DAIRY COW. Cavalieri, F.L.B.; Santos, G.T.; Petit, H.; Morgan,J.; Rigolon, L.P.R.; Ramos, F.S.R.; Capovilla, L.C.T.; Albuquerque, K. ...................................................... 217

DETERMINATION OF ANCESTRAL PROPORTIONS IN SYNTHETIC BOVINE RACES USINGDNA MARKERS: APPLICATION TO INDIVIDUAL GIROLANDO ANIMALS. Bicalho, H.M.S.;Pimenta, C.G.; Mendes, I.K.P.; Pena, H.B.; Lopes, C.; Queiroz, E.M.; Rocha, J.L.F.; Pena, S.D.J. ........... 218

PREGNANCY RATES IN EMBRYO RECIPIENTS TREATED WITH PROGESTERONE RELEASINGDEVICES CONTAINING TWO DIFFERENT PROGESTERONE CONCENTRATIONS. Peres, L. C.;Pincinato, D.; Balla, E.; Tribulo, R.; Bó, G.A. .............................................................................................. 219

PREGNANCY RATES IN RECIPIENT COWS TREATED WITH PROGESTERONE VAGINALDEVICES AND INDUCED TO OVULATE WITH ESTRADIOL BENZOATE GIVEN AT THE TIMEOF DEVICE REMOVAL OR 24 H LATER. Peres, L.C.; Pincinato, D.; Balla, E.; Maraña Peña, D.;Borges, L.F.K.; Cutaia, L.; Bó, G.A. ............................................................................................................. 220

PREGNANCY RATES IN EMBRYO RECIPIENTS TREATED WITH NORGESTOMET EARIMPLANTS AND ESTRADIOL BENZOATE OR DIFFERENT DOSAGES OF ESTRADIOLVALERATE. Peres, L.C.; Pincinato, D.; Maraña Peña, D.; Borges, L.F.K.; Tribulo, R.;Cutaia, L.; Bó, G.A. ....................................................................................................................................... 221

EVALUATION OF SECOND UTERINE FLUSHING ON EMBRYO RECOVERY RATE IN NELORECOWS – PRELIMINARY RESULTS. Barrreiros, T.R.R.; Bezerra, G.A.; Azevedo, J.R.; Seneda, M.M. .. 222

EVALUATION OF CORPUS LUTEUM, UTERINE CONTRACTILITY AND PROGESTERONE ANDESTRADIOL PLASMATIC CONCENTRATIONS IN RECIPIENTS HEIFERS IN THE DAY OFEMBRYO TRANSFER. Leal, L.S.; Oba, E.; Fernandes, C.A.C.; Sá Filho, O.G. ....................................... 223

COMPARISON OF PREGNANCY RATES IN HEIFERS WITH COMPACT OR CAVITARY CORPUSLUTEUM TRANSFERRED WITH IN VITRO PRODUCED EMBRYOS. Borsato, E.A.; Ludwig Jr.,H.E.; Silva, D.R.M.; Barreiros, T.R.R.; Seneda, M.M. ................................................................................ 224

EFFECT OF MOMENT OF PGF2α ADMINISTRATION AND eCG DOSE IN SYNCHRONIZATIONOF OVULATION FOR FIXED-TIME EMBRYO TRANSFER IN HEIFERS. Rezende, C.R.; Nasser,L.F.; Rezende, L.F.; Nunez, M.M.; Vieira, M.P.; Ayres, H.; Sá Filho, M.F.; Gimenes, L.U.; Baruselli, P.S. ..... 225

EFFECT OF LUTEOLISIS TO ESTRUS INTERVAL, PERIOD OF THE YEAR AND FSH DOSE ONEMBRYO PRODUCTION IN BEEF CATTLE. Brauner, C.C.; Bonotto, A.L.M.; Pimentel, M.A.;Pimentel, C.A. ................................................................................................................................................ 226

FOLLICULAR DYNAMICS IN HEIFERS AND COWS (Bos indicus) TREATED WITH NEW ORPREVIOUSLY USED INTRAVAGINAL PROGESTERONE DEVICE ASSOCIATED OR NO TOINJECTION OF PROGESTERONE. Martins, C.M.; Castricini, E.S.C.; Sá Filho, M.F.; Gimenes, L.U.;Baruselli, P.S. ................................................................................................................................................. 227

FIXED-TIME EMBRYO TRANSFER IN Bos taurus x Bos indicus HEIFERS TREATED WITH eCGAT MOMENT INTRAVAGINAL PROGESTERONE RELEASING DEVICE WITHDRAWAL ANDGnRH AT EMBRYO TRANSFER. Nasser, L.F.; Rezende, L.F.; Rezende, C.R.; Vieira, M.P.; Wisnesck,C.A.; Sá Filho, M.F.; Gimenes, L.U.; Baruselli, P.S. .................................................................................... 228

EFFECT OF THE NUMBER OF NORGESTOMET IMPLANT AND OF THE TIME OF OVULATIONINDUCER ADMINISTRATION IN SUPERSTIMULATED HOLSTEIN COWS. Rodrigues, C.;Mancilha, R.F.; Reis, E.L.; Ayres, H.; Gimenes, L.U.; Sá Filho, M.F.; Baruselli, P.S. ................................. 229

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SUPERSTIMULATION WITH FIXED-TIME ARTIFICIAL INSEMINATION IN Bos indicus DONORSTREATED WITH FSHp OR WITH eCG. Chiari, J.R.; Demétrio, D.; Sá Filho, M.F.; Ayres, H.;Baruselli, P.S. ................................................................................................................................................. 230

EFFECT OF SUPEROVULATED REPEATEDLY IN PARDO SUIZO CAWS UNDER TROPICALCONDITIONAL. Solano, R.F.; Dias, J.M.B.; Solano, O.G. ........................................................................ 231

SUPERESTIMULATION IN GENOTIPIC CAWS MAMBI DE CUBA (3/4H x 1/4Z) INDUCED WITHFSH-P AND PMSG IN TROPICAL CLIMATE CONDITION. Solano, R.F.; D’Oliveira-Sousa, A.;Carballo, J.I.; Fuentes, D.S.; Veliz, J.; Solano, O.G. ...................................................................................... 232

ADMINISTRATION OF DIETS WITH SEED OF CANOLA AND SEED OF LINSEED IN THESUPEROVULATORY RESPONSE, PRODUCTION AND QUALITY OF EMBRYOS IN NELORECOWS. Albuquerque, K.P.; Capovilla, L.C.T.; Prado, I.N.; Cavalieri, F.L.B.; Rigolon, L.P.R.;Ramos, F.S.; Tesolin, L.C. ............................................................................................................................. 233

SUPEROVULATORY RESPONSE IN NELORE HEIFERS (Bos indicus) TREATED WITH 250 OR500 UI OF FSHp. Santiago, L.L.; Torres, C.A.A.; Nogueira, E.T.; Amorin, L.; Sá Filho, M.F.; Gimenes,L. U.; Baruselli, P.S. ...................................................................................................................................... 234

OOCYTE TRANSFER INTO BOVINE OVIDUCT OF FEMALES WITH SINGLE AND MULTIPLEOVULATIONS. Carvalho, N.A.T.; Gimenes, L.U.; Sá Filho, M.F.; Vannucci, F.S.; Reichert, R.H.;Carvalho, J.B.P.; Andrade, J.B.; Pires, R.M.L.; Alvarez, R.H.; Meirelles, F.V.; Chiari, J.R.;Baruselli, P.S. ................................................................................................................................................. 235

STATE OF DEVELOPMENT AND PREGNANCY RATE OF DEMI-EMBRYOS. Fernandes, C.A.C.;Oba, E.; Figueiredo, A.C.S.; Ludgero, B.F.A.; Oliveira, E.R.; Gioso, M.M................................................. 236

PREGNANCY CHARACTERISTICS DERIVED FROM BOVINE RECONSTITUTED IN VITROHALF EMBRYOS. Alvarez, R.H.; Amaral, J.B.; Pires, R.M.L.; Martinez, A.C.; Oba, E. .............................................. 237

GESTATION RATE OF INTACT OR SPLIT BOVINE EMBRYOS. Fernandes, C.A.C.; Oba, E.;Figueiredo, A.C.S.; Gioso, M.M.................................................................................................................... 238

EMBRYO BIPARTITION EFFECTIVENESS OF NELORE BREED CONSIDERING PREGANCYRATE. Silveira, E.C.; Constantino-Max, M.; Blaschi, W.; Padilha, L.C.; Francisco, R.C.; Pansard, H.S.;Bortolotti, L.A.; Sgobero Filho, I.; Seneda, M.M. ........................................................................................ 239

BISECTION OF GOAT EMBRYOS – OFFSPRINGS PRELIMINARIES. Rêgo, T.P.; Souza, J.A.; Melo,M.V.L.C.C.; Carmo, D.R.; Sobrinho, J.M.F.; Santos, P. A. C.; .................................................................... 240

FERTILITY OF RECIPIENTS AND EMBRYO SURVIVAL AFTER TRANSFER OF MORADA NOVA(VARIETY WHITE) OVINE EMBRYOS SUBMITTED TO TWO PROCEDURES OF THAWING.Lopes Júnior, E.S.; Maia, E.L.M.M.; Teixeira, D.I.A.; Padilha, R.T.; Almeida, A.P.; Lima, I.M.T.;Albuquerque, C.P.; Pinheiro, E.S.P.; Rondina, D.; Villaroel, A.B.S.; Freitas, V.J.F. ..................................... 241

SERUM CONCENTRATIONS OF PROGESTERONE IN OVARIECTOMIZED EWES TREATEDWITH LONG ACTING PROGESTERONE (P4LA-150). Almeida, A.K.; Martins, L.E.P.; Biscarde,C.E.B.; Neves, T.A.; Chalhoub, M.; Ribeiro Filho, A. de L.; Bringel, B.A.; Douglas, R.H.; Gusmão, A.L. 242

SUPEROVULATORY RESPONSE IN LACTATING SAANEN DOES USING SHORT-TERMPROGESTERONE PROTOCOL AND RECOMBINANT BOVINE SOMATOTROPIN (RBST).Fonseca, J.F.; Viana, J.H.M.; Bruschi, J.H.; Zambrini, F.N.; Palhão, M.P.; Santos, A.F.A. ......................... 243

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PRELIMINARY RESULTS USING A NEW SUPEROVULATORY TREATMENT IN GOATS: THEDAY 0 PROTOCOL. Menchaca, A.; Vilariño, M.; Rubianes, E. .................................................................. 244

EMBRYO TRANSFER IN GOATS: INOVULATION BY LAPAROTOMY. Souza, J.A.T.;Melo, M.V.L.C.C. .......................................................................................................................................... 245

EARLY PREGNANCY DIAGNOSIS BY REAL-TIME ULTRASONOGRAPHY IN SHEEPRECIPIENTS RECEIVING MORADA NOVA (WHITE VARIETY) EMBRYOS. Santos, M.H.B.; LopesJúnior, E.S.; Paula, N.R.O.; Arruda, I.J.; Andrade, M.L.L.; Padilha, R.T.; Freitas, V.J.F.;Oliveira, M.A.L. ............................................................................................................................................ 246

FETAL SEXING BY ULTRASONOGRAPHY IN EWE OF SANTA INÊS BREED. Santos, M.H.B.;Moraes, E.P.B.X.; Rabelo, M.C.; Melo, A.N.; Lima, P.F.; Oliveira, M.A.L. ................................................................................... 247

FETAL SEXING IN GOATS BY ULTRASONOGRAPHY. Santos, M.H.B.; Moraes, E.P.B.X.; Moura,R.T.D.; Chiamenti, A.; Bezerra, F.Q.G.; Aguiar Filho, C.R.; Lima, P.F.; Oliveira, M.A.L. ......................... 248

INDUCTION ESTRUS IN GOATS USING REUSED INTRAVAGINAL DEVICES. Zambrini, F.N.;Fonseca, J.F.; Bruschi, J.H.; Viana, J.H.M.; Palhão, M.P.; Santos, A.F.A. ................................................... 249

PROTOCOLS OF THE SINCHRONIZATION OF ESTROUS AND OVULATION IN RECIPIENTSGOATS. Guido, S.I.; Guido, F.C.L.; Nascimento Filho, E.V.; Oliveira, M.A.L.; Lima, P.F.; Andrade,J.C.O.; Rocha, J.D. ........................................................................................................................................ 250

SYNCHRONIZATION OF ESTRUS IN GOATS FOR SHORT DURATION WITH CIDR-G® ANDINTRAVAGINAL SPONGE. Maffili, V.V.; Torres, C.A.A.; Fonseca, J.F.; Bruschi, J.H.; Viana, J.H.M.;Prosperi, C.P.; Moraes, E.A.; Pontes, R.A.M. .............................................................................................. 251

EFFICIENCY OF THE CIDR® NEW AND REUTILIZED IN PROGRAMS OF ESTRUSSYNCHRONIZATION OF SHORT DURATION IN TOGGENBURG GOATS. Maffili, V.V.; Torres,C.A.A.; Fonseca, J.F.; Bruschi, J.H.; Viana, J.H.M.; Prosperi, C.P.; Moraes, E.A.; Pontes, R.A.M. .......... 252

ESTRUS INDUCTION IN TEXELL EWES DURING THE NON-BREEDING SEASON. Castilho, C.;Mori, M.M.; Alessi, C. .................................................................................................................................. 253

PROGRAM TO SYNCHRONIZE-INDUCE ESTRUS AND OVULATION FOR 12- TO 14-MONTH-OLD HEIFERS. Bragança, J.F.; Loguercio, R.S.; Escobar, F.R.; Loguercio, L.P.; Oliveira, J.F.;Gonçalves, P.B.D. .......................................................................................................................................... 254

VAGINAL DEVICES (PRID®) MICROBIOLOGICAL EVALUATION AFTER UTILIZATION ANDDISINFECTION. Oliveira Filho, B.D.; Gambarini, M.L.; Araújo, I.FL.; Garcia, A.M.; Andrade, M.A. .... 255

EFFECT OF DIFFERENT OVULATORY INDUCTION IN SYNCHRONIZATION RATE. Meneghetti,M.; Losi, T.C.; Martins Jr., A.P.; Vilela, E.R.; Vasconcelos, J.L.M. ............................................................. 256

EFFICIENCY OF DIFERENT DOSES OF FERTIRELIN ACETATE ON FOLLICULAR DINAMICSAND PROGESTERONE CONCENTRATIONS IN DAIRY COWS SUBMITTED TO THE OVSYNCHPROTOCOL. Gioso, M.M.; Fernandes, C.A.C.; Oba, E.; Carvalho, M.A.P.; Oliveira, E.R. ...................... 257

SYNCHRONIZATION OF OVULATION IN ANESTROUS NELORE COWS, TREATED WITHHORMONAL PROTOCOL WITHOUT PROGESTERONE OR PROGESTAGENS. Souza, A.F.;Pinheiro, V.G.; Ereno, R.L.; Barros, C.M. ..................................................................................................... 258

XIV


SYNCHRONIZATION OF BOVINE RECIPIENT’S ESTRUS WITH GnRH AND Hcg. Machado, V.C.;Dias, A.J.B. .................................................................................................................................................... 259

VARIATION ON CONCEPTION RATES IN SUCKLING NELORE COWS SYNCHRONIZED WITHINTRAVAGINAL PROGESTERONE RELEASING DEVICE ASSOCIATED WITH ESTRADIOLBENZOATE OR CYPIONATE. Penteado, L.; Sá Filho, M.F.; Martins, C.M.; Gimenes, L.U.; Ayres, H.;Baruselli, P.S. ................................................................................................................................................. 260

FOLLICULAR DYNAMICS OF PREPUBERTAL NELORE (Bos indicus) HEIFERS TREATED WITHAURICULAR NORGESTOMET IMPLANT AND ESTRADIOL BENZOATE ASSOCIATED OR NOTTO PROGESTERONE INJECTION. Sá Filho, M.F.; Gimenes, L.U.; Madureira, E.H.; Baruselli, P.S. .... 261

EFFECT OF PGF2α ADMINISTRATION IN THE BEGINNING OF AURICULAR NORGESTOMETIMPLANT TREATMENT ON FOLLICULAR DYNAMICS OF NELORE HEIFERS (Bos indicus).Torres-Júnior, J.R.S.; Sá Filho, M.F.; Gimenes, L.U.; Madureira, E.H.; Baruselli, P.S. .............................. 262

TREATMENT WITH hCG PLUS LH, USED TO INDUCE OVULATION IN SUPERSTIMULATEDCOWS, DOES NOT ALTER EMBRYO PRODUCTION NOR PREGNANCY RATES. Nogueira, M.F.G.;Buratini Jr., J.; Barros, C.M. ......................................................................................................................... 263

EFFECT OF LENGTH OF INTRAVAGINAL PROGESTERONE DEVICE EXPOSURE ONPREGNANCY RATES IN BEEF HEIFERS. Gattass, C.B.A.; Sá Filho, O.G.; Vasconcelos, J.L.M. ......... 264

CYCLICITY AND eCG TREATMENT EFFECTS ON FOLLICULAR DYNAMICS ANDCONCEPTION RATE IN NELORE HEIFERS TREATED WITH NORGESTOMET AURICULARIMPLANT AND ESTRADIOL BENZOATE. Sá Filho, M.F.; Penteado, L.; Reis, E.L.; Gimenes, L.U.;Baruselli, P.S. ................................................................................................................................................. 265

FOLLICULAR DYNAMICS IN Bos indicus HEIFERS TREATED WITH NORGESTOMETAURICULAR IMPLANT OR PROGESTERONE INTRAVAGINAL RELEASING DEVICE. Sá Filho,M.F.; Gimenes, L.U.; Ayres, H.; Carvalho, J.B.; Carvalho, N.A.T.; Baruselli, P.S. ..................................... 266

EFFECT OF DIFFERENT LEVELS OF CONCENTRATE SUPPLEMENTATION DURING POS-PARTUM AND THE EFFECTS IN OVARIAN DYNAMICS OF DAIRY COWS. Rigo, A.G.; Salmazo,R.; Schroeder, R.V.; Mizubuti, I.Y.; Costa, M.C.; Seneda, M.M. ................................................................. 267

SYNCHRONIZATION OF OVULATION FOR FIXED TIMED ARTIFICIAL INSEMINATIONDURING THE REPRODUCTIVE SEASON IN CROSSBRED EWES. Castilho, C.; Mori, M.M.;Tomé, R.S. ..................................................................................................................................................... 268

INDUCTION OF OVULATION WITH GnRH OR ESTRADIOL BENZOATE AND TWO-DAY ORFIXED-TIME ARTIFICIAL INSEMINATION IN ESTRUS INDUCTION PROTOCOLS FORPOSTPARTUM BEEF COWS. Siqueira, L.C.; Oliveira, J.F.C.; Loguercio, R.S.; Kurtz, H.;Gonçalves, P.B.D. .......................................................................................................................................... 269

SYNCHRONIZATION OF OVULATION IN PRE-PUBERTAL NELORE AND BONSMARA HEIFERS,TREATED WITH PROGESTERONE RELEASE INTRAVAGINAL DEVICE (PROTOCOL PEPE).Ereno, R.L.; Pegorer, M.F.; Nogueira, M.F.G.; Barros, C.M. ....................................................................... 270

EFFICIENCY OF D-CLOPROSTENOL FOR ESTRUS SYNCHRONIZATION AT DIFFERENT DAYSOF ESTRUS CYCLE. Cembranelli, M.A.R.; Fernandes, C.A.C.; Gioso, M.M.; Figueiredo, A.C.S.;Oliveira, E.R.; Viana, J.H.M. ........................................................................................................................ 271

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STUDY OF SOME ASPECTS THAT CAN INTERFERE ON THE ZEBU FEMALES PREGNANCYIN SYNCHRONIZED OVULATION PROGRAM FOR FIXED TIME INSEMINATION. Bittencourt,R.F.; Ribeiro Filho, A. de L.; Chalhoub, M.; Alves, S.G.G.; Biscarde, C.E.A.; Almeida, A.K.; Portela,A.P.M.; Freitas, D.S.; Silva, A.A.B.; Santana, R.C.M. ................................................................................ 272

ESTRUS BEHAVIOR AND OVULATION RATE IN NELORE FEMALES SYNCHRONIZED WITHPROGESTIN, ESTRADIOL AND EQUINE CORIONIC GONADOTROPHIN. Bergamaschi, M.A.C.M.;Vicente, W.R.R.; Barbosa, R.T.; Machado, R.; Ibiapina, B.T.; Baruselli, P.S.; Alencar, M.M.; Binelli, M. ....... 273

REUTILIZATION OF NORGESTOMET IMPLANTS IN NELORE COWS. Almeida, A.B.; Madureira,E.H.; Binelli, M.; Rossa, L.A.; Pimentel, J.R.V.; Bertan, C.; Marques, V.M. ............................................... 274

EFFECT OF THE REUSAGE OF INTRAVAGINAL DEVICE CRONIPRES® SUPLEMENTED WITHRINGS OF PROGESTERONE IN NELORE COWS INSEMINATED AT FIXED TIME. Duarte, R.A.;Kozicki, L.E.; Bó, G.A.; Gonçalves, R.; Rosa, S.D. ..................................................................................... 275

EFFECT OF TREATMENT WITH 0.5 MG ESTRADIOL BENZOATE GIVEN AT THE TIME OFCIDR-B INSERTION IN AN ESTRUS RESINCRONIZATION PROGRAM IN HEIFERS. Balla, E.;Maraña Peña, D.; Chesta, P.; Pincinato, D.; Peres, L.C.; Tríbulo, R.; Bó, G.A. ........................................... 276

EFFECT OF DOSAGE OF ESTRADIOL BENZOATE ON PREGNANCY RATES IN HEIFERSSYNCHRONIZED WITH CIDR-B DEVICES. Balla, E.; Maraña Peña, D.; Chesta, P.; Pincinato, D.;Peres, L.C.; Tríbulo, R.; Bó, G.A. ................................................................................................................. 277

PREGNANCY RATES IN NELORE COWS AFTER TIMED ARTIFICIAL INSEMINATION ANDSUPPLEMENTATION WITH LUTEOTROPHIC OR ANTI-LUTEOLYTIC AGENTS. Machado, R.;Bergamaschi, M.A.C.M.; Barbosa, R.T.; Alencar, M.M.; Madureira, E.H.; Barbosa, P.F.; Bisinotto, R.S.;Binelli, M. ...................................................................................................................................................... 278

OVULATION RATE IN FEMALE BUFFALOES INSEMINATED WITH A FIXED TIME PROTOCOL.Stella, E.D.; Oba, E.; Mota, A.V.; Lima Neto, J.F.; Gioso, M.M. ................................................................. 279

DO TEMPORARY CALF REMOVAL (TCR) AND/OR ECG ADMINISTRATION INCREASEPREGNANCY RATES IN LACTATING NELORE COWS TREATED WITH PROGESTERONERELEASE INTRAVAGINAL DEVICE? Pinheiro, V.G.; Souza, A.F.; Pegorer, M.F.; Ereno, R.L.;Barros, C.M. .................................................................................................................................................. 280

EFFECT OF THE POST PARTUM INTERVAL ON CONCEPTION RATES IN FIXED TIMEARTIFICIAL INSEMINATION USING CIDR IN NELORE SUCKLED COWS. Barrreiros, T.R.R.;Baruselli, P.S.; Balarin, O.F.; Seneda, M.M. ................................................................................................. 281

ARTIFICIAL INSEMINATION AT FIXED TIME IN HIGH-PRODUCING HOLSTEIN COWS WITHESTRADIOL BENZOATE AND CLOPROSTENOL. Silveira, E.C.; Barrreiros, T.R.R.; Delboni, M.I.;Oliveira, A.C.; Padilha, L.C.; Seneda, M.M. ................................................................................................ 282

EFFICIENCY OF REDUCED DOSES OF FERTIRELIN ACETATE IN OVSYNCH PROGRAMS INDAIRY COWS. Gioso, M.M.; Fernandes, C.A.C.; Oba, E.; Carvalho, M.A.P.; Vasconcelos, T.D. ........... 283

COMPARITIVE STUDY OF TWO PROTOCOLS FOR SYNCHRONIZATION OF OVULATION ANDFIXED-TIME INSEMINATION IN COWS. Abreu, C.O.; Martinez, A.C.; Pires, R.M.L.; Centenaro,E.A.; Bertonsello, L.G.; Zampiva, W.K. ........................................................................................................ 284

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FOLLICULAR DYNAMICS OF NELORE COWS TREATED WITH ESTRADIOL CYPIONATE ORBENZOATE IN FIXED TIME ARTIFICIAL INSEMINATION PROTOCOLS. Martins, C.M.; Castricini,E.S.C.; Sá Filho, M.F.; Gimenes, L.U.; Baruselli, P.S. ................................................................................. 285

EMBRYO PRODUCTION IN HOLSTEIN COWS AT DIFFERENT PROTOCOLS OFSUPERSTIMULATION WITH FIXED TIME ARTIFICIAL INSEMINATION. Martins, C.M.; Castricini,E.S.C.; Reis, E.L.; Torres-Júnior, J.R.S.; Gimenes, L.U.; Sá Filho, M.F.; Baruselli, P.S. ............................ 286

EFFECT OF PGF2α AT INSERTION AND OF eCG AT REMOVAL OF INTRAVAGINALPROGESTERONE RELEASING IN PREGNANCY RATES TO FIXED-TIME ARTIFICIALINSEMINATION IN NELORE HEIFERS. Marques, M.O.; Sá Filho, M.F.; Gimenes, L.U.; Figueiredo,T.B.; Sória, G.F.; Baruselli, P.S. .................................................................................................................... 287

SUPERSTIMULATION WITH FIXED-TIME ARTIFICIAL INSEMINATION IN Bos indicus DONORSTREATED WITH NORGESTOMET AURICULAR IMPLANT OR PROGESTERONEINTRAVAGINAL RELEASING DEVICE. Chiari, J.R.; Demétrio, D.; Sá Filho, M.F.; Ayres, H.;Baruselli, P.S. ................................................................................................................................................. 288

THE INFLUENCE OF HORMONAL DOSES ON THE CONCEPTION RATE IN ZEBU COWSSUBMITTED TO PROTOCOLS OF SYNCHRONIZED OVULATION FOR FIXED TIMEINSEMINATION. Ribeiro Filho, A. de L.; Bittencourt, R.F.; Chalhoub, M.; Alves, S.G.G.; Biscarde,C.E.A.; Almeida, A.K.; Portela, A.P.M.; Freitas, D.S.; Silva, A.A.B.; Santana, R.C.M. ............................. 289

THE INFLUENCE OF CORPORAL SCORE ON THE ZEBU HEIFERS, SUBMITTED TO APROTOCOL OF SYNCHRONIZED OVULATION FOR FIXED TIME INSEMINATION. Bittencourt,R.F.; Ribeiro Filho, A. de L.; Chalhoub, M.; Alves, S.G.G.; Biscarde, C.E.A.; Almeida, A.K.; Portela,A.P.M.; Freitas, D.S.; Silva, A.A.B.; Santana, R.C.M. ................................................................................ 290

USE OF ULTRASOUND TO EVALUATE COWS IN FIXED TIME ARTIFICIAL INSEMINATION(FTAI). Nogueira, E.; Silva, A.S.; Araújo, J.M.; Tolentino, R.L.L. .............................................................. 291

LASER ARTIFICIAL VISION SYSTEM: A TOOL FOR EVALUATION OF SEMEN UTILIZED INREPRODUCTIVE BIOTECHNIQUES. Carvalho, P.H.A.; Barreto Filho, J.B.; Rabelo, G.F.; Braga Jr.,R.A.; Albuquerque, F.T.; Rossi, R.O.S.D. ..................................................................................................... 292

STANDARDIZATION OF A NEW HIPOSMOTIC TEST (HOST) FOR DOG SPERM EVALUATION.Silva, K.V.G.C.; Cunha, I.C.N.; Rocha, A.A.; Lopes, B.V.; Silva, J.F.S. ..................................................... 293

ANLYSIS AND CARACTERIZATION OF THE PROTEINS PROFILE OF SEMINAL PLASMA ANDSPERM CELLS OF STALLION. Araújo, E.B.; Souza, G.V.; Silva, J.F.S.; Fagundes, B. ........................... 294

EFFECT OF TEMPERATURE ON MOTILITY DURING SEMEN COLLECTION AND COOLINGPERIOD OF EQUINE SEMEN. Andrade, M.P.; Silva, J.F.S.; Souza, G.V.; Fagundes, B.; Costa, D.S.;Detmann, E. ................................................................................................................................................... 295

SEMINAL CHARACTERISTICS AND COOLING RESISTANCE OF CRIOULO BREED STALLIONSPERMATOZOA – A PRELIMINARY STUDY. Boff, A.L.N.; Curcio, B.R.; Frey, F.J.; Nogueira, C.E.W.;Torres, A.; Moraes, C.M.; Bongalhardo, D. .................................................................................................. 296

EFFECT OF ANTIBIOTICS ON VIABILITY AND FERTILITY OF EQUINE SEMEN COOLED TO5°C. Macedo, L.P.; Papa, F.O.; Gomes, G.M.; Melo, C.M.; Oliveira, J.V.; Dell’Aqua Jr., J.A. ................... 297

XVII


EVALUATION OF PLASMATIC AND ACROSOMAL MEMBRANES INTEGRITY ANDMITOCHONDRIAL MEMBRANE POTENTIAL ON DIFFERENT CONCENTRATIONS ANDVOLUMES OF EQUINE CRYOPRESERVED SPERMATOZOA. Nascimento, J.; Celeghini, E.C.C.;Raphael, C.F.; Andrade, A.F.C.; Arruda, R.P. ............................................................................................... 298

EFFECTS OF SPERMATIC CONCENTATION AND STRAW VOLUME IN MOTIONCARACTERISTICS OF EQUINE CRYOPRESERVED SPERMATOZOA EVALUATED BYCOMPUTER SYSTEM – CASA. Nascimento, J.; Celeghini, E.C.C.; Andrade, A.F.C.; Raphael, C.F.;Arruda, R.P. ................................................................................................................................................... 299

FERTILITY RATES OF EQUINE SEMEN COOLED FOR 24H BEFORE FREEZING. Melo, C.M.;Zahn, F.S.; Martin, I.; Trinque, C.L.A.; Alberti, K.; Orlandi, C.; Siqueira-Filho, E.R.; Dell’Aqua Jr., J.A.;Alvarenga, M.A.; Papa, F.O. ......................................................................................................................... 300

EFFECT OF DIFFERENT CONCENTRATIONS OF CRYOPROTECTORS ON EQUINE SEMENFREEZING. Melo, N.S.S.; Pimentel, C.M.; Silveira, L.L.; Mariante, A.S. .................................................. 301

THE INFLUENCE OF DIFFERENT CRYOPROTECTANTS AND ITS ASSOCIATIONS ON EQUINEFROZEN SEMEN. Dores, C.B.; Papa, F.O.; Blanes, M.; Melo, C.M.; Dell’Aqua Jr., J.A.; Alberti, K.;Crocci, A.J. .................................................................................................................................................... 302

EFFECT OF SPERM CONCENTRATION AND STABILIZATION TIME ON EQUINE FROZENSEMEN USING BOTU-CRIO® EXTENDER Blanes, M.S.; Papa, F.O.; Dores, C.B.; Melo, C.M.;Crocci, A.J. .................................................................................................................................................... 303

EFFICIENCY OF EQUINE CRYOPRESERVED SEMEN APPLICATION IN ARTIFICIALINSEMINATION AND EMBRYO TRANSFER PROGRAM. Granito, A.L.; Nascimento, J.;Arruda, R.P. ................................................................................................................................................... 304

EFFECT OF BODY WEIGHT OVER SEXUAL DEVELOPMENT PARAMETERS IN SANTA INEZLAMB RAMS Siqueira Filho, E.R.; Alberti, K.; Bicudo, S.D. .................................................................... 305

PROTEINS AND CHOLESTEROL OF RAM SEMINAL PLASMA WITH DIFFERENT LEVELS OFFREEZABILITY. Câmara, D.R.; Simplício, K.M.M.G.; Porto, A.L.F.; Padilha, R.T.; Cavalcanti, M.T.H.;Moreira, K.A.; Guerra, M.M.P. ..................................................................................................................... 306

INTRAUTERINE ARTIFICIAL INSEMINATION IN SHEEP THROUGH THE CERVICAL VIA. Silva,J.C.B.; Oliveira, R.; Schneider, C.; Traldi, A.S. ............................................................................................ 307

USE OF SEMINAL PLASMA TO THAW RAM SEMEN FOR TRANS-CERVICAL ARTIFICIALINSEMINATION (AI) IN EWE WITH SYNCHRONIZED ESTRUS. Silva, T.A.S.N.; Neves, J.P.;Bragança, J.M.; Gonçalves, P.B.D.; Rumpf, R.. ........................................................................................... 308

ARTIFICIAL INSEMINATION OF GOAT USING SEMEN EXTENDER AND CRYOPRESERVEDWITH TES. Silva, A.O.A.; Sousa, J.S.; Reis, A.N.; Oliveira, C.M.C.; Silva, L.K.X.; Vale, W.G.;Ribeiro, H.F.L. ............................................................................................................................................... 309

CRYOPRESERVATION OF RAM SEMEN IN TREHALOSE SOLUTION. Valleriote, P.S.; Dias, A.J.B.;Paes de Carvalho, F.; Paes Sobrinho, C. ........................................................................................................ 310

EFFECT OF EQUEX-STM ADDICTION IN EGG YOLK-TRIS EXTENDER ON THE MOTILITYOF FROZEN-THAWED RAM SPERMATOZOA. Maia, M.S.; Azevedo, H.C.; Bicudo, S.D.; Sousa,D.B.; Rodello, L. ............................................................................................................................................311

XVIII


INFLUENCE OF THE TEMPERATURE IN THE TESTICULAR DEGENERATIVE PROCESS OFBULLS IN REGIMEN OF SEMEN COLLECTION. Silva, L.K.X.; Silva, A.O.A.; Reis, A.N.; Sousa,J.S.; Ribeiro, H.F.L. ....................................................................................................................................... 312

TESTICULAR BIOMETRY AND HISTOPATHOLOGY OF NELORE BULLS. Goiozo, P.F.I.; Di Santis,G.W.; De Moura, V.M.B.D.; Bandarra, E.P. .................................................................................................. 313

EVALUATION OF TESTOSTERONE PLASMA LEVELS AND DIFFERENCES AMONG RECTAL,ESCROTAL AND INTRATESTICULAR TEMPERATURES AND NUMBER OF SWEAT ANDSEBACEOUS GLANDS IN BUFFALOS’ SCROTUM (Bubalus bubalis) OF TWO AGE GROUPS.Satrapa, R.A.; Diniz, E.G.; Beletti, M.E.; Eberhardt, B.G.; Satrapa, R. ....................................................... 314

IGFBP-3 LEVEL ASSESSMENT IN BULL SEMINAL PLASMA: TECHNIQUE VALIDATION. Viau,P.; Oliveira, C.A.; Souza, L.W.O.; Arruda, R.P. ........................................................................................... 315

IMMUNOASSAY OF INSULIN-LIKE GROWTH FACTOR-I IN BULL SEMINAL PLASMA. Oliveira,C.A.; Viau, P.; Felippe, E.C.G.; Souza, L.W.O.; Arruda, R.P.; Negrão, J.A. ................................................ 316

NON-EXTRACTIVE IMMUNOASSAY FOR BOVINE SEMINAL PLASMA TESTOSTERONE.Oliveira, C.A.; Viau, P.; Felippe, E.C.G.; Souza, L.W.O.; Arruda, R.P.; Negrão, J.A. ................................. 317

CHARACTERIZATION OF BINDING-HEPARIN PROTEINS IN THE SEMINAL PLASMA ANDSPERMATOZOIDS IN NELORE BULLS. Fernandes, C. E.; Souza, F.F.; Neto, J.S.; Ribolla, P.E.;Feliciano Silva, A.E.D. .................................................................................................................................. 318

EFFECTS OF SOMATOTROPIN ON THE SEMINAL CHARACTERISTICS OF Bos taurus taurusUNDER TESTICULAR HEAT STRESS CONDITIONS. Souza, L.W.O.; Garcia, A.R.; Celeghini, E.C.C.;Andrade, A.C.F.; Raphael, C.F.; Nascimento, J.; Rodrigues, P.H.M.; Arruda, R.P. ..................................... 319

EFFECTS OF BOVINE RECOMBINANT SOMATOTROPIN ON CHROMATIN STRUCTURE OFBULL SPERMATOZOA. Garcia, A.R.; Souza, L.W.O.; Celeghini, E.C.C.; Rodrigues, P.H.M.; Andrade,A.F.C.; Raphael, C.F.; Nascimento, J.; Arruda, R.P. ..................................................................................... 320

USE OF CMXROS AND JC-1 ON MITOCHONDRIAL FUNCTION EVALUATION, ASSOCIATEDTO FLUORESCENT PROBES TO PLASMATIC AND ACROSOMAL MEMBRANES EVALUATIONIN BOVINE SPERMATOZOA. Celeghini, E.C.C.; Nascimento, J.; Andrade, A.F.C.; Raphael, C.F.;Souza, L.W.O.; Arruda, R.P. ......................................................................................................................... 321

ULTRAESTRUCTURAL AND DNA FRAGMENTATION ASSESSMENT OF BOVINESPERMATOZOA CONSERVATED BY DIFFERENT TREATMENTS OF SPERM DRIED. Martins,C.F.; Báo, S.N.; Rumpf, R. ........................................................................................................................... 322

EFFECT OF PERCOLL GRADIENT ON THE ACROSOMAL INTEGRITY AND SPERMCHROMATIN FRAGMENTATION IN BULLS WITH ABNORMAL SPERMIOGENESIS. Fernandes,C.E.; Feliciano Silva, A.E.D.; Dode, M.A.N. ................................................................................................ 323

EFFECTS OF SEMINAL PLASMA REMOVAL ON BOVINE FROZEN SEMEN. Alberti, K.; Papa,F.O.; Siqueira Filho, E.R.; Melo, C.M.; Dell’Aqua Jr., J.A. ......................................................................... 324

BOTU-BOV® A NEW PROPOSAL FOR AN EXTENDER IN CRYOPRESERVATION OF BOVINESEMEN. Alberti, K.; Papa, F.O.; Siqueira Filho, E.R.; Melo, C.M.; Dell’Aqua Jr., J.A. ............................ 325

XIX


EFFECT OF BOTU-BOV® AND TRIS EXTENDERS ON SEMEN FREEZABILITY IN BULLS Bostaurus taurus & Bos taurus indicus Alberti, K.; Papa, F.O.; Siqueira Filho, E.R.; Melo, C.M.;Dell’Aqua Jr., J.A. ......................................................................................................................................... 326

EFFECTS OF CRYOPRESERVATION AND DILUENT ON BOVINE SEMEN WITH REGARD TOPLASMATIC AND ACROSOMAL MEMBRANES AND MITOCHONDRIAL FUNCTION Celeghini,E.C.C.; Andrade, A.F.C.; Nascimento, J.; Raphael, C.F.; Bianconi, L.L.; Rodrigues, P.H.M.; Arruda, R.P. ...... 327

EFFECTS OF CRYOPRESERVATION AND DILUENT ON MOTILITY OF BOVINE SEMENCeleghini, E.C.C.; Andrade, A.F.C.; Nascimento, J.; Raphael, C.F.; Rodrigues, P.H.M.; Arruda, R.P. ....... 328

EFFECTS OF CRYOPRESERVATION USING DIFFERENT FREEZING TECHNIQUES ANDCRYOPROTECTANTS ON PLASMATIC, ACROSOMAL AND MITOCHONDRIAL MEMBRANESOF BOVINE SPERMATOZOA. Arruda, R.P.; Forero-Gonzalez, R.A.; Celeghini, E.C.C.;Raphael, C.F. .....................................................................................................................................................329

ABSTRACTS – SESSION B

IGF-I CONCENTRATIONS IN FOLLICLES FROM NELORE COWS. Melo, D.S.; Leal, L.S.; Gioso,M.M.; Costa, I.B.; Machado, M.S.; Assaf, S.S.; Fernandes, C.B.; Oba, E. ................................................. 333

FIBROBLASTIC GROWTH FACTOR 10 (FGF-10) GENE EXPRESSION DURING BOVINE LUTEALDEVELOPMENT. Giometti, I.C.; Castilho, A.C.S.; Andrade, P.B.; Costa, I. B.; Machado, M.F.; Price,C.A.; Papa, P.C.; Buratini Jr., J. .................................................................................................................... 334

REGULATION OF FIBROBLAST GROWTH FACTOR RECEPTOR-2b (FGFR-2b) GENEEXPRESSION IN CULTURED BOVINE GRANULOSA CELLS. Buratini Jr., J.; Castilho, A.C.S.;Cao, M.; Nicola, E.S.; Price, C.A. ................................................................................................................ 335

IDENTIFICATION OF CONEXIN-37 AND CONEXIN-43 IN PREANTRALS FOLLICLES OFOVARIES OF BOVINE FETUSES OF THE NELORE BREED. Basso, A.C.; Tetzner, T.A.D.;Garcia, J.M.; Esper, C.R. .............................................................................................................................. 336

EXPRESSION OF THE FULL-LENGTH AND ALTERNATIVELY SPLICED BOVINE LH RECEPTORmRNAs IN THECA AND GRANULOSA CELLS FROM ANTRAL FOLLICLES. Nogueira, M.F.G.;Pinto, M.L.G.; Rainho, C.A.; Avellar, M.C.W.; Price, C.A.; Buratini Jr., J.; Barros, C.M. ......................... 337

DETECTION OF ENDOTHELIAL NITRIC OXIDE SYNTHASE ENZYME (eNOS) IN BOVINEOVARIES. Natori, M.M.; Santos, N.P.; Adona, P.R.; Pires, P.R.L.; Caldas-Bussiere, M.C.;Leal, C.L.V. ................................................................................................................................................... 338

NITRIC OXIDE EFFECT ON GRANULOSA CELLS APOPTOSIS IN ZEBU COWS. Faes, M.R.;Moreira, L.; Carvalho, C.S.P.; Caldas-Bussiere, M.C. ................................................................................. 339

QUANTITATIVE AND MORPHOMETRIC ANALYSIS OF PREANTRAL FOLLICLES OFBUFFALOES FETUSES: PRELIMINARY RESULTS. Santos, S.D.S.; Carvalho, A.C.; Ferreira, M.A.P.;Silva, M.S.; Silva, E.S.; Cordeiro, M.S.; Biondi, F.C.; Ohashi, O.M. .......................................................... 340

CRYOPRESERVATION OF BOVINE PREANTRAL FOLLICLES. Celestino, J.J.H.; Santos, R.R.;Matos, M.H.T.; Martins, F.S.; Costa, S.H.F.; Silva, J.R.V.; Rodrigues, A.P.R.; Figueiredo, J.R. ................ 341

XX


VIABILITY OF PRIMORDIAL FOLLICLES FROM CRYOPRESERVED OVARIAN CORTICALTISSUE IN SHEEP. Tsuribe, P. M.; Landim-Alvarenga, F.C. ...................................................................... 342

ULTRA STRUCTURAL CHARACTERIZATION OF BUFFALO (Bubalus bubalis) PREANTRALFOLLICLES. Mondadori, R.G.; Luque, M.C.A.; Santin, T.R.; Báo, S.N. .................................................. 343

ULTRASTRUCTURAL ANALYSIS OF OVINE PREANTRAL FOLLICLES CULTURED IN THEPRESENCE OF INDOL ACETIC ACID, EGF AND FSH. Andrade, E.R.; Landim-Alvarenga, F.C.;Blaschi, W.; Alfieri, A.A.; Seneda, M.M.; Figueiredo, J.R.; Toniolli, R. ...................................................... 344

THE EFFECT OF DIFFERENT CONCENTRATIONS OF 3 INDOL ACETIC ACID IN THE BOVINEPREANTRAL FOLLICLES CULTURE: PRELIMARY RESULTS. Blaschi, W.; Andrade, E.R.; Delboni,M.I.; Boselli, C.C.; Bracarense, A.P.F.R.L.; Alfieri, A.A.; Figueiredo, J.R.; Seneda, M.M. ........................ 345

EMBRYO CULTURE CONTAMINATION USING COMMERCIAL SEMEN SAMPLES: CASEREPORT. D’Angelo, M.; Vieira, S.E.M; Pavão, D.L.; Malaguti, J.M.A.; Rojas, N.; Souza, R.J.; Athayde,C.; Ruyz, D.C.; Galuppo, A.G.; Pinheiro, E.S. .............................................................................................. 346

FIRST RESULTS WITH IVF USING SEXED SPERMATOZOA IN BRAZIL. Oliveira, K.M.; Franco,R.V.R.; Diniz, E.G.; Vieira, F.V.; Galassi, A.G.; Tavares, M.; Braga, M.Q.; Galeli, G.; Rodrigues, L.F. .... 347

IN VITRO PRODUCTION (IVP) OF BOVINE EMBRYOS USING SEXED SPERMATOZOA. Brum,D.S.; Leivas, F.G.; Saliba, W.P.; Alvim, M.T.T. ............................................................................................ 348

IN VITRO PRODUCTION OF BOVINE (Bos taurus indicus) EMBRYOS USING SEXEDSPERMATOZOA. Ereno, A.F.C.; Andrade, R.F.; Pontes, J.H.F. ................................................................. 349

IN VITRO PRODUCTION OF BOVINE EMBRYOS USING SEXED SPERM. Tonello, T.T.M.; Accorsi,M.F.; Ferraz, M.L.; Watanabe, M.R.; Meirelles, F.D.P.; Meirelles, F.V.; Watanabe, Y.F. ............................ 350

EFFECT OF SPERMATOZOA ON THE IN VITRO MATURATION FROM BITCHES (Canis familliaris)OOCYTES. Rocha, A.A.; Bastos, R.; Cunha, I.C.N.; Quirino, C.R.; de Souza, D.B.; Paes deCarvalho, C.S. ............................................................................................................................................... 351

SPERM PENETRATION IN PIG OOCYTES SUBMITTED TO DIFFERENT MATURATIONSYSTEMS. Lima, A.S.; Nascimento, A.B.; Oliveira, V.P.; Marques, M.G.; Assumpção, M.E.O.A.;Visintin, J. A. ................................................................................................................................................. 352

INFLUENCE OF DIFFERENT SPERM CONCENTRATIONS AND PROTEIN SOURCES ONCLEAVAGE AND IN VITRO EMBRYOS DEVELOPMENT RATES IN PIGS. Nascimento, A.B.;Marques, M.G.; Oliveira, V.P.; Coutinho, A.R.S.; Gonçalves, J.S.A.; Assumpção, M.E.O.A.;Visintin, J.A. .................................................................................................................................................. 353

EFFECT OF BOARS, EJACULATES, EXTENDERS AND CAFFEINE ON THE RATES OF IN VITROSPERM CAPACITATION. Nascimento, A.B.; Nichi, M.; Coutinho, A.R.S.; Marques, M.G.; Oliveira,V.P.; Gonçalves, J.S.A.; Assumpção, M.E.O.A.; Visintin, J.A. ..................................................................... 354

EFFECTS OF ANTIOXIDANTS DURING IN VITRO FERTILIZATION ON QUALITY ANDFERTILITY OF BOVINE SPERMATOZOA. Gonçalves, F.S.; Barretto, L.S.S.; Arruda, R.P.;Mingoti, G.Z. ................................................................................................................................................. 355

XXI


EFFECT OF IN VITRO FERTILIZATION PERIOD AND CAFFEINE ON REDUCTION OFPOLYSPERMIC EMBRYOS IN PIGS Oliveira, V.P.; Marques, M.G.; Nascimento, A.B.; Gonçalves,J.S.; Mendes, C.M.; Assumpção, M.E.O.A.; Visintin, J.A. ........................................................................... 356

MITOCHONDRIAL ACTIVITY IN BOVINE SPERMATOZOA INCUBATED AT DIFFERENTPERIODS WITH OR WITHOUT EXOGENOUS DNA. Feitosa, W.B.; Rovegno, M.; Simões, R.;Milazzotto, M.P.; Caetano, H.V.A.; Visintin, J.A.; Assumpção, M.E.O.A. ................................................... 357

INFLUENCE OF EXOGENOUS DNA ON BOVINE SPERM CAPACITATION. Feitosa, W.B.;Milazzotto, M.P.; Simões, R.; Nicacio, A.C.; Nascimento, A.B.; Visintin, J.A.; Assumpção, M.E.O.A. ..... 358

EFFECT OF INHIBITION OF INDUCIBLE NITRIC OXIDE SYNTHASE ON MOTILITY ANDMEMBRANE INTEGRITY OF BOVINE IN VITRO CAPACITATED SPERMATOZOA. Ferreira-Berbari, J.B.P.; Caldas-Bussiere, M.C.; Paes de Carvalho, C.S.; Silva, A.E. ............................................... 359

EFFECTS OF VOLTAGE-DEPENDENT CALCIUM ION CHANNEL BLOCKERS ON MAMMALIANSPERMATOZOA IN IN VIVO AND IN VITRO FERTILIZATION Gabaldi, S.H.; Esper, C.R.; Tedesco,A.C.; Wolf, A.; Oliveira, J.A. ........................................................................................................................ 360

INFLUENCE OF ELECTROPORATION VOLTAGE ON BOVINE SPERMATOZOA VIABILITY FORIN VITRO EMBRYO PRODUCTION. Simões, R.; Binelli, M.; Nicacio, A.C.; Yamada, C.; Milazzotto,M.P.; Rovegno, M.; Visintin, J.A.; Assumpção, M.E.O.A. ........................................................................... 361

INFLUENCE OF THE BREED OF BULL AND THE BREED OF COW ON THE RESISTANCE OFBOVINE EMBRYOS TO HEAT SHOCK IN VITRO, AT EARLY STAGES OF DEVELOPMENT.Eberhardt, B.G.; Satrapa, R.A.; Capinzaiki, R.L.; Barros, C.M. .................................................................. 362

DIFFERENCES ON IN VITRO PRODUCTION OF EXPANDED BLASTOCYSTS, BLASTOCYSTSAND INITIAL BLASTOCYSTS OF 29 BULLS TESTED. Elias, F.P.; Fernandes, M.B.; Queiroz, L.F.M.;Vila, R.A.; Galerani, M.A.V.; Nomelini, J.; Lobo, R.B. ................................................................................ 363

BULL VARIATION ON IN VITRO EMBRYO PRODUCTION AND INTERACTION WITHDIFFERENT DONOR COWS. Accorsi, M.F.; Gonçalves, D.D.; Ferraz, M.L.; Watanabe, M.R.; Meirelles,F.D.P.; Meirelles, F.V.; Watanabe, Y.F. .......................................................................................................... 364

BULL EFFECT AT THE IN VITRO EMBRYO DEVELOPEMENT RATE DELIVERED FROM OVUMPICK-UP GUIDE BY ULTRASOUND. Morelli Júnior, J.; Atique Netto, H.; Martins, A.R.; Prado, R.B.;Fazio, F.L. ...................................................................................................................................................... 365

EFFECT OF FOLLICULAR ASPIRATION PRESSURE ON RECOVERY RATE AND CUMULUS-OOCYTE COMPLEX QUALITY IN GOATS AND EWES EXPLORED IN THE NORTH-EASTERNBRAZIL. Paula, N.R.O.; Magalhães, D.M.; Arruda, I.J.; Andrade, M.L.L.; Souza, A.L.; Pereira, A.F.;Almeida, K.C.; Avelar, S.R.G.; Silva, J.D.A.; Rondina, D.; Freitas, V.J.F. ................................................... 366

FOLLICULAR ASPIRATION IN GOAT FEMALES THROUGH LAPAROSCOPY TECHNIQUE.Cordeiro, M.F.; Vicente, W.R.R.; Di Filippo, P.A.; Alves, A.E.; Ribeiro, G.; Pavão, G.D.; Dias, D.P.M.;Dória, R.G.S.; Beretta, C.A.G.; Pereira, R.F.S.; Sousa, R.A.F. .................................................................... 367

EFFECT OF LH ON QUALITY OF OOCYTES OBTAINED BY OPU IN GUZERÁ BREED ANIMALS.Ueno, V.G.; Fontes, R.S.; Fonseca, F.A.; Gama Filho, R.V.; Bucher, C.H.; Ramos, J.L.G.; Carvalho,C.S.P.; Shimoya, A. ....................................................................................................................................... 368

XXII


EVALUATION OF FSH ADMINISTRATION BEFORE OVUM PICK-UP ON OOCYTES RECOVERYAND IN VITRO EMBRYO PRODUCTION IN NELORE (Bos taurus indicus) COWS. Nonato Junior,I.; Pontes, J.H.F.; Ereno Junior, J.C.; Sanches, B.V.; Seneda, M.M. ............................................................. 369

INFLUENCE OF HEAT STRESS ON BLASTOCYST RATE OF BOVINE EMBRYOS (Indicus vsTaurus) FROM OOCYTES OBTAINED BY OVUM PICK UP (OPU). Sartorelli, E.S.; Barcelos, A.C.;Satrapa, R.A.; Nogueira, M.F.G.; Martins, D.F.; Eberhardt, B.G.; Potiens, J.R.; Seneda, M.M.;Barros, C.M. .................................................................................................................................................. 370

USE OF COCONUT WATER SOLUTION AS PRESERVATION MEDIUM FOR THE TRANSPORTOF IMMATURE BOVINE OOCYTES. Cordeiro, M.S.; Silva, E.H.S.; Biondi, F.C.; Miranda, M.S.;Pauxis, D.R.; Santos, S.S.D.; Andreoti, M.; Ohashi, O.M............................................................................ 371

INFLUENCE OF THE YEAR SEASON IN THE OOCYTES PRODUCTION IN NELORE COWS.Rubin, K.C.P.; Max, M.C.; Pontes, J.H.F.; Nonato Jr., I.; Ereno Jr., J.C.; Uvo, S.; Sanches, B.V.; Rocha,J.L.; Seneda, M.M. ........................................................................................................................................ 372

IN VITRO MATURATION OF TRANSPORTED EQUINE OOCYTES. Jáuregui Lorda, M.; Herrera,C.; Pinto, M.; Losinno, L.; Miragaya, M.H. ................................................................................................. 373

DEVELOPMENTAL EVALUATION OF IN VITRO PRODUCED EMBRYOS FROM OOCYTESEXPERIMENTALY EXPOSED TO BOVINE HERPESVIRUS TYPE 1 (BHV-1) DURINGMATURATION. D’Angelo, M.; Athayde, C.S.; Souza, R.J.; Vieira, S.E.M.; Malaguti, J.M.A.; Rojas,N.; Melo, G.M.; Pavão, D.L. ......................................................................................................................... 374

NUCLEAR MATURATION EVALUATION OF OOCYTES FROM ESTRUS AND ANOESTRUSBITCHES USING TWO CULTURE MEDIA. Martins, L.R.; Ponchirolli, C.B.; Beier, S.L.; Landim-Alvarenga, F.C.; Lopes, M.D. ....................................................................................................................... 375

IN VITRO EMBRYO DEVELOPMENT FROM BOVINE OOCYTES MATURATED IN MEDIUMWITHOUT CONTROLLED GASEOUS ATMOSPHERE. Jasmin.; Polisseni, J.; Torres-Júnior, J.R.S.;Sá, W.F.; Camargo, L.S.; Ferreira, A.M.; Folhadella, I.M.; Viana, J.H.M.; Ramos, A.A. ........................... 376

STIMULUS OF GLUTATHIONE SYNTHESIS IN BOVINE OOCYTES DURING IN VITROMATURATION. Wolf, A.; Gabaldi, S.H.; Perecin, F.; Garcia, J.M. ............................................................ 377

INFLUENCE OF GLUTATHIONE SYNTHESIS DURING IN VITRO MATURATION OF BOVINEOOCYTES ON EMBRYONIC DEVELOPMENT AND QUALITY. Wolf, A.; Gabaldi, S.H.; Perecin, F.;Garcia, J.M. ................................................................................................................................................... 378

INFLUENCE OF VOLTAGE-DEPENDENT CALCIUM ION CHANNEL BLOCKERS ONMAMMALIAN OOCYTES IN IN VIVO AND IN VITRO FERTILIZATION. Gabaldi, S.H.; Esper, C.R.;Tedesco, A.C.; Wolf, A.; Oliveira, J.A. .......................................................................................................... 379

ANGIOTENSIN II AND ITS ASSOCIATION WITH INSULIN-LIKE GROWTH FACTOR-I, INSULINAND FOLLICULAR CELLS ON BOVINE OOCYTE MATURATION AND CONSEQUENT EMBRYODEVELOPMENT. Stefanello, J.R.; Arruda, J.N.T.; Zamberlam, G.O.; Oliveira, J.F.C.;Gonçalves, P.B.D. .......................................................................................................................................... 380

OXYGEN TENSION IN THE IVM AND IVF OF BOVINE OCYTES: EFFECT ON THE EMBRYONICDEVELOPMENT AND PREGNANCY RATE. Leivas, F.G.; Brum, D.S.; Saliba, W.P.; Alvim, M.T.T.;Santos, M.V.; Bernardi, M.L.; Rubin, M.I.B.; Silva, C.A.M. ....................................................................... 381

XXIII


IN VITRO MATURATION WITH LUNG AIR DURING TRANSPORT OF BOVINE OOCYTES.Mezzalira, A.; Mezzalira, J.C.; Bunn, S.; Werlich, D.E.; Paulini, F.; Wentz, K.C. ....................................... 382

EVALUATION OF MATURATION BLOCKING AND IN VITRO PIG EMBRYOS CULTURE IN PZM3AND NCSU23 MEDIA. Marques, M.G.; Nascimento, A.B.; Oliveira, V.P.; Coutinho, A.R.S.; Caetano,H.V.A.; Assumpção, M.E.O.A.; Visintin, J.A. ............................................................................................... 383

EVALUATION OF THE INFLUENCE OF GH, IGF-1 AND INSULIN ON BOVINE OOCYTE INVITRO MATURATION AND EMBRYO PRODUCTION. Ponchirolli, C.B.; Freitas, C.P.; Landim-Alvarenga, F.C. .............................................................................................................................................. 384

EFFECTS OF ANTIOXIDANTS AND CUMULUS CELLS ON IN VITRO MATURATION OF BOVINEOOCYTES. Gotardi, F.P.; Barretto, L.S.S.; Gonçalves, F.S.; Perri, S.H.V.; Mingoti, G.Z. ......................... 385

EFFECTS OF NUCLEAR MATURATION INHIBITORS AND ANTIOXIDANTS ON THE IN VITROMATURATION OF BOVINE OOCYTES. Barretto, L.S.S.; Gonçalves, F.S.; Perri, S.H.V.;Mingoti, G.Z. ................................................................................................................................................. 386

INFLUENCE OF GLUTATHIONE PRECURSORS DURING IN VITRO MATURATION (IVM) ONEMBRYONIC DEVELOPMENT OF BOVINE OOCYTES. Saraiva, N.Z.; Tetzner, T.A.D.; Borges,J.C.; Wolf, A.; Ferreira, C.R.; Perecin, F.; Méo, S.C.; Vantini, R.; Garcia, J.M. .......................................... 387

MITOCHONDRIA DISTRIBUTION IN BOVINE OOCYTES MATURED IN VITRO AFTER MEIOSISBLOCK. Pires, P.R.L.; Adona, P.R.; Quetglas, M.D.; Leal, C.L.V. ............................................................. 388

EFFECT OF MEIOSIS BLOCK ON CORTICAL GRANULE MIGRATION IN BOVINE OOCYTESMATURED IN VITRO. Adona, P.R.; Pires, P.R.L.; Quetglas, M.D.; Leal, C.L.V. ...................................... 389

EFFECT OF DIFFERENT BUTYROLACTONE I CONCENTRATIONS AND PRESENCE OF BSAON MEIOSIS BLOCK IN BOVINE OOCYTES. Adona, P.R.; de Bem, T.H.C.; Leal, C.L.V. ................... 390

GENE EXPRESSION IN BOVINE OOCYTES AND CUMULUS CELLS BEFORE AND AFTER INVITRO MATURATION WITH AND WITHOUT LH – PRELIMINARY RESULTS. Mundim, T.C.D.;Franco, M.M.; Brandão, D.O.; Ávila, F.F.; Melo, E.O.; Rumpf, R. ............................................................. 391

MICROFILAMENT DISTRIBUTION DURING IN VITRO MATURATION OF BOVINE OOCYTESAFTER MEIOSIS BLOCK. Adona, P.R.; Valério, J.; Leal, C.L.V. .............................................................. 392

MEIOSIS BLOCK OF BOVINE OOCYTES AND MICROTUBULE ORGANIZATION DURING INVITRO MATURATION. Pires, P.R.P.; Adona, P.R.; Leal, C.L.V. ................................................................ 393

MEIOSIS BLOCK WITH BUTYROLACTONE I DOES NOT INFLUENCE MITOCHONDRIAL DNACONTENT IN BOVINE OOCYTES IN VITRO. Biase, F.H.; Adona, P.R.; Miranda, M.S.; Leal, C.L.V.;Meirelles, F.V. ................................................................................................................................................ 394

ETHIDIUM BROMIDE IMPACT ON IN VITRO CYTOPLASMIC AND NUCLEAR MATURATIONOF BOVINE OOCYTES. Ferreira, C.R.; Meirelles, F.V.; Perecin, F.; Méo, S.C.; Tetzner, T.A.D.; Saraiva,N.Z.; Yamazaki, W.; Vantini, R.; Garcia, J.M. .............................................................................................. 395

DYNAMICS OF THE CONCENTRATION OF INTRACELLULAR GLUTATHIONE IN VITROMATURED BOVINE OOCYTES IN MEDIUM SUPPLEMENTED WITH PRECURSORS OFGLUTATHIONE. Tetzner, T.A.D.; Saraiva, N.Z.; Wolf, A.; Ferreira, C.R.; Perecin, F.; Borges, J.C.;Méo, S.C.; Vantini, R.; Garcia J.M. .............................................................................................................. 396

XXIV


IN VITRO PRODUCTION OF BUFFALO EMBRYOS – PRELIMINARY RESULTS. Ferraz, M.L.;Watanabe, Y.F.; Joaquim, D.C.; Watanabe, M.R.; Accorsi, M.F.; Meirelles, F.V.; Sá Filho, M.F.;Baruselli, P.S. ................................................................................................................................................. 397

EVALUATION OF MULTIPLEX-PCR SYSTEM USING INTERNAL CONTROL OF REACTIONTO SEX IDENTIFICATION OF IN VITRO PRODUCED BOVINE EMBRYOS. Rufino, F.A.; Wosiacki,S.R.; Féres, F.C.; Lisboa, L.A.; Seneda, M.M.; Alfieri, A.A. ....................................................................... 398

OPTIMIZATION OF THE MULTIPLEX-PCR FOR SEX IDENTIFICATION IN IN VITRO PRODUCEDBOVINE EMBRYOS. Rufino, F.A.; Wosiacki, S.R.; Lisboa, L.A.; Seneda, M.M.; Alfieri, A.A. ............... 399

CUMULUS-OOCYTE INTERACTION IN PROGRAMMED CELL DEATH IN BOVINE. Emanuelli,I.P.; Carambula, S.F.; Ripamonte, P.; Mesquita, L.G.; Biase, F.; Perna Junior, F.; Leal, C.L.V.; Santos,W.K.F.; Merighe, G.K.F.; Meirelles, F.V. ....................................................................................................... 400

KINETIC COMPETENCE OF COCS DURING IN VITRO CULTURE TO THE BLASTOCYST STAGE.Emanuelli, I.P.; Carambula, S.F.; Ripamonte, P.; Mesquita, L.G.; Perna Junior, F.; Cortezzi, S.S.; DeBem, T.H.C.; Merighe, G.K.F.; Leal, C.L.V.; Meirelles, F.V. ........................................................................ 401

COMMERCIAL IN VITRO PRODUCTION OF OVINE EMBRYOS. Watanabe, M.R.; Joaquim, D.C.;Meirelles, F.D.P.; Meirelles, F.V.; Watanabe, Y.F. ......................................................................................... 402

EFFECT OF HEPARIN IN BOVINE IN VITRO CULTURE ON DEVELOPMENTAL RATE AND SEXRATIO. Pegoraro, L.M.C.; Rheingantz, M.G.T.; Bredbacka, P.; Coscioni, A.; Rambo, G.; Abrahão, M.;Meirelles, M.; Saalfeld, M.H.; Agnonni, L. ................................................................................................... 403

INFLUENCE OF SUPERESTIMULATION AND HORMONAL DEPRIVATION PROTOCOLS ONIN VITRO PRODUCTION OF NELORE EMBRYOS (Bos taurus indicus). Satrapa, R.A.; Ferreira,M.M.G.; Ederhardt, B.G.; Monteiro, F.M.; Melo, D.S.; Potiens, J.R.; Barros, C.M. ................................... 404

PROGESTERONE AND OR ESTRADIOL INCREASES THE NUMBER OF CELLS IN BOVINE INVITRO PRODUCED BLASTOCYSTS. Reis, A.N.; Miranda, M.S.; Silva, A.O.A.; Sousa, A.J.O.; Silva,L.K.X.; Sousa, J.S.; Ohashi, O.M. ................................................................................................................ 405

BACTERIAL CONTAMINATION IN LIQUID NITROGEN CONTAINERS. Rubin, M.I.B.; Silva,C.A.M.; Oliveira, R.A.; Pessoa, G.A.; Navarro, R.B.; Pretto, A.N.; Vargas, A.C. ....................................... 406

ANALYSIS OF TRANSCRIPTS ASSOCIATED TO DE NOVO DNA METHYLATION ANDCHROMATIN STRUCTURE IN BOVINE BLASTOCYSTS. Camargo, L.S.A.; Powell, A.M.;Vale Filho, V.R.; Wall, R.J. ............................................................................................................................ 407

IN VITRO PRODUCED BOVINE EMBRYOS CULTURED IN CR2 MEDIUM SUPPLEMENTEDWITH KNOCKOUT™ SR. Serapião, R.V.; Ramos, A.A.; Polisseni, J.; Folhadella, I.M.; Jasmin.; Camargo,L.S.A1; Viana, J.H.M.; Ferreira, A.M.; Sá, W.F.; Fonseca, F.A. ................................................................... 408

CULTURE OF IN VITRO PRODUCED BOVINE EMBRYOS IN POLIVINYL ALCOHOL (PVA). Silva,D.S.; Pozzobon, S.E.; Navarro, R.B.; Melo, L.C.; Silva, C.A.M.; Rubin, M.I.B. ....................................... 409

EVALUATION OF DNA FRAGMENTATION AFTER FERTILIZATION IN BOVINE EMBRYOSMATURED IN VITRO IN MEDIA SUPPLEMENTED WITH DIFFERENT SOURCES OFMACROMOLECULES. Lo Turco, E.G.; Barretto, L.S.S.; Perecin, F.; Soria, G.F.; Bertolla, R.P.;Mingoti, G.Z. ................................................................................................................................................. 410

XXV


APOPTOSIS EVALUATION OF IN VITRO PRODUCED PIG EMBRYOS (PARTIAL RESULT).Coutinho, A.R.S.; Simões, R.; Marques, M.G.; Nascimento, A.B.; Oliveira, V.P.; Tavares, M.N.; Visintin,J.A.; Assumpção, M.E.O.A. ...........................................................................................................................411

CHROMOSOMAL ABNORMALITIES AT EARLY STAGES OF DEVELOPMENT IN ZEBUEMBRYOS PRODUCED IN VITRO. Pires, R.M.L.; Alvarez, R.H.; Ligori, A.C.; Coelho, L.A.;Martinez, A.C. ............................................................................................................................................... 412

EFFECT OF CR2aa, KSOMaa and SOFaa IN THE CULTURE OF IN VITRO PRODUCED BOVINEEMBRYOS. Tavares, L.M.T.; Feitosa, W.B.; Mello, M.R.B.; Lima, A.S.; Assumpção, M.E.O.A.;Visintin, J.A. .................................................................................................................................................. 413

CULTURE OF IN VITRO PRODUCED EMBRYOS IN SEMI-DEFINED MEDIA OR IN CO-CULTUREOF PRIMARY CELLS. Tavares, L.M.T.; Feitosa, W.B.; Magnusson, V.; Goissis, M.D.; Assumpção,M.E.O.A.; Visintin, J.A. ................................................................................................................................ 414

IN VITRO CULTURE OF BOVINE EMBRYOS IN SEMI-DEFINED MEDIA OR IN CO-CULTUREWITH CONTINOUS CELL LINE. Tavares, L.M.T.; Feitosa, W.B.; Caetano, H.V.A.; Assumpção,M.E.O.A.; Visintin, J.A. ................................................................................................................................ 415

EFFECT OF GLYCEROL AND ETHYLENE GLYCOL ON THE DEVELOPMENT OF IN VITROPRODUCED BOVINE EMBRYOS. Nicacio, A.C.; Yamada, C.; Simões, R.; Mendes, C.M.; Caetano,H.V.A.; Assumpção, M.E.O.A.; Visintin, J.A. ............................................................................................... 416

RESPIRATION BLOCKAGE OF IN VITRO BOVINE EMBRYOS. Mesquita, L.G.; Cortezzi, S.S.;Ripamonte, P.; Chiaratti, M.R.; Emanuelli, I.P.; De Bem, T.H.C.; Miranda, M.; Porciuncula, P.M.; Merighe,G.K.F.; Leal, C.L.V.; Meirelles, F.V. .............................................................................................................. 417

EVALUATION OF THE MALE: FEMALE RATIO IN THE IN VITRO PRODUCTION OF BOVINEEMBRYOS. Stranieri, P.; Accorsi, M.F.; Bishop, W.; Santos, R.G.; Watanabe, M.R.; Meirelles, F.D.P.;Meirelles, F.V.; Watanabe, Y.F. ...................................................................................................................... 418

BOVINE BLASTOCISTS PRODUCED AT CHEMICALLY DEFINED OR ORGANIC CULTURESYSTEMS. Soria, G.F.; Perecin, F.; Landim Jr., L.P.; Resende, M.V.; Meo, S.; Basso, A.; Paz, C.C.P.;Vantini, R.; Garcia, J.M.; Esper, C.R. ........................................................................................................... 419

QUALITY OF BOVINE BLASTOCYSTS CULTURED AT THE PRESENCE OR NOT OF PROTEINSSOURCES. Soria, G.F.; Landim Jr., L.P.; Resende, M.V.; Perecin, F.; Ferreira, C.R.; Basso, A.; Paz,C.C.P.; Vantini, R.; Garcia, J.M.; Esper, C.R. .............................................................................................. 420

DEVELOPMENT OF BOVINE BLASTOCYSTS AT CHEMICALLY DEFINED CULTURE MEDIA.Soria, G.F.; Basso, A.; Landim, L.P.; Resende, M.V.; Saraiva, N.Z.; Perecin, F.; Paz, C.C.P.; Vantini, R.;Garcia, J.M.; Esper, C.R. .............................................................................................................................. 421

EFFECT OF THE ESTRADIOL AND THE PROGESTERONE IN THE DEVELOPMENT OF BOVINEEMBRYOS IN VITRO PRODUCED. Reis, A.N.; Miranda, M.S.; Silva, A.O.A.; Sousa, A.J.O.; Silva,L.K.X.; Sousa, J.S.; Ohashi, O.M. ................................................................................................................ 422

USE OF SEQUENTIAL AND COMMERCIAL MEDIUM TO IN VITRO PRODUCTION OF BOVINEEMBRYOS: PRELIMINAR RESULTS. Zerbini, F.L.S.; Fernandes, M.B.; Elias, F.P.; Vila, R.A.; Galerani,M.A.; Lôbo, R.B. ........................................................................................................................................... 423

XXVI


IN VITRO BOVINE EMBRYO CULTURE IN PRESENCE OF GH, IGF-1 AND INSULIN. Ponchirolli,C.B.; Freitas, C.P.; Landim-Alvarenga, F.C. ................................................................................................. 424

EFFECT OF TRANSPORT ON DEVELOPMENT OF VITRIFIED-WARMED BOVINE EMBRYOS.Polisseni, J.; Ramos, A.A.; Sá, W.F.; Ferreira, A.M.; Camargo, L.S.; Folhadella, D.S.; Serapião, R.V.;Viana, J.H.M.; Nogueira, L.A.G. ................................................................................................................... 425

IN VITRO PRODUCED BOVINE EMBRYOS EXPOSED TO ETHYLENE GLYCOL ANDTREHALOSE. Mozzaquatro, F.D.; Franco, V.C.L.; Bunn, S.; Santos, M.V.; Silva, D.S.; Pilla, L.F.C.;Rubin, M.I.B.; Silva, C.A.M. ........................................................................................................................ 426

EFFICIENCY OF IMMATURE BOVINE OOCYTE VITRIFICATION. Yamada, C.; Nicacio, A.C.;Mendes, C.M.; Gonçalves, J.S.A.; Braga, F.A.; Assumpção, M.E.O.A; Visintin, J.A. ................................. 427

EFFECT OF MATURATION TIME ON CATTLE OOCYTE VITRIFICATION. Magnusson, V.; Yamada,C.; Tavares, L.M.T.; Nicacio, A.C.; Assumpção, M.E.O.A; Visintin, J.A. ................................................... 428

VIABILITY OF VITRIFIED BOVINE OOCYTES WITH TREHALOSE. Hastenreiter, G.P.; Dias, A.J.B.;Matos, L.F.; Carvalho, C.S.P. ........................................................................................................................ 429

EFFECT OF ADITION OF SUCROSE TO THE VITRIFICATION MEDIUM OF IN VITROPRODUCED ZEBU EMBRYOS. Varago, F.C.; Lagares, M.A.; Alvim, M.T.T.; Saliba, W.P.; Vasconcelos,A.B.; Elias, N.Q.; Stahlberg, R.; Oliveira, C.H. ............................................................................................ 430

PREGNANCY OF FRESH AND VITRIFIED BUFFALO EMBRYOS PRODUCED IN VITRO Sá Filho,M.F.; Carvalho, N.A.T.; Gimenes, L.U.; Torres-Júnior, J.R.; Ferreira, C.R.; Perecin, F.; Perini, A.P.;Tetzner, T.A.D.; Vantini, R.; Soria, G.F.; Garcia, J.M.; Tonhati, H.; Gasparrini, B.; Baruselli, P.S. ............ 431

EFFECT Of TIME BETWEEN THAWING AND FIXED TIME EMBRYO TRANSFER OF ETHYLENEGLYCOL FREEZED EMBRYOS. Peres, L.C.; Pincinato, D.; Borges, L.F.K.; Maraña Peña, D.; Tribulo,R.; Cutaia L.; Bó G.A. ................................................................................................................................... 432

THAWING EFFICIENCY OF OVINE EMBRYOS OF DORPER BREED. Andrade, J.C.O.; Sousa,B.P.A.; Guido, S.I. ......................................................................................................................................... 433

THE USE OF FLUORESCENT PROBES TO EVALUATE EQUINE CULTURED FIBROBLASTICOBTAINED FROM SKIN FRAGMENTS. Lima Neto, J.F.; Fernandes, C.B.; Landim-Alvarenga, F.C. ... 434

PRIMARY CULTURE STANDARDIZATION OF EQUINE FIBROBLAST FROM SKINFRAGMENTS. Fernandes, C.B.; Lima Neto, J.F.; Landim-Alvarenga, F.C. ............................................... 435

ANALYSIS OF THE CELL CYCLE OF GRANULOSA CELL CULTURE DERIVATED OFASPIRATED FOLLICLES OF NELORE CATTLE FOR NUCLEAR REPROGRAMMING. Caetano,H.V.A.; Schumacher, R.I.; Goissis, M.D.; Oliveira, V.P.; Feitosa, W.B.; Nicacio, A.C.; Gonçalves, J.S.A.;Assumpção, M.E.O.A.; Visintin, J.A. ............................................................................................................ 436

ANALYSIS OF THE CELL CYCLE OF ADULT AND FETAL FIBROBLASTS AS SOURCES FORNUCLEAR TRANSFER IN NELORE CATTLE. Caetano, H.V.A.; Schumacher, R.I.; Marques, M.G.;Yamada, C.; Simões, R.; Mendes, C.M.; Assumpção, M.E.O.A.; Visintin, J.A. ........................................... 437

CELL CYCLE ANALYSIS OF ADULT FIBROBLAST FOR NUCLEAR REPROGRAMMING INEQUINE. Caetano, H.V.A.; Schumacher, R.I.; Nascimento, A.B.; Martins, L.F.; Milazzotto, M.P.; Coutinho,A.R.S.; Assumpção, M.E.O.A.; Visintin, J.A. ............................................................................................... 438

XXVII


CHARACTERIZATION AND CELLULAR CYCLE ANALYSIS OF ADULT DOG FIBROBLAST(Great Dane). Caetano, H.V.A.; Schumacher, R.I.; Yamada, C.; Gonçalves, J.S.A.; Goissis, M.D.;Assumpção, M.E.O.A.; Visintin, J.A. ............................................................................................................ 439

EFFECTS OF FETAL CALF SERUM STARVATION OR CICLOHEXEMIDE TREATMENT ONPORCINE FETAL FIBROBLASTS. Goissis, M.D.; Caetano, H.V.A.; Marques, M.G.; Nascimento, A.B.;Oliveira, V.P.; Assumpção, M.E.O.A.; Visintin, J.A. ..................................................................................... 440

EFFECT OF PASSAGE NUMBER AND SEX OF FIBROBLASTS ON DEVELOPMENT OF BOVINENUCLEAR TRANSFER EMBRYOS. Merighe, G.K.F.; Miranda, M.S.; De Bem, T.H.C.; Watanabe,Y.F.; Meirelles, F.V. ....................................................................................................................................... 441

EVALUATION OF STRAWS (0.25mL) IN IN VITRO CULTURED BOVINE FIBROBLASTSCRYOPRESERVATION. De Bem, T.H.C.; Miranda, M.S.; Merighe, G.K.F.; Watanabe, Y.F.; Meirelles,F.V.; Ohashi, O.M. ......................................................................................................................................... 442

PRELIMINARY RESULTS OF BUFFALO CYTOPLASMIC TRANSFER TO BOVINE ZYGOTES.Ferreira, C.R.; Meirelles, F.V.; Sá Filho, M.F.; Baruselli, P.S.; Gasparrini, B.; Perecin, F.; Méo, S.C.;Tetzner, T.A.D.; Saraiva, N.Z.; Yamazaki, W.; Vantini, R.; Garcia, J.M. ..................................................... 443

PARTHENOGENETIC ACTIVATION OF BOVINE OOCYTES WITH CALCIUM IONOPHOREA23187 (CA) OR IONOMYCIN (IO). Milazzotto, M.P.; Feitosa, W.B.; Simões, R.; Martins, L.F.;Coutinho, A.R.S.; Assumpção, M.E.O.A.; Visintin, J.A. .............................................................................. 444

ACTIVATION AND PARTHENOGENETIC DEVELOPMENT OF BOVINE YOUNG OOCYTES.Biondi, F.C.; Cordeiro, M.S.; Miranda, M.S.; Santos, S.S.D.; Ohashi, O.M. .............................................. 445

PARTHENOGENETIC DEVELOPMENT OF BOVINE OOCYTES BLOCKED WITHBUTYROLACTONE I: PRELIMINARY RESULTS. Miranda, M.S.; Merighe, G.K.F.; Adona, P.R.;Biase, F.H.; Meirelles, F.V.; Leal, C.L.V. ...................................................................................................... 446

FFICIENCY OF CHEMICAL OR ELETRICAL ACTIVATION OF BOVINE OOCYTES Milazzotto,M.P.; Feitosa, W.B.; Martins, L.F.; Yamada, C.; Nicacio, A.C.; Assumpção, M.E.O.A.; Visintin, J.A. ....... 447

HATCHED RATES OF MICE EMBRYOS OF C57BL/6 AND C57BL/6 TRANSGENIC STRAINSAFTER IN VITRO CULTURE. Maffili, V.V.; Paiva, F.P.; Silva, C.A.; Viana, J.H.M. ................................ 448

BOVINE SPERM CAPACITATION USING CALCIUM IONOPHORE FOR IN VITRO PRODUCTIONOF TRANSGENIC EMBRYO. Simões, R.; Binelli, M.; Mendes, C.M.; Coutinho, A.R.S.; Feitosa, W.B.;Braga, F.A.; Visintin, J.A.; Assumpção, M.E.O.A. ....................................................................................... 449

PRONUCLEAR TRANSFER TO ACESS NUCLEAR-CYTOSPLASMIC INTERACTION BETWEENBOVINE OOCYTES FERTILIZED AND ACTIVATED WITH STRONTIUM. Méo, S.C.; Ferreira,C.R.; Perecin, F.; Yamazaki, W.; Leal, C.L.V.; Meirelles, F.V.; Garcia, J.M. ............................................... 450

SENSIBILITY EVALUATION OF MOUSE ZYGOTES TO Brucella abortus FOR ESTABLISHMENTOF AN EXPERIMENTAL MODEL TO PATHOGEN-EMBRYO INTERACTION STUDIES. Galuppo,A.G.; D’Angelo, M.; Miyashiro, S.; Scarcelli, E.; Senedezi, M.L.P.; Melo, G.M. ........................................ 451

CHARACTERIZATION OF THE TOP OF THE MATERNAL VILLI AND THE BASIS OF THE FETALVILLI IN ZEBÚ CATTLE (Bos indicus - Linnaeus, 1758). Pereira, F.T.V.; Cazerta, S.M.M.; Miglino,M.A.; Braga, F.C.; Meirelles, F.V.; Assis Neto, A.C.; Marques, R.S.; Vulcano, M. ..................................... 452

XXVIII


CHARACTERIZATION OF THE INTERCARUNCULAR REGION OF THE PREGNANT ZEBÚCOWS (Bos indicus – Linnaeus, 1758). Marques, R.S.; Miglino, M.A.; Braga, F.C.; Meirelles, F.V.;Assis Neto, A.C.; Vulcano, M.; Cazerta, S.M.M.; Pereira, F.T.V. ................................................................ 453

IMMUNOHISTOCHEMICAL STUDY OF THE TOP OF THE MATERNAL VILLI AND BASIS OFTHE FETAL VILLI REGION OF THE WATER BUFFALO PLACENTA (Bubalus bubalis bubalis -Simpson, 1945). Burioli, K.C.; Miglino, M.A.; Braga, F.C.; Bazer, F.W.; Kfoury Jr., J.R.; Oliveira, L.J.;Papa, P.C.; Oloris, S.C.S.; Dagli, M.L.Z.; Carvalho, A.F.; Pereira, F.T.V. .................................................. 454

EFFECT OF COX-1 AND 2- SELECTIVE INHIBITORS COTREATMENT ON FETALIMPLANTATION. Tomé, A.R.; Lima-Júnior, R.C.P.; Cunha, K.M.A.; Santos, F.A.; Rao, V.S.N. ............. 455

ANENCEPHALY AND GASTROSCHISIS BOVINE: A GROSS AND MICROSCOPICAL STUDY.Alberto, M.L.V.; Santos, T.C.; Assis Neto, A.C.; Matsumoto, F.S.; Miglino, M.A.; Ambrósio, C.E.;Mancanares, C.F.; Santos, J.M. ..................................................................................................................... 456

CYTOLOGIC TYPIFICATION OF THE AMNIOTIC FLUID FROM CALVES FORMED BY PIVAND NATURAL FECUNDATION AT THE DELIVERY MOMENT. Moya, C.F.; Prestes, N.C.;Piagentini, M.; Rocha, N.S. ........................................................................................................................... 457

BIOCHEMICAL ANALYSE OF AMNIOTIC FLUID OF NELORE CALVES FROM PIV ANDNATURAL FERTILIZATION COLLECTED AT DELIVERY MOMENT. Piagentini, M.; Prestes, N.C.;Moya, C.F.; Barretos, C.S.; Lucidi, C.A.; Takahira, R.K. ............................................................................ 458

EARLY IDENTIFICATION OF FETAL SEX AND DETERMINATION OF THE GENITAL TUBERCLEMIGRATION´S DAY IN DAIRY GOATS USING ULTRASOUND. Oliveira, M.A.L.; Santos, M.H.B.;Moraes, E.P.B.X.; Moura, R.T.D.; Chiamenti, A.; Bezerra, F.Q.G.; Rabelo, M.C.; Lima, P.F. .................... 459

QUANTITATIVE EVALUATION OF PROTEINS IN UTERINE FLUID OBTAINED DURING THEBOVINE ESTROUS CYCLE. Freire, A.F.; Lima, M.C.; Hucke, E.E.T.S.; Marques, V.B.; Miglino, M.A.;Binelli, M. ...................................................................................................................................................... 460

CANNULATION OF BOVINE UTERUS AS A METHOD TO STUDY REGIONAL FLUIDCOMPONENTS DURING EARLY PREGNANCY. Lima, M.C.; Freire, A.F.; Hucke, E.E.T.S.;Miglino, M.A.; Binelli, M. ............................................................................................................................. 461

IS ETHANOL LUTEOLYTIC IN COWS? de Barros, F.R.O.; Dias, L.O.P.; Fantini, D.A.; Ayres, G.R.;Marques, V.B.; Miguez, P.H.P.; Bertan, C.M.; Binelli, M. ............................................................................ 462

INCIDENCE OF HYDROMETRA AND MUCOMETRA IN GOATS. Moraes, E.P.B.X.; Santos, M.H.B.;Rocha, J.M.; Arruda, I. J.; Lima, P.F.; Oliveira, M.A.L. ............................................................................... 463

EFFECT OF UTERINE WASHINGS ON DURATION OF THE ESTROUS CYCLE IN CATTLE.Binelli, M.; Freire, A.F.; Lima, M.C.; Hucke, E.E.T.S.; Marques, V.B.; Miglino, M.A. .............................. 464

HISTOPHATOLOGICAL ASPECTS OF ADENOMYOSIS IN BOVINE UTERUS IN DIFFERENTPHASES OF ESTROUS CYCLE. Moreira, L.; Caldas-Bussiere, M.C.; Quirino, C.R.; Carvalho, E.C.Q. ....... 465

SEX STEROIDS MODULATE THE SYNTHESIS PGF2 ALPHA IN BOVINE ENDOMETRIALEXPLANTS. Miguez, P.H.P.; Da Cunha, P.M.; Marques, V.B.; Bertan, C.M.; Binelli, M. ......................... 466

XXIX


MODULATION OF PROSTAGLANDIN F2α PRODUCTION BY SERUM FACTORS IN CATTLE.

Dias, L.O.P.; de Barros, F.R.O.; Fantini, D.A.; Meneghini, R.C.M.; Pontes, E.O.; Bertan, C.M.; Miguez,P.H.P.; Binelli, M. .......................................................................................................................................... 467

QUANTITATIVE INHIBITION OF PROSTAGLANDIN-F2α SYNTHESIS: BASIS FOR A BIOASSAYTO MEASURE BOVINE INTERFERON-TAU ACTIVITY. Marques, V.M.; Bertan, C.M.; Merighe,G.K.F.; Meirelles, F.V.; Miglino, M.A.; Binelli, M. ....................................................................................... 468

EFFECT OF DIFFERENT PROGESTERONE CONCENTRATIONS DURING THE ESTROUSCYCLE IN ESTRADIOL 17β-INDUCED PROSTAGLANDIN-F2α PRODUCTION IN CATTLE.Fantini, D.A.; Goissis, M.D.; de Barros, F.R.O.; Dias, L.O.P.; Bressan, F.F.; Miguez, P.H.; Bertan, C.M.;Marques, V.B.; Almeida, A.B.; Binelli, M. ................................................................................................... 4 6 9

EFFECT OF THE USE OF D-CLOPROSTENOL AND OCITOCIN IN THE UTERINE INVOLTIONOF DAIRY COWS. Vasconcelos, R.S.; Rigo, A.G.; Lisboa, L.A.; Ribeiro, A.B.C.; Sgobero Filho, I.;Chacur, M.G.M.; Seneda, M.M. ................................................................................................................... 470

RELATIONSHIP OF THE INTRAUTERINE NITRITE CONCENTRATION WITH THEOCCURRENCE OF UTERINE ILLNESSES IN BOVINE. Moreira, L.; Viana, K.S.; Caldas-Bussiere,M.C.; Quirino, C.R.; Carvalho, E.C.Q. ......................................................................................................... 471

EFFECT OF GESTAGENS ON THE EXPRESSION OF STEROID RECEPTORS ON SHORT-LIFECORPUS LUTEUM AND ENDOMETRIUM IN POSTPARTUM ANESTROUS COWS. Loguercio,R.S.; Pimentel, C.A.; Loguercio, L.P.; Löf, H.K.; Fernandes, R.; Oliveira, J.F.C.; Gonçalves, P.B.D. ........ 472

ANALYSIS OF +15 REGION OF α-LACTALBUMIN GENE IN NELORE CATTLE (Bos primigeniusindicus). Martins, L.F.; Milazzotto, M.P.; Feitosa, W.B.; Mendes, C.M.; Nicacio, A.C.; Assumpção,M.E.O.A.; Visintin, J.A. ................................................................................................................................ 473

STUDY OF -1689 REGION OF α-LACTALBUMIN GENE IN NELORE CATTLE (Bos primigeniusindicus). Martins, L.F.; Milazzotto, M.P.; Caetano, H.V.A.; Feitosa, W.B.; Yamada, C.; Assumpção,M.E.O.A.; Visintin, J.A. ................................................................................................................................ 474

DIMETHYL FORMAMIDE AND COCONUT WATER IN CANINE SEMEN CRYOPRESERVATION.Zimmermann, M.; dos Santos, T.E.; Blume, H.; Snel-Oliveira, M.V.; Fidellis, A.A.G.; Mondadori, R.G. ... 475

STANDARDIZATION OF A DOUBLE STAIN USING TRYPAN BLUE PLUS GIEMSA TO EVALUATEDOG SPERM. Ribeiro, A.E.; Salavessa, C.M. ; Cunha, I.C.N.; Paes de Carvalho, C.S.; Silva, J.F.S. ....... 476

AUTHOR INDEX ....................................................................................................................................... 477

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INICIO ANAIS INGLES 2005 - Inicial — UFRGS Ingles - SBTE-2005 - Indice... · Marco Antonio Alvarenga Presidente 2004-2005. II Acta Scientiae Veterinariae 33(Supplement 1): 2005