Infra Red Spectroscopy. Starter Activity. Hope you have your dancing shoes on…. I am going to play a selection of music and you just have to dance to

Infra Red Spectroscopy

Starter Activity.

• Hope you have your dancing shoes on….

• I am going to play a selection of music and you just have to dance to the music.

Infrared spectroscopy

A key characteristic of atoms, molecules and ions is that they can absorb EM radiation of specific frequencies – this can be used to identify them

Electromagnetic radiation absorbed

What the energy is used for

Spectroscopy technique

Ultra-violet / visibleMovement of electrons to higher energy levels

Ultra-violet / visible spectroscopy

Infra-red To vibrate bondsInfra-red

spectroscopy

Microwaves To rotate moleculesMicrowave

spectroscopy

Radio waves To change nuclear spin NMR spectroscopy

Infrared spectroscopy.Different covalent bonds have different strengths due to the masses of different atoms at either end of the bond.

As a result, the bonds vibrate at different frequencies

The frequency of vibration can be found by detecting when the molecules absorb electro-magnetic radiation.

Various types of vibration are possible.

Examples include... STRETCHING and BENDING

SYMMETRIC BENDING ASYMMETRIC STRETCHING STRETCH

SYMMETRIC STRETCHING

BENDING AND STRETCHING IN WATER MOLECULES

ASYMMETRIC STRETCHING



BENDING

• a beam of infra red radiation is passed through the sample

• a similar beam is passed through the reference cell

• the frequency of radiation is varied

• bonds vibrating with a similar frequency absorb the radiation

• the amount of radiation absorbed by the sample is compared with the reference

• the results are collected, stored and plotted

The Infra-red Spectrophotometer

A bond will absorb radiation of a frequency similar to its vibration(s)

The Infra-red Spectrophotometer

normal vibration vibration having absorbed energy

IDENTIFICATION OF PARTICULAR BONDSIN A MOLECULE

INFRA RED SPECTRA - USES

The presence of bonds such as O-H and C=O within a molecule can be confirmed because they have characteristic peaks in identifiable parts of the spectrum.

IDENTIFICATION OF COMPOUNDS BY DIRECT COMPARISON OF SPECTRA

The only way to completely identify a compound using IR is to compare its spectrum with a known sample. The part of the spectrum known as the ‘Fingerprint Region’ is unique to each compound.

Infra-red spectra are complex due to the many different vibrations taking place in each molecule.

INFRA RED SPECTRA - INTERPRETATION


Total characterisation of a substance based only on its IR spectrum is almost impossible unless one has computerised data handling facilities for comparison of the obtained spectrum with one in memory.




The technique is useful when used in conjunction with other methods -nuclear magnetic resonance spectroscopy and mass spectroscopy.




The technique is useful when used in conjunction with other methods -nuclear magnetic resonance spectroscopy and mass spectroscopy.

Peak position depends on bond strengthmasses of the atoms joined by the bond

strong bonds and light atoms absorb at lower wavenumbers weak bonds and heavy atoms absorb at high wavenumbers


Vertical axis Absorbance the stronger the absorbance the larger the peak

Horizontal axis Frequency wavenumber (waves per centimetre) / cm-1

Wavelength microns (m); 1 micron = 1000 nanometres


FINGERPRINT REGION

• organic molecules have a lot of C-C and C-H bonds within their structure• spectra obtained will have peaks in the 1400 cm-1 to 800 cm-1 range• this is referred to as the “fingerprint” region• the pattern obtained is characteristic of a particular compound the frequency of any absorption is also affected by adjoining atoms or groups.

IR SPECTRUM OF A CARBONYL COMPOUND

• carbonyl compounds show a sharp, strong absorption between 1700 and 1760 cm-1

• this is due to the presence of the C=O bond

IR SPECTRUM OF AN ALCOHOL

• alcohols show a broad absorption between 3200 and 3600 cm-1

• this is due to the presence of the O-H bond

IR SPECTRUM OF A CARBOXYLIC ACID

• carboxylic acids show a broad absorption between 3200 and 3600 cm-1

• this is due to the presence of the O-H bond• they also show a strong absorption around 1700 cm-1


IR SPECTRUM OF AN ESTER

• esters show a strong absorption between 1750 cm-1 and 1730 cm-1


WHAT IS IT!

O-H STRETCH

C=O STRETCH

O-H STRETCH

C=O STRETCH

AND

ALCOHOL

ALDEHYDE

CARBOXYLIC ACID

One can tell the difference between alcohols, aldehydes and carboxylic acids by comparison of their spectra.

O-H C=O C-O

N-H

Aromatic C-CC-H

C=C C-C alkanes

CN C-Cl

CHARACTERISTIC FREQUENCIES

Bond Class of compound Range / cm-1 Intensity

C-H Alkane 2965 - 2850 strongC-C Alkane 1200 - 700 weakC=C Alkene 1680 - 1620 variable

C=O Ketone 1725 - 1705 strongAldehyde 1740 - 1720 strongCarboxylic acid 1725 - 1700 strongEster 1750 - 1730 strongAmide 1700 - 1630 strong

C-O Alcohol, ester, acid, ether 1300 - 1000 strong

O-H Alcohol (monomer) 3650 - 3590 variable, sharpAlcohol (H-bonded) 3420 - 3200 strong, broadCarboxylic acid (H-bonded) 3300 - 3250 variable, broad

N-H Amine, Amide 3500 (approx) mediumCN Nitrile 2260 - 2240 medium

C-X Chloride 800 - 600 strongBromide 600 - 500 strongIodide 500 (approx) strong

CHARACTERISTIC ABSORPTION FREQUENCIES

Documents

Infra Red Spectroscopy. Starter Activity. Hope you have your dancing shoes on…. I am going to play a selection of music and you just have to dance to