Scientific Research ServiceProduct Catalogue

Ingenuity & Truth-seeking Assisting Scientific Research

General siRNA synthesisModified and fluorescently labeled siRNA synthesissiRNA design and screening

siRNAmicroRNA mimic inhibitor synthesismicroRNA agomir antagomirsynthesis

microRNA precursor synthesis

miRNAGeneral DNA synthesis

Modified and fluorescently labeled DNA synthesis

Aptamer/ASO/DNA probe synthesis

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DNA & RNA Primer andProbe Synthesis Service

Beijing Diagreat Biotechnologies Co., Ltd.

Beijing Diagreat Biotechnologies Co., Ltd.http://en.diagreat.com/

Add: Floor 1-3, Building 19, No. 2 Yard, Huanke Middle Road, Tongzhou District, Beijing, China.

International Sales Manager: Lydia ZhengWhatsapp/Mob: +86-13524602677Email: info@diagreat.com

As a new generation of molecular biology research tool, real-time fluorescent PCR has become a mainstream technology platform for molecular diagnosis, clinical testing and basic biomedical research. RT-PCR is widely used in molecular diagnosis of infectious diseases, tumors, genetic diseases, and personalized medicine.

Diagreat provides general primers, modified primers, labeled primers and dual-labeled fluorescent probes to meet the different requirements of biology, clinical diagnosis and drug development and other research fields, and also accepts special modification or labeled DNA synthesis.

Advantages

DNA Primer and Probe Synthesis Service Product List

Introduction

100,000-level Purification Workshop

The cleanliness level of the production environment is in accordance with GMP

requirements.

Strict Quality StandardsFive QC test items: appearance,

quantification, molecular weight, purity, fluorescence excitation/emission

wavelength, etc.

Customized and Industrialized Production

Providing large OD, g-level IVD exclusive high-purity primers and

ultra-pure primers.

Single-labeled probe

Dual-labeled probe

oligo-dT primer

Single-labeled probe

General primer

Classification DNA Length

Conventional Primer Synthesis

Explaination

11-59mer

All products are purified by HPLC

1 2

5'ROX 3'BHQ2

5' NH2 C6 5'ROX 3'MGB

NH2 C12 5'Cy5 3'BHQ2

3' NH2 C7 5'Cy5 3'BHQ3

5' -Phosphorylation 5'CY5 3'MGB

3' -Phosphorylation 5'Cy5.5 3'BHQ3

5' Biotin 5'TET 3'BHQ1

3' Biotin 5'TET 3'TAMRA

5' FAM 5'VIC 3'Dabcyl

3' FAM 5'VIC 3'BHQ1

5' HEX 5'VIC 3'TAMRA

5'TET 5'VIC 3'MGB

5' TAMRA dT-FAM

3' TAMRA dT-ROX

5' ROX dT-TAMRA

3' ROX dT-Dabcyl

5' Cy3 dT-Cy3

3' Cy3 dT-Cy5

5' Cy5 3'-Dabcyl

Cy5.5 5'-Dabcyl

3' Cy5 3' BHQ1

5' -VIC 3' BHQ2

3' BHQ3

3' eclipse

3'-MGB

Product type Modification type Product type Modification type

5- sulfhydryl

Product type Modification type Product type Modification type

For other modification types, please contact sales or customer service

RNA interference (RNAi) is an efficient and specific gene deletion research tool. It is a double-stranded RNA

(dsRNA)-mediated gene silencing phenomenon found in Andrew Fire and Craig Mello C. elegans. It almost exists in

all eukaryotes. RNAi has been widely used in gene function research of various eukaryotes, drug target discovery and

drug screening. In recent years, a variety of clinical trials have shown that RNAi drugs targeting disease-causing genes

have great potential to treat diseases that have plagued humans for many years.

Advantages

ISO 9001 strict quality control standards, providing a variety of purification methods and

comprehensive quality control reports (HPLC/MS)

The synthesis specifications are flexible and diverse, and can be customized in batches.

Provide personalized customized services such

as special services and preferential package

services.

Introduction

RNA Primer and Probe Synthesis ServiceLNA bp 5'FAM 3'TAMRA

dI (bp) 5'FAM 3'BHQ1

dU (bp) 5'FAM 3'Dabcyl

5'FAM 3'MGB

Spacer C3 (bp) 5'FAM 3'Eclipse

Spacer 9 (bp) 5'HEX 3'BHQ1

Spacer 12 (bp) 5'HEX 3'TAMRA

Spacer 18 5'HEX 3'Eclipse

dSpacer (bp) 5'HEX 3'Dabcyl

dT-C6-Amino (bp) 5'HEX 3'MGB

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Single-labeled probe Dual-labeled probe

Special Services

siRNA Design and SynthesisBased on the target gene name, sequence or GeneBank ID, etc, Diagreat carrys out free siRNA design to ensure that at least one pair can effectively inhibit the expression of target genes.The silent effect will be redesigned and synthesized for free.

siRNA screeningDiagreat provides effective siRNA screening services, helps customers screen out the most effective siRNA, and provides detailed experimental reports, including experimental methods, procedures, photos and related data.

Modified or labeled siRNA synthesisVarious modified and labeled siRNA synthesis can be carried out according to customer requirements. Custom modified or labeled siRNA synthesis is also available.

sufo (bp)

dI HPLC Spacer9 HPLC

rI HPLCAzide HPLC

dU HPLC DBCO HPLC

m5rC HPLC Spacer(C6) HPLC

m6rA HPLC Spacer9 HPLC

2'-O-Methyl HPLC Spacer(C12) HPLC

RNA HPLC Spacer C3 HPLC

2'F RNA HPLC Spacer C18 HPLC

m7rG HPLC dSpacer HPLC

LNA-X HPLC PL HPLC

m1rA HPLC Int Amino dT HPLC

m5Cm HPLC Int Biotin dT HPLC

m5Um HPLC Int Cy3 dT HPLC

Type Modification marker Purification method

Rare base

5 6

Other modification

Type Modification marker Purification method

2 OD

3 OD

4 OD

5 OD

6 OD

7 OD

8 OD

9 OD

10 OD

25 OD

50 OD

100 OD

250 OD

Product List

Product Type Specification Product Name Delivery

General siRNA General siRNA synthesis

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

5 working days

To be discussed

miRNA

2OD microRNA mimics

2OD microRNA inhibitor

2OD microRNA mimics N.C

2OD microRNA inhibitor N.C

2OD microRNA agomiR

2OD microRNA antagomiR

2OD microRNA agomiR N.C

2OD microRNA antagomiR N.C

Product Type Specification Product Name Delivery

5 working days

5 working days

5 working days

5 working days

11 working days

11 working days

11 working days

11 working days

(Phosphorothioates)S HPLC Int Cy5 dT HPLC

(Phosphorylation)5'PHO HPLC Int TAMRA dT HPLC

3' PHO HPLC Int ROX dT HPLC

(Amino Linkers) 5'Amino C6 HPLC Int FAM dT HPLC

3' Amino C7 HPLC Int HEX dT HPLC

SH modification3'Thiol C3 HPLC Int Dig dT HPLC

5' Thiol C6 HPLC Int DBCO dT HPLC

Biotin

5'Biotin HPLC Int TAMRA dT HPLC

3'Biotin TEG HPLC Int ATTO Flour dT HPLC

5'Desthiobiotin HPLCInt BHQ1 dT HPLC

3'Desthiobiotin HPLC Int BHQ2 dT HPLC

Digoxin5'DIG HPLC Int NPOM Dt HPLC

3' DIG HPLC

Quenchers

3'DABCYL HPLC

Fluorophore

5'Cy3 HPLC 3'TAMRA HPLC

3'Cy3 HPLC 3'BHQ 1 HPLC

5'Cy5 HPLC 3'BHQ 2 HPLC3'Cy5 HPLC 3'BHQ3 HPLC5'FAM HPLC 3'-MGB HPLC

3'FAM

HPLC

3'TQ1-TQ7 HPLC

5'HEX

HPLC

3'eclipse HPLC

3'HEX HPLC 5'Quasar 570 HPLC

5'-FITC HPLC 3'Quasar 570 HPLC

3'-FITC HPLC 5'Quasar 670 HPLC

5'6-JOE HPLC 3'Quasar 670 HPLC

3'6-JOE HPLC 5'Cy5.5NS HPLC

5'ROX HPLC 3'Cy5.5NS HPLC

3'ROX HPLC 5'Cy7NS HPLC

5'TAMRA HPLC 3'Cy7NS HPLC

3'TAMRA HPLC 5'&3'DABCYL HPLC

5'Texas Red HPLC 5'&3'AMCA HPLC

3'Texas Red HPLC 5'-VIC HPLC

TAMRA

5' FAM-3' TAMRA HPLC

ECLIPS

5' FAM-3' ECLIPS HPLC

5' HEX-3' TAMRA HPLC 5' HEX-3' ECLIPS HPLC

5' TET-3' TAMRA HPLC 5'TAMRA-3'Eclipse HPLC

5' JOE-3' TAMRA HPLC 5'ROX-3'Eclipse HPLC

5'VIC-3'TAMRA HPLC 5'TET-3'Eclipse HPLC

Human BHQ1

5' FAM-3' BHQ1 HPLC 5' JOE-3' Eclipse HPLC

5' VIC-3' BHQ1 HPLC

MGB

5' FAM-3'MGB HPLC

5' HEX-3' BHQ1 HPLC 5' HEX-3'MGB HPLC

BHQ2

5' Cy5-3' BHQ2 HPLC 5'ROX-3'MGB HPLC

5' Cy3-3' BHQ2 HPLC 5'Texas Red-3'MGB HPLC

5' ROX-3' BHQ2 HPLC 5'Quasar 670-3'MGB HPLC

5' TET-3' BHQ2 HPLC 5' VIC-3'MGB HPLC

5' JOE-3' BHQ2 HPLC

Dabcyl

5' FAM-3' DABCYL HPLC

5' HEX-3' BHQ2 HPLC 5' HEX-3' DABCYL HPLC

5' TAMRA-3' BHQ2 HPLC 5' TAMRA-3' DABCYL HPLC

5' Texas Red-3' BHQ2 HPLC 5'Texas Red-3'Dabcyl HPLC

5' Quasar 670-3' BHQ2 HPLC 5'ROX-3'Dabcyl HPLC

5' -FAM and 3' BHQ2 HPLC 5'CY3-3'DABCYL HPLC

BHQ35' Quasar 670-3' BHQ3 HPLC 5' Cy5-3' DABCYL HPLC

5' CY5-3' BHQ3 HPLC

5' Cy5,3' SH HPLC 5'Phos and 3'FAM HPLC

5' Dabcyl,3' C3-Fam HPLC 5'TAMRA and 3' Phos HPLC

5' DlG,3' Digoxin HPLC 5' C6-Biotin,3' Biotin HPLC

5' SH,3' Cy3 HPLC 5' C6-Biotin,3' Cy5 HPLC

5' Fam,3' C3-Fam HPLC 5' C6-NH2,3' C3-Fam HPLC

5' TAMRA 3' C3-Fam HPLC 5' C6-NH2,3' Cy3 HPLC

5' Cy5,3' C7-NH2 HPLC 5' Cy5,SS, HPLC

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Thiomodification

Phosphate group

NH2 modification

Fluorophore

Intermediate modification

Other double labeled modification

Other double labeled modification

For other modification types, please inquire about sales。

Type Modification marker Purification method Type Modification marker Purification method

7 8

Type Modification marker Purification method Type Modification marker Purification method