Increased breast cancer risk in women with environmental exposure to pesticides

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<ul><li><p>S180 Abstracts / Toxicology Letters 196S (2010) S37S351</p><p>Amblyomin-X treatment (10or 100ng/10L)was topically appliedeach 48h in the absence or presence of B16F10 lineage, whichwas injected intomicrocirculatory beds of the subcutaneous tissue.Numbers of vessels and tumor growth were quantied in imagesobtained bemicrocirculence, amecAmblyominadded to thnumber of c(NO) weresignicantlmicrocirculparison to tin the absemigration iitored, indeand 100ng/Local appliber of micof endothesels formatthe mechanFAPESP gra</p><p>doi:10.1016</p><p>P203-023Effects of ton the hum</p><p>D. Pestana1</p><p>Monteiro1,</p><p>1 DepartmenUniversity oFaculty of ScInstituto SupPortugal</p><p>Human(POPs) islike dichmetabolitesdichlorodipbe particuldisruptingvery low din differentanisms is lehuman bein</p><p>In this rwere assess7). For thisinvasive poabsence ofmethyl-3H-the lactateusing a com</p><p>Cell prothese comwas observthe presenction was n(501000n</p><p>p,p-DDD. Additionally, all compounds showed cytotoxicity withdifferent concentrations (50 and 500nM p,p-DDD; 500 and1000nM p,p-DDE; 100 and 250nM o,p-DDT).</p><p>Cell invasionwas signicantly induced by all tested compounds,centre cygeth</p><p>xic anenicays hancedApoioD/64PD/4</p><p>.1016</p><p>24sed bre t</p><p>on1,</p><p>aciona, Spa</p><p>re toally ionsidntal cs. Thin Spposuundshaveta f2009ce Htwoaresha otal nale pesticises ipulaed rpestis stinat</p><p>f stud</p><p>.1016</p><p>25cannt le</p><p>on1,</p><p>aciona, Spa</p><p>lationeporfore and at 8th day after beginning of treatments. Afteratory endothelial cell lineage (T-end lineage) conu-hanic lesion in the culturewasdoneby a cell scraper and-X (10 or 100ng/well) or/and VEGF (100ng/mL) weree wells. Cell migration was monitored at 12h by theells migrating into the lesion and PGE2 and nitric oxidequantied in the supernatant. Results: Amblyomin-Xy reduced the number of vessels in the subcutaneousation (10ng=31.7% and 100ng/10L =42.7% in com-he rst day of treatment) and the tumor growth. Onlynce of VEGF, Amblyomin-X reduced endothelial cellnto the lesion focus during all period of time mon-pendently of PGE2 and NO secretion (10ng=16.4%10L =23.1% in comparison to the control). Discussion:cation of Amblyomin-X reduces, in vivo, the num-rocirculatory vessels and tumor growth. Impairmentlial cell migration may suggest an effect on new ves-ion. Future investigations will be carried out to clarifyisms involved in this process. Financial support: CAPES,nt 08/57850-8.</p><p>/j.toxlet.2010.03.612</p><p>he environmental pesticide DDT and its metabolitesan breast cancer cell line MCF-7</p><p>, D. Teixeira1, A. Faria1,2, V. Domingues3, R.C. Calhau1</p><p>t of Biochemistry (U38-FCT), Faculty of Medicine,f Porto, Portugal, 2 Chemistry Investigation Centre (CIQ),iences, University of Porto, Portugal, 3 Requimte erior de Engenharia, Instituto Politcnico do Porto,</p><p>exposure to persistent organic pollutantsa certainty, even to long banned pesticideslorodiphenyltrichloroethane (DDT), and its</p><p>dichlorodiphenyldichloroethylene (DDE) andhenyldichloroethane (DDD). POPs are known toarly toxic and have been associated with endocrine-effects in several mammals, including humans even atoses. The discovery that low concentrations, detectedhuman samples, may act on certain molecular mech-ading to a reassessment of metabolic POPs impact ongs.egard, the effects of o,p-DDT, p,p-DDE and p,p-DDDed on a hormone-sensitive breast cancer cell line (MCF-purpose, proliferation and viability in addition to theirtential were assayed, in the presence (501000nM) orthe tested compounds. Proliferation was evaluated bythymidine incorporation into DNA and viability troughdehydrogenase assay. Invasion potential was assessedmercial uorometric cell invasion assay.liferation and viability was not equally affected bypounds. A signicant decrease in cell proliferationed in the presence of o,p-DDT (100500nM). Ine of DDT metabolites, p,p-DDD, p,p-DDE, prolifera-ot signicantly affected for all tested concentrationsM), excepting a signicant reduction with 50nM of</p><p>in conincreas</p><p>Altocytotoestrogpathw</p><p>FindeSFRH/BSFRH/B</p><p>doi:10</p><p>P203-0Increaexposu</p><p>T. Parr</p><p>1 DelegAlmeri</p><p>Exposuespecibeen cronmemodelcancercide excompo</p><p>Welect da1998referened inof hect(&gt;1000</p><p>A toin femhigh p488 caThe poincreasof lowsion, thcontamtype o</p><p>doi:10</p><p>P203-0A liverdiffere</p><p>T. Parr</p><p>1 DelegAlmeri</p><p>The rebeen rations that were not able to decrease proliferation ortotoxicity.er, these results showed that these compounds wered able to induce cell invasion. Considering their known</p><p>activity, further research is needed to clarify the signalerein involved.</p><p>by FCT (POCI, FEDER, Programa Comunitrio, PTDC/QUI/65501/2006; SFRH/BD/46640/2008,691/2009, SFRH/BD/28160/2006 and0110/2007).</p><p>/j.toxlet.2010.03.613</p><p>reast cancer risk in women with environmentalo pesticides</p><p>R. Alarcon2, M.D.M. Requena1, A. Hernandez3</p><p>Provincial de Salud de Almeria, Spain, 2 Universidad dein, 3 Universidad de Granada, Spain</p><p>environmental pollutants has raised health concerns,n relation to its carcinogenicity effects. Pesticides haveered as risk factors for breast cancer since these envi-hemicals exert estrogenic activityon invitroand invivo</p><p>is fact, together with the increasing incidence of breastain and the lack of a clear relationship between pesti-re and this disease led us to evaluate the role of theseas a risk factor for breast cancer in women Almeria.performed a descriptive epidemiological study to col-</p><p>rom all cases of breast cancer for the study periodthrough thehospitalminimumdataset from the centralospital of Almeria province. This province was classi-different geographical areas according to the numberdevoted to intensive agriculture: high exposure areasf greenhouses) and low exposure areas (</p></li></ul>

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